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Anti-scarring effects of butaprost on human subconjunctival Tenon's fibroblasts

International Journal of Ophthalmology(English edition) 2017年第7期10卷 1028-1033页

作者： Jong Hoon Shin Je hyun Seo jae Ho Jung Tae Woo kim Department of Ophthalmology Pusan National University Hospital Department of Ophthalmology Pusan National University Yangsan Hospital Research Institute for Convergence of Biomedical Science and Technology Pusan National University Yangsan Hospital Department of Ophthalmology Seoul National University College of MedicineSeoul National University Bundang Hospital

AIM： To investigate the toxicity of the E-prostanoid 2（EP2） receptor agonist, butaprost against human subconjunctival（Tenon＇s capsule） fibroblasts, and to determine the underlying mechanism. METHODS： We isolated Tenon＇s fibroblasts from the subconjunctival area of healthy subjects and evaluated the types of EP receptors expressed using quantitative realtime reverse transcription polymerase chain reaction（RTPCR）. The toxicity of butaprost against the fibroblasts was evaluated using methyl thiazolyl tetrazolium and lactic dehydrogenase assays. The inhibition of conjunctival fibroblast proliferation by butaprost was assessed by measuring α-actin levels. The underlying mechanism was assessed by measuring intracellular cyclic adenosine monophosphate（c AMP） levels. Intergroup differences were statistically analyzed using an independent t-test. Densitometry of the Western blot bands was performed using the Image J software. RESULTS： Quantitative real-time RT-PCR revealed that the fibroblast EP2 receptor levels were higher than those of the other EP receptors. Butaprost did not show toxicity against Tenon＇s tissue, but inhibited conjunctival fibroblast proliferation by reducing collagen synthesis. EP2 receptor activation enhanced the c AMP cascade, which might be an important mechanism underlying this ***： Butaprost effectively reduces the subconjunctival scarring response. Given the significanceof wound healing modulation in blebs, butaprost＇s inhibitory effect on subconjunctival Tenon＇s fibroblasts may be beneficial in managing postoperative scarring in glaucoma surgery.

关键词： butaprost Tenon's capsule trabeculectomy fibroblasts

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Expression and self-assembly of Heterocapsa circularisquama RNA virus-like particles synthesized in Pichia pastoris

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Chinese Science Bulletin 2012年第25期57卷 3288-3293页

作者： WU YuanZheng kim Wonduck kim Si-Wou EOM Chi-Yong YANG HeTong SHIN hyun-jae Department of Chemical and Biochemical Engineering Chosun University Gwangju 501-759 Republic of Korea Department of Environmental Engineering Chosun University Gwangju 501-759 Republic of Korea Seoul Center Korea Basic Science Institute Seoul 136-713 Republic of Korea Biotechnology Center of Shandong Academy of Sciences Jinan 250014 China

Heterocapsa circularisquama RNA virus(HcRNAV) is the first single-stranded RNA virus to be characterized that infects *** ability of HcRNAV coat protein(HcRNAV CP) to self-assemble into virus-like particles(VLPs) in vitro suggested that heterologous expression was possible,and that the VLPs might be ideal nanocontainers for the targeted delivery of genes and *** this paper,we report the expression of a codon-optimized HcRNAV 109 CP gene in Pichia pastoris and the production of self-assembled HcRNAV VLPs using large-scale *** HcRNAV 109 CP gene was synthesized according to the codon preference of *** and cloned into a pPICZA *** recombinant plasmid pPICZA-CPsyns was transformed into *** by *** resulting yeast colonies were screened by PCR and analyzed for protein expression by SDS polyacrylamide gel *** large-scale fermentation,the yield of HcRNAV CPsyns reached approximately 2.5 g L 1 within 4 *** HcRNAV VLPs were purified using PEG precipitation followed by cesium chloride density gradient ultracentrifugation,and were subsequently analyzed using UV spectrophotometry and transmission electron *** dye-labeled myoglobin was loaded into the cages of the HcRNAV VLPs and the encapsulation was confirmed by fluorescence *** results point to the possible utilization in pharmacology or nanotechnology of HcRNAV VLPs produced by *** fermentation.

关键词：巴斯德毕赤酵母病毒样颗粒 RNA病毒 pastoris 基因合成自组装透射电子显微镜分析紫外分光光度法

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LOCAL REGULARITY CRITERIA OF A SUITABLE WEAK SOLUTION TO MHD EQUATIONS

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Acta Mathematica Scientia 2017年第4期37卷 1033-1047页

作者： jae-Myoung kim Department of Mathematical Sciences Seoul National UniversitySeoul 08826Republic of Korea

We present a regularity condition of a suitable weak solution to the MHD equations in three dimensional space with slip boundary conditions for a velocity and magnetic vector fields. More precisely, we prove a suitable weak solution are HSlder continuous near boundary provided that the scaled mixed Lx,t^p,q-norm of the velocity vector field with 3/p ＋ 2/q 〈 2, 2 〈 q 〈 ∞ is sufficiently small near the boundary. Also, we will investigate that for this 3 2〈3 solution U ∈ Lx,t^p,q with 1 〈 3＋p ＋2/q＋≤3/2, 3 〈 p 〈 ∞, the Hausdorff dimension of its singular set is no greater than max{p, q}（3/q＋2/q- 1）.

关键词： local regularity criteria suitable weak solution MHD equations

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LOCAL EXISTENCE AND BLOW-UP CRITERION OF 3D IDEAL MAGNETOHYDRODYNAMICS EQUATIONS

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Acta Mathematica Scientia 2018年第6期38卷 1759-1766页

作者： jae-Myoung kim Center for Mathematical Analysis & Computation Yonsei University

We investigate the local existence of smooth solutions of a 3D ideal magneto-hydrodynamics （MHD） equations in a bounded domain and give a blow-up criteria to thisequations with respect to vorticists.

关键词： ideal magnetohydrodynamics equations blow-up criterion bounded domain

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The effect of diet-induced insulin resistance on DNA methylation of the androgen receptor promoter in the penile cavernosal smooth muscle of mice

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Asian Journal of Andrology 2013年第4期15卷 487-491,I0007页

作者： Jin-Wook kim Mi-Mi Oh Cheol-Yong Yoon jae-hyun Bae Je-Jong kim Du-Geon Moon Department of Urology Korea University Medical Center Guro Hospital Seou1152-703 Korea and Department of Urology Korea University College of Medicine Seou1152 703 Korea

Population studies have suggested an association between diabetes and the symptoms of testosterone deficiency. Recently, the expression of the androgen receptor （AR） has been shown to be decreased in diabetic patients. Furthermore, diabetes has been shown to induce global methylation. In this study, we used an animal model to investigate whether diabetes results in increased methylation of the AR promoter and whether these changes are associated with the decreased expression of AR in penile cavernosal smooth muscle tissue. Twenty C57BL/6J mice were divided into two groups, receiving either high- （mature diabetic） or low- （mature control） caloric meals for 14 weeks. Another 10 mice were killed at 1 week （young control）. Animals in the mature diabetic group showed decreased testosterone levels, although this was not statistically significant. In both control groups, no significant methylation was observed in the AR promoter region CpG island （-85 to ＋339）. In the mature diabetic group, significant methylation was observed at ＋ 185 and ＋200 of the AR promoter. These changes were associated with increased homeostatic model assessment for insulin resistance （HOMA-IR） and decreased corpus cavernosal tissue mass and expression of AR mRNA and protein. We conclude that in these animals, insulin resistance increased the methylation of the GC-rich regions of the AR promoter, leading to decreased AR expression.

关键词： androgen receptor (AR) diabetes DNA methylation

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Pancreatic adenocarcinosarcoma of monoclonal origin:A case report

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World Journal of Gastroenterology 2014年第35期20卷 12682-12686页

作者： hyun Seon kim Jin Il kim Minyoung Jeong jae hyun Seo Il Kyu kim Dae Young Cheung Tae-Jung kim Chang Suk Kang Department of Internal Medicine The Catholic University of KoreaCollege of MedicineYeouido St.Mary’s Hospital Hospital Pathology Department The Catholic University of KoreaCollege of MedicineYeouido St.Mary’s Hospital

Adenocarcinosarcoma, a neoplasm containing both carcinomatous and sarcomatous components, is a rare form of a cancer and the pathophysiology is currently poorly understood. Moreover, definitive treatment guidelines for this disease have not yet been established. Pancreatic adenocarcinosarcoma is even more rare and the prognosis is fatal. Here, we report a case of a 77-year-old male with pancreatic adenocarcinosarcoma and metastasis to the liver. The patient presented at our hospital with uncontrolled glucose levels and diabetes mellitus. The patient’s laboratory findings were unremarkable with the exception of elevated carbohydrate antigen 19-9 levels. Biopsies of the tumors in the pancreas and the liver revealed two types of tumors: pancreatic adenocarcinoma and a poorly differentiated sarcoma. To determine if KRAS mutations were present, we performed a peptide nucleic acid (PNA) clamp PCR-based assay. DNA sequencing by PNA clamp PCR identified a point mutation in codon 12 of exon 2 within KRAS from both tumor types. Because the KRAS mutation is observed in both tumor components, our findings support a monoclonal tumor origin followed by subsequent divergent differentiation into the sarcomatous and carcinomatous tumor populations. After we considered the patient’s status and the late stage of tumor detection, gemcitabine chemotherapy was administered.

关键词： Pancreas Adenocarcinosarcoma Metastasis KRAS DNA sequencing

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Molecular mechanisms of ZD1839 (Iressa)-induced apoptosis in human leukemic U937 cells

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Acta Pharmacologica Sinica 2007年第8期28卷 1205-1214页

作者： Dong-oh MOON Moon-ok kim jae-dong LEE Yung-hyun CHOI Min-ki LEE Gi-young kim Faculty of Applied Marine Science Cheju National UniversityJeju Special Self-Governing Province 690-756Republic of Korea Department of Microbiology Pusan National UniversityBusan 614-052Republic of Korea Department of Biochemistry Dongeui University College of Oriental MedicineBusan 614-052Republic of Korea Department of Internal Medicine Pusan National University College o MedicineBusan 602-739Republic of Korea

Aim:To investigate the molecular mechanisms of ZD1839-induced apoptosis inhuman leukemic U937 ***:The inhibition of human leukemic U937 cellgrowth was assessed by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphnyl-2H-tetrazolimbromide（MTT）assays,lactate dehydrogenase（LDH）release,and cell *** expression of anti- and pro-apoptotic proteins was detected byWestern blot ***:This study demonstrated that ZD 1839 inducedapoptosis in leukemic U937 cells by the downregulation of Bcl-2,caspase activa-tion and subsequent apoptotic *** with ZD 1839 and the caspase-3 inhibitor z-DEVD-fmk blocked apoptosis,indicating that caspase-3 activation isat least partially responsible for ZD1839-induced *** ectopic expres-sion of Bcl-2 attenuated caspase-3 activation,PARP cleavage,and subsequentindicators of apoptosis,including sub-G1DNA content and LDH *** indicate that the downregulation of Bcl-2 plays a major role in the initiationof ZD1839-induced apoptosis,and that the activation of a caspase cascade isinvolved in the execution of ***,ZD 1839 treatment triggeredthe activation of p38 mitogen-activated protein kinase（MAPK）and the down-regulation of c-Jun-N-terminal kinase（JNK）,extracellular signal-regulated kinase（ERK）and phosphatidyl inositol 3-kinase（PI3K）/*** inhibition of the ERKand PI3K/Akt pathways also significantly increased cellular ***:ZD 1839 activated caspase-3 and the inhibited Bcl-2 in human leukemic U937 cellsthrough the downregulation of the ERK and PI3K/Akt pathways.

关键词： ZD1839(Iressa) apoptosis caspase-3 Bcl-2 mitogen-activated protein kinases

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Clinical features of gastric adenoma detected within 3 years after negative screening endoscopy in Korea

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Gastroenterology Report 2023年第1期11卷 406-411页

作者： hyun Young kim Department of Internal Medicine Seoul National University Bundang HospitalSeongnam-siGyeonggi-doKorea

Background:Early detection and management of gastric adenoma are important for preventing gastric *** present study aimed to evaluate the predictors of missed gastric adenoma on screening endoscopy in Korea and identify the risk factors associated with interval precancerous gastric ***:All cases of gastric adenomas diagnosed via screening endoscopy between 2007 and 2019 were *** them,those who had undergone endoscopy within 3 years were included in the present *** gastric adenoma was defined as gastric adenoma diagnosed within 3 years after negative screening ***:In total,295 cases of gastric adenoma were *** these,95(32.2%)were missed gastric adenoma cases(mean age,60.6 years;average interval between final and index endoscopies,12.6months);the remaining 200(67.8%)were newly detected adenoma *** analysis revealed thatmale sex,endoscopist experience,observation time,and presence of gastric intestinalmetaplasia(pathologically proven)were associated with missed gastric *** analysis revealed that gastric intestinal metaplasia(odds ratio[OR],2.736;95%confidence interval[CI],1.320–5.667;P=0.007)and shorter observation time of the index screening endoscopy(B,0.011;OR,0.990;95%CI,0.986–0.993;P<0.001)were independent risk factors for missed gastric *** optimal cut-off for the observation time for detecting gastric adenoma was 3.53minutes(area under curve,0.738;95%CI,0.677–0.799;P<0.001).Conclusions:Gastric intestinal metaplasia is an indication of missed gastric ***,careful inspection of gastric mucosa with gastric intestinal metaplasia and proper observation time can lower the possibility of missing the gastric adenoma during screening.

关键词： gastric adenoma screening endoscopy gastric intestinal metaplasia observation time

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Photocatalysts for degradation of dyes in industrial effluents:Opportunities and challenges

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Nano Research 2019年第5期12卷 955-972页

作者： Hassan Anwer Asad Mahmood Jechan Lee Ki-hyun kim jae-Woo Park Alex C.K.Yip Department of Civil and Environmental Engineering Hanyang University222 Wangsimni-roSeoul 04763Republic of Korea Department of Environmental and Safety Engineering Ajou UniversitySuwon 16499Republic of Korea Department of Chemical and Process Engineering University of CanterburyChristchurch 8041New Zealand

Discharging dye contaminants into water is a major concern around the *** a variety of methods to treat dye-contaminated water,photocatalytic degradation has gained attention as a tool for treating the colored ***,we review the recent advancements in photocatalysis for dye degradation in industrial effluents by categorizing photocatalyst materials into three *** generation photocatalysts are composed of single-component materials (e.g.,TiO2,ZnO,and CdS),while second generation photocatalysts are composed of multiple components in a suspension (e.g.,WO3/NiWO4,BiOl/ZnTiOa,and C3N4/Ag3VO4).Photocatalysts immobilized on solid substrates are regarded as third generation materials (e.g.,FTO/WO3-ZnO,Steel/TiO2-WO3,and Glass/P-TiO2).Photocatalytic degradation mechanisms,factors affecting the dye degradation,and the lesser-debated uncertainties related to the photocatalysis are also discussed to offer better insights into environmental ***,quantum yields of different photocatalysts are calculated,and a performance evaluation method is proposed to compare photocatalyst systems for dye ***,we discuss the present limitations of photocatalytic dye degradation for field applications and the future of the technology.

关键词： photocatalyst dye wastewater degradation mechanism performance evaluation

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Hyperreflective photonic crystals created by shearing colloidal dispersions at ultrahigh volume fraction

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Microsystems & Nanoengineering 2024年第1期10卷 161-173页

作者： Minji kim Jong Bin kim Shin-hyun kim Department of Chemical and Biomolecular Engineering Korea Advanced Institute of Science and Technology(KAIST)Daejeon 34141Republic of Korea

Colloidal crystallization serves as one of the most economic and scalable production methods for photonic ***,insufficient optical performance,nonuniformity and low reproducibility remain challenges for advanced high-value *** this study,we optimally formulate a photocurable dispersion of silica particles and apply shear flow to unify the orientation of the colloidal crystals,ensuring high optical performance and *** silica particles experience strong repulsion at ultrahigh volume fractions of 50%but demonstrate low mobility,leading to polycrystalline *** shear flow to the dispersions allows the silica particles to rearrange into larger crystalline domains with a unidirectional orientation along the *** shear-induced structural change produces absolute reflectivity at the stopband as high as 90%and a high transparency of 90%at off-resonant wavelengths with minimal diffusive ***,the strong interparticle repulsion ensures a uniform volume fraction of particles throughout the dispersion,reducing deviations in the optical *** intricately micropattern the photocurable dispersions using ***,the photonic films and patterns can be stacked to form multiple layers,displaying mixed structural colors and multiple reflectance peaks without sacrificing *** superior photonic materials hold promise for various optical applications,including optical components and anticounterfeiting patches.

关键词： properties. dispersion reflectivity

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