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Aberrant promoter methylation of p16 in colorectal adenocarcinoma in North Indian patients

World Journal of Gastrointestinal Oncology 2010年第7期2卷 295-303页

作者： Pooja Malhotra Rakesh Kochhar Kim Vaiphei jai dev wig Safrun Mahmood Department of Gastroenterology Postgraduate Institute of Medical Education and Research Department of Histopathology Postgraduate Institute of Medical Education and Research Department of General Surgery Postgraduate Institute of Medical Education and Research Department of Experimental Medicine and Biotechnology Postgraduate Institute of Medical Education and Research

AIM:To investigate p16 gene methylation and its expression in 30 patients with sporadic colorectal adenocarcinoma in a North Indian population. METHODS:Methylation specific polymerase chain reaction was used to detect p16 gene methylation and immunohistochemistry was used to study the p16 expression in 30 sporadic colorectal tumors as well as adjoining and normal tissue ***:Aberrant promoter methylation of p16 gene was detected in 12(40%)tumor specimens,whereas no promoter methylation was observed in adjoining and normal *** showed expression of p16 protein in 26(86.6%)colorectal tumors whereas complete loss of expression was seen in 4(13.3%)and reduced expression was observed in 12(40%)tumors. In the adjoining mucosa,expression of p16 was in 11 (36.6%)whereas no clear positivity for p16 protein was seen in normal *** was a significant difference in the expression of p16 protein in tumor tissue and adjoining mucosa(P0.05). CONCLUSION:Our study demonstrated that promoter hypermethylation of the p16 gene results in loss/reduced expression of p16 protein and this loss/reduced expression may contribute to tumor enlargement.

关键词： Methylation specific polymerase chain reaction p16 Methylation Immunohistochemistry Colorectal cancer

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