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REGULATION OF ALFALFA LEGHEMOGLOBIN SYNTHESIS AT TRANSLATIONAL LEVEL

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Science in China,Ser.B 1986年第1期29卷 50-56页

作者：荆玉祥 Institute of Botany Academia Sinica Beijing

A comparative study on translational activity of alfalfa leghemoglobin mRNAs from different age nodules indicated that the activity of the mRNAs for leghemoglobin synthesis was not affected by the age of *** antisera against alfalfa leghemoglobins or their antileghemoglobin IgGs could immunologically react to pea leghemoglobin, but not to soybean leghemoglobins. In in vitro translation systems, when alfalfa leghemoglobins were added to their own and pea leghemoglobin mRNAs, both the translations were inhibited. When soybean leghemoglobins, bovine hemoglobins, bovine serum albmnin were added to the systems, the synthesis of alfalfa leghemoglobins were not affected, Hemin could enhance the synthesis of leghemoglobins. The experiments showed that the leghemoglobin synthesis is automatically regulated at the translational level.

关键词： REGULATION OF ALFALFA LEGHEMOGLOBIN SYNTHESIS AT TRANSLATIONAL LEVEL mRNA AT

Present status and development on biological nitrogen fixation research in China

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Chinese Science Bulletin 2003年第10期48卷 954-960页

作者： SHEN Shihua & jing yuxiang Key Laboratory of Photosynthesis and Plant-Environment MolecularPhysiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China Key Laboratory of Photosynthesis and Plant-Environment Molecular Physiology Institute of Botany Chinese Academy of Sciences Beijing 100093 China

This presentation introduces the advances inbiological nitrogen fixation research abroad, in particular,describes the great progress and achievements on itsresearch in China as follows: collection of rhizobial resources and establishment of the largest database of Rhizobium inChina, correction and development of Rhizobium taxonomy in international; discovery of a couple of nif genes,identification and unification of linkage among the nif gene operons of Klebsiella pneumoniae, finding of regulative mechanism of positive regulation nif gene and its sensitivity to oxygen, temperature; finding of the activity of nodulation gene nodD3 product in Sinorhizobium meliloti which is notcontrolled by flavonoid produced from its host alfalfa;finding of the association between expression of genes coding the products for carbon utilization and nitrogen metabolism and their regulations; chemical synthesis of nodulationfactor of Sinorhizobium meliloti; constructions of engineered nitrogen fixers and utilization in practice based on theresearch of gene expression and regulation; chemicalsimulation of the structure and function of nitrogenase and bringing forward the model of nitrogenase active center for the first time in international and synthesis of modelcompounds which were paid attention by colleagues abroad. Finally, the development of nitrogen fixation research inChina in future has been put forward, suggesting that the nifgene regulation and its role in providing crops with nitrogen element, signal transduction and molecular interactions between Rhizobium and legume, coupling between carbonand nitrogen metabolisms, nitrogen fixation andphotosynthesis, and functional genomics of nitrogen-fixing nodule symbiosis, etc., would be actively worked on.

关键词：现状发展生物固氮技术中国固氮酶基因表达微生物植物相互作用化学模型

Quantitative lithium substitution of carboxyl hydrogens in polyacrylic acid binder enables robust SiO electrodes with durable lithium storage stability

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Journal of Energy Chemistry 2024年第10期97卷 352-360,I0007页

作者： Weihua Wang Wenyi Li Siyi jing Huiping Yang Huiqun Wang Ling Huang yuxiang Mao Xikun Pang Yudai Huang Li Zhang State Key Laboratory of Physical Chemistry of Solid Surfaces Department of Chemical and Biochemical EngineeringCollege of Chemistry and Chemical EngineeringCollaborative Innovation Centre of Chemistry for Energy Materials(iChEM)Xiamen UniversityXiamen 361005FujianChina State Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources College of ChemistryXinjiang UniversityUrumqi 830017XinjiangChina

The design of advanced binders plays a critical role in stabilizing the cycling performance of large-volume-effect silicon monoxide(SiO)*** the classic polyacrylic acid(PAA)binder,the self-association of-COOH groups in PAA leads to the formation of intramolecular and intermolecular hydrogen bonds,greatly weakening the bonding force of the binder to SiO ***,strengthening the binder-material interaction from the perspective of binder molecular regulation poses a significant ***,a modified PAA-Li_(x)(0.25≤x≤1)binder with prominent mechanical properties and adhesion strength is specifically synthesized for SiO anodes by quantitatively substituting the carboxylic hydrogen with *** appropriate lithium substitution(x=0.25)not only effectively increases the number of hydrogen bonds between the PAA binder and SiO surface owing to charge repulsion effect between ions,but also guarantees moderate entanglement between PAA-Li_x molecular chains through the ion-dipole *** such,the PAA-Li_(0.25)/SiO electrode exhibits exceptional mechanical properties and the lowest volume change,as well as the optimum cycling(1237.3 mA h g^(-1)after 100cycles at 0.1 C)and rate performance(1000.6 mA h g^(-1)at 1 C),significantly outperforming the electrode using pristine PAA *** work paves the way for quantitative regulation of binders at the molecular level.

关键词： Polyacrylic acid binder SiO anode Quantitative lithium substitution Charge repulsion effect Adhesion strength

High-Efficiency Rectifier for Wireless Energy Harvesting Based on Double Branch Structure

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Journal of Donghua University(English Edition) 2023年第4期40卷 438-445页

作者：崔恒荣焦劲华杨威荆玉香 College of Information Science and Technology Donghua UniversityShanghai 201620China

A rectifier circuit for wireless energy harvesting(WEH) with a wide input power range is proposed in this paper. We build up accurate models of the diodes to improve the accuracy of the design of the rectifier. Due to the nonlinear characteristics of the diodes, a new band-stop structure is introduced to reduce the imaginary part impedance and suppress harmonics. A novel structure with double branches and an optimized λ/4 microstrip line is proposed to realize the power division ratio adjustment by the input power automatically. The proposed two branches can satisfy the two cases with input power of-20 dBm to 0 dBm and 0 dBm to 15 dBm, respectively. Here, dBm = 10 log(P mW), and P represents power. An impedance compression network(ICN) is correspondingly designed to maintain the input impedance stability over the wide input power range. A rectifier that works at 2.45 GHz is implemented. The measured results show that the highest efficiency can reach 51.5% at the output power of 0 dBm and higher than 40% at the input power of-5 dBm to 12 dBm.

关键词： diode modeling rectifier circuit impedance compression network power division strategy

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生物固氮和非豆科植物固氮根瘤工程问题

科技导报 1987年第2期 56-61页

作者：荆玉祥中国科学院植物研究所固氮研究室副研究员

一、生物固氮及其研究的意义所谓生物固氮,是指自然界中一些微生物和蓝藻将大气中的氮转化成氨的过程,其反应式是N2+3H2→2NH3。目前能固氮的生物细菌有35属,藻类有21属,除了豆科植物等以外,种种其他微生物大约共达300多种。研究生物固... 详细信息

一、生物固氮及其研究的意义所谓生物固氮,是指自然界中一些微生物和蓝藻将大气中的氮转化成氨的过程,其反应式是N2+3H2→2NH3。目前能固氮的生物细菌有35属,藻类有21属,除了豆科植物等以外,种种其他微生物大约共达300多种。研究生物固氮,在理论和实际应用上都很重要。大家知道,氮素是组成生命的基本元素之一。很多生物分子都有氮元素,特别是由氨基酸组成的蛋白质,氮是基本成分。氮素又是植物生长的主要肥料,然而占空气大约80％成分的氮素

关键词：根瘤菌根毛侵染固氮酶共生性根瘤细菌固氮菌还原酶生物固氮结瘤根瘤豆科植物

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高温辐照对混凝土结构性能损伤影响研究

工业建筑 2022年第11期52卷 133-138页

作者：荣华荆玉翔王誉林耿岩中冶建筑研究总院有限公司北京100088 中冶检测认证有限公司北京100088 中国广核集团有限公司广东深圳518038

为了确保核电站在延寿后安全可靠的运行,核电站的钢筋混凝土结构在长期辐照下的性能表现非常重要。建立了高温辐照混凝土力学性能退化的理论模型。通过GSC模型和Mori-Tanaka模型来描述混凝土的多相和多尺度内部结构及其力学和传输性能... 详细信息

为了确保核电站在延寿后安全可靠的运行,核电站的钢筋混凝土结构在长期辐照下的性能表现非常重要。建立了高温辐照混凝土力学性能退化的理论模型。通过GSC模型和Mori-Tanaka模型来描述混凝土的多相和多尺度内部结构及其力学和传输性能。使用复合损伤力学方法估算了辐照和高温对混凝土力学性能的影响。基于温度梯度和辐照引起的损伤,使用多组扩散方程计算了损伤混凝土的传输特性变化。最后,建立了辐照-热-力耦合机制作用下混凝土的长期性能表现的分析方法。建立的分析框架可以进一步与商用计算软件相结合,应用于核电站钢筋混凝土结构的服役性能动态评价中。

关键词：核电站辐照混凝土力学性能损伤传输性能

Proteomic analysis of rice seedlings under abiotic stresses and in the de-etiolation process

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Proteomic analysis of rice seedlings under abiotic stresses ...

Proteomic analysis of the response of Liangyoupeijiu (a super high-yield hybrid rice) seedlings to cold stress

首届全国蛋白质组学学术研讨会

作者： Xiaojuan Li, Yang Ping-fang, Yu Liang, yuxiang jing, Shihua Shen Key Laboratory of Photosynthesis and Environmental Molecular PhysiologyInstitute of Botany, CAS, Beijing, P. R. China, 100093

Abiotic stresses, e.g. cold, salt, and drought, are main limiting factors of plant growth and crop yield. Three-day-old seedlings of rice (Oryza sativa L indica) cultivar 9311 were submitted to cultural medium containing 250 mM NaCl (Salt treatment), 25% polyethylene glycol (PEG) (Osmotic treatment), 50 μM ABA and 10℃for 48 hours (Cold treatment), respectively. Proteins were extracted from shoots of seedlings and isolated by two-dimensional electrophoresis (2D-E). More than 800 reproducible protein spots were detected, and 121, 104, and 118 protein spots were found up- or down- regulated in the salt, osmotic, and cold treatment, respectively. Mass spectrometry analysis and database searching helped to identify 25 proteins. These proteins are involved in regulation of photosynthesis and carbohydrate, energy metabolisms, reactive oxygen species scavenging, molecular chaperone systems, and protein synthesis. The crosstalk between stresses and stress-specific proteomic changes were *** is also an important environmental factor to plants. In this presentation, proteomic changes of rice seedlings moving from dark to light were introduced. Seeds of rice (O. sativa L indicd) cultivar 9311 were germinated and grown in dark for two weeks. The seedlings were illuminated with white light for 0, 6, 12 and 24 hrs, respectively. Total proteins of the second leaves from bottom to top were extracted and applied to two-dimensional electrophoresis (2-DE) in parallel. 621, 594, 577, and 529 protein spots were detected on the 2-D gel of 0, 6, 12 and 24 hr light treatment, respectively. 97 proteins changed in the process, among which 70 were down regulated and 27 were up regulated. The functions of the identified proteins were discussed.

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Proteomic analysis of the response of Liangyoupeijiu (a supe...

基于粒子群优化和卷积神经网络的电力系统运行状态辨识

首届全国蛋白质组学学术研讨会

作者： Yang Pingfang, jing yuxiang, Shen Shihua, Kuang Tingyun, Key laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China Graduate College of Chinese Academy of Sciences, Beijing, China

Liangyoupeijiu is a super high-yield hybrid rice bred recently. In spite of its advantage in yield, it is sensitive to cold, which constrains its popularization. We performed a 4℃cold treatment on Liangyoupeijiu seedlings, then extracted the leaf proteins. After 2-dimensional gel electrophoresis (2-DE) separation and Matrix assisted Laser Desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, a series of differentially displayed proteins were identified. Some metabolism-associated proteins were found among the down-regulated proteins, such as caramoyl phosphate synthetase, transketolase 1, dihydrolipoamide dehydrogenase and glyceraldehyde 3-phosphate dehydrogenase. As for the up-regulated proteins, they include FtsH-like protein plastid fusion and/or translocation factor (Pftf) and actin that are negative for rice growth, although stress resistant proteins such as nucleoside diphosphate kinase I were identified. Our results indicate that cold may inhibit Liangyoupeijiu growth through decreasing metabolism activity and damaging cell structure.

关键词： Oryza sativa Hybrid rice Cold stress Proteome

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电网技术 2024年第1期48卷 315-324页

作者：杨晶赵津蔓孟润泉张东霞李柏堉武宇翔电力系统运行与控制山西省重点实验室(太原理工大学) 山西省太原市030024 中国电力科学研究院有限公司北京市海淀区100192

随着“双碳”目标的提出,高比例可再生能源和高比例电力电子设备正成为电力系统发展的重要趋势和关键特征,其间歇性、不确定性使电力系统实时运行状态辨识面临严峻挑战。为此,该文提出一种基于粒子群优化和卷积神经网络(particle swarm ... 详细信息

随着“双碳”目标的提出,高比例可再生能源和高比例电力电子设备正成为电力系统发展的重要趋势和关键特征,其间歇性、不确定性使电力系统实时运行状态辨识面临严峻挑战。为此,该文提出一种基于粒子群优化和卷积神经网络(particle swarm optimization and convolutional neural network,PSO-CNN)的高精度电力系统实时运行状态辨识方法。首先,该方法同时考虑电力系统安全域与稳定域下的暂态问题,适用于暂态稳定故障前、故障中及故障后多场景的电力系统运行状态辨识。其次,为确保样本数据中新能源机组出力方式的全面性,采用拉丁超立方抽样方法对精细化仿真数据采样,考虑到实际电力系统中存在状态类别极端不平衡问题,引入PSO算法调节模型不同类别损失函数权重以提高模型对极端不均衡样本的辨识效果。最后,分别在IEEE39节点系统及某省级电网系统中对所提方法进行评估,实验结果证明了所提状态辨识方法的有效性及鲁棒性。

关键词：电力系统运行状态辨识粒子群优化算法深度学习