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Antibacterial activity of sequentially extracted organic solvent extracts of fruits,flowers and leaves of Lawsonia inermis *** Jaffna

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 798-802页

作者： E Christy Jeyaseelan S Jenothiny MK Pathmanathan jp jeyadevan Department of Botany Faculty of ScienceUniversity of Jaffna Department of Chemistry Faculty of ScienceUniversity of Jaffna

Objective:To reveal the antibacterial activity of sequentially extracted different cold organic solvent extracts of fruits,flowers and leaves of Lawsonia inermis(L against)some pathogenic ***:Powders of fruits,flowers and leaves of L inermis were continuously extracted with dichloromethane(DCM),ethyl acetate and ethanol at ambient *** dried extracts were prepared into different concentrations and tested for antibacterial activity by agar well diffusion method,and also the extracts were tested to detennine the available ***:Except DCM extract of flower all other test extracts revealed inhibitory effect on all tested bacteria and their inhibitory effect differed significantly(P<0.05).The highest inhibitory effect was showed by ethyl acetate extract of flower against Staphylococcus aureus(***)and Pseudomonas aeruginosa(***),and ethyl acetate extract of fruit on Escherichia coli(***)and Bacillus subtilis(***).The ethyl acetate and ethanol extracts of flower,fruit and leaf expressed inhibition even at 1 mg/100μl against all test *** the tested phytochemicals flavonoids were detected in all test extracts except DCM extract of ***:The study demonstrated that the ethyl acetate and ethanol extracts of fruit and flower of L inermis are potentially better source of antibacterial agents compared to leaf extracts of respective solvents.

关键词： Lawsonia inermis Antibacterial activity Sequential extraction Well diffusion method

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