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Open Journal of Stomatology 2018年第6期8卷 189-195页

作者： Minor Enrique Zuniga Araya Rita Elizabeth Martínez Martínez Jose Honorio Olvera Delgado jaime alejandro nieto ramirez Elena A.Popoca Hernandez Jose Luis Ayala Herrera San Luis Potosi University San Luis PotosiMexico Universidad Autónoma de San Luis Potosí San Luis PotosíMéxico

Background: The collapse of the alveolar ridge in an edentulous area in patients who will undergo oral rehabilitation impedes the harmonic relationship between pontic and ridge. To correct this type of defect there are several surgical techniques that aim to achieve soft tissue augmentation, allowing the conformation of an ideal profile with the use of a pontic. The aim was to demonstrate how the technique of tissue modification in an area with a class b defect of Allen allows the correction of aesthetics in the anterior dental sector. Case presentation: A 41-year-old Mexican woman. Extra and intraoral examination was performed, the dental treatment included connective tissue graft and rehabilitation with Zirconia. Conclusion: Surgery of residual alveolar ridge augmentation with connective tissue graft gives the patient favorable aesthetic results in the anterior area when it will be rehabilitated.

关键词： Esthetic Zone Zirconia Class B Defect of Allen

Molecular and in vitro Characterization of Field Isolates of Bovine Herpesvirus-1

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Virologica Sinica 2012年第1期27卷 26-37页

作者： Julian Ruiz-Saenz Jairo jaime Gloria ramirez Victor Vera Faculty of Veterinary Medicine and Animal Science National University of Colombia

Bovine Herpesvirus-1 (BoHV-1) is distributed worldwide and is a major pathogen in cattle, being the causal agent of a variety of clinical syndromes. The aim of this study was to isolate and to characterize (molecular and biological characterization) BoHV-1 from 29 immunosuppressed animals. It was possible to obtain 18 isolates, each from a different animal, such as from the respiratory and reproductive tracts. In some cases the cytopathic effect was visible 12 hours post-inoculation, and became characteristic after 36-48 hours. Biological characteristics were evaluated and compared with Iowa and Colorado-1 reference strains, and differences were found in plaque size, virus titer measured by TCID50 and PFU/mL, and one step virus curves. These results showed that some isolates had a highly virulent-like behavior in vitro, compared to the reference strains, with shorter eclipse periods, faster release of virus into the supernatants, and higher burst size and viral titer. There were no differences in glycoprotein expression of BoHV-1 isolates, measured by Western blot on monolayers. Moreover, using restriction endonucleases analysis, most of the viruses were confirmed as BoHV-1.1 and just one of them was confirmed as BoHV-1.2a subtype. These findings suggest that some wild-type BoHV-1 isolates could be useful as seeds to develop new monovalent vaccines.

关键词： Bovine herpesvirus 1 Genital infection Respiratory infection Latent infection

A look at the ASEAN-NDI: building a regional health R&D innovation network

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Infectious Diseases of Poverty 2014年第1期3卷 135-144页

作者： jaime C Montoya Carina L Rebulanan Nico Angelo C Parungao Bernadette ramirez Department of Science and Technology-Philippine Council for Health Research and Development(DOST-PCHRD) Taguig CityPhilippines UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases(TDR) World Health OrganizationGenevaSwitzerland

Globally,there are growing efforts to address diseases through the advancement in health research and development(R&D),strengthening of regional cooperation in science and technology(particularly on product discovery and development),and implementation of the World Health Assembly Resolution 61.21(WHA61.21)on the Global Strategy and Plan of Action on Public Health,Innovation,and Intellectual Property(GSPA-PHI).As such,the Association of Southeast Asian Nations(ASEAN)is responding to this through the establishment of the ASEAN-Network for Drugs,Diagnostics,Vaccines,and Traditional Medicines Innovation(ASEAN-NDI).This is important in the ASEAN considering that infectious tropical diseases remain prevalent,emerging,and reemerging in the *** paper looks into the evolution of the ASEAN-NDI from its inception in 2009,to how it is at present,and its plans to mitigate public health problems regionally and even globally.

关键词： ASEAN-NDI Health research and development Infectious tropical diseases Innovation network Strategic business plan

Association of swine influenza H1N1 pandemic virus(SIV-H1N1p) with porcine respiratory disease complex in sows from commercial pig farms in Colombia

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Virologica Sinica 2014年第4期29卷 242-249页

作者： Luisa Fernanda Mancipe Jiménez Gloria Ramírez nieto Victor Vera Alfonso Jairo jaime Correa Laboratory of Animal Virology Faculty of Veterinary Medicine and Animal Science National University of Colombia

Porcine respiratory disease complex （PRDC） is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus （PRRSV）, swine influenza virus （SIV）, Mycoplasma hyopneumoniae （Myh）, Actinobacillus pleuropneumoniae （APP）, Pasteurella multocida and Porcine circovirus 2 （PCV2）. To characterize the specific role of swine influenza virus in PRDC presentation in Colombia, 11 farms from three major production regions in Colombia were examined in this study. Nasal swabs, bronchial lavage and lung tissue samples were obtained from animals displaying symptoms compatible with SIV. Isolation of SIV was performed in 9-day embryonated chicken eggs or Madin-Darby Canine Kidney （MDCK） cells. Positive isolates, identified via the hemagglutination inhibition test, were further analyzed using PCR. Overall, 7 of the 11 farms were positive for SIV. Notably, sequencing of the gene encoding the hemagglutinin （HA） protein led to grouping of strains into circulating viruses identified during the human outbreak of 2009, classified as pandemic H1N1-2009. Serum samples from 198 gilts and multiparous sows between 2008 and 2009 were obtained to determine antibody presence of APP, Myh, PCV2 and PRRSV in both SIV-H1Nlp-negative and -positive farms, but higher levels were recorded for SIV- HI Nlp-positive farms. Odds ratio （OR） and P values revealed statistically significant differences （p〈0.05） in PRDC presentation in gilts and multiparous sows of farms positive for SIV-HINlp. Our findings indicate that positive farms have increased risk of PRDC presentation, in particular, PCV2, APP and Myh.

关键词： SIV-HIN1 swine influenza pandemic porcine respiratory disease complex Colombia

First isolation and identification of H1N1 swine influenza viruses in Colombian pig farms

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Health 2012年第10期4卷 983-990页

作者： Gloria Consuelo ramirez-nieto Cesar Augusto Diaz Rojas Victor Julio Vera Alfonso Jairo jaime Correa Jose Dario Mogollon Galvis Línea de Microbiología y Epidemiología Posgrado en Salud Animal Facultad de Medicina Veterinaria y de Zootecnia Universidad Nacional de Colombia Bogotá Colombia

The pig industry in Colombia has grown 30% in the last decade achieving high levels of technology and efficiency;in spite of that, respiratory diseases remain a constraint. Since 1970, serological evidence and histological findings suggested the role of swine influenza virus (SIV) as part of the porcine respiratory disease complex;nevertheless, elusive and molecular typing isolates are missing. This study was aimed at isolating SIV from intensive pig farms and to achieve molecular characterization to determine strains circulating in the field. In order to accomplish this goal, 242 samples were taken from nasal swabs, 25 from bronchial washes and 8 from lung tissue. Samples were collected during a period of three years, between 2008 and 2010 and were originated from 78 farms of the three main pig production regions of the country. The samples were transported in BHI broth with 2% antibiotic and antimycotic solution and stored at –70?C until processed. The swabs were inoculated in 9 - 11 days old embryo chicken eggs and in MDCK (Madin Darby Canine Kidney) cell cultures with the addition of trypsin. The isolates were identified by the HA (hemoagglutination) test and by RT-PCR targeting the HA (hemagglutinin), NA (Neuraminidase) and M (Matrix) genes. Full length sequence of the HA and NA glycoproteins from four selected virus isolates was conducted (Macrogen?. USA). As a result, fifteen SIV isolates from nine farms distributed in the three regions were obtained. Twelve of the isolates are related to the swine origin H1N1 virus that caused the 2009 influenza pandemic. The remaining three viruses were related to classical swine influenza viruses.

关键词： Swine Influenza H1N1 Colombia First Isolation Classical Pandemic

Hepatocellular carcinoma and hepatitis C virus infection in Latin America: epidemiology, diagnosis and treatment

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Hepatoma Research 2020年第5期6卷 1-18页

作者： Marina Galicia-Moreno Hugo Christian Monroy-ramirez Marina Campos-Valdez jaime Sanchez-Meza Laura Sanchez-Orozco Juan Armendariz-Borunda University of Guadalajara Institute of Molecular Biology in Medicine and Gene TherapyDepartment of Molecular Biology and GenomicsHealth Science University Center(CUCS)Jalisco 44340Mexico Tecnologico de Monterrey Campus GuadalajaraJalisco 45138Mexico

Hepatocellular carcinoma(HCC)is the most common cancer associated with chronic liver disease and *** most common cause of HCC is chronic hepatitis C virus infection and many studies in Europe,Asia and North America have focused on its etiology,epidemiology,diagnostic tools,and therapeutic ***,little is known about these issues in Latin *** aim of this review is to address these aspects of HCC in Latin *** main risk factors associated with developing HCC in this region are:age,concomitant cirrhosis,hepatitis C infection,obesity and hereditary disease such as *** the other hand,screening tests and diagnostic methods of HCC are mostly serum alpha fetoprotein quantification,liver ultrasound,computed tomography,magnetic resonance,and *** diagnostic methods include gut microbiota analysis and the use of nanotechnology and they continue to be ***,according to the Barcelona Clinic Liver Cancer,curative treatments used in HCC patients are mainly liver resection,liver transplantation,and local ablation,each with advantages and *** conclusion,clear strategies are urgently needed to understand the extent of HCC and related problems in this part of the *** review provides greater knowledge of HCC for the proper design of preventive programs by taking into consideration specific characteristics of our ***,this review allows for an understanding of individualizing treatments according to the patient’s needs.

关键词： Liver hepatitis C epidemiology diagnosis treatment hepatocellular carcinoma Latin America

Kinetics of the H＋-ATPase from Dry and 5-Hours- Imbibed Maize Embryos in Its Native, Solubilized, and Reconstituted Forms

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Molecular Plant 2011年第3期4卷 505-515页

作者： Sobeida Sanchez-nieto Consuelo Enriquez-Arredondo Fernando Guzmbn-Chavez Rolando Hern&ndez-Munoz Jorge ramirez Marina Gavilanes-Ruiz Departamento de Bioquimica Facultad de Quimica Conjunto E. Universidad Nacional Autonoma de Mexico Cd Universitaria 04360 Mexico DF Mexico Instituto de Fisiologfa Celular Universidad Nacional Autonoma de Mexico Cd Universitaria 04360 Mexico DF Mexico

Membranes undergo recovery upon rehydration in seed germination. Previous work has described that the plasma membrane H＋-ATPase from maize embryos adopts two different forms at 0 and 5 h of imbibition. We investigated how the kinetics of these two forms could be affected by alterations in the plasma membrane （PM）. In comparison to the O-h, PMs from the 5-h imbibed embryos showed changes in glycerophospholipid composition, decrease in leakage, and increase in fluidity. Kinetics of the PM H^-ATPase from 0 and 5-h imbibed embryos showed negative cooperativity, With the removal of the membrane environment, the activity of the enzymes shifted to a more complex kinetics, displaying two enzyme components. Lipid reconstitution produced one component with positive cooperativity. In all cases, enzymes from 0 and 5-h imbibed embryos presented similar kinetics with some quantitative differences. These results indicate that the two enzyme forms have the potential ability to respond to changes in the membrane enyiror^rpent, but the fact that they do not show differences in the native membranes at 0 or 5 h implies that modifications in the membrane are not drastic enough to alter their kinetics, or that they are able to preserve their boundary lipids or associated proteins and thus retain the same kinetic behavior.

关键词： ATPase activity H^-ATPase maize germination plasma membrane H+-ATPase plasma membrane Zea mays.

Chemical Modification of Halloysite Nanotubes for the Preparation of Nanocomposites on Non Polar Matrix

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Journal of Mechanics Engineering and Automation 2019年第4期9卷 128-132页

作者： Alan ramirez Guevara jaime Bonilla Ríos Laura Eugenia Romero Robles Department of Chemistry and Nanotechnology Monterrey Institute of Technology and Higher EducationMonterrey 64849N.L.México

In this study,we demonstrated the effects of chemical treatments for Halloysite nanotubes(HNTs)under acid and alkaline conditions using sulfuric acid and sodium *** results indicate that alkaline treatment destroyed the crystalline structure and morphology for HNTs because the XRD spectrum shows the typical peaks for *** the acid treatment using H2SO4,XRD spectrum indicates an intensity reduction for the peak(001)showing a lower concentration of aluminium in the *** reflectance analysis shows a reduction of 40 and 15%for reflectance with H2SO4 and NaOH treatments respectively.A terephthalic acid adsorption test was realized with the HNTs,modified halloysites(HNT-H2SO4)and(HNT-NaOH)samples with a kinetic study and it was quantified with UV spectroscopy at 240 nm where results shown a lower adsorption for HNTs treated with H2SO4 in comparison with alkaline treatment and not treated HNT.A decrease of 58%±0.3was achieved with the sulfuric acid treatment with not crystalline structure modification using ICP technique to quantify the sample compositions.

关键词： Halloysite nanotubes acid/basic treatment aluminium sulfate physical adsorption Fourier-transform infraredspectroscopy (FTIR) XRD diffuse transmittance

Air Quality Regarding to TSP in Six Cities of Sonora,Mexico,a Criticism to the NOM-025-SSA1-1993 and a Proposed Criterion for Its Non-Compliance

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Journal of Environmental Protection 2014年第10期5卷 862-873页

作者： Martin Eusebio Cruz-Campas Agustin Gomez-Alvarez Margarito Quintero-Nunez Roberto ramirez-Leal jaime Varela-Salazar Onofre Monge-Amaya Engineering Institute Autonomous University of Baja CaliforniaMexicaliMexico Environmental Industrial Engineering Program Sonora State UniversityHermosilloMexico Chemical Engineering and Metallurgy Department University of SonoraHermosilloMexico

In the present study, the air quality is assessed for the year 2010 regarding to the Total Suspended Particles (TSP) for six cities of Sonora, Mexico, representing the first regional study in Sonora in air quality. The assessment used performance indicators and indicators of compliance with the regulations. It is established that in all the cities the maximum limit value of daily concentration of 210 μg/m3 is exceeded, being the percentage of days above the rule of 30%, 78%, 76%, 6%, 3% and 62% for Agua Prieta, Nogales, Puerto Penasco, Hermosillo, Guaymas and Obregón respectively, classifying these days with poor air quality. According to the annualized index used, the air quality was not satisfactory for the period of study in the six cities. Nogales and Puerto Penasco presented the most adverse conditions of air quality with annual average values of TSP of 363 and 345 μg/m3 and maximum daily of 1047 and 1239 μg/m3 (498% and 590% above the norm) respectively. The requirements of coverage that establishes the Mexican Official Standard NOM-025-SSA1-1993 (SSA, 2005) are questioned for its compliance, proposing in this paper a criterion of non-compliance by prioritizing the protection of health and the precautionary principle. It is recommended to implement air quality management programs (PROAIRE) in these cities.

关键词： Particles TSP Air Quality NOM-025-SSA1-1993 Sonora Mexico

Fostering social innovation and building adaptive capacity for dengue control in Cambodia: a case study

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Infectious Diseases of Poverty 2020年第5期9卷 93-104页

作者： Pierre Echaubard Chea Thy Soun Sokha Set Srun Claudia nieto-Sanchez Koen Peters Grietens Noel R.Juban Jana Mier-Alpano Sucelle Deacosta Mojgan Sami Leo Braack Bernadette ramirez Jeffrey Hii SOAS University London Thornhaugh StreetLondon WC1H 0XGUK Malaria Consortium Phnom PenhCambodia Institute of Tropical Medicine AntwerpBelgium Social Innovation and Health Initiatives University of the PhilipinesManillaPhilippines California State University FullertonUSA UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases GenevaSwitzerland Australian Institute of Tropical Health&Medicine James Cook University of North QueeslandTownsvilleQLDAustralia

Background:The social-ecological systems theory,with its unique conception of resilience(social-ecological systems&resilience,SESR),provides an operational framework that currently best meets the need for integration and adaptive governance as encouraged by the Sustainable Development *** accounts for the complex dynamics of social-ecological systems and operationalizes transdisciplinarity by focusing on community engagement,value co-creation,decentralized leadership and social *** Social Innovation(SI)in the context of implementation research for vector-borne diseases(VBD)control offers a low-cost strategy to contribute to lasting and contextualized community engagement in disease control and health development in low and middle income countries of the global *** this article we describe the processes of community engagement and transdisciplinary collaboration underpinning community-based dengue management in rural primary schools and households in two districts in ***:Multiple student-led and community-based interventions have been implemented focusing on empowering education,communication for behavioral change and participatory epidemiology mapping in order to engage Cambodian communities in dengue *** describe in particular the significance of the participatory processes that have contributed to the design of SI products that emerged following iterative consultations with community stakeholders to address the dengue ***:The SI products that emerged following our interaction with community members are 1)adult mosquito traps made locally from solid waste collections,2)revised dengue curriculum with hands-on activities for transformative learning,3)guppy distribution systems led by community members,4)co-design of dengue prevention communication material by students and community members,5)community ***:The initiative described in this article put in motion processes of community engagemen

关键词： Social-ecological system Community engagement Transdisciplinarity Health development Sustainability Integrated vector management Social innovation in health