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Release Profile of Lansoprazole Encapsulated in Sodium Alginate Beads

Journal of Pharmacy and Pharmacology 2021年第5期9卷 197-204页

作者： jair pereira de melo junior Vicente Guerra Filho Márcio Rosa JeováDavid Vinicius Cozadi de Souza Vergílio pereira Carvalho department of Biophysics University of Rio VerdeGoiás 75904040Brazil department of Surgery Santa Terezinha HospitalGoiás 75904040Brazil department of Environmental Engineering University of Rio VerdeGoiás 75904040Brazil department of Medicine University of Rio VerdeGoiás 75904040Brazil

Symptomatic gastroesophageal reflux disease (GERD) affects 11% of the population worldwide. Lansoprazole was the second commercially available proton pump inhibitor, recommended for healing and symptomatic relief of reflux esophagitis. This work presents the encapsulation of lansoprazole in sodium alginate beads, generating a controlled release system of the encapsulated drug. The quantification of lansoprazole contents from the encapsulated raw material was carried out, using the methodology prescribed in the Brazilian Pharmacopoeia 5th edition, with adjustments, the encapsulation efficiency and the release profile. Experimental tests were performed using scanning UV-Vis spectrophotometry. Encapsulation efficiency was 100%, obtained from the measurement of the absorbance of the filtrate. Release profile shows the concentration of lansoprazole in the external environment was 4 μg/mL (16%) immediately after 10 min. Concentration of lansoprazole released by the beads follows a linear dynamic as a function of time, with a maximum release equivalent to 25 μg/mL after 210 min (100%). The rate of release is influenced by pH, being higher by 7.4. Beads subjected to heat treatment have a higher release rate at a temperature of 30 °C when compared to a temperature of 60 °C. Increase in temperature decreases the diameter of the beads. The presence of lansoprazole affects the release curve. The absorption rate of the microbeads with lansoprazole is 0.83, and the absorption rate of the microbeads without lansoprazole is 0.58. Sodium alginate beads have a good controlled release effect on lansoprazole.

关键词： Spectrophotometry proton pump inhibitor encapsulation sodium alginate beads.

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Effect of climate and altitude on plant community composition and richness in Brazilian inselbergs

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Journal of Mountain Science 2020年第8期17卷 1931-1941页

作者： Herval Vieira PINTO-junior Pedro Manuel VILLA Luis Fernando Tavares de MENEZES Miriam Cristina Alvarez pereira Universidade Federal de Vigosa Department of Plant BiologyLaboratory of Ecology and Evolution of Plants36570-900Vicosa-MGBrazil Universidade Federal de Vigosa Department of Forest EngineeringLaboratory of Forest Restoration36570-900Vicosa-MGBrazil Universidade Federal do Espirito Santo Department of Agrarian and Biological SciencesLaboratory of Ecology of Restinga and Mata Atlantica29932-900Sao Mateus-ESBrazil Universidade Federal do Espirito Santo Department of Biology29500-000Alegre-ESBrazil

Plant communities are shaped by multiple factors along environmental gradients;however,studies are limited on how environmental filtering drives community composition and species richness on tropical *** evaluate the influence of altitude and climatic variables related to temperature and precipitation on plant community composition and species richness on Brazilian *** assume as a premise that both climate and altitude would induce changes on plant community composition and species richness at the local *** used plant inventory data from 370 sampling units across four inselberg sites in the Atlantic Forest of Espírito Santo State,south-eastern *** tested the univariate and multivariate effects of altitude and climate variables on community composition and species richness with multiple *** in species richness between inselbergs were evaluated using sample-based data to estimate rarefaction and extrapolation *** addition,differences in species composition and taxonomic beta diversity were examined via novel frequency-based metrics.A contrasting climate pattern was observed between the inselberg sites,with south sites being wet compared to the dry conditions found in northern *** richness by rarefaction showed a similar pattern within regional sites;however,there were marked differences between *** richness and beta diversity showed significant differences among sites,with higher values in southern sites than in northern *** a multi-model comparison between inselberg sites,altitude significantly influenced community composition and species richness and explained more variance than climate *** finding suggested that climate could act to some extent on these tropical inselbergs;however,altitude was a better predictor of plant community composition and species richness at the local level.

关键词： Atlantic Forest Biome Beta diversity Abiotic filtering Climate models Environmental filtering Latitudinal gradient

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Are models of volumetric production at the diametric distribution and total stand level mutually compatible?

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Journal of Forestry Research 2020年第5期31卷 1691-1698页

作者： Marcos Felipe de Oliveira Valeriano Eder pereira Miguel Pedro Guilherme de Andrade Vasconcelos Mauro Eloi Nappo Humberto Angelo Alba Valeria Rezende Renan Augusto Miranda Matias Leonardo Job Biali Ilvan Medeiros Lustosa junior department of Forestry Engineering University of Brasilia(UnB)Brası´liaBrazil Federal Institute of Brasılia(IFB) BrasıliaBrazil

The objective of this work was to compare estimates generated by a diametric distribution model and a total stand model against the pre-cut *** model efficiency was also *** were evaluated from 30 permanent sample plots in a Eucalyptus urophylla stand,comprising 24 sample plots used for model fitting,and six sample plots for *** volume of wood per hectare was estimated for different productive units(sites),using 7 years as the reference *** model adjustment quality was verified by adjustment and precision statistics:the correlation between observed and predicted variables,root mean square error percentage,graphical analysis of residual distribution,and a frequency histogram for classes of relative errors and *** the two-parameter Weibull probability density function adhered to the data for tree evolution in diameter classes for the reference age(7 years)in the different productivity classes,it generated imprecise estimates of the number of ***,it produced inaccurate volumetric production *** total stand model provided reliable projections of production volumes in different productivity classes for both adjustment types,showing compatibility with the pre-cut inventory according to a Tukey *** summary,the total stand model generated estimates that were compatible with the pre-cut inventory while the diametric distribution model did not.

关键词： Clutter model Diameter class Eucalyptus Prognosis

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A PCR and RFLP-based molecular diagnostic algorithm for visceral leishmaniasis

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Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 62-70页

作者： Natalia Souza de Godoy Manoel Sebastiao da Costa Lima-junior Jose Angelo Lauletta Lindoso Vera Lucia pereira-Chioccola Thelma Suely Okay Lucia Maria Almeida Braz Laboratorio de Parasitologia Médica Departamento de Moléstias Infecciosas e Parasitárias do Hospital das Clinicas HCFMUSPFaculdade de MedicinaUniversidade de Sao PauloSao PauloBrazil Laboratorio de Imunopatologia e Biologia Molecular-Instituto Aggeu Magalhaes/IAM-FIOCRUZ RecifeBrazil Laboratorio de Soroepidemiologia e Imunobiologia-FMUSP Sao PauloBrazil Laboratorio de Biologia Molecular de Parasitas e Fungos-Instituto Adolfo Lutz Sao PauloBrazil Laboratorio de Parasitologia-Instituto de Medicina Tropical de Sao Paulo-USP Sao PauloBrazil

Objective:To determine an algorithm for molecular diagnosis of visceral leishmaniasis(VL)by kinetoplast DNA(kDNA)(RV1/RV2)and internal transcriber spacer(ITS1)(LITSR/L5.8 S)polymerase chain reaction(PCR),complemented by ITS 1 PCR restriction fragment length polymorphism(RFLP),using peripheral blood or bone marrow aspirate from patients with suspected ***:Biological samples were submitted to the gold standard for the diagnosis of VL and molecular diagnosis represented by ITS 1 PCR,kDNA PCR,and ITS 1 PCR *** samples were obtained from seven groups:groupⅠ,82 samples from patients with confirmed VL;groupⅡ,16 samples from patients under treatment for VL;groupⅢ,14 samples from dogs with canine visceral leishmaniasis(CVL);groupⅣ,a pool of six experimentally infected sandflies(Lutzomya longipalpis);group V,18 samples from patients with confirmed tegumentary leishmaniasis(TL)and groupsⅥandⅦwere from control groups without ***:The following gold standard and molecular examination results were obtained for each of the seven groups:groupⅠ:parasitologic and immunochromatographic tests showed a sensitivity of 76.3%(61 of 80)and 68.8%(55 of 80),respectively,and a sensitivity of 97.6%(80 of 82)and 92.7%(76 of 82)by ITS1 and kDNA PCR,*** ITS1 PCR RFLP(HaeⅢ)analysis of the 80 positive samples,52.5%(42 of 80)generated three fragments of 180,70,and 50 bp,corresponding to the pattern of Leishmania infantum infantum;groupⅡ:negative for the parasitologic methods and positive for IrK39(100%,16 of 16),presented 12.5%(2 of 16)of positivity by ITS 1 PCR and 25.0%(4 of 16)by kDNA PCR;groupⅢ:positive in the parasitologic and serologic tests(100%,14 of 14),presented 85.7%(12 of 14)of positivity by ITS1 PCR and kDNA ***1 PCR RFLP showed that 83.3%(10 of 12)of the canine samples contained parasites with profiles similar to ***;groupⅣpresented amplifications by ITS1 PCR and kDNA ***1 PCR products were analyzed by RFLP,generating a profile similar to

关键词： Leishmania infantum Molecular diagnosis Visceral leishmaniasis PCR RFLP

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Evaluation of antibacterial and toxicological activities of essential oil of Ocimum gratissimum *** its major constituent eugenol

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Food Bioscience 2022年第6期50卷 2152-2159页

作者： Julio Cesar Silva Raimundo Luiz Silva pereira Thiago Sampaio de Freitas Janaína Esmeraldo Rocha Nair Silva Macedo Carla de Fatima Alves Nonato Marina Leite Linhares Daniely Sampaio Arruda Tavares Francisco Assis Bezerra da Cunha Henrique Douglas melo Coutinho Sidney Gonçalo de Lima Francisco Nascimento pereira-junior Francisco Paulo Araújo Maia Ivo Cavalcante Pita Neto Fabiola Fernandes Galvão Rodrigues George Joaquim Garcia Santos department of Biological Chemistry Regional University of CaririR.Cel.Antonio Luis 116163105000CratoCEBrazil University Center Doutor Leão Sampaio Av.Leão Sampaio 40063040000Juazeiro do NorteCEBrazil Research and development Center for Medicines Federal University of CearaR.Coronel Nunes de Melo 100060430275FortalezaCEBrazil Chemistry department Federal University of PiauiCampus Universitário Ministro Petrônio Portella64049550TeresinaPIBrazil Center for Agricultural and Biodiversity Sciences - CCAB Federal University of CaririCratoCearáBrazil CECAPE - College of dentistry Juazeiro do NorteCE63024-015Brazil

The present study aimed to comparatively analyze the antibacterial activity,combined with antibiotics,and toxicological activity of the essential oil of *** *** its major compound,*** phytochemical analysis of the essential oil was performed by gas chromatography coupled with mass *** minimum inhibitory concentration and combined antibiotic activity were performed by *** melanogaster was the model organism chosen to evaluate the toxicological response of *** chromatographic assay showed eugenol as the major compound of the essential oil of *** *** microbiological assays showed that in terms of antibacterial activity,both products showed similar activity,quite significant in Gram-negatives,such as those of strains of Pseudomonas *** results were verified in the modulatory activity,allowing the verification that the bioactivity performed by the essential oil of *** *** mainly influenced by its major *** the EC50 of the compounds varied subtly,the essential oil was less toxic compared to *** information obtained in the present study demonstrates that the essential oil of *** *** a promising antibacterial agent and better tolerated than its majority compound alone,in the model organism ***.

关键词： Antibacterial agents Drosophila melanogaster Eugenol Ocimum gratisismumum L.essential oils

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High-throughput phenotyping of two plant-size traits of Eucalyptus species using neural networks

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Journal of Forestry Research 2022年第2期33卷 591-599页

作者： Marcus Vinicius Vieira Borges Janielle de Oliveira Garcia Tays Silva Batista Alexsandra Nogueira Martins Silva Fabio Henrique Rojo Baio Carlos Antônio da Silva junior Gileno Brito de Azevedo Glauce Taís de Oliveira Sousa Azevedo Larissa pereira Ribeiro Teodoro Paulo Eduardo Teodoro Federal University of Mato Grosso Do Sul(UFMS) Chapadão Do SulMato Grosso Do Sul 79560000Brazil department of Geography State University of Mato Grosso(UNEMAT)SinopMato Grosso 78555000Brazil

In forest modeling to estimate the volume of wood,artificial intelligence has been shown to be quite effi-cient,especially using artificial neural networks(ANNs).Here we tested whether diameter at breast height(DBH)and the total plant height(Ht)of eucalyptus can be pre-dicted at the stand level using spectral bands measured by an unmanned aerial vehicle(UAV)multispectral sensor and vegetation *** do so,using the data obtained by the UAV as input variables,we tested different configurations(number of hidden layers and number of neurons in each layer)of ANNs for predicting DBH and Ht at stand level for different Eucalyptus *** experimental design was randomized blocks with four replicates,with 20 trees in each experimental *** treatments comprised five Eucalyptus species(***,***,***,***-dis,and ***)and Corymbria *** and Ht for each plot at the stand level were measured seven times in separate overflights by the UAV,so that the multispectral sensor could obtain spectral bands to calculate vegetation indices(VIs).ANNs were then constructed using spectral bands and VIs as input layers,in addition to the categorical variable (species), to predict DBH and Ht at the stand level simultaneously. This report represents one of the first appli-cations of high-throughput phenotyping for plant size traits in Eucalyptus species. In general, ANNs containing three hidden layers gave better statistical performance (higher esti-mated r, lower estimated root mean squared error-RMSE) due to their greater capacity for self-learning. Among these ANNs, the best contained eight neurons in the first layer, seven in the second, and five in the third (8 − 7 − 5). The results reported here reveal the potential of using the gener-ated models to perform accurate forest inventories based on spectral bands and VIs obtained with a UAV multispectral sensor and ANNs, reducing labor and time.

关键词： Computational intelligence Diameter at breast height Forest inventory Remote sensing Vegetation indices

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Impacts of COVID-19 on children and adolescents:A systematic review analyzing its psychiatric effects

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World Journal of Psychiatry 2022年第11期12卷 1313-1322页

作者： Italo Wanderson de Moura Gabriel Danielly Gonçalves Sombra Lima Jeully pereira Pires Nélio Barreto Vieira Aloisio Antonio Gomes de Matos Brasil Yara Talita Gomes pereira Erika Galvao de Oliveira HildsonLeandro de Menezes Nadia Nara Rolim Lima Alberto Olavo Advíncula Reis Ruan Neto pereira Alves Uanderson pereira da Silva Jucier Gonçalves junior Modesto Leite Rolim-Neto School of Medicine Faculty of Medicine of Juazeiro do Norte-FMJ/IDOMEDJuazeiro do Norte 63040-360CearaBrazil School of Medicine Federal University of CaririBarbalha 63048-080CearaBrazil Mais Médicos Program Federal Government of Brazil-Ministry of HealthIguatu 63048-080CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital HALHMBelo Jardim 55157-290PernambucoBrazil Nursing department Doctor Leão Sampaio University Center-UNILEAOJuazeiro do Norte 63.041-140CearaBrazil Julio Alves de Lira Hospital and Maternity Hospital JALHMBelo Jardim 55157-290PernambucoBrazil Graduate Program(Post-Doctoral)in Neuro-Psychiatry UFPERecife 50670-901PernambucoBrazil Postgraduate Program(Master’s and Doctorate)in Public Health University of São Paulo-USPSão Paulo 01246-904São PauloBrazil School of Education USPSão Paulo 05508-040São PauloBrazil Internal Medicine-Division of Rheumathology at Hospital das Clínicas São Paulo UniversitySão Paulo 01246-903São PauloBrazil

OBJECTIVE To summarize the most relevant data from a systematic review on the impact of COVID-19 on children and adolescents,particularly analyzing its psychiatric *** This review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses(PRISMA)guidelines and included experimental studies(randomized-individually or pooled-and non-randomized controlled trials),observational studies with a group for internal comparison(cohort studies-prospective and retrospective-and case-control)and qualitative studies in the period from 2021 to *** The search identified 325 articles;we removed 125 *** selected 200 manuscripts,chosen by title and selected *** excluded 50 records after screening titles and abstracts,as they did not meet the inclusion *** retrieved 150 records selected for a full *** excluded 90 text articles and we selected 25 records for the(n)***:Due to the short period of data collection,from 2021 to 2022,there is a possibility of lack of relevant studies related to the mental health care of children and *** addition,there is the possibility of publication bias,such as only significant findings being *** The impact of COVID-19 on the mental health of children and adolescents is of great concern to child and youth *** such as fear,anxiety,panic,depression,sleep and appetite disorders,as well as impairment in social interactions caused by psychic stress,are punctual markers of pain and psychic suffering,which have increasing impacts on the mental health panorama of children and adolescents globally,particularly in vulnerable and socially at-risk populations.

关键词： Child psychiatry Adolescent psychiatry Mental health COVID-19 Kids Teens

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Prognosis of forest production using machine learning techniques

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Information Processing in Agriculture 2023年第1期10卷 71-84页

作者： Jeferson pereira Martins Silva Mayra Luiza Marques da Silva Adriano Ribeiro de Mendonca Gilson Fernandes da Silva Antoˆnio Almeida de Barros junior Evandro Ferreira da Silva Marcelo Otone Aguiar Jeangelis Silva Santos Nı´vea Maria Mafra Rodrigues Federal University of Espirito Santo UFESDepartment of Forestry and Wood ScienceAvenue Governor Lindemberg31629550-000 Jeroˆnimo MonteiroESBrasil b Departamento de Cieˆncias Agra´riasUniversidade Federal de Sa o Joa del-Rei Sete Lagoas CampusHighway MG 424-Km 47Sete LagoasMinas Gerais 35701-970Brazil Federal University of Para(UFPA) University Campus of AltamiraStreet Cel.Jose Porfırio2515Sa o Sebastiao68372-040 AltamiraPABrazil

Forest production and growth are obtained from statistical models that allow the generation of information at the tree or forest stand *** the use of regression models is common in forest measurement,there is a constant search for estimation procedures that provide greater ***,machine learning techniques have been used with satisfactory performance in measuring ***,methods such as Adaptive NeuroFuzzy Inference System(ANFIS)and Random Forest are relatively poorly studied for predicting the volume of wood in eucalyptus plantations in ***,it is essential to check whether these techniques can provide gains in terms of ***,this study aimed to evaluate the use of Random Forest and ANFIS techniques in the prognosis of forest *** data used come from continuous forest inventories carried out in stands of eucalyptus *** data were divided into 70%for training and 30%for *** algorithms used to generate rules in ANFIS were Subtractive Clustering and ***,training was done with the hybrid algorithm(descending gradient and least squares)with the number of seasons ranging from 1 to *** RFs were trained,varying the number of trees from 50 to 850 and the number of observations by five leaves to *** neural networks and decision trees were also trained to compare the feasibility of the *** evaluation of the estimates generated by the techniques for training and validation was calculated based on the following statistics:correlation coefficient(r),relative Bias(RB),and the relative root mean square error(RRMSE)in *** general,the techniques studied in this work showed excellent performance for the training and validation data set with RRMSE values*** RF presented inferior statistics about the ANFIS for the prognosis of forest *** Subtractive Clustering(SC)and Fuzzy-C-Means(FCM)algorithms provide accurate baseline

关键词： Artificial Intelligence Ensemble Learning Forest Measurement Forest inventory Artificial Neural Networks Regression

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Microbial enzymatic stoichiometry and the acquisition of C, N,and P in soils under different land-use types in Braziliansemiarid

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Soil Ecology Letters 2023年第3期5卷 101-108页

作者： Erika Valente de Medeiros Érica de Oliveira Silva Gustavo pereira Duda Mario Andrade Lira junior Uemeson Josédos Santos Claude Hammecker Diogo Paes da Costa Fabio Fernando Araujo Arthur Prudêncio de Araujo pereira Lucas William Mendes Ademir Sergio Ferreira Araujo Laboratory of Microbiology and Enzymology-LEMA Federal University of Agreste PernambucoGaranhuns 55292-270Brazil Federal Rural University of Pernambuco Rua Dom Manuel de Medeiross/nDois Irmãos52171-900RecifeBrazil Soil-Agrosystem-Hydrosystem Interaction Lab-LISAH Place Pierre Viala234060 MontpellierFrance Universidade do Oeste Paulista Presidente PrudenteSPBrazil departamento de Ciência Do Solo Universidade Federal Do CearáFortalezaCEBrazil Center for Nuclear Energy in Agriculture University of Sao PauloPiracicabaSPBrazil Soil Quality Lab Agricultural Science CenterFederal University of PiauíTeresinaPI 60049-550Brazil

This study hypothesized that different land-use affect the microbial enzymatic stoichiometry and C-,N-,and P-acquisition in Brazilian semiarid ***,the enzymesβ-glucosidase(C-acquiring enzyme),urease(N-acquiring enzyme),and acid phosphatase(P-acquiring enzyme)were assessed in soil samples collected at 0−5 and 5−10 cm depth from a tropical dry forest,a protected area with Angico,a protected area with Ipê,scrub area,and an agricultural area with *** values of C-,N-,and P-acquiring enzymes were used to calculate the enzymatic C:N,C:P,and N:P *** values of C:P and N:P ratios were higher at 0−5 cm depth,while no significant variation,between soil depth,was observed for C:N *** values of C-and N-acquiring enzymes were higher at 0−5 cm in tropical dry forest areas and Angico forest,*** all land use types,the values of vectors L and A were higher than 1°and 45°,*** study showed that both land-use and soil depth influence the enzymatic stoichiometry,showing higher values of C-and N-acquiring enzymes in native and protected forests at soil surface.

关键词： C:N:P stoichiometry tropical forests soil depth Luvisols

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The Spreading depression Propagation: How Electrochemical Patterns Distort or Create Perception

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Open Journal of Biophysics 2021年第2期11卷 133-146页

作者： Vera Maura Fernandes de Lima Alfredo pereira junior Guilherme Lima de Oliveira Centro de Biotecnologia CNEN/IPEN-SP S&atilde o Paulo Brazil department of Human and Nutritional Sciences Biosciences Inst. UNESP Botucatu S&atilde o Paulo Brazil S&atilde o Paulo S&atilde o Paulo Brazil

At the transition from quiescence to propagating waves recorded in isolated retinas, a circular electric current closes in the extracellular matrix;this circular current creates a magnetic torus flow that, when entering quiescent tissue in front of the wave, recruits elements and when leaving behind, helps to build the absolute refractory state. The waving magnetic torus is the consequence of the vortex effect and explains the energy boost that drives propagation. Methods: We interpret experimental results from intrinsic and extrinsic fluorescence dyes, voltage, calcium and pH sensitive, optical signals from isolated retinas, and time series recordings using ion exchange resins: Ca, K, pH, Na, Cl recorded extracellularly at retinas, cerebellums and cortices coupled to spreading depression waves. Finally, we checked the ECoG activity, also a time series, at the transition from after discharges to spreading depression in rat hippocampus. Results: The integrated assessment of the diversified measurements led to the realization that the magnetic flow at the wavefront is a major contributor to the wave propagation mechanisms. This flow couples mass and charge flows as a swirling torus from excited to quiescent tissue. Conclusions: An alternative model of the brain is possible, apart from the classical HH and molecular biology model. Physical chemistry of charged gels and its flows explains the results. The conceptual framework uses far from equilibrium thermodynamics.

关键词： Brain Electrochemistry Retinal Model Resonance Spreading depression Functional CNS Syndromes Vortex Effect

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