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Dynamic changes of protein corona compositions on the surface of zinc oxide nanoparticle in cell culture media

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Frontiers of Chemical Science and Engineering 2019年第1期13卷 90-97页

作者： Vo-Van Giau Yoon-Hee Park Kyu-Hwan shim Sang-Wook Son Seong-Soo A. An Department of Bionanotechnology and Gachon Medical Research Institute Gachon University and Gachon Medical Research Institute Seongnam Korea Laboratory of Cell Signaling and Nanomedicine Department of Dermatology and Division of Brain Korea 21 Project for Biomedical Science Korea University College of Medicine Seoul Korea

The potential applications of nanomaterials used in nanomedicine as ingredients in drug delivery systems and in other products continue to expand. When nanomaterials are introduced into physiological environments and driven by energetics, they readily associate proteins forming a protein corona (PC) on their surface. This PC could result in an alteration of the nanomatcrial's surface characteristics, affecting their interaction with cells due to conformational changes in adsorbed protein molecules. However, our current understanding of nanobiological interactions is still very limited. Utilizing a liquid chromatography-mass spectroscopy/mass spectroscopy technology and a Cytoscape plugin (ClueGO) approach, we examined the composition of the PC for a set of zinc oxide nanoparticles (ZnONP) from cell culture media typically and further analyzed the biological interaction of identified proteins, respectively. In total, 36 and 33 common proteins were investigated as being bound to ZnONP at 5 min and 60 min, respectively. These proteins were further analyzed with ClueGO, a Cytoscape plugin, which provided gene ontology and the biological interaction processes of identified proteins. Proteins bound to the surface of nanoparticles that may modify the structure, therefore the function of the adsorbed protein could be consequently affect the complicated biological processes.

关键词： ZnONPs nanoparticles protein corona ClueGO

Multiple functions of SWI/SNF chromatin remodeling complex in plant-pathogen interactions

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Stress Biology 2021年第1期1卷 215-229页

作者： Yunqing Jian Won-Bo shim Zhonghua Ma State Key Laboratory of Rice Biology and Key Laboratory of Molecular Biology of Crop Pathogens and InsectsInstitute of BiotechnologyZhejiang UniversityHangzhouChina Department of Plant Pathology and Microbiology Texas A&M UniversityCollege StationTXUSA

The SWI/SNF chromatin remodeling complex utilizes the energy of ATP hydrolysis to facilitate chromatin access and plays essential roles in DNA-based *** in animals,plants and fungi have uncovered sophisticated regulatory mechanisms of this complex that govern development and various stress *** this review,we summarize the composition of SWI/SNF complex in eukaryotes and discuss multiple functions of the SWI/SNF complex in regulating gene transcription,mRNA splicing,and DNA damage *** review further highlights the importance of SWI/SNF complex in regulating plant immunity responses and fungal ***,the potentials in exploiting chromatin remodeling for management of crop disease are presented.

关键词： Chromatin remodeling SWI/SNF complex Transcription Splicing DNA damage repair Plant-pathogen interaction

Peritonitis with small bowel perforation caused by a fish bone in a healthy patient

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World Journal of Gastroenterology 2014年第6期20卷 1626-1629页

作者： Yonghoon Choi Gyuwon Kim Chansup shim Dongkeun Kim Dongju Kim Department of Internal Medicine Sahmyook Seoul Hospital Department of Gastroenterology Sahmyook Seoul Hospital Digestive Disease Center Konkuk University Medical Center

Perforation of the gastrointestinal tract by ingested foreign bodies is extremely rare in otherwise healthy patients, accounting for < 1% of cases. Accidentally ingested foreign bodies could cause small bowel perforation through a hernia sac, Meckel’s diverticulum, or the appendix, all of which are uncommon. Despite their sharp ends and elongated shape, bowel perforation caused by ingested fish bones is rarely reported, particularly in patients without intestinal disease. We report a case of 57-year-old female who visited the emergency room with periumbilical pain and no history of underlying intestinal disease or intra-abdominal surgery. Abdominal computed tomography and exploratory laparotomy revealed a small bowel micro-perforation with a 2.7-cm fish bone penetrating the jejunal wall.

关键词： Peritonitis Small bowel perforation Foreign body Fish bone Japanese red rock fish

2D-3D registration for 3D analysis of lower limb alignment in a weight-bearing condition

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Applied Mathematics(A Journal of Chinese Universities) 2018年第1期33卷 59-70页

作者： shim Eungjune KIM Youngjun LEE Deukhee LEE Byung Hoon WOO Sungkyung LEE Kunwoo Center for Bionics Korea Institute of Science and TechnologyKorea Department of Biomedical Engineering University of Science and TechnologyKorea Department of Orthopaedic Surgery Kang-Dong Sacred Heart HospitalHallym University Medical SchoolKorea Mechanical Engineering Seoul National UniversityKorea

X-ray imaging is the conventional method for diagnosing the orthopedic condition of a patient. Computerized Tomography(CT) scanning is another diagnostic method that provides patient’s 3D anatomical information. However, both methods have limitations when diagnosing the whole leg; X-ray imaging does not provide 3D information, and normal CT scanning cannot be performed with a standing posture. Obtaining 3D data regarding the whole leg in a standing posture is clinically important because it enables 3D analysis in the weight bearing *** on these clinical needs, a hardware-based bi-plane X-ray imaging system has been developed; it uses two orthogonal X-ray images. However, such methods have not been made available in general clinics because of the hight cost. Therefore, we proposed a widely adaptive method for 2 D X-ray image and 3D CT scan data. By this method, it is possible to threedimensionally analyze the whole leg in standing posture. The optimal position that generates the most similar image is the captured X-ray image. The algorithm verifies the similarity using the performance of the proposed method by simulation-based experiments. Then, we analyzed the internal-external rotation angle of the femur using real patient data. Approximately 10.55 degrees of internal rotations were found relative to the defined anterior-posterior direction. In this paper, we present a useful registration method using the conventional X-ray image and 3D CT scan data to analyze the whole leg in the weight-bearing condition.

关键词： 2D-3D registration 3D analysis X-ray CT simulated annealing

Potential of nano carriers as cancer cell-specific drug delivery systems in photodynamic therapy

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 8-9页

作者： Chang-Koo shim Ji-Eun Chang College of Pharmacy Seoul National University

Photodynamic therapy (PDT) in combination with chemotherapy has great potential for cancer treatment (1, 2)However,there have been very few attempts to develop cancer-targeted co-delivery systems of photosensitizers and anticancer *** developed hematoporphyrin (HP)-modified doxorubicin(DOX)-loaded bovine serum albumin nanoparticles (HP-NPs) in an attempt to improve the therapeutic effect of PDT in treating liver cancer.

关键词： Photodynamic therapy Hematoporphyrin Doxorubicin Nanoparticles Liver cancer

Disruption of Dhcr7 and Insig1/2 in cholesterol metabolism causes defects in bone formation and homeostasis through primary cilium formation

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Bone Research 2020年第1期8卷 39-52页

作者： Akiko Suzuki Kenichi Ogata Hiroki Yoshioka Junbo shim Christopher AWassif Forbes DPorter Junichi Iwata Department of Diagnostic&Biomedical Sciences The University of Texas Health Science Center at HoustonSchool of DentistryHoustonTXUSA Center for Craniofacial Research The University of Texas Health Science Center at HoustonSchool of DentistryHoustonTXUSA Division of Translational Medicine Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentBethesdaMDUSA Pediatric Research Center The University of Texas Health Science Center at HoustonMcGovern Medical SchoolHoustonTXUSA MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences HoustonTXUSA

Human linkage studies suggest that craniofacial deformities result from either genetic mutations related to cholesterol metabolism or high-cholesterol maternal diets. However, little is known about the precise roles of intracellular cholesterol metabolism in the development of craniofacial bones, the majority of which are formed through intramembranous ossification. Here, we show that an altered cholesterol metabolic status results in abnormal osteogenesis through dysregulation of primary cilium formation during bone formation. We found that cholesterol metabolic aberrations, induced through disruption of either Dhcr7(which encodes an enzyme involved in cholesterol synthesis) or Insig1 and Insig2(which provide a negative feedback mechanism for cholesterol biosynthesis), result in osteoblast differentiation abnormalities. Notably, the primary cilia responsible for sensing extracellular cues were altered in number and length through dysregulated ciliary vesicle fusion in Dhcr7 and Insig1/2 mutant osteoblasts. As a consequence, WNT/β-catenin and hedgehog signaling activities were altered through dysregulated primary cilium ***, the normalization of defective cholesterol metabolism by simvastatin, a drug used in the treatment of cholesterol metabolic aberrations, rescued the abnormalities in both ciliogenesis and osteogenesis in vitro and in vivo. Thus, our results indicate that proper intracellular cholesterol status is crucial for primary cilium formation during skull formation and homeostasis.

关键词： metabolism cholesterol homeostasis

DNA-based artificial dendritic cells for in situ cytotoxic T cell stimulation and immunotherapy

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Bioactive Materials 2022年第9期7卷 160-172页

作者： Quoc-Viet Le Jaiwoo Lee Junho Byun Gayong shim Yu-Kyoung Oh College of Pharmacy and Research Institute of Pharmaceutical Sciences Seoul National University1 Gwanak-roGwanak-guSeoul08826Republic of Korea School of Systems Biomedical Science Soongsil UniversitySeoul06978Republic of Korea

In immunotherapy,ex vivo stimulation of T cells requires significant resources and ***,we report artificial dendritic cell-mimicking DNA microflowers(DM)for programming T cell stimulation in *** mimic dendritic cells,DNA-based artificial dendritic microflowers were constructed,surface-coated with polydopamine,and further modified with anti-CD3 and anti-CD28 antibodies to yield antibody-modified DM(DM-A).The porous structure of DM-A allowed entrapment of the T cell-stimulating cytokine,ineterleukin-2,yielding interleukin-2-loaded DM-A(DM-AI).For comparison,polystyrene microparticles coated with polydopamine and modified with anti-CD3 and anti-CD28 antibodies(PS-A)were *** to PS-A,DM-AI showed significantly greater contact with T cell ***-AI provided the highest ex vivo expansion of cytotoxic T *** injection of DM-AI to tumor tissues induced the recruitment of T cells and expansion of cytotoxic T cells in tumor *** the other groups,model animals injected with DM-AI did not exhibit growth of primary *** of mice with DM-AI also protected against growth of a rechallenged distant tumor,and thus prevented tumor recurrence in this ***-AI has great potential for programmed stimulation of CD8+T *** concept could be broadly extended for the programming of specific T cell stimulation profiles.

关键词： Artificial dendritic cell DNA microflower In situ T cell stimulation Programmed T cell expansion Immunotherapy

A Privacy-Preserving Image Retrieval Based on AC-Coefficients and Color Histograms in Cloud Environment

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Computers, Materials & Continua 2019年第1期58卷 27-43页

作者： Zhihua Xia Lihua Lu Tong Qiu H.J.shim Xianyi Chen Byeungwoo Jeon Jiangsu Engineering Centre of Network Monitoring Jiangsu Collaborative Innovation Center on Atmospheric Environment and Equipment TechnologySchool of Computer and SoftwareNanjing University of Information Science&TechnologyNanjing210044China College of Information&Communication Engineering Sungkyunkwan UniversityKorea

Content based image retrieval(CBIR)techniques have been widely deployed in many applications for seeking the abundant information existed in *** to large amounts of storage and computational requirements of CBIR,outsourcing image search work to the cloud provider becomes a very attractive option for many owners with small ***,owing to the private content contained in images,directly outsourcing retrieval work to the cloud provider apparently bring about privacy problem,so the images should be protected carefully before *** paper presents a secure retrieval scheme for the encrypted images in the YUV color *** this scheme,the discrete cosine transform(DCT)is performed on the Y *** resulting DC coefficients are encrypted with stream cipher technology and the resulting AC coefficients as well as other two color components are encrypted with value permutation and position *** the image owner transmits the encrypted images to the cloud *** receiving a query trapdoor form on query user,the server extracts AC-coefficients histogram from the encrypted Y component and extracts two color histograms from the other two color *** similarity between query trapdoor and database image is measured by calculating the Manhattan distance of their respective ***,the encrypted images closest to the query image are returned to the query user.

关键词： Image retrieval AC-coefficients histogram color histogram discrete cosine transform

Down-scaled regional ocean modeling system (ROMS) for high-resolution coastal hydrodynamics in Korea

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Acta Oceanologica Sinica 2013年第9期32卷 50-61页

作者： LIM Hak-Soo KIM Chang S PARK Kwang-Soon shim Jae Seol CHUN Insik Korea Institute of Ocean Science and Technology Konkuk University

A down-scaled operational oceanographic system is developed for the coastal waters of Korea using a re- gional ocean modeling system （ROMS）. The operational oceanographic modeling system consists of at- mospheric and hydrodynamic models. The hydrodynamic model, ROMS, is coupled with wave, sediment transport, and water quality modules. The system forecasts the predicted results twice a day on a 72 h basis, including sea surface elevation, currents, temperature, salinity, storm surge height, and wave information for the coastal waters of Korea. The predicted results are exported to the web-GIS-based coastal informa- tion system for real-time dissemination to the public and validation with real-time monitoring data using visualization technologies. The ROMS is two-way coupled with a simulating waves nearshore model, SWAN, for the hydrodynamics and waves, nested with the meteorological model, WRE for the atmospheric surface forcing, and externally nested with the eutrophication model, CE-QUAL-ICM, for the water quality. The op- erational model, ROMS, was calibrated with the tidal surface observed with a tide-gage and verified with current data observed by bottom-mounted ADCP or AWAC near the coastal waters of Korea. To validate the predicted results, we used real-time monitoring data derived from remote buoy system, HF-radar, and geostationary ocean color imager （GOCI）. This down-scaled operational coastal forecasting system will be used as a part of the Korea operational oceanographic system （KOOS） with other operational oceanographic systems.

关键词： down-scaled operational oceanographic system regional ocean modeling system wave coupledmodel real-time monitoring system