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Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice

Journal of Integrative Plant Biology 2009年第11期51卷 982-992页

作者： Shaohong Qu Jong-Seong Jeont Pieter B.F. Ouwerkerk Maria Bellizzi jan leach Pamela Ronald Guo-Liang Wang Department of Plant Pathology Ohio State University Columbus OH 43210 USA Institute of Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou 310021 China Department of Plant Pathology University of California Davis CA 95616 USA Graduate School of Biotechnology and Plant Metabolism Research Center Yongin 446-701 Korea Institute of Biology Leiden University Clusius Laboratory RA Leiden 2300 The Netherlands Bioagdcultural Sciences and Pest Management Colorado State University Fort Collins CO 80523 USA Crop Gene Engineering Key Laboratory of Hunan Province and Pre-State Key Laboratory of Crop Germplasm Renovation and Resource Utilization Hunan Agricultural University Changsha 410128 China

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches：（i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,

关键词： Ac-Ds transposable element glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain-inducible expression Cre-lox site-specific recombination rice.

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水稻受稻瘟菌侵染后发病初期的细胞学反应

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实验生物学报 2004年第5期37卷 344-350页

作者：杨民和郑重 jan E.leach 福建师范大学生物工程学院福州350007 浙江大学农业和生物技术学院植物保护系杭州310029 美国堪萨斯州立大学堪萨斯66506

采用致病性不同的3个稻瘟菌株接种水稻IR64品种。结果显示:在抗性反应中,病菌接种后水稻细胞中形成原生质颗粒,逐渐向病菌侵入部位聚集;原生质浓缩及沉积的形成、细胞坏死与病菌侵染菌丝的受抑制在时间和空间上是一致的。在中度抗性反应... 详细信息

采用致病性不同的3个稻瘟菌株接种水稻IR64品种。结果显示:在抗性反应中,病菌接种后水稻细胞中形成原生质颗粒,逐渐向病菌侵入部位聚集;原生质浓缩及沉积的形成、细胞坏死与病菌侵染菌丝的受抑制在时间和空间上是一致的。在中度抗性反应中,原生质颗粒化的时间延迟。在感病反应中,尚未观察到水稻细胞质浓缩现象。病菌侵染后寄主细胞在蓝光激发下的自发荧光表明,在寄主细胞中有多酚类物质的产生和积累。抗性反应中稻瘟菌接种后多酚类物质产生早,荧光范围大而强;中度抗性反应中,多酚类物质产生迟,荧光范围小而弱;而在感病反应中,难以观察到寄主细胞的自发荧光。胼胝质、磷脂酶D_γ(phospholipase D_γ,PLD_γ)产生和积累的趋势与多酚类积累的情况大致是相似的。不同互作类型寄主细胞中多酚类物质、胼胝质和PLD_γ产生和积累的差异表明,这些物质在水稻的抗病性中起了重要作用。

关键词：水稻稻瘟菌抗性反应病菌侵染寄主互作积累细胞接种后胼胝

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微管、微丝特异性抑制剂处理对水稻抗病性的影响

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植物病理学报 2003年第2期33卷 156-161页

作者：杨民和郑重 jan E.leach 江西农业大学农学院南昌330045 浙江大学植物保护系杭州310029 Kansas State University USA

微管特异性抑制剂oryzalin、微丝特异性抑制剂细胞松弛素A(cytochalasin A,CA)和细胞松弛素D(cytochalasin D,CD)的试验表明:oryzalin在5～50μmol/L、CA在0.5～1.0μg/mL、CD在1～20μg/mL的浓度范围内,对稻瘟病菌孢子的萌发和附着胞... 详细信息

微管特异性抑制剂oryzalin、微丝特异性抑制剂细胞松弛素A(cytochalasin A,CA)和细胞松弛素D(cytochalasin D,CD)的试验表明:oryzalin在5～50μmol/L、CA在0.5～1.0μg/mL、CD在1～20μg/mL的浓度范围内,对稻瘟病菌孢子的萌发和附着胞的形成基本上没有影响。采用以上几种细胞骨架特异性抑制剂处理水稻叶鞘都可以不同程度地抑制寄主细胞抗病菌扩展的能力。在抑制剂处理的水稻叶鞘细胞中,病菌扩展的速度加快。进一步的观察发现,抑制剂处理抑制水稻细胞抗病菌的扩展能力与水稻的抗病防卫反应如原生质颗粒化、多酚类物质的积累和HR发生的延迟是相关的。

关键词：微管特异性抑制剂微丝特异性抑制剂水稻抗病性叶鞘细胞细胞抗病菌原生质颗粒化多酚类物质

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以基因芯片辅助鉴定稻瘟病抗性相关基因

以基因芯片辅助鉴定稻瘟病抗性相关基因

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全国“植物生物技术及其产业化”研讨会

作者：刘燕朱小源张少红 Menchu Bernardo Jeremy Edward David Galbraith jan leach Hei Leung 刘斌 Plant Breeding Genetics and Biotechnology Division IRRILos BanosPhilippines 广东省农业科学院广东省农业科学院 Plant Breeding Genetics and Biotechnolosy Division IRRILos BanosPhilippines University of Arizona Tucson ArizonaUSA University of Arizona Tucson ArizonaUSA Colorado State University Fort Collins ColoradoUSA Plant Breeding Genetics and Biotechnology Division IRRILos BanosPhilippines 广东省农业科学院

＜正＞由真菌引起的稻瘟病是最具毁灭性的水稻病害。现代水稻品种的稻瘟病抗性周期短（1-3 年）是水稻生产中一个普遍而突出的问题。延长品种的稻瘟病抗性周期已成为水稻生产国急待解决的问题。实践表明,培育抗性品种、利用品种抗性是...

＜正＞由真菌引起的稻瘟病是最具毁灭性的水稻病害。现代水稻品种的稻瘟病抗性周期短（1-3 年）是水稻生产中一个普遍而突出的问题。延长品种的稻瘟病抗性周期已成为水稻生产国急待解决的问题。实践表明,培育抗性品种、利用品种抗性是最经济、安全、有效的稻瘟病防治途径。而深入了解水稻稻瘟病持久抗性分子遗传基础是稻瘟病持久抗性育种的技术关键。

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Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae *** and nitrogen deficiency

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Journal of Integrative Agriculture 2015年第5期14卷 888-899页

作者： YU Chao CHEN Hua-min TIAN Fang BI Yong-mei Rothstein J Steven leach E jan HE Chen-yang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Department of Molecular and Cellular Biology College of Biological ScienceUniversity of Guelph Department of Bioagricultural Sciences and Pest Management Colorado State University

Bacterial blight of rice caused by Xanthomonas oryzae ***（Xoo） is one of high nitrogen（N） responsive *** plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might ***,the co-regulatory components in such crosstalk have not been ***,we comparatively analyzed the gene expression profiling of rice under Xoo inoculation,low N treatment,or a combination of both stresses,and identified the differentially-expressed genes（DEGs） in overlapping *** DEGs were involved in different biological processes,including innate immunity and nitrogen *** randomly-selected DEGs expression was validated by quantitative real-time PCR *** expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were *** DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.

关键词： Xanthomonas differentially overlapping abiotic blight responsive crosstalk pathogen encoding validated

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Quantifying effects of soil heterogeneity on groundwater pollution at four sites in USA

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Science China(Life Sciences) 2005年第Z1期48卷 118-127页

作者： Saskia Vuurens Frank Stagnitti Gerrit de Rooij jan Boll Marc LeBlanc Daniel lerodiaconou Vince Versace Scott Salzman Wageningen University Department of Environmental SciencesSub-department Water ResourcesThe Netherlands School of Ecology and Environment Deakin UniversityWarrnambool CampusVic 3280Australia Department of Biological and Agricultural Engineering University of IdahoMoscowUSA Centre for Eco-EnvironmentalModeling Hohai UniversityNanjing 210098China

Four sites located in the north-eastern region of the United States of America have been chosen to investigate the impacts of soil heterogeneity in the transport of solutes(bromide and chloride)through the vadose zone(the zone in the soil that lies below the root zone and above the permanent saturated groundwater).A recently proposed mathematical model based on the cumulative beta distribution has been deployed to compare and contrast the regions'heterogeneity from multiple sample percolation *** differences in patterns of solute leaching were observed even over a small spatial scale,indicating that traditional sampling methods for solute transport,for example the gravity pan or suction lysimeters,or more recent inventions such as the multiple sample percolation systems may not be effective in estimating solute fluxes in soils when a significant degree of soil heterogeneity is ***,ignoring soil heterogeneity in solute transport studies will likely result in under-or overprediction of leached fluxes and potentially lead to serious pollution of soils and/or *** cumulative beta distribution technique is found to be a versatile and simple technique of gaining valuable information regarding soil heterogeneity effects on solute *** is also an excellent tool for guiding future decisions of experimental designs particularly in regard to the number of samples within one site and the number of sampling locations between sites required to obtain a representative estimate of field solute or drainage flux.

关键词： solutes drainage cumulative beta distribution pollution heterogeneity index bromide chloride leach-ing multiple sample percolation system preferential flow

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