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A study on the transmission of helicobacter pylorifrom food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples

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Health 2014年第4期6卷 274-283页

作者： Khawaja Shakeel Ahmed Basheer Madompoyil janak dulari ahi Aleem Ahmed Khan Santosh Kumar Tiwari Mohammed Aejaz Habeeb Asmara College of Health Sciences Department of Clinical Laboratory Sciences Microbiology Unit Asmara Eritrea Centre for Liver Research and Diagnostics Deccan College of Medical Sciences Hyderabad India Dr. Hari Singh Gour University Sagar Madhya Pradesh India Orotta School of Medicine and Dentistry Asmara Eritrea

Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in 1000 people across India. The Ratio of Male: female was 700: 300 with age group ranging from 20 - 39. Three gastric biopsies were collected for identification of Helicobacter pylori. PCR (Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis showed the prevalence of H. pylori as 70.8% in individuals frequently consuming food prepared under less hygienic condition (street vendor’s food) whereas 60% (less prevalence) in individuals consuming food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s food is not directly involved in the transmission of this bacterium. The unhygienic preparation of food and poor personal hygiene play a vital role in the transmission of these bacteria.

关键词： Helicobacter pylori Hygiene PCR 16SrRNA Food Biopsy

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Studying the Importance of VacAGene of Helicobacter pyloriin Identifying the Pathogenicity of Strains by Comparing It with the Disease Status of the Subjects

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Journal of Cancer Therapy 2013年第1期4卷 68-74页

作者： Khawaja Shakeel Ahmed Basheer Madompoyil Anghesom Ambesajir Ghebremedhin John Issac janak dulari ahi Aleem Ahmed Khan Santosh Kumar Tiwari Centre for Liver Research and Diagnostics Deccan College of Medical Sciences Hyderabad India Department of Clinical Laboratory Sciences Microbiology Unit Asmara College of Health Sciences Asmara Eritrea Dr. Hari Singh Gour University Sagar India.

Introduction: H. pylori is a primary pathogen isolated by Warren and Marshall in 1983. They called it as Campylobacter Pylori and in 1989 Goodwin et al. renamed it as Helicobacter pylori. Helicobacter pylorus is one of the responsible factors for causing gastritis, Peptic ulcer disease and is strongly associated with gastric carcinoma and gastric Malt lymphoma. Materials and Methods: In this study 300 biopsies were collected at Deccan College of Medical Sciences and Allied Hospitals, Hyderabad, India. Of these, 101 patients had peptic ulcer, 95 patients had gastritis and 4 had gastric carcinoma. A total of four gastric biopsy specimens were collected. One was used for culturing H. pylori, one for histological lesions and the remaining two one each from the antrum & corpus was collected in phosphate buffered saline for the DNA analysis. Results: Helicobacter pylori were isolated from many of the biopsies and the identification of Helicobacter pylori was confirmed in 200 biopsies with colony characteristics, Biochemical tests, and 16S rRNA amplification. The presence of VacA marker was detected by using appropriate primers. From the data obtained in our study, in a total of 169/200 isolates s1 region was found in 84.1% and s2 was found in 36/200 i.e. 18% of the isolates. In the middle region, m1awas found in 60/200 which was 30%, m1b was found in 60/200 i.e. 60% and m2 was found in 114/200 i.e. 57.1% of the isolates. Conclusion: Thus, VacA detection might be helpful for determination of which patients are at highest risk for severe clinical outcomes such as duodenal ulcer, gastric ulcer or gastric carcinoma and eventually, to define strategies for the treatment or prevention of H. pylori infection.

关键词： Helicobacter pylori 16S rRNA VacA Rapid Urease Test Culture

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