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Journal of Biophysical Chemistry 2011年第2期2卷 137-146页

作者： jamie L. Kear Luis Galiano Angelo M. Veloro Laura S. Busenlehner Gail e. Fanucci Department of Chemistry University of Florida Gainesville Florida USA

Site-directed spin-labeling with continuous wave electron paramagnetic resonance spectroscopy was used to monitor autoproteolysis of HIV-1 protease, an enzyme essential for viral maturation. Two protein constructs were examined, namely subtype F and the circulating recombinant form CRF01_A/e. As the protease undergoes self-cleavage, protein unfolds and small peptide fragments containing the spin label are generated, which collectively give rise to a sharp spectral component that is easily discernable in the high-field resonance line in the ePR spectrum. By monitoring the intensity of this spectral component over time, the autoproteolytic stability of each construct was characterized under various conditions. Data were collected for samples stored at 4 °C, 25 °C, and 37 °C, and on a subtype F HIV-1 protease sample stored at 25 °C and containing the FDA-approved protease inhibitor Tipranavir. As expected, the rate of autoproteolysis decreased as the storage temperature was lowered. Minimal autoproteolysis was seen for the sample that contained Tipranavir, providing direction for future spectroscopic studies of active protease samples. w.e. compared to standard methods of monitoring protein degradation such as gel electrophoresis or chromatographic analyses, spin-labeling with Cw.e.R offers a facile, real-time, non-consuming way to monitor autoproteolysis or protein degradation. Additionally, mass spectrometry studies revealed that the N-termini of both constructs are sensitive to degradation and that the sites of specific autoproteolysis vary.

关键词： HIV-1 Protease Autoproteolysis Self-Proteolytic Activity Site-Directed Spin-Labeling electron Paramagnetic Resonance (ePR) Spectroscopy

Ⅰ期-Ⅲ期非转移性非小细胞肺癌的系统性治疗演变

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中国肺癌杂志 2021年第11期24卷 I0007-I0022页

作者： jamie e.CHAFT Andreas RIMNeR walter weDeR Christopher G.AZZOLI Mark G.KRIS Tina CASCONe 阎石(翻译) 吴楠(校对) Thoracic Oncology Service Department of MedicineMemorial Sloan Kettering Cancer CenterNew YorkNY and Weill Cornell Medical CollegeNew YorkNYUSA Department of Radiation Oncology Memorial Sloan Kettering Cancer CenterNew YorkNYUSA Thoracic Surgery Klinik Bethanien ZurichZurichSwitzerland Division of Hematology/Oncology Lifespan Cancer InstituteBrown UniversityProvidenceRIUSA Department of Thoracic/Head and Neck Medical Oncology The University of Texas MD Anderson Cancer CenterHoustonTXUSA These authors contributed equally:jamie e.Chaft Andreas Rimner 北京大学肿瘤医院暨北京市肿瘤防治研究所胸外二科恶性肿瘤发病机制及转化研究教育部重点实验室

早期肺癌患者的治疗是以治愈为目标的。针对手术可切除性和可操作性的多学科讨论模式决定了最终的局部治疗方式(手术或放疗)和相关的系统性治疗方案,从而进一步提高患者治愈的可能性。研究证据支持以顺铂为基础的辅助化疗用于切除术后,... 详细信息

早期肺癌患者的治疗是以治愈为目标的。针对手术可切除性和可操作性的多学科讨论模式决定了最终的局部治疗方式(手术或放疗)和相关的系统性治疗方案,从而进一步提高患者治愈的可能性。研究证据支持以顺铂为基础的辅助化疗用于切除术后,或与放疗同步使用。共识指南支持以新辅助化疗代替辅助化疗,并支持对不符合顺铂治疗条件的患者采用基于卡铂的治疗方案。由于研究设计效率低下,需要长时间随访来评估生存终点以及对晚期疾病的持续关注,将新药物(现在是IV期肺癌患者的标准药物)纳入以治愈为目标的治疗范式的工作一直滞后。目前正在研究中的替代性终点(例如病理缓解)将可能缩短研究的时间。2018年,抗程序性死亡配体(programmed cell death ligand 1,PD-L1)抗体度伐利尤单抗获批用于治疗同步放、化疗后的Ⅲ期肺癌患者,自那时起,针对早期肺癌患者的靶向治疗和免疫治疗的研究迅速发展。在本篇综述中,我们介绍了对于目前早期肺癌患者治疗方案的考虑因素,探讨并展望非转移性肺癌系统性治疗的临床研究现状和未来。

关键词：早期肺癌转移性肺癌系统性治疗新辅助化疗免疫治疗 death 靶向治疗局部治疗

Kicking behavior in response to a new rugby goalpost illusion

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Kicking behavior in response to a new rugby goalpost illusio...

Radiating Dyke Swarms in the BAT Region on Venus: A Study From the Helen Planitia Quadrangle

第十七届全国心理学学术会议

作者： jamie Poolton Rich Masters Gao Naichun Institute of Human Performance University of Hong Kong Department of Physical education Zhejiang University

In both rugby union and in rugby league,once a team scores a try,the scoring team attempts a conversion kick(worth two additional points),in which the designed kicker tries to kick the ball over the crossbar and through the *** kick can be taken from anyw.e.e along an imaginary line that runs parallel to the touch-line from w.e.e the ball was grounded for the *** this reason,it is useful to ground the ball as close to the goalposts as possible w.e. scoring a *** order to increase the chance of making the conversion attempt,the kicker is likely to move the ball back along the line to a position that maximizes the viewing angle betw.e. the uprights,but is within his or her kicking *** maximum kicking angle can be accurately calculated,using geometry,algebra and calculus,but this is obviously difficult to do in an actual rugby ***,kickers make their best guess of maximum kicking angle based upon their perception of the dimension of the ***,misperception of the dimensions of the goalposts,for instance the distancebetw.e. the uprights may influence the decision of w.e.e to take the conversion kick *** example,if the goalposts are perceived as narrower than they actually are the kicker may unnecessarily move further back to maximize the kicking ***,we presumed that if kickers perceived the rugby goalposts to be different,the ball placement would subsequently be affected by their pre-licking perceptual judgment.A series of studies were conducted to determine w.e.her a rugby goalposts illusion exists as a consequence of different heights of uprights,and to explore the relationship betw.e. uprights height and ball kicking position w.e. converting a *** experiment 1 and experiment 2,participants perceived the dimensions of the rugby goalposts(i.e.,crossbar length and crossbar height)differently for different heights of uprights,which suggested that a simple manipulation of uprights height creates a visual illusion

关键词： Rugby goalposts illusion Misperception Kicking behavior

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Acta Geologica Sinica(english edition) 2016年第S1期90卷 185-页

作者： jamie R.GRAFF Richard e.eRNST Claire SAMSON Department of earth Sciences Carleton University Faculty of Geology and Geography Tomsk State University

The Beta-Atla-Themis(BAT)region on Venus is characterized by large volcanic rises,major rift zones(chasmata),radiating,linear,and circumferential grabenfissure systems,and *** most prominent features

关键词： BAT A Study From the Helen Planitia Quadrangle Radiating Dyke Swarms in the BAT Region on Venus

An essential Oil Blend Decreases Methane emissions and Increases Milk Yield in Dairy Cows

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Open Journal of Animal Sciences 2019年第3期9卷 259-267页

作者： Kenton J. Hart Hefin G. Jones Kate e. waddams Hilary J. worgan Beatrice Zweifel C. jamie Newbold Institute of Biological Environmental and Rural Sciences (IBERS) Aberystwyth University Aberystwyth Ceredigion UK Caltech Crystalyx Siloth Cumbria UK Agolin SA Switzerland Scotland’s Rural College Kings Buildings Edinburgh UK

This study was conducted to investigate the effect of a commercial essential oil (eO) additive on milk production and methane (CH4) emissions from dairy cows. early lactation Holstein-Friesian dairy cows were fed grass, whole crop w.e.t and corn silage total mixed ration. Cows were allocated to one of two experimental treatments: Control (no additive, CON) or 1 g/head/day of eO. Cows were housed in a free stall barn, split into two pens for the duration of the experiment. Two gas data loggers units used to measure CH4 emissions were provided per pen for the duration of the 22 w.e.-long study. Milk yield was determined daily, and milk components were analyzed every two w.e.s. CH4 was recorded continuously, and daily values were tabulated. Body weight and body condition score were determined at the start and bi-w.e.ly. Results were analyzed as a randomized complete block trial. In total, 149 cows participated in the study (76 CON, 73 eO). Milk yields were greater (P e test treatment (28.3 CON, 31.2 eO) with no change in milk component concentrations. Milk component concentrations were unaffected (P > 0.05) by treatment. Yields of fat, protein, lactose, and solids were higher for eO fed cows (P 4 output was reduced with the eO compared to the CON treatment (411 g/day vs 438 g/day;13.8 g/L of milk vs 17.2 g/L of milk, P er the duration of the trial. There were no effects of treatment on reproductive performance or the occurrence of mastitis. Feeding eO to dairy cows reduced CH4 emissions whilst also increasing performance.

关键词： Dairy Cows essential Oils Methane Milk Yield

Mixed methods evaluation of a randomized control pilot trial targeting sugar-sw.e.ened beverage behaviors

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Open Journal of Preventive Medicine 2013年第1期3卷 51-57页

作者： jamie Zoellner emily Cook Yvonnes Chen wen You Brenda Davy Paul estabrooks Department of Agriculture and Applied economics Virginia Tech Blacksburg USA Department of Communications Virginia Tech Blacksburg USA Department of Human Nutrition Foods and exercise Virginia Tech Blacksburg USA

This excessive sugar-sw.e.ened beverage (SSB) consumption and low health literacy skills have emerged as two public health concerns in the United States (US);however, there is limited research on how to effectively address these issues among adults. As guided by health literacy concepts and the Theory of Planned Behavior (TPB), this randomized controlled pilot trial applied the Re-AIM framework and a mixed methods approach to examine a sugar-sw.e.ened beverage (SSB) intervention (SipSmarteR), as compared to a matched-contact control intervention targeting physical activity (Move-More). Both 5-w.e. interventions included two interactive group sessions and three support telephone calls. executing a patient-centered developmental process, the primary aim of this paper was to evaluate patient feedback on intervention content and structure. The secondary aim was to understand the potential reach (i.e., proportion enrolled, representativeness) and effectiveness (i.e. health behaviors, theorized mediating variables, quality of life) of SipS- marteR. Twenty-five participants were randomized to SipSmarteR (n = 14) or MoveMore (n = 11). Participants’ intervention feedback was positive, ranging from 4.2 - 5.0 on a 5-point scale. Qualitative assessments reavealed several opportuneties to improve clarity of learning materials, enhance instructions and communication, and refine research protocols. Although SSB consumption decreased more among the SipS-marteR participants (?256.9 ± 622.6 kcals), there were no significant group differences w.e. compared to control participants (?199.7 ± 404.6 kcals). Across both groups, there were significant improvements for SSB attitudes, SSB behavioral intentions, and two media literacy constructs. The value of using a patient-centered approach in the developmental phases of this intervention was apparent, and pilot findings suggest decreased SSB may be achieved through targeted health literacy and TPB strategies. Future efforts are needed to ex

关键词： Beverages Health Literacy Health education Public Health Health Behavior Pilot Projects

i-Tree eco Analysis of Landscape Vegetation on Remediated Areas of Oak Ridge National Laboratory

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Open Journal of Forestry 2020年第4期10卷 412-427页

作者： Sally Ross Sharon Jean-Philippe wayne Clatterbuck Neil Giffen jamie Herold John Zobel School of environment and Natural Resources The Ohio State University Columbus OH USA Department of Forestry Wildlife and Fisheries University of Tennessee Knoxville USA Oak Ridge National Laboratory Oak Ridge TN USA University of Minnesota Saint Paul MN USA

The Oak Ridge National Laboratory (ORNL) is the largest and most diverse energy, research, and development institution within the Department of energy (DOe) system in the United States. As such, the site endures constant land development that creates rigorous growing conditions for urban vegetation. Natural resource managers at ORNL recognize that trees are an integral component of the landscape and are interested in characterizing the urban forest and their associated ecosystem services benefits. we evaluated the urban forest structure, quantified ecosystem services and benefits, and estimated economic value of resources using i-Tree eco at ORNL. while this assessment captured over 1100 landscape trees, the ORNL Natural Resources Management for landscape vegetation can be expanded to include unmanaged landscapes, e.g. riparian areas, greenspace, and other vegetative attributes to increase ecosystem services benefits. Assigning a monetary value to urban forest benefits help to inform decisions about urban forest management, ideally on cost-benefit analysis.

关键词： Urban Tree(s) Urban Forest Urban Vegetation Landscape Vegetation Tree Inventory Tree Assessment ecosystem Service(s) ecological Function i-Tree eco economic Valuation

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中国第一家高端起泡酒领先品牌

Identifying contextual determinants of problems in tuberculosis care provision in South Africa: a theory-generating case study

新商务周刊 2015年第2期 52-54页

作者： jamie Lin

2013年6月28日,在宁夏回族自治区政府有关部门的牵头下,隶属于法国酩悦轩尼诗集团旗下,中国第一家高端起泡酒厂-酩悦轩尼诗夏桐(宁夏)酒庄在贺兰山下正式揭幕.这对宁夏贺兰山东麓葡萄酒产区登上国际舞台是一个重要里程碑19 8 7年,酩悦... 详细信息

2013年6月28日,在宁夏回族自治区政府有关部门的牵头下,隶属于法国酩悦轩尼诗集团旗下,中国第一家高端起泡酒厂-酩悦轩尼诗夏桐(宁夏)酒庄在贺兰山下正式揭幕.这对宁夏贺兰山东麓葡萄酒产区登上国际舞台是一个重要里程碑19 8 7年,酩悦轩尼诗(M o?t H e n n e s s y)与路易威登(L o u i s Vu it t o n)合并成LV M H(酩悦轩尼诗路易威登),奠定了其全世界最大的奢侈品集团的地位.旗下名闻遐迩的法国名酒酩悦香槟(Mo?t&Chandon)创立于1743年,已拥有超过270年的香槟酿酒历史.夏桐品牌起源于上世纪50年代,极赋先锋精神的酩悦酒庄于1959年在阿根廷门多萨建立了全球第一个夏桐酒庄,生产高端起泡葡萄酒.此

关键词：起泡酒轩尼诗宁夏回族自治区领先品牌贺兰山东麓酿酒历史先锋精神酿酒师门多萨路易威登

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Infectious Diseases of Poverty 2021年第3期10卷 82-94页

作者： jamie Murdoch Robyn Curran Andre Jvan Rensburg Ajibola Awotiwon Audry Dube Max Bachmann Inge Petersen Lara Fairall School of Health Sciences University of East AngliaNorwich NR47TJUK. University of Cape Town Lung Institute Knowledge Translation UnitUniversity of Cape TownMowbray 7700South Africa Centre for Rural Health University of KwaZulu NatalDurbanSouth Africa Norwich Medical School University of East AngliaNorwich NR47TJUK. King's Global Health Institute King's College LondonLondon SE19NHUK. Know.e.ge Translation Unit Department of MedicineUniversity of Cape Town Lung InstituteUniversity of Cape TownMowbray 7700South Africa.

Background:Despite progress towards end TB Strategy targets for reducing tuberculosis(TB)incidence and deaths by 2035,South Africa remains among the top ten high-burden tuberculosis countries globally.A large challenge lies in how policies to improve detection,diagnosis and treatment completion interact with social and structural drivers *** understanding and theoretical development of the contextual determinants of problems inTB care is required for developing effective *** article reports findings from the pre-implementation phase of a study ofTB care in South Africa,contributing to HeAlth System StrengThening in Sub-Saharan Africa(ASSeT)-a five-year research programme developing and evaluating health system strengthening interventions in sub-Saharan *** study aimed to develop hypothetical propositions regarding contextual determinants of problems inTB care to inform intervention development to reduce TB deaths and incidence whilst ensuring the delivery of quality integrated,person-centred ***:Theory-building case study design using the Context and Implementation of Complex Interventions(CICI)framework to identify contextual determinants of problems in TB *** February and November 2019,we used mixed methods in six public-sector primary healthcare facilities and one public-sector hospital serving impoverished urban and rural communities in the Amajuba District of KwaZulu-Natal Province,South *** data included stakeholder interviews,observations and documentary *** data included routine data on sputum testing andTB *** were inductively analysed and mapped onto the seven CICI contextual ***:Delayed diagnosis was caused by interactions betw.e. fragmented healthcare provision;limited resources;verticalised care;poorTB screening,sputum collection and *** nurse responsible forTB care,with limited integration ofTB with other conditions,and policy focused o

关键词： Tuberculosis Health systems strengthening Primary healthcare Person-centred care Context