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Cuproptosis mediates copper-induced testicular spermatogenic cell death

Asian Journal of Andrology 2024年第3期26卷 295-301页

作者： Jing-Yi Zhang Xu-jun Yu jun-jun Li Yao Xiao Guang-Sen Li fang Yang Liang Dong Chengdu University of Traditional Chinese Medicine Chengdu 611137China Chengdu Fifth People’s Hospital The Fifth People’s Hospital of Chengdu University of Traditional Chinese MedicineChengdu 611130China TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province Hospital of Chengdu University of Traditional Chinese MedicineChengdu 610072China Department of Surgery The Reproductive and Women-Children HospitalChengdu University of Traditional Chinese MedicineChengdu 610041China

Cuproptosis,a novel mechanism of programmed cell death,has not been fully explored in the context of spermatogenic *** investigated the potential involvement of cuproptosis in spermatogenic cell death using a mouse model of copper *** male Institute of Cancer Research(ICR)mice were randomly divided into four groups that received daily oral gavage withsodium chloride(control)or copper sulfate(CuSO_(4))at 50 mg kg^(−1),100 mg kg^(−1),or 200 mg kg^(−1),for 42 consecutive *** to copper overload exhibited a disruption in copper ***,significant upregulated expression of keycuproptosis factors was accompanied by a significant rise in the rates of testicular tissue cell *** revealed the presence of ferredoxin 1(Fdx1)in Sertoli cells,Leydig cells,and spermatogenic cells at various stages oftesticular development,and the Fdx1-positive staining area was significantly increased in copper-overloaded *** and decreased adenosine triphosphate levels were also observed,further implicating mitochondrial damage *** analyses revealed pathological lesions and blood−testis barrier destruction in the testicular tissue,accompaniedby decreased sperm concentration and motility,in copper-overloaded *** summary,our results indicate that copper-overloadedmice exhibit copper homeostasis disorder in the testicular tissue and that cuproptosis participates in spermatogenic cell *** findings provide novel insights into the pathogenic mechanisms underlying spermatogenic cell death and provide initialexperimental evidence for the occurrence of cuproptosis in the testis.

关键词： blood−testis barrier cuproptosis ferredoxin 1 spermatogenic cell death

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Insights into postural orthostatic tachycardia syndrome after COVID-19 in pediatric patients

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World Journal of Pediatrics 2024年第3期20卷 201-207页

作者： Ya-Xi Cui jun-Bao Du Hong-fang Jin Department of Pediatrics Peking University First HospitalNo.1 Xi-an Men StreetWest DistrictBeijing 100034China State Key Laboratory of Vascular Homeostasis and Remodeling Peking UniversityBeijing 100191China

The pandemic caused by the novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)profoundly threatened human health globally and greatly impacted the world economy[1].While most patients recovered,some suff... 详细信息

关键词： acute patients tachycardia

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A positive feedback inhibition of isocitrate dehydrogenase 3β on paired-box gene 6 promotes Alzheimer-like pathology

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2324-2340页

作者： Xin wang Qian Liu Hai-tao Yu Jia-zhao Xie jun-ning Zhao Zhi-ting fang Min Qu Yao Zhang Ying Yang Jian-Zhi wang Department of Pathophysiology School of Basic MedicineKey Laboratory of Education Ministry of China/Hubei Province for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Department of Fundamental Medicine Wuxi School of MedicineJiangnan UniversityWuxiJiangsu 214122China Hubei Provincial Key Laboratory for Applied Toxicology Hubei Provincial Center for Disease Control and PreventionHubei Provincial Academy of Preventive MedicineWuhan 430000China Endocrine Department of Liyuan Hospital Key Laboratory of Ministry of Education of China for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430077China Co-innovation Center of Neuroregeneration Nantong UniversityNantong 226000China

Impaired brain glucose metabolism is an early indicator of Alzheimer’s disease(AD);however,the fundamental mechanism is *** this study,we found a substantial decline in isocitrate dehydrogenase 3β(IDH3β)levels,a critical tricarboxylic acid cycle enzyme,in AD patients and AD-transgenic mice’s *** investigations demonstrated that the knockdown of IDH3βinduced oxidation-phosphorylation uncoupling,leading to reduced energy metabolism and lactate *** resulting increased lactate,a source of lactyl,was found to promote histone lactylation,thereby enhancing the expression of paired-box gene 6(PAX6).As an inhibitory transcription factor of IDH3β,the elevated PAX6 in turn inhibited the expression of IDH3β,leading to tau hyperphosphorylation,synapse impairment,and learning and memory deficits resembling those seen in *** AD-transgenic mice,upregulating IDH3βand downregulating PAX6 were found to improve cognitive functioning and reverse AD-like ***,our data suggest that impaired oxidative phosphorylation accelerates AD progression via a positive feedback inhibition loop of IDH3β-lactate-PAX6-IDH3β.Breaking this loop by upregulating IDH3βor downregulating PAX6 attenuates AD neurodegeneration and cognitive impairments.

关键词： metabolism Alzheimer impaired

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Aspartoacylase suppresses prostate cancer progression by blocking LYN activation

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Military Medical Research 2024年第2期11卷 180-205页

作者： Hong Weng Kang-Ping Xiong wang wang Kai-Yu Qian Shuai Yuan Gang wang fang Yu jun Luo Meng‑Xin Lu Zhong‑Hua Yang Tao Liu Xing Huang Hang Zheng Xing-Huan wang Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Evidence‑Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biological Repositories Zhongnan Hospital of Wuhan UniversityWuhan 430071China Hubei Key Laboratory of Tumor Biological Behaviors Wuhan 430071China Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Pathology and Molecular Diagnostics Wuhan UniversityWuhan 430071China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Background:Globally,despite prostate cancer(PCa)representing second most prevalent malignancy in male,the precise molecular mechanisms implicated in its pathogenesis remain ***,elucidating the key molecular regulators that govern disease progression could substantially contribute to the establishment of novel therapeutic strategies,ultimately advancing the management of ***:A total of 49 PCa tissues and 43 adjacent normal tissues were collected from January 2017 to December 2021 at Zhongnan Hospital of Wuhan *** advanced transcriptomic methodologies were employed to identify differentially expressed mRNAs in *** expression of aspartoacylase(ASPA)in PCa was thoroughly evaluated using quantitative real-time PCR and Western blotting *** elucidate the inhibitory role of ASPA in PCa cell proliferation and metastasis,a comprehensive set of in vitro and in vivo assays were conducted,including orthotopic and tumor-bearing mouse models(n=8 for each group).A combination of experimental approaches,such as Western blotting,luciferase assays,immunoprecipitation assays,mass spectrometry,glutathione S-transferase pulldown experiments,and rescue studies,were employed to investigate the underlying molecular mechanisms of ASPA's action in *** Student‘s t-test was employed to assess the statistical significance between two distinct groups,while one-way analysis of variance was utilized for comparisons involving more than two groups.A two-sided P<0.05 was deemed to indicate statistical ***:ASPA was identified as a novel inhibitor of PCa *** expression of ASPA was found to be significantly down-regulated in PCa tissue samples,and its decreased expression was independently associated with patients’prognosis(HR=0.60,95%CI 0.40–0.92,P=0.018).Our experiments demonstrated that modulation of ASPA activity,either through gain-or loss-of-function,led to the suppression or enhancement of PCa cell proliferation,mig

关键词： Prostate cancer Aspartoacylase LYN JNK AP-1 C-jun Phosphorylation

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Backward scattering of laser plasma interactions from hundreds-of-joules broadband laser on thick target

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Matter and Radiation at Extremes 2024年第1期9卷 5-14页

作者： Peipei wang Honghai An Zhiheng fang jun Xiong Zhiyong Xie Chen wang Zhiyu He Guo Jia Ruirong wang Shu Zheng Lan Xia Wei Feng Haitao Shi Wei wang Jinren Sun Yanqi Gao Sizu Fu Shanghai Institute of Laser Plasma CAEPShanghai 201899People’s Republic of China

The use of broadband laser technology is a novel approach for inhibiting processes related to laser plasma interactions(LPIs).In this study,several preliminary experiments into broadband-laser-driven LPIs are carried out using a newly established hundreds-of-joules broadband second-harmonic-generation laser *** direct comparison with LPI results for a traditional narrowband laser,the actual LPI-suppression effect of the broadband laser is *** broadband laser had a clear suppressive effect on both back-stimulated Raman scattering and back-stimulated Brillouin scattering at laser intensities below 1×10^(15) W cm^(−2).An abnormal hot-electron phenomenon is also investigated,using targets of different thicknesses.

关键词： scattering laser hundreds

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Impact of atmospheric circulations on droughts and drought propagation over China

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Science China Earth Sciences 2024年第8期67卷 2633-2648页

作者： Ziye GU Lei GU Jiabo YIN Wei fang Lihua XIONG jun GUO Ziyue ZENG jun XIA School of Civil and Hydraulic Engineering Huazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Water Resources Engineering and Management Wuhan 430072China Changjiang River Scientific Research Institute Wuhan 430010China

The GRACE satellite mission provides a new approach for monitoring,tracking,and assessing drought conditions by detecting changes in Earth's gravitational fields and inversing signals of terrestrial water storage *** studies of terrestrial water storage anomalies related droughts paid rare attention to the behind atmospheric physical mechanisms,nor quantified the risk propagation patterns between terrestrial water storage deficits and hydrological or agricultural *** this study,we first extract terrestrial water storage(TWS) droughts,hydrological droughts,and agricultural droughts by using multiple variables including TWS from the GRACE/GRACE-FO satellites,runoff and soil moisture from the ERA5-Land reanalysis *** then identify key atmospheric and oceanic oscillation indices affecting water deficits by employing machine learning *** characterize the joint distributions between drought duration and severity by using the Copula function and quantify the risk propagation of hydrological and agricultural droughts to TWS *** results show that:(1) From 2002 to 2021,there is a significant decrasing trend of TWS in China;the WPIO group atmospheric circulation indices(sea surface temperature index within the Western Pacific and Indian Ocean) contributes the most to ***,the sea surface temperature anomalies within the NINO 3.4 region as well as the Western Pacific Warm Pool area index show significantly positive correlation relationships with TWS in southern China;(2) The northwestern China,the Yangtze River basin,and the downstream of the Pearl River basin are the hotspots of TWS ***,the hotspots of hydrological droughts locate in northwestern China and the hotspots of agricultural droughts locate in South China and eastern Tibetan Plateau;(3) The elastic coefficients of hydrological droughts propagating to TWS droughts are higher than those of agricultural droughts,indicating that the TWS droughts

关键词： GRACE satellites Drought Drought propagation Atmospheric circulation Copula function

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FPGA-based position reconstruction method for neutron beam flux spatial distribution measurement in BNCT

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Nuclear Science and Techniques 2024年第3期35卷 96-108页

作者： Wei Jiang Ping Cao Yi-Ming Wu Xian-Ke Liu Zhu-jun fang Zhi-Yong Zhang Bin Shi jun Chen State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230026China National Key Laboratory for Metrology and Calibration Techniques China Institute of Atomic EnergyBeijing 102413China

A new measurement method for the spatial distribution of neutron beam flux in boron neutron capture therapy(BNCT)is being developed based on the two-dimensional Micromegas *** address the issue of long processing times in traditional offline position reconstruction methods,this paper proposes a field programmable gate array based online position reconstruction method utilizing the micro-time projection chamber *** method encapsulates key technical aspects:a self-adaptive serial link technique built upon the dynamical adjustment of the delay chain length,fast sorting,a coordinate-matching technique based on the mapping between signal timestamps and random access memory(RAM)addresses,and a precise start point-merging technique utilizing a circular combined *** performance test of the selfadaptive serial link shows that the bit error rate of the link is better than 10-12 at a confidence level of 99%,ensuring reliable data *** experiment utilizing the readout electronics and Micromegas detector shows a spatial resolution of approximately 1.4 mm,surpassing the current method’s resolution level of 5 *** beam experiment confirms that the readout electronics system can obtain the flux spatial distribution of neutron beams online,thus validating the feasibility of the position reconstruction *** online position reconstruction method avoids traditional methods,such as bubble sorting and traversal searching,simplifies the design of the logic firmware,and reduces the time complexity from O(n2)to O(n).This study contributes to the advancement in measuring neutron beam flux for BNCT.

关键词： Position reconstruction FPGA Readout electronics Neutron flux spatial distribution

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Transgene directionally integrated into C-genome of Brassica napus

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Chinese Science Bulletin 2006年第13期51卷 1578-1585页

作者： LI jun fang Xiaoping wang Zhuan LUO Lixia HU Qiong Key Laboratory of Oil Crops Genetic Improvement of the Ministry of Agriculture Institute of Oil Crops Research Chinese Academy of Agricultural Sciences Wuhan 430062 China

Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus To obtain C-genome transgenic B. napus, herbi- cide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the trans- genic B. oleracea as paternal plants and 8 non- transgenic varieties of B. rapa as maternal plants, C- genome transgenic B. napus with bar gene was arti- ficially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resyn- thesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray con- firmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines.

关键词： C-染色体组 Bar基因定向转基因芸苔再合成

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Validity of Diagnostic Evidence for Deceased Cases in Hospitals

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Biomedical and Environmental Sciences 2008年第3期21卷 247-252页

作者： XIA WAN LI-jun wang jun-fang wang AI-PING CHEN GONG-HUAN YANG Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Peking Union Medical University Beijing 100005 China Chinese Center for Disease Control and Prevention Beijing 100050 China

Objective To determine the validity of the diagnostic evidence for deceased cases in hospitals. Methods All information collected from medical records of the deceased cases in tertiary care health facilities was input into ottr database. Four diagnosis levels were determined based on level of diagnostic evidence： level Ⅰ was based on autopsy, pathology or operative exploration, level Ⅱ on physical and laboratory tests plus expert clinical judgment, level Ⅲ on expert clinical judgment, level Ⅳ on postmortem assumptions. After the diagnostic evidence of each deceased case was reviewed by a panel of three experts, the diagnostic level of each diagnosis was determined. Results Among the 2102 medical cases for verbal autopsy study, only 26 （1.24%） afforded diagnostic evidence for level Ⅲ Among the level Ⅲ evidence-based cases of death, the major causes of death were cardiovascular diseases, respiratory diseases, and gastroenterological diseases. According to some special symptoms and medical histories, these cases could be diagnosed by comprehensive clinical judgment. Only one case met the criteria for level Ⅳ. Conclusion Level Ⅰ diagnostic evidence is hard to attain in China because of the traditional concept and economic restriction. The causes for 2101 deaths can be validated by level Ⅱ or Ⅲ diagnostic evidence.

关键词： Validity Diagnosis criteria Diagnostic evidence Laboratory examination

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Preparation of modified SiO_2 colloidal spheres with succinic acid and the assembly of colloidal crystals

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Chinese Science Bulletin 2007年第4期52卷 461-466页

作者： fang jun wang XiuFeng wang LieSong CHENG Bing WU Yuan-Ting ZHU WanLin School of Materials Science and Engineering Shaanxi University of Science and Technology Xi'an 712081 China

SiO2 colloidal spheres were synthesized by St-ber method. In order to enhance surface charge of the SiO2 spheres, they were modified with succinic acid. Scanning electron microscope (SEM) shows that the average size of modified SiO2 spheres is 473 nm, and its distribution standard deviation is less than 5%; Fourier-transform infrared spectra (FT-IR) and X-ray photoelectron spectrometer (XPS) results indicate that one end of succinic acid is chemically bonded to the SiO2 spheres through esterification; Zeta potential of the modified SiO2 spheres in water solution is improved from -53.72 to -67.46 mV, and surface charge density of the modified SiO2 spheres is enhanced from 0.19 to 0.94 μC/cm2. SiO2 colloidal crystal was fabricated from aqueous colloidal solution by the vertical deposition method at 40℃ and 60% relative humidity. SEM images show that the sample of SiO2 colloidal crystal is face-centered cubic (fcc) structure with its (111) planes parallel to the substrate. Transmission measurement shows the existence of photonic band gap at 1047 nm.

关键词：琥珀酸二氧化硅胶质球胶质晶体光学晶体制备表面电荷密度

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