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A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

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BIOCELL 2021年第2期45卷 331-337页

作者： SERGIO GASTÓN CASPE joséluis konrad DADIN PRANDO MOORE JUAN MANUEL SALA LILIAN LISCHINSKY CARLOS MANUEL CAMPERO CLAUDIO GUSTAVO BARBEITO Laboratory of Pathology Experimental Station MercedesInstituto Nacional de Tecnología AgropecuariaCorrientes3470Argentina Laboratory of Theriogenology School of Veterinary SciencesUniversidad Nacional del NordesteCorrientesW3402BKGArgentina Animal Health Group Experimental Station BalcarceInstituto Nacional de Tecnología AgropecuariaBalcarceB7620EMAArgentina Laboratory of Histology and Embryology School of Veterinary SciencesUniversidad Nacional de La PlataLa Plata1900Argentina

Water buffalo(Bubalus bubalis)and domestic cattle(Bos taurus)are closely related ***,embryo transfer interspecies has been attempted without any *** failure in hybrid embryo-implantation is associated with the glycocode in the maternal-fetal *** patterns have been studied in different species of ruminants;however,in ***,only the binucleated cells(BNC)have been *** glycocode is essential for a successful embryo-implantation and can be defined by Lectin-Histochemistry(LHC).The aim of this study is to compare the glycosylation pattern of placenta and uterus in water buffaloes and cattle by *** samples of placenta and uterus from pregnant Mediterranean female water buffaloes(Buf1)and Angus cows(Bov1)were *** animals were euthanized at 98 days of *** was carried out using twelve lectins(Con A,LCA,PSA,sWGA,PHA-e,SBA,UEA-1,WGA,RCA-1,PNA,DBA,BSA-1).The intensity of lectin binding was semiquantitatively scored using a scale of 0(negative)to 3(strongly positive).Difference between species was found in trophoblast layer by PSA,SWGA,PNA and BSA-1,in BNC,and in the mononuclear cells by LCA,PSA,PHA-e,DBA,BSA-1,*** utero,differences in the apical cellular membrane and the secretion of glands were identified by DBA and RCA-1,and in the cytoplasm of those glandular epithelial cells by PHA-e,BSA-1,WGA,and *** both species,BNC presented a strong positive reaction with DBA and SBA,a moderate response by LCA,PHA-e,BSA-1 and PNA lectin,and a low reaction by PSA,UEA-1,SWGA,WGA,Con A and *** results found in this study suggest that although both species are closely related,glycosylation patterns in the placenta and uterus are different,thus providing a possible reason for embryo transfer not being possible between these species.

关键词： Lectin-histochemistry Water buffalo Placenta Glycosylation pattern

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Light correcting light with nonlinear optics

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Advanced Photonics 2024年第2期6卷 41-49页

作者： Sachleen Singh Bereneice Sephton Wagner Tavares Buono Vincenzo D'Ambrosio Thomas konrad Andrew Forbes University of the Witwatersrand School of Physics Università degli studi di Napoli “Federico Ⅱ” Dipartimento di Fisica “E.Pancini” University of Kwa Zulu-Natal School of Chemistry and Physics

Structured light, where complex optical fields are tailored in all their degrees of freedom, has become highly topical of late, advanced by a sophisticated toolkit comprising both linear and nonlinear *** undesired structure from light is far less developed, leveraging mostly on inverting the distortion,e.g., with adaptive optics or the inverse transmission matrix of a complex channel, both requiring that the distortion be fully characterized through appropriate measurement. We show that distortions in spatially structured light can be corrected through difference-frequency generation in a nonlinear crystal without any need for the distortion to be known. We demonstrate the versatility of our approach using a wide range of aberrations and structured light modes, including higher-order orbital angular momentum(OAM) beams,showing excellent recovery of the original undistorted field. To highlight the efficacy of this process, we deploy the system in a prepare-and-measure communications link with OAM, showing minimal cross talk even when the transmission channel is highly aberrated, and outline how the approach could be extended to alternative experimental modalities and nonlinear processes. Our demonstration of light-correcting light without the need for measurement opens an approach to measurement-free error correction for classical and quantum structured light, with direct applications in imaging, sensing, and communication.

关键词： phase conjugation aberration correction orbital angular momentum nonlinear optics

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The Silk roads: phylogeography of Central Asian dice snakes (Serpentes: Natricidae) shaped by rivers in deserts and mountain valleys

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Current Zoology 2024年第2期70卷 150-162页

作者： Daniel Jablonski konrad Mebert Rafaqat Masroor Evgeniy Simonov Oleg Kukushkin Timur Abduraupov Sylvia Hofmann Department of Zoology Comenius University in BratislavaBratislavaSlovakia Global Biology BirrSwitzerland Pakistan Museum of Natural History ShakarparianIslamabadPakistan Severtsov Institute of Ecology and Evolution Russian Academy of SciencesMoscowRussia T.I.Vyazemski Karadag Scientific Station-Nature Reserve—Branch of A.0.Kovalevsky Institute of Biology of the Southern Seas TheodosiaCrimea Zoological Institute of the RAS Saint PetersbourgRussia Institute of Zoology Academy of Sciences of the Republic of UzbekistanYunusabadTashkentUzbekistan Museum Koenig Bonn LIBLeibniz Institute for the Analysis of Biodiversity ChangeBonnGermany UFZ-Helmholtz Centre for Environmental Research Department of Conservation BiologyPermoserstrasse 1504318 LeipzigGermany

Influenced by rapid changes in climate and landscape features since the Miocene,widely distributed species provide suitable models to study the environmental impact on their evolution and current genetic *** dice snake Natrix tessellata,widely distributed in the Western Palearctic is one such *** aimed to resolve a detailed phylogeography of *** with a focus on the Central Asian clade with 4 and the Anatolia clade with 3 mitochondrial lineages,trace their origin,and correlate the environmental changes that affected their distribution through *** expected time of divergence of both clades began at 3.7 Mya in the Pliocene,reaching lineage differentiation approximately 1 million years *** genetic diversity in both clades is rich,suggesting different ancestral areas,glacial refugia,demographic changes,and colonization *** Caspian lineage is the most widespread lineage in Central Asia,distributed around the Caspian Sea and reaching the foothills of the Hindu Kush Mountains in Afghanistan,and Eastern European lowlands in the *** distribution is limited by deserts,moun-tains,and cold steppe ***,Kazakhstan and Uzbekistan lineages followed the Amu Darya and the Syr Darya water systems in Central Asia,with ranges delimited by the large Kyzylkum and Karakum *** the western side,there are several lineages within the Anatolia clade that converged in the central part of the peninsula with 2 being endemic to Western *** distribution of both main clades was affected by expansion from their Pleistocene glacial refugia around the Caspian Sea and in the valleys of Central Asia as well as by environmental changes,mostly through aridification.

关键词： biogeography colonization Eurasia genetic diversity mitochondrial DNA Paratethys refugia water snakes.

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Chronic effects of stretching on range of motion with consideration of potential moderating variables:A systematic review with meta-analysis

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Journal of Sport and Health Science 2024年第2期13卷 186-194页

作者： Andreas konrad Shahab Alizadeh Abdolhamid Daneshjoo Saman Hadjizadeh Anvar Andrew Graham Ali Zahiri Reza Goudini Chris Edwards Carina Scharf David George Behm Institute of Human Movement Science Sport and HealthGraz UniversityGraz A-8010Austria School of Human Kinetics and Recreation Memorial University of NewfoundlandSt.John’sNL A1C 5S7Canada Department of Sport Injuries and Corrective Exercises Faculty of Sport SciencesShahid Bahonar University of KermanKerman 76169-13439Iran

Background:It is well known that stretch training can induce prolonged increases in joint range of motion(ROM).However,to date more information is needed regarding which training variables might have greater influence on improvements in ***,the purpose of this metaanalysis was to investigate the effects of stretch training on ROM in healthy participants by considering potential moderating variables,such as stretching technique,intensity,duration,frequency,and muscles stretched,as well as sex-specific,age-specific,and/or trained state-specific adaptations to stretch ***:We searched through PubMed,Scopus,Web of Science,and SportDiscus to find eligible studies and,finally,assessed the results from 77 studies and 186 effect sizes by applying a random-effect ***,by applying a mixed-effect model,we performed the respective subgroup *** find potential relationships between stretch duration or age and effect sizes,we performed a ***:We found a significant overall effect,indicating that stretch training can increase ROM with a moderate effect compared to the controls(effect size=-1.002;Z=-12.074;95%confidence interval:-1.165 to-0.840;p<0.001;I^(2)=74.97).Subgroup analysis showed a significant difference between the stretching techniques(p=0.01)indicating that proprioceptive neuromuscular facilitation and static stretching produced greater ROM than did ballistic/dynamic ***,there was a significant effect between the sexes(p=0.04),indicating that females showed higher gains in ROM compared to ***,further moderating analysis showed no significant relation or ***:When the goal is to maximize ROM in the long term,proprioceptive neuromuscular facilitation or static stretching,rather than ballistic/dynamic stretching,should be *** to consider in future research as well as sports practice is that neither volume,intensity,nor frequency of stretching were

关键词： Flexibility Long-term stretching Stretch training

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德国南部钻石城太空送来的七万吨礼物

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环球人文地理 2024年第3期 96-103页

作者：袁莘捷 lebaer Wolfgang Ralf ArTo konrad filbert Andrey＿Lobachev Sina Ettmer flickr

钻石受到无数人追捧，其高昂的价格也让人望而却步。让人意外的是，在德国巴伐利亚州的西部山区，人们竟用七万吨钻石，建造了一座闪闪发光的钻石城。诺德林根是一座中世纪小镇，距今已有上千年历史。这里四面都有围墙，是德国目前仅有...

钻石受到无数人追捧，其高昂的价格也让人望而却步。让人意外的是，在德国巴伐利亚州的西部山区，人们竟用七万吨钻石，建造了一座闪闪发光的钻石城。诺德林根是一座中世纪小镇，距今已有上千年历史。这里四面都有围墙，是德国目前仅有的三座完全被城墙环绕的城市之一。小镇曾经安宁祥和，也曾经饱经硝烟，而许多建筑，甚至差点毁于一场大火。

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Advances in single-cell sequencing:insights from organ transplantation

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Military Medical Research 2022年第1期9卷 86-97页

作者： Ying Wang Jian-Ye Wang Angelika Schnieke konrad Fischer Ying Wang and Jian-Ye Wang have contributed equally to this review Chair of Livestock Biotechnology School of Life Sciences WeihenstephanTechnical University of MunichLiesel Beckman Str i85354 FreisingGermany

Single-cell RNA sequencing(scRNA-seq)is a comprehensive technical tool to analyze intracellular and intercellular interaction data by whole transcriptional profile ***,we describe the application in biomedical research,focusing on the immune system during organ transplantation and *** conventional transcriptome analysis,this method provides a full map of multiple cell populations in one specific tissue and presents a dynamic and transient unbiased method to explore the progression of allograft dysfunction,starting from the stress response to final graft *** promising sequencing technology remarkably improves individualized organ rejection treatment by identifying decisive cellular subgroups and cell-specific interactions.

关键词： Single-cell RNA sequencing Transplant rejection Immune cell interactions Transcriptional profiling 10×Genomics chromium

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论宪法规范力

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法治现代化研究 2024年第2期8卷 190-200页

作者： [德]康拉德·黑塞刘亚巍曾韬(译) 不详浙江省法制研究所吉林大学法学院德国科隆大学

“法律上的宪法”在德国一度被认为只不过是对现实权力的规范表达,宪法学作为一门规范科学的价值也因此受到挑战。在此背景下,本文指出,法律上的宪法与政治和社会现实之间有着相互制约的关系,宪法既受现实条件的制约,又规范和塑造着政... 详细信息

“法律上的宪法”在德国一度被认为只不过是对现实权力的规范表达,宪法学作为一门规范科学的价值也因此受到挑战。在此背景下,本文指出,法律上的宪法与政治和社会现实之间有着相互制约的关系,宪法既受现实条件的制约,又规范和塑造着政治和社会现实。宪法规范力的作用发挥,受制于现实条件的影响、宪法对社会变化的适应性、人们对宪法的尊崇意志、宪法内容以及宪法实践。紧急状态是检验宪法规范力的关键时刻,然而《德国基本法》并未做好准备以接受对其规范力的检验。原因之一是,从“人们对宪法的尊崇意志”这个宪法规范力赖以发挥作用的最可靠的保障来看,《德国基本法》只是有限地扎根于普遍意识并得到其支持;原因之二是,包括自由原则等在内的宪法内容受到了深刻的质疑;原因之三是,《德国基本法》缺少关于紧急状态权的规定。宪法规范力能否得到有效发挥,取决于宪法规范力及其基本前提是否得到恪守和强化。

关键词：宪法规范力法律上的宪法实际上的宪法《德国基本法》

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Fabrication of ultrafine-grained Ti-15Zr-xCu alloys through martensite decompositions under thermomechanical coupling conditions

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Journal of Materials Science & Technology 2022年第32期127卷 19-28页

作者： Wenwei Gao Hai Wang konrad Koenigsmann Shuyuan Zhang Ling Ren Ke Yang Shi-changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and Engineering University of Science and Technology of ChinaShenyang 110016China University of Chicago ChicagoIL 60637USA

Grain refinement is a well-recognized method to simultaneously increase the strength and ductility of metallic *** of ultrafine-grained metals in bulk using a simple low-cost approach is a long-term goal for material *** this work,based on the chemical composition of a biomedical Ti-15Zr alloy,a series of novel Ti-15Zr-xCu(x=0,3,5,7 wt.%)alloys were designed and *** alloys were quenched in the singleβphase region to obtain a martensitic microstructure and deformed in the temperature range of 710–750℃to obtain an ultrafine-grained microstructure through martensite decomposition under thermomechanical coupling *** results showed that Cu alloying could increase the dynamic recrystallization(DRX)nucleation rate due to its role in both refining martensitic lath width and increasing dislocation *** alloying could also suppress grain growth due to the precipitated Ti_(2)Cu particles exerting pinning forces on the grain *** optimal Cu content in the Ti-15Zr-xCu alloy was determined to be 5 wt.%.After being subjected to a compression leading to a 70%height reduction at 730℃and 1 s^(–1),the grain size of the Ti-15Zr-5Cu alloy was only 180±70 *** tensile strength of the as-prepared alloy reached 975±10 MPa,which was 45%higher than that of the conventional Ti-15Zr alloy(673±16 MPa).This increase in strength was achieved without any reduction in *** comprehensive mechanical properties of the ultrafinegrained Ti-15Zr-5Cu alloy are better than that of the Roxolid Ti–Zr alloy currently used for dental implants.

关键词： Titanium alloy Dynamic recrystallization Hot deformation Nucleation

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Topological protection of partially coherent light

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Photonics Research 2022年第5期10卷 1223-1231页

作者： konrad TSCHERNIG GABRIEL MARTINEZ-NICONOFF KURT BUSCH MIGUEL A.BANDRES ARMANDO PEREZ-LEIJA Max-Born-lnstitut 12489 BerlinGermany Humboldt-Universität zu Berlin Institut fur PhysikAG Theoretische Optik&Photonik12489 BerlinGermany Coordinacidn deÓptica Instituto Nacional de AstrofisicaÓpticay Electronica72840 TonantzintlaPueblaMexico CREOL The College of Optics and PhotonicsUniversity of Central FloridaOrlandoFlorida 32816USA

Topological physics exploits concepts from geometry and topology to implement systems capable of guiding waves in an unprecedented *** ideas have led to the development of photonic topological insulators,which are optical systems whose eigenspectral topology allows the creation of light states that propagate along the edge of the system without any coupling into the bulk or backscattering even in the presence of ***,topological protection is a fully coherent effect,and it is not clear to what extent topological effects endure when the wavefronts become partially ***,we study the interplay of topological protection and the degree of spatial coherence of classical light propagating in disordered photonic topological *** results reveal the existence of a well-defined spectral window in which partially coherent light is topologically *** opens up the design space to a wider selection of light sources,possibly yielding smaller,cheaper,and more robust devices based on the topological transport of light.

关键词： topological coherent topology

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Mitochondrial transport of catalytic RNAs and targeting of the organellar transcriptome in human cells

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Journal of Molecular Cell Biology 2023年第8期15卷 40-53页

作者： PawełGłodowicz konrad Kuczynski Romain Val AndréDietrich Katarzyna Rolle Department of Molecular Neurooncology Institute of Bioorganic ChemistryPolish Academy of Sciencesul.Z.Noskowskiego 12/1461-704 PoznanPoland Institute of Plant Molecular Biology French National Center for Scientific Research(CNRS)and University of Strasbourg12 rue du Général Zimmer67084 StrasbourgFrance

Mutations in the small genome present in mitochondria often result in severe *** genetic strategies have been explored,aiming to rescue such mutations.A number of these strategies were based on the capacity of human mitochondria to import RNAs from the cytosol and designed to repress the replication of the mutated genomes or to provide the organelles with wild-type versions of mutant ***,the mutant RNAs present in mitochondria turned out to be an obstacle to therapy and little attention has been devoted so far to their ***,we present the development of a strategy to knockdown mitochondrial RNAs in human cells using the transfer RNA-like structure of Brome mosaic virus or Tobacco mosaic virus as a shuttle to drive trans-cleaving ribozymes into the organelles in human cell *** obtained a specific knockdown of the targeted mitochondrial ATP6 mRNA,followed by a deep drop in ATP6 protein and a functional impairment of the oxidative phosphorylation *** strategy provides a powerful approach to eliminate mutant organellar transcripts and to analyse the control and communication of the human organellar genetic system.

关键词： human cells mitochondria ribozyme RNA trafficking transcriptome manipulation tRNA-like structure

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