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Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers

Horticultural Plant Journal 2020年第2期6卷 61-70页

作者： Rim Ouni Anna Zborowska Jasna Sehic Sarra Choulak jinaki hormaza Larisa Garkava-Gustavsson Messaoud Mars Research Unit on Agrobiodiversity(UR13AGR05) Higher Agronomic InstituteIRESA-University of SousseChott-Mariem 4042Tunisia Department of Plant Breeding Swedish University of Agricultural Sciences23053 AlnarpSweden Department of Plant Breeding-Balsgd Swedish University of Agricultural SciencesFjkestadsven 459SE-29194 KristianstadSweden Laboratory of Genetics Biodiversity and Bioresources Valorisation(LR11ES41)ISBMUniversity of MonastirMonastir 5000Tunisia Instituto de Hortofruticultura Subtropical Mediterránea La Mayora(IHSM-UMA-CSIC)Estaci on Experimental La MayoraAlgarrobo-CostaMálaga 29750Spain

Growing pear has a long tradition in Tunisia, and numerous local cultivars possessing an excellent adaptability and resilience potential to climatic variation are present. This large adaptability is associated with an important genetic diversity, which is threatened to *** measures have to be taken in order to properly evaluate and conserve this local material. Microsatellite markers were used to assess the level of genetic diversity among Tunisian pear germplasm, and compare it with some European varieties and wild pear species. 61 pear accessions representing eight groups(six groups from Tunisia, one from Northern Europe and another group composed of wild pear) have been genotyped using SSR markers derived from apple and pear. The pear accessions showed a significant polymorphism and 95 polymorphic alleles were found. The number of alleles per locus varied from 5 for CH04e03 locus to 14 for CH01d09 locus with an average of 9.4 alleles per ***, the mean gene diversity(H_e) per locus ranged from 0.192 to 0.752. Genetic distance values and cluster analyses revealed high genetic similarities among the Tunisian groups. Factorial correspondence analysis(FCA) categorized the accessions into three independent groups where Tunisian local accessions agglomerated together distantly from European and wild pear accessions. Additionally, UPGMA dendrogram grouped accessions into two clusters, confirmed thereafter by the Bayesian model-based Structure analysis. The results showed 16 putative triploid accessions found in the local germplasm. This study provides valuable information to develop strategies of local pear conservation and use.

关键词： Pyrus communis molecular marker genetic diversity local accession

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Self-compatibility in peach[Prunus persica(L.)Batsch]:patterns of diversity surrounding the S-locus and analysis of SFB alleles

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Horticulture Research 2020年第1期7卷 675-689页

作者： Donia Abdallah Ghada Baraket Veronica Perez Amel Salhi Hannachi Jose I.hormaza Author details 1Facultédes Sciences de Tunis Département BiologieUniversitéde Tunis El Manar2092 TunisTunisie Laboratorio de Agrobiología Juan JoséBravo Rodríguez(Cabildo Insular de La Palma) Unidad Técnica del Instituto de Productos Naturales y Agrobiología(IPNA-CSIC)38700 S/C La PalmaCanary IslandsSpain Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora(IHSM La Mayora-UMA-CSIC) 29750 Algarrobo-CostaMalagaSpain

Self-incompatibility(SI)to self-compatibility(SC)transition is one of the most frequent and prevalent evolutionary shifts in flowering *** L.(Rosaceae)is a genus of over 200 species most of which exhibit a Gametophytic SI ***[Prunus persica(L.)Batsch;2n=16]is one of the few exceptions in the genus known to be a fully selfcompatible ***,the evolutionary process of the complete and irreversible loss of SI in peach is not well understood and,in order to fill that gap,in this study 24 peach accessions were *** tube growth was controlled in self-pollinated flowers to verify their self-compatible *** linkage disequilibrium association between alleles at the S-locus and linked markers at the end of the sixth linkage group was not significant(P>0.05),except with the closest markers suggesting the absence of a signature of negative frequency dependent selection at the *** of SFB1 and SFB2 protein sequences allowed identifying the absence of some variable and hypervariable domains and the presence of additionalα-helices at the *** and evolutionary analysis of SFB nucleotide sequences showed a signature of purifying selection in SFB2,while the SFB1 seemed to evolve ***,our results show that the SFB2 allele diversified after *** and ***(almond)divergence,a period which is characterized by an important bottleneck,while SFB1 diversified at a transition time between the bottleneck and population expansion.

关键词： compatibility linkage alleles

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Conductivity of the phloem in mango (Mangifera indica L.)

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Horticulture Research 2021年第1期8卷 2045-2053页

作者： Miguel Barcelo-Anguiano Jose I.hormaza Juan M.Losada Instituto de Hortofruticultura Subtropical y Mediterranea La Mayora(IHSM La Mayora-CSIC-UMA) Avda Dr.Wienberg s/n.29750Algarrobo-CostaMalagaSpain

Mango(Mangifera indica L.,Anacardiaceae),the fifth most consumed fruit worldwide,is one of the most important fruit crops in tropical regions,but its vascular anatomy is quite *** studies examined the xylem structure in the stems of mango,but the anatomy of the phloem has remained elusive,leaving the long-distance transport of photoassimilates *** combined fluorescence and electron microscopy to evaluate the structure of the phloem tissue in the tapering branches of mango trees,and used this information to describe the hydraulic conductivity of its sieve tube elements following current models of fluid transport in *** revealed that the anatomy of the phloem changes from current year branches,where it was protected by pericyclic fibres,to older ones,where the lack of fibres was concomitant with laticiferous canals embedded in the phloem *** was present in the sieve plates,but also in the walls of the phloem sieve cells,making them discernible from other phloem cells.A scaling geometry of the sieve tube elements-including the number of sieve areas and the pore size across tapering branches-resulted in an exponential conductivity towards the base of the *** evaluations in mangofit with previous measurements of the phloem architecture in the stems of forest trees,suggesting that,despite agronomic management,the phloem sieve cells scale with the tapering *** pipe model theory applied to the continuous tubing system of the phloem appears as a good approach to understand the hydraulic transport of photoassimilates in fruit trees.

关键词： transport anatomy walls

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利用DNA标记进行温带果树品种鉴定和指纹分析

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国外作物育种 2003年第2期22卷 62-67页

作者： A.Wunsch J.I.hormaza 邱敦莲

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