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应用RAPD技术评价丁香品种间遗传关系

园艺学报 1995年第2期22卷 171-175页

作者：陈新露赵祥云 Bradley N White james s.pringle 北京农学院园林系加拿大ＭＣＭＡＳＴＥＲ大学生物系加拿大皇家植物园

采用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术分析了６个丁香（Ｓａｒｉｎａａ）品种间的遗传关系。用１７个单引物共扩增出８３个ＤＮＡ片段，其中清晰可辨、重复的片段６２个，占总数的７４．６％。被检测出品种特有标记，可作为区别不同... 详细信息

采用随机扩增多态ＤＮＡ（ＲＡＰＤ）技术分析了６个丁香（Ｓａｒｉｎａａ）品种间的遗传关系。用１７个单引物共扩增出８３个ＤＮＡ片段，其中清晰可辨、重复的片段６２个，占总数的７４．６％。被检测出品种特有标记，可作为区别不同品种的重要性状。品种ｆｌｏｒａ和ＮａｕｄＮｏｔｃｕｔｔ间相似系数高达９８．１％，证明它们是同一品种不同无性系。通过品种间相似系数比较，确认品种Ｒｏｃｈｅｓｔｅｒ是从品种Ｆｌｏｒａ选育而来；品种Ｍａｉｄｅｎ’ｓＢｌｕｓｈ是由朝鲜丁香（Ｓｙｒｉｎｇａｏｂｉａｔａｖａｒ．ｄｉｌａｔａｔａ）和欧丁香品种ＭｒｓＭａｒｒｙＢｉｃｋｌｅ杂交而来。

关键词：丁香品种 RAPD标记遗传关系

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RAPD标记在丁香分类鉴定中的应用

RAPD标记在丁香分类鉴定中的应用

A review of progress on nano-aperture VCsEL Invited Paper

中国科学技术协会第二届青年学术年会

作者：陈新露赵祥云 Bradley N．White james s．pringle 北京农学院园林系 McMaster大学生物系皇家植物园

本文采用RAPD技术对欧丁香(syringa vulgaris),s．x hyacinthiflora和s．x prestoniae等 10个品种进行分类鉴定研究。用17个引物共扩增出96个位点,平均每个引物产生6个位点,其中重现的多态位点和单态位点分别是46个(48．0％)和7个(7．2... 详细信息

本文采用RAPD技术对欧丁香(syringa vulgaris),s．x hyacinthiflora和s．x prestoniae等 10个品种进行分类鉴定研究。用17个引物共扩增出96个位点,平均每个引物产生6个位点,其中重现的多态位点和单态位点分别是46个(48．0％)和7个(7．2％),这些位点用作分类鉴定的分子行性状。用PAUP分析计算出品种间遗传距离,并获得品种间谱系树状图。研究表明,RAPD技术在丁香分类鉴定中是一种行之有效的方法。

关键词：丁香品种 RAPD标记分类鉴定谱系关系

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Chinese Optics Letters 2008年第10期6卷 748-754页

作者： sonny Vo james s.Harris solid state and Photonics Laboratory Stanford UniversityStanfordCalifornia94305USA

This paper reviews the progress on nano-aperture vertical-cavity surface-emitting lasers （VCsELs）. The design, fabrication, and polarization control of nano-aperture VCsELs are reviewed. With the nanoaperture evolving from conventional circular and square aperture to unique C-shaped, H-shaped, I-shaped, and bowtie-shaped aperture, both the near-field intensity and near-field beam confinement from nanoaperture VCsELs are significantly improved. As a high-intensity compact light source with sub-100- nm spot size, nano-aperture VCsELs are promising to realize many new near-field optical systems and applications.

关键词： VCsEL view A review of progress on nano-aperture VCsEL Invited Paper

Distinct MLT asymmetry of auroral kilometric radiation observed by the FAsT satellite

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Earth and Planetary Physics 2025年第1期9卷 188-193页

作者： JiaWen Tang sai Zhang FuLiang Xiao HongMing Yang si Liu YiHua He Chang Yang YuYue Jin ZhouKun Deng Ping Li Archie james Johnston school of Physics and Electronic sciences Changsha University of Science and TechnologyChangsha 410114China Hunan Province Higher Education Key Laboratory of Modeling and Monitoring on the Near-Earth Electromagnetic Environments Changsha University of Science and TechnologyChangsha 410114China school of Electrical and Information Engineering Changsha University of Science and TechnologyChangsha 410114China

Auroral Kilometric Radiation (AKR) is a common radio emission,which can contribute to the magnetosphere-ionosphereatmosphere co u *** emissions have been observed in all magnetic planet magnetospheres of the solar *** this study,using observations from the FAsT satellite from 30 August 1996 to 9 september 2001,the distribution of AKR in altitude=500-4500 km and invariant latitude (|ILAT|)=60°-80°has been analyzed.63045 AKR samples have been identified with~48%(52%) samples on the dayside (nightside).Of considerable interest,there is a distinct MLT asymmetry with the high occurrence rate in MLT=05-08 and 18-22(02-05 and 12-17) in the northern (southern) *** distinct MLT asymmetry is associated with the direction of Bxof the interplaneta ry magnetic *** addition,the occurrence rate on the nightside clearly increases as the AE^(*) index *** study further enriches the information and understanding of AKR in the magnetosphere as well as other similar radio emissions.

关键词： asymmetry auroral kilometric radiation interplanetary magnetic field

silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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Neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina Hromada Patrick Heimel Markus Kerbl LászlóGál sylvia Nürnberger Barbara schaedl james Ferguson Nicole swiadek Xavier Monforte Johannes C.Heinzel Antal Nógrádi Andreas H.Teuschl-Woller David Hercher Department Life science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria Ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University Clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tNGCs). However, the use of tNGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tNGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tNGCs and compared the various features of these improved silk^(+) tNGCs with the tubes without holes(silk^(–) tNGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tNGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tNGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tNGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tNGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

Treating amyotrophic lateral sclerosis with allogeneic schwann cell-derived exosomal vesicles: a case report

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Neural Regeneration Research 2025年第4期20卷 1207-1216页

作者： Pascal J.Goldschmidt-Clermont Aisha Khan George Jimsheleishvili Patricia Graham Adriana Brooks Risset silvera Alexander J.P.Goldschmidt Damien D.Pearse W.Dalton Dietrich Allan D.Levi james D.Guest Miller school of Medicine University of MiamiMiamiFLUSA stem Cell Institute Miller School of MedicineUniversity of MiamiMiamiFLUSA The Miami Project to Cure Paralysis Miller School of MedicineUniversity of MiamiMiamiFLUSA Alzady International LLC MiamiFLUSA Neurological surgery Miller School of MedicineUniversity of MiamiMiamiFLUSA

schwann cells are essential for the maintenance and function of motor neurons,axonal networks,and the neuromuscular *** amyotrophic lateral sclerosis,where motor neuron function is progressively lost,schwann cell function may also be ***,important signaling and potential trophic activities of schwann cell-derived exosomal vesicles have been *** case report describes the treatment of a patient with advanced amyotrophic lateral sclerosis using serial intravenous infusions of allogeneic schwann cell-derived exosomal vesicles,marking,to our knowledge,the first instance of such *** 81-year-old male patient presented with a 1.5-year history of rapidly progressive amyotrophic lateral *** initial diagnosis,the patient underwent a combination of generic riluzole,sodium phenylbutyrate for the treatment of amyotrophic lateral sclerosis,and *** patient volunteered to participate in an FDA-approved single-patient expanded access treatment and received weekly intravenous infusions of allogeneic schwann cell-derived exosomal vesicles to potentially restore impaired schwann cell and motor neuron *** confirmed that cultured schwann cells obtained from the amyotrophic lateral sclerosis patient via sural nerve biopsy appeared impaired(senescent)and that exposure of the patient’s schwann cells to allogeneic schwann cell-derived exosomal vesicles,cultured expanded from a cadaver donor improved their growth capacity in *** a period of observation lasting 10 weeks,during which amyotrophic lateral sclerosis Functional Rating scale-Revised and pulmonary function were regularly monitored,the patient received weekly consecutive infusions of 1.54×1012(×2),and then consecutive infusions of 7.5×1012(×6)allogeneic schwann cell-derived exosomal vesicles diluted in 40 mL of Dulbecco’s phosphate-buffered *** of the infusions were associated with adverse events such as infusion reactions(allergic or otherwise)or

关键词： allogeneic amyotrophic lateral sclerosis exosomes infusion neuromuscular junction schwann cell

Abiotic and biotic constituents of oil sands process-affected waters

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Journal of Environmental sciences 2023年第5期127卷 169-186页

作者： Nora A.s.Hussain james L.stafford Department of Biological sciences University of AlbertaEdmontonAlberta T6G 2N8Canada

The oil sands in Northern Alberta are the largest oil sands in the world,providing an important economic resource for the Canadian energy *** extraction of petroleum in the oil sands begins with the addition of hot water to the bituminous sediment,generating oil sands process-affected water(OsPW),which is acutely toxic to *** of litres of OsPW are stored on oil sands mining leased sites in man-made reservoirs called tailings *** the volume of OsPW increases,concerns arise regarding the reclamation and eventual release of this water back into the *** is composed of a complex and heterogeneous mix of components that vary based on factors such as company extraction techniques,age of the water,location,and bitumen ore ***,the effective remediation of OsPW requires the consideration of abiotic and biotic constituents within it to understand short and long term effects of treatments *** review summarizes selected chemicals and organisms in these waters and their interactions to provide a holistic perspective on the physiochemical and microbial dynamics underpinning OsPW.

关键词： OsPW toxicants Reclamation Tailings ponds Remediation Microbial AOPs Bioremediation

Current recommendations of managing HBV infection in preconception or pregnancy

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Frontiers of Medicine 2014年第2期8卷 158-165页

作者： james s. Park Calvin Pan Division of Gastroenterology Department of Medicine NYU Langone Medical Center NYU School of Medicine New York 10016 USA

Hepatitis B remains a leading cause of cirrhosis, hepatocellular carcinoma and liver transplantation worldwide. Management of chronic hepatitis B during pregnancy is challenging. Transmission of hepatitis B to infants still occurs perinataily although immunoprophylaxis is widely available for infants born to mothers with chronic hepatitis B infection. The emerging data suggest that initiation of antiviral therapy in the beginning of the third trimester in highly viremic mothers can prevent immunoprophylaxis failure in their infants. The available drug safety data show that lamivudine, telbivudine and tenofovir are generally safe to be used during the pregnancy. In order to minimize the fetal exposure to the antiviral medication, antiviral therapy during the pregnancy should be limited to a selected group of patients with cirrhosis, high hepatitis B viral load, or prior history immunoprophylaxis failure. An elective Caesarean section may reduce the risk of perinatal transmission. For those females planning for pregnancy or in early stage of pregnancy, communication and follow-up among obstetrician, gastroenterologist, and primary care physician are important. In this article, we will review the features of hepatitis B infection before, during and after the pregnancy; the risk factors that increase mother-tochild transmission; safety data on antiviral drug use during pregnancy; and the potential role of Caesarean section in selected cases.

关键词： antiviral therapy Caesarean section cirrhosis hepatitis B irnmunoprophylaxis mother-to-child transmission pregnancy prevention

Implicit Continuous User Authentication for Mobile Devices based on Deep Reinforcement Learning

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Computer systems science & Engineering 2023年第2期44卷 1357-1372页

作者： Christy james Jose M.s.Rajasree Government Engineering College Idukki685603India APJ Abdul Kalam Technological University Thiruvananthapuram695016India

The predominant method for smart phone accessing is conﬁned to methods directing the authentication by means of Point-of-Entry that heavily depend on physiological biometrics like,ﬁngerprint or *** continuous authentication initiating to be loftier to conventional authentication mechanisms by continuously conﬁrming users’identities on continuing basis and mark the instant at which an illegitimate hacker grasps dominance of the ***,divergent issues remain *** research aims to investigate the power of Deep Reinforcement Learning technique to implicit continuous authentication for mobile devices using a method called,Gaussian Weighted Cauchy Kriging-based Continuous Czekanowski’s(GWCK-CC).First,a Gaussian Weighted Non-local Mean Filter Preprocessing model is applied for reducing the noise pre-sent in the raw input face *** Kriging Regression function is employed to reduce the ***,Continuous Czekanowski’s Clas-siﬁcation is utilized for proﬁcient classiﬁcation between the genuine user and *** this way,the proposed GWCK-CC method achieves accurate authen-tication with minimum error rate and *** assessment of the pro-posed GWCK-CC method and existing methods are carried out with different factors by using UMDAA-02 Face *** results conﬁrm that the proposed GWCK-CC method enhances authentication accuracy,by 9%,reduces the authen-tication time,and error rate by 44%,and 43%as compared to the existing methods.

关键词： Deep reinforcement learning gaussian weighted non-local meanﬁlter cauchy kriging regression continuous czekanowski’s implicit continuous authentication mobile devices