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A crucial role of neutrophil extracellular traps in pulmonary infectious diseases

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Chinese Medical Journal Pulmonary and Critical Care Medicine 2024年第1期2卷 34-41页

作者： Ting Pan jae woo Lee Shanghai Key Laboratory of Lung Inflammation and Injury Department of Pulmonary MedicineZhongshan HospitalFudan UniversityShanghai 200032China Department of Anesthesiology University of California Los AngelesLos AngelesCA 90230USA

Neutrophil extracellular traps(NETs),extrusions of intracellular DNA with attached granular material that exert an antibacterial effect through entangling,isolating,and immobilizing microorganisms,have been extensively studied in recent *** primary role of NETs is to entrap and facilitate the killing of bacteria,fungi,viruses,and parasites,preventing bacterial and fungal *** formation has been described in many pulmonary diseases,including both infectious and *** are considered a double-edged *** innate immune cells,neutrophils release NETs to kill pathogens and remove cellular ***,the dele-terious effects of excessive NET release in lung disease are particularly important because NETs and by-products of NETosis can directly induce epithelial and endothelial cell death while simultaneously inducing inflammatory cytokine secretion and immune-mediated ***,NET formation must be tightly regulated to preserve the anti-microbial capability of NETs while minimizing damage to the *** this review,we summarized the recent updates on the mechanism of NETs formation and pathophysiology associated with excessive NETs,aiming to provide insights for research and treatment of pulmonary infectious diseases.

关键词： Pulmonary infectious diseases Neutrophils Neutrophil extracellular traps(NETs) NETosis

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok Kwon Dae-Jin Jung Dae-Hyun Kim Jun-Koo Yi jae-Jung Ha Ji-Hyun Lee Seong-Beom Lee woo-jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites

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Cancer Communications 2024年第1期44卷 47-75页

作者： Ji Eon Kim So-Young Park Chulhwan Kwak Yoonji Lee Dae-Geun Song jae woo Jung Haesong Lee Eun-Ae Shin Yangie Pinanga Kyung-hee Pyo Eun Hae Lee Wonsik Kim Soyeon Kim Chang-Duck Jun Jeanho Yun Sun Choi Hyun-woo Rhee Kwang-Hyeon Liu Jung Weon Lee Department of Pharmacy College of PharmacySeoul National UniversitySeoulRepublic of Korea Research Institute of Pharmaceutical Sciences College of PharmacySeoul National UniversitySeoulRepublic of Korea BK21 FOUR Community-Based Intelligent Novel Drug Discovery Education Unit College of Pharmacy and Research Institute of Pharmaceutical SciencesKyungpook National UniversityDaeguRepublic of Korea Department of Chemistry Seoul National UniversitySeoulRepublic of Korea College of Pharmacy Chung-Ang UniversitySeoulRepublic of Korea Natural Product Informatics Research Center Korea Institute of Science and Technology(KIST)Gangneung-siGangwon-doRepublic of Korea School of Life Sciences Gwangju Institute of Science and Technology(GIST)GwangjuRepublic of Korea Department of Biochemistry College of MedicineDong-A UniversityBusanRepublic of Korea Global AI Drug Discovery Center College of Pharmacy and Graduate School of Pharmaceutical SciencesEwha Womans UniversitySeoulRepublic of Korea Interdisciplinary Program in Genetic Engineering Seoul National UniversitySeoulRepublic of Korea

Background:Transmembrane 4 L six family member 5(TM4SF5)translocates subcellularly and functions metabolically,although it is unclear how intracellu-lar TM4SF5 translocation is linked to metabolic *** is thus of interests to understand how the traffic dynamics of TM4SF5 to subcellular endosomal membranes are correlated to regulatory roles of ***:Here,we explored the metabolic significance of TM4SF5 localization at mitochondria-lysosome contact sites(MLCSs),using in vitro cells and in vivo animal systems,via approaches by immunofluorescence,proximity labelling based proteomics analysis,organelle reconstitution ***:Upon extracellular glucose repletion following depletion,TM4SF5 became enriched at MLCSs via an interaction between mitochondrial FK506-binding protein 8(FKBP8)and lysosomal *** labeling showed molecular clustering of phospho-dynamic-related protein I(DRP1)and certain mitophagy receptors at TM4SF5-enriched MLCSs,leading to mitochondrial fis-sion and ***4SF5 bound NPC intracellular cholesterol transporter 1(NPC1)and free cholesterol,and mediated export of lysosomal cholesterol to mitochondria,leading to impaired oxidative phosphorylation but intact tri-carboxylic acid(TCA)cycle andβ-*** mouse models,hepatocyte Tm4sf5 promoted mitophagy and cholesterol transport to mitochondria,both with positive relations to liver ***:Our findings suggested that TM4SF5-enriched MLCSs regu-late glucose catabolism by facilitating cholesterol export for mitochondrial reprogramming,presumably while hepatocellular carcinogenesis,recapitulating aspects for hepatocellular carcinoma metabolism with mitochondrial repro-gramming to support biomolecule synthesis in addition to glycolytic energetics.

关键词： cholesterol fluorescent imaging glucose catabolism hepatocellular carcinogenesis mem-brane contact sites mitochondria function mitophagy oxidative phosphorylation protein-protein interaction tetraspanin

Efficient multi-response adaptive sampling algorithm for construction of variable-fidelity aerodynamic tables

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Chinese Journal of Aeronautics 2019年第3期32卷 547-558页

作者： Maxim TYAN jae-woo LEE Department of Aerospace Information Engineering Konkuk University

Adaptive sampling is an iterative process for the construction of a global approximation model. Most of engineering analysis tools computes multiple parameters in a single run. This research proposes a novel multi-response adaptive sampling algorithm for simultaneous construction of multiple surrogate models in a time-efficient and accurate manner. The new algorithm uses the Jackknife cross-validation variance and a minimum distance metric to construct a sampling criterion function. A weighted sum of the function is used to consider the characteristics of multiple surrogate models. The proposed algorithm demonstrates good performance on total 22 numerical problems in comparison with three existing adaptive sampling algorithms. The numerical problems include several two-dimensional and six-dimensional functions which are combined into singleresponse and multi-response systems. Application of the proposed algorithm for construction of aerodynamic tables for 2 D airfoil is demonstrated. Scaling-based variable-fidelity modeling is implemented to enhance the accuracy of surrogate modeling. The algorithm succeeds in constructing a system of three highly nonlinear aerodynamic response surfaces within a reasonable amount of time while preserving high accuracy of approximation.

关键词： Adaptive sampling Aerodynamic database Multi-response system Surrogate modeling Variable fidelity modeling

k-Nearest Neighbor Query Processing Algorithms for a Query Region in Road Networks

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Journal of Computer Science & Technology 2013年第4期28卷 585-596页

作者： Hyeong-Il Kim jae-woo Chang Department of Computer Engineering Chonbuk National University Cloud Open R&D Center Chonbuk National University

Recent development of wireless communication technologies and the popularity of smart phones .are making location-based services （LBS） popular. However, requesting queries to LBS servers with users＇ exact locations may threat the privacy of users. Therefore, there have been many researches on generating a cloaked query region for user privacy protection. Consequently, an efficient query processing algorithm for a query region is required. So, in this paper, we propose k-nearest neighbor query （k-NN） processing algorithms for a query region in road networks. To efficiently retrieve k-NN points of interest （POIs）, we make use of the Island index. We also propose a method that generates an adaptive Island index to improve the query processing performance and storage usage. Finally, we show by our performance analysis that our k-NN query processing algorithms outperform the existing k-Range Nearest Neighbor （kRNN） algorithm in terms of network expansion cost and query processing time.

关键词： island index k-nearest neighbor query processing scheme locatiombased service road network

Biomass seaweed-derived n itrogen self-doped porous carb on anodes for sodium-ion batteries:Insights into the structure and electrochemical activity

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Journal of Energy Chemistry 2022年第1期31卷 286-295,I0008页

作者： Chenrayan Senthil jae woo Park Nitheesha Shaji Gyu Sang Sim Chang woo Lee Department of Chemical Engineering(Integrated Engineering)&Center for the SMART Energy Platform College of EngineeringKyung Hee University1732 Deogyeong-daeroCihuengYonginGyeonggi 17104South Korea

Sustainable transformation and efficient utilization of biomasses and their derived materials are environ-mentally as well as economically compliant *** seaweed-derived nitrogen self-doped porous carbon with tailored surface area and pore structures are prepared through carb on izatio n and *** in fluence of carb on ization temperature on morphology,surface area,and heteroatom dopants are investigated to optimize sodium-ion storage ***-derived nitrogen selfdoped activated carbon(SAC)as anode materials for sodium-ion batteries exhibits remarkable reversible capacity of 303/192 mAh g^(-1) after 100/500 cycles at current densities of 100/200 mA g^(-1) respectively,and a good rate *** interconnected and porous conducting nature along with the heteroatom dopant role in creating defective sites and charge stabilization are favorable for ion storage and diffusion and electron transport,indicating the electrodes can offer improved electrochemical *** addition,post-mortem analysis of the cycled carbon electrodes through ex-situ tools demonstrates the sodium-ion storage mechanism.

关键词： Biomass Heteroatom self-doping Porous carbon Anode Sodium-ion batteries Post-mortem ex-situ analysis

Aerodynamic design optimization of helicopter rotor blades including airfoil shape for hover performance

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Chinese Journal of Aeronautics 2013年第1期26卷 1-8页

作者： Ngoc Anh Vu jae woo Lee Jung Il Shu Department of Aerospace Engineering Ho Chi Minh City University of Technology Department of Aerospace Information Engineering Konkuk University

This study proposes a process to obtain an optimal helicopter rotor blade shape for aerodynamic performance in hover flight. A new geometry representation algorithm which uses the class function/shape function transformation （CST） is employed to generate airfoil coordinates. With this approach, airfoil shape is considered in terms of design variables. The optimization process is constructed by integrating several programs developed by author. The design variables include twist, taper ratio, point of taper initiation, blade root chord, and coefficients of the airfoil distribution function. Aerodynamic constraints consist of limits on power available in hover and forward flight. The trim condition must be attainable. This paper considers rotor blade configuration for the hover flight condition only, so that the required power in hover is chosen as the objective function of the optimization problem. Sensitivity analysis of each design variable shows that airfoil shape has an important role in rotor performance. The optimum rotor blade reduces the required hover power by 7.4% and increases the figure of merit by 6.5%, which is a good improvement for rotor blade design.

关键词： Airfoil Design optimization Helicopter design Hover performance Planforms Rotor blades design

Anesthetics management of a renal angiomyolipoma using pulse pressure variation and non-invasive cardiac output monitoring: A case report

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World Journal of Clinical Cases 2022年第24期10卷 8656-8661页

作者： woo jae Jeon woo Jong Shin Young Joon Yoon Chan woo Park jae Hang Shim Sang Yun Cho Department of Anesthesiolgy and Pain Medicine Hanyang University Guri HospitalGuri-si 471-701Gyeonggi-doSouth Korea

BACKGROUND Hypovolemic shock can lead to life-threatening organ dysfunction,and adequate fluid administration is a fundamental ***,parameters such as vital signs,central venous pressure,and urine output have been used to estimate intravascular ***,pulse pressure variation(PPV)and non-invasive cardiac monitoring devices have been *** this case report,we introduce a patient with massive active bleeding from giant renal angiomyolipoma(AML).During emergent nephrectomy,we used non-invasive cardiac monitoring with CSN-1901(Nihon Kohden,Tokyo,Japan)and PPV to evaluate the patient's intravascular volume status to achieve optimal fluid *** SUMMARY A 30-year-old male patient with giant AML with active bleeding was referred to the emergency room complaining of severe abdominal pain and spontaneous abdominal *** was diagnosed by computed tomography,and emergent nephrectomy was *** bleeding was expected so we decided to use non-invasive cardiac monitoring and PPV to assist fluid therapy because they are relatively easy and fast compared to invasive cardiac *** the surgery,6000 mL of estimated blood loss *** with the patient's vital signs and laboratory results,we monitored cardiac output,cardiac output,stroke volume,stroke volume index with a non-invasive cardiac monitoring device,and PPV using an intra-arterial catheter to evaluate intravascular volume status of the patient to compensate for massive *** In addition to traditional parameters,non-invasive cardiac monitoring and PPV are useful methods to evaluate patient's intravascular volume status and provideguidance for intraoperative management of hypovolemic shock patients.

关键词： Renal angiomyolipoma Pulse pressure variation Cardiac output Case report

Development of genome engineering technologies in cattle: from random to specific

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Journal of Animal Science and Biotechnology 2018年第2期9卷 285-293页

作者： Soo-Young Yum Ki-Young Youn woo-jae Choi Goo Jang Department of Veterinary Clinical Science College of Veterinary Medicine and the Research Institute of Veterinary Science Seoul National University Farm Animal Clinical Training and Research Center Institute of Green Bio Science Technology Seoul National University Emergence Center for Food-Medicine Personalized Therapy System Advanced Institutes of Convergence Technology Seoul National University College of Veterinary Medicine Seoul National University

The production of transgenic farm animals(e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos(zygotes) followed by embryo transfer into a recipient was performed because of the ease of the ***, as this approach resulted in severe mosaicism and has a low efficiency, it is not typically employed in the cattle as priority, unlike in mice. To overcome the above issue with micro-injection in cattle, somatic cell nuclear transfer(SCNT) was introduced and successfully used to produce cloned livestock. The application of SCNT for the production of transgenic livestock represents a significant advancement, but its development speed is relatively slow because of abnormal reprogramming and low gene targeting efficiency. Recent genome editing technologies(e.g.,ZFN, TALEN, and CRISPR-Cas9) have been rapidly adapted for applications in cattle and great results have been achieved in several fields such as disease models and bioreactors. In the future, genome engineering technologies wil accelerate our understanding of genetic traits in bovine and wil be readily adapted for bio-medical applications in cattle.

关键词： Cattle CRISPR-Cas9 Genome engineering technologies Transgenesis Transposon