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Plant Carbonic Anhydrases： Structures, Locations, Evolution, and Physiological Roles

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Molecular Plant 2017年第1期10卷 30-46页

作者： Robert j. DiMario Harmony Clayton Ananya Mukhedee Martha Ludwig james V. Moroney Department of Biological Sciences Louisiana State University Baton Rouge LA 70803 USA School of Chemistry and Biochemistry University of Western Australia Perth WA 6009 Australia

Carbonic anhydrases （CAs） are zinc metalloenzymes that catalyze the interconversion of CO2 and HCO3- and are ubiquitous in nature. Higher plants contain three evolutionarily distinct CA families, αCAs, 13CAs, and γCAs, where each family is represented by multiple isoforms in all species. Alternative splicing of CA transcripts appears common; consequently, the number of functional CA isoforms in a species may exceed the number of genes. CAs are expressed in numerous plant tissues and in different cellular locations. The most prevalent CAs are those in the chloroplast, cytosol, and mitochondria. This diversity in location is paralleled in the many physiological and biochemical roles that CAs play in plants. In this review, the number and types of CAs in C3, C4, and crassulacean acid metabolism （CAM） plants are considered, and the roles of the α and yCAs are briefly discussed. The remainder of the review focuses on plant βCAs and includes the identification of homologs between species using phylogenetic approaches, a consideration of the inter- and intracellular localization of the proteins, along with the evidence for alternative splice forms. Current understanding of βCA tissue-specific expression patterns and what controls them are reviewed, and the physiological roles for which βCAs have been implicated are presented.

关键词： carbonic anhydrase regulation alternative splicing physiological role

Gold modified cobalt-based Fischer-Tropsch catalysts for conversion of synthesis gas to liquid fuels

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Frontiers of Chemical Science and Engineering 2013年第3期7卷 262-269页

作者： Alan j. Mccue jura Aponaviciute Richard P.K. Wells james A. Anderson Surface Chemistry and Catalysis Group Department of Chemistry University of Aberdeen AB24 3UE Abcrdccn UK Surface Chemistry and Catalysis Group School of Engineering University of Aberdeen AB24 3UE Aberdeen UK

The addition of Au as a promoter/modifier for alumina supported Co catalyst has been studied by combined in-situ high temperature, high pressure Fourier transform infrared （FTIR） and on-line gas chromatography. The combination of these tools permitted the state of the active catalyst surface to be monitored while following the elution of reaction products during the first 5-7 h on stream of the catalyst. The catalysts under study were a 10%Co/ A1203 and a 2.5%Au/10%Co/A1203. Samples were characterised before use using Raman and temperature programmed reduction （TPR）. During the initial stages of reaction, hydrocarbons were built up on the surface of the catalyst as monitored by FTIR and the nature and amount of these species were assessed in terms of CH2/CH3 ratio and the density of these alkyl fragments by employing absorption coefficients for the individual components. The nature and reducibility of the Co particles were modified by the presence of Au while the later also shifted the CO/ H2 balance by acting as an effective water-gas shift catalyst during the early stages of reaction. This characteristic was lost during reaction as a consequence of redistribution of the two metallic phases.

关键词： gold modified catalyst conversion of synthesis gas

Endophytic and pathogenic fungi of developing cranberry ovaries from flower to mature fruit:diversity and succession

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Fungal Diversity 2012年第3期54卷 101-116页

作者： Mariusz Tadych Marshall S.Bergen jennifer johnson-Cicalese james j.Polashock Nicholi Vorsa james F.White jr Department of Plant Biology and Pathology Rutgers University59 Dudley RoadNew BrunswickNJ 08901USA Philip E.Marucci Center for Blueberry and Cranberry Research and Extension Rutgers University125A Lake Oswego RoadChatsworthNJ 08019USA Genetic Improvement of Fruits and Vegetables Laboratory USDA-ARS125A Lake Oswego RoadChatsworthNJ 08019USA

Culturable fungal population diversity and succession was investigated in developing cranberry ovaries of fruit rot-resistant and rot-susceptible cranberry selections,from flower through mature *** were recovered in culture from 1185 of 1338 ovary tissues collected from june to September,yielding 2255 isolates that represented 42 *** the season,species richness varied from 2 to 17 and 2 to 18 in rot-resistant and rotsusceptible selections,respectively,increasing from wk1 to wk10 and then gradually declining to ***-Wiener diversity index varied from 0.27 to 2.32 in rotresistant and 0.18 to 2.38 in rot-susceptible,and Pielou’s evenness index varied from 0.11 to 0.63 and 0.06 to 0.64 in rot-resistant and rot-susceptible selections,respectively,confirming that diversity of fungi in developing ovaries was similar among rot-resistant and rot-susceptible selections,but varied among sampling time *** component analysis grouped samples collected at the same sampling time point together regardless of rot susceptibility of the selections,and detected the predominant fungal species associated with each stage of *** changes were observed in populations of endophytic,pathogenic and saprophytic fungi throughout the season as ovaries matured.

关键词： Aetiology Cranberry fruit rot Pathogens Phenology Resistance Vaccinium macrocarpon

Primary care clinicians’ strategies to overcome financial barriers to specialty health care for uninsured patients

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Family Medicine and Community Health 2015年第3期3卷 18-24页

作者： james j.Werner Kitty K.Corbett Department of Family Medicine and Community Health Mandel School of Applied Social SciencesCase Western Reserve University11000 Cedar AvenueSuite 402ClevelandOH 44106USA School of Public Health and Health Systems University of Waterloo200 University Avenue WestWaterlooOntarioN2L 3G1Canada

Objective:This study describes strategies used by federally qualified health centers(FQHCs)to assist medically uninsured patients in obtaining specialty health care ***:Qualitative methods were used to study strategies for obtaining specialty health care for uninsured *** were gathered from 10 primary care clinicians at three FQHC clinics by means of 10 semistructured interviews,23 brief interviews,and 45 h of direct *** captured additional data by studying cases of referred uninsured ***:The following six strategies were identified:(1)quid pro quo-a specialist accept-ing the clinic’s medically uninsured patients was rewarded with referrals of the clinic’s insured patients;(2)over referral-clinicians referred insured patients whose needs could have been met at the FQHC;(3)brief hospitalization-when a specialist could not be obtained,high-risk patients were briefly hospitalized;(4)case building-diagnostic tests were conducted at the FQHC to justify a referral;(5)direct communication-communication between clinicians and specialists was neces-sary when requesting a referral;(6)specialty clinics-in return for conducting a specialty clinic at the FQHC,the specialist received all referrals of insured ***:Uninsured FQHC patients encountered difficulties accessing specialty health care,and in response,clinicians developed a range of innovative strategies.

关键词： Health services accessibility barriers to health care medically uninsured health care inequities access to specialty care qualitative methods

Effects of rock pore sizes on the PVT properties of oil and gas-condensates in shale and tight reservoirs

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Petroleum 2018年第2期4卷 148-157页

作者： Arash Kamari Lei Li james j.Sheng Bob L.Herd Department of Petroleum Engineering Texas Tech UniversityP.O.Box 43111LubbockTX 79409United States Southwest Petroleum University ChengduChina

Shales make up a large proportion of the rocks with extremely low permeability representing many challenges which can be complex in many cases.A careful study of rock and fluid properties(*** of shales)of such resources is needed for long-term success,determining reservoirs quality,and increased recovery factor in unique unconventional *** this communication,a comprehensive thermodynamic modelling is undertaken in which capillary pressure is coupled with the phase equilibrium *** this end,the data associated with both shale oil and gascondensates of Eagle Ford shale reservoir located in South Texas,U.S.,is *** properties,including bubble and dew point pressures,capillary pressure,interfacial tension,liquid and gas densities,and liquid and gas viscosities,are predicted observing the effects of rock pore sizes by the thermodynamic modelling performed in this *** results demonstrate that the thermodynamic model developed in this study is capable of simulating the PVT properties of oil and gascondensates in shale and tight *** a binary mixture 25:75 C1/C6,the bubble point pressure at different reservoir temperature is increased by increasing the pore sizes from 10 to 50 ***,an increase in pore sizes from 10 to 50 nm can increase the dew point pressure for a studied binary mixture 75:25 C1/C6.

关键词： Shale Pore size Oil Gas-condensate Thermodynamic modelling

Compressing local atomic neighbourhood descriptors

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npj Computational Materials 2022年第1期8卷 1593-1605页

作者： james P.Darby james R.Kermode Gábor Csányi Engineering Dept University of CambridgeTrumpington StCambridgeCB21PZUK Warwick Centre for Predictive Modelling School of EngineeringUniversity of WarwickCoventryCV47ALUK

Many atomic descriptors are currently limited by their unfavourable scaling with the number of chemical elements S *** length of body-ordered descriptors,such as the SOAP power spectrum(3-body)and the(ACE)(multiple body-orders),scales as(NS)^(ν)whereν+1 is the body-order and N is the number of radial basis functions used in the density *** introduce two distinct approaches which can be used to overcome this scaling for the SOAP power ***,we show that the power spectrum is amenable to lossless compression with respect to both S and N,so that the descriptor length can be reduced from O(N^(2)S^(2))to O(NS).Secondly,we introduce a generalised SOAP kernel,where compression is achieved through the use of the total,element agnostic density,in combination with radial *** ideas used in the generalised kernel are equally applicably to any other body-ordered descriptors and we demonstrate this for the(ACSF).

关键词： SOAP scaling kernel

Adiponectin signalling in bone homeostasis,with age and in disease

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Bone Research 2021年第1期9卷 15-25页

作者： jonathan W.Lewis james R.Edwards Amy j.Naylor Helen M.McGettrick Rheumatology Research Group Institute of Inflammation and AgeingUniversity of BirminghamBirmingham B152TTUK Ageing&Regeneration Research Group Botnar Research CentreUniversity of OxfordOxford OX37LDUK

Adiponectin is the most abundant circulating adipokine and is primarily involved in glucose metabolism and insulin *** the bone,osteoblasts and osteoclasts express the adiponectin receptors,however,there are conflicting reports on the effects of adiponectin on bone formation and *** studies have shown a pro-osteogenic role for adiponectin in in vivo murine models and in vitro:with increased osteoblast differentiation and activity,alongside lower levels of ***,human studies often demonstrate an inverse relationship between adiponectin concentration and bone ***,the presence of multiple isoforms of adiponectin and multiple receptor subtypes has the potential to lead to more complex signalling and functional *** such,we still do not fully understand the importance of the adiponectin signalling pathway in regulating bone homeostasis and repair in health,with age and in *** this review,we explore our current understanding of adiponectin bioactivity in the bone;the significance of its different isoforms;and how adiponectin biology is altered in ***,furthering our understanding of adiponectin regulation of bone biology is key to developing pharmacological and non-pharmacological(lifestyle)interventions that target adiponectin signalling to boost bone growth and repair in healthy ageing,following injury or in disease.

关键词： homeostasis metabolism consequences

Double-blind randomized sham controlled trial of intraperitoneal bupivacaine during emergency laparoscopic cholecystectomy

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Hepatobiliary & Pancreatic Diseases International 2013年第3期12卷 310-316页

作者： Keith j Roberts jeff Gilmour Ruplay Pande james Hodson For Tai Lam Saboor Khan Department of Hepatopancreatobiliary Surgery University Hospitals Coventry and Warwickshire Wolfson Laboratory University Hospitals Birmingham

BACKGROUND: Intraperitoneal local anesthesia (IPLA) during elective laparoscopic cholecystectomy (el-LC) decreases post-operative pain. None of the studies have explored the efficacy of IPLA at emergency laparoscopic cholecystectomy (em-LC). A longer operative duration, the greater frequency of washing, and the inflammation associated with cholecystitis or pancreatitis are a few reasons why it cannot be assumed that a benefit in pain scores will be seen in em-LC with IPLA. This study was undertaken to assess the efficacy of IPLA in patients undergoing em-LC. METHODS: Double-blind randomized sham controlled trial was conducted of 41 consecutive subjects undergoing em-LC. IPLA was delivered by a combination of injection to the diaphragmatic and topical wash over the liver and gallbladder with bupivacaine or saline. The primary outcome was visual analogue scale pain scores until discharge. Secondary outcomes included pain scores in theatre recovery and analgesic consumption. RESULTS: One patient had a procedure converted to open and was excluded. There was no significant difference in pain scores in the ward or theatre recovery. Analgesic use, respiratory rate, oxygen saturation, duration to ambulation, eating, satisfaction scores, and time to discharge were comparable between the two groups. CONCLUSIONS: IPLA during em-LC does not influence postoperative pain. Other modalities of analgesia should be explored for decreasing the interval between diagnosis of acute admission and em-LC.

关键词： gallbladder stone disease gallbladder cholecystectomy

The NORE1A/RASSF5 Tumor Suppressor Forms a Complex with GSK-3β to Regulate β-Catenin

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journal of Biosciences and Medicines 2024年第8期12卷 60-75页

作者： M. Lee Schmidt Howard Donninger Geoffrey j. Clark Department Pharmacology and Toxicology University of Louisville Louisville USA US Worldmeds LouisvilleUSA Department of Medicine University of Louisville Louisville USA Experimental Therapeutics Program James Graham Brown Cancer Center University of Louisville Louisville USA

NORE1A (RASSF5) is a tumor suppressor of the RASSF family that is often down-regulated in human tumors. NORE1A has multiple roles in controlling cellular homeostasis, one of them being regulating levels of β-catenin by binding and modulating the ubiquitin ligase substrate recognition factor β-TrCP. β-catenin is a major executor of the Wnt pathway. The ubiquitin SCF-β-TrCP ligase complex acts on a phospho-degron site in β-catenin that can be phosphorylated by GSK-3β. We now show that in addition to binding β-TrCP, NORE1A also promotes the phosphorylation of the β-catenin phospho-degron by complexing with the kinase GSK-3β. Indeed, NORE1A enhances the formation of a GSK-3β/β-TrCP complex. A structural mutant of NORE1A that retains β-TrCP binding but will no longer interact with GSK-3β inhibits the β-catenin degrading action of NORE1A. The GSK-3β interaction with NORE1A plays an important role in the biology of NORE1A as a GSK-3β inhibitor blocks NORE1A induced senescence. Thus, we identify a new role for the tumor suppressor NORE1A: The regulation of GSK-3β. GSK-3β has many other substrates including multiple transcription factors and co-activators such as p53 and the Hippo component TAZ. The work implies that NORE1A may be able to influence all of them via this new kinase scaffolding interaction.

关键词： RAS NORE1A RASSF5 GSK-3β Beta Catenin HIPPO