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Expression Optimization and Characterization of the Catalytic Domain of Human MT3-MMP

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Chemical Research in Chinese Universities 2006年第2期22卷 129-133页

作者： SHI xiu-juan jin Feng-hai WANG Hui-ling yang jin-gang WANG Zhi-yong FANG Xue-xun Key Laboratory of Molecular Enzymology and Engineering the Ministry of Education School of Pharmacy College of Life Science Jilin University Changchun 130021 P. R. China

Matrix metalloproteinases（MMPs） are a family of proteases that are required for many biological processes and are also elevated in many pathological conditions. MMP inhibitors （MMPIs） may therefore be useful as therapeutic agents in treating a number of diseases including cancer, cardiovascular diseases and arthritis. Attempts have been made to develop MMPIs. Recombinant MMPs have been used to screening MMPs in vitro assays. In this work, we report the expression of MMP-16 in E. coli and the characterization of the recombinant MMP-16 with a commonly used MMP substrate DQ-gelatin.

关键词： Matrix metalloproteinase(MMP) Protein expression Catalytic domain MMP-16

A Novel Chemical Oscillator with Tyrosine as the Substrate

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Chinese Chemical Letters 2003年第12期14卷 1281-1284页

作者： Wu yang Jie REN jin Zhang GAO Hua yang Dong Yu Lv xiu Hui LIU Institute of Chemistry Northwest Normal UniversityLanzhou 730070

A new chemical oscillating reaction system involving the manganese(II)-catalyzed reaction among potassium bromate, acetone and tyrosine in acidic medium was described in this paper. The apparent activation energy of the induction period (Ein) and that of oscillation period (Ep) were calculated, Ein=110.61KJ/mol, Ep=159.41KJ/mol. The optimum concentration of each reactant was investigated and a steady chemical oscillating system was obtained.

关键词： Chemical oscillator tyrosine substrate mechanism.

High production of fatty alcohols in Yarrowia lipolytica by coordination with glycolysis

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Science China Chemistry 2019年第8期62卷 1007-1016页

作者： jin-Lai Zhang Ying-xiu Cao yang-Zi Peng Cong-Cong jin Qiu-Yan Bai Ruo-Si Zhang Duo Liu Ying-jin Yuan Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education) Tianjin University Tianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) School of Chemical Engineering and Technology Tianjin UniversityTianjin 300072China

Fatty alcohol biosynthesis by oleaginous microbes was a promising alternative to the petroleum or other non-renewable resources-based process. However, low titer and yield hamper the further industrial and commercial applications. Here, we developed an efficient strategy to coordinate fatty alcohol with glycolysis which achieved a ‘pull-and-push’ effect to improve fatty alcohol production. Transcript profiling indicated that genes in carbohydrate metabolism were up-regulated significantly in response to high fatty alcohol production. Based on it, 11 glycolysis promoters were screening to express fatty acyl-CoA reductase(FAR) to relate the fatty alcohol production with the up-regulated carbohydrate metabolism, and the fatty alcohol production reached 557 mg/L when FAR was expressed by the promoter of PFBAin. RNA-seq and qRT-PCR analysis demonstrated that a ‘pull-and-push’ effect caused by the coordination system dynamically enhanced the product synthesis flux from top to bottom, which was also testified and intensified by doubled glucose concentration. After manipulating structural and regulatory genes of lipid metabolism, the final strain achieved up to 5.75 g/L fatty alcohol production from modified YPD medium(containing 91 g/L glucose) in shake flasks, which represented the highest titer reported to date. This work offered a feasible and effective reference for dynamic manipulation of fatty acid-derived chemicals synthesis.

关键词： fatty alcohol biosynthesis coordinative expression Yarrowia lipolytica fatty acyl-CoA reductase(FAR) metabolic engineering

Dust-Acoustic Soliton in a Streaming Plasma

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Chinese Physics Letters 1997年第6期14卷 432-435页

作者： LIU jin-yuan MA jin-xiu Department of Modern Physics University of Science and Technology of ChinaHefei 230027

Large amplitude dust-acoustic solitons in a plasma with ion streaming are investigated on a very slow time scale of dust motion with variable grain *** is found that both compressional and rarefactive solitons can coe... 详细信息

关键词： solitons soliton rare

Surface-Enhanced Raman Spectroscopy:Principles,Methods,and Applications in Energy Systems

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Chinese Journal of Chemistry 2023年第3期41卷 355-369页

作者： Quan-Feng He Yu-jin Zhang Zhi-Lan yang jin-Chao Dong xiu-Mei Lin Jian-Feng Li State Key Laboratory of Physical Chemistry of Solid Surfaces College of Chemistry and Chemical EngineeringCollege of EnergyiChEMXiamen UniversityXiamenFujian361005 China Innovation Laboratory for Sciences and Technologies of Energy Materials of Fujian Province(IKKEM) XiamenFujian361005 China Department of Chemistry and Environment Science Fujian Province University Key Laboratory of Analytical ScienceMinnan Normal UniversityZhangzhouFujian363000 China Shenzhen Research Institute of Xiamen University ShenzhenGuangdong518000 China

Surface-enhanced Raman spectroscopy(SERS)has advanced significantly since its *** experimental and theoretical efforts have been made to understand the SERS effect and demonstrate its *** to its extremely high sensitivity and selectivity and ability to provide molecular fingerprint information,SERS has a wide range of applications in surface and interfacial chemistry,energy,materials,biomedicine,environmental analysis,*** review aims to provide readers with an understanding of the principles,methodologies,and applications of *** briefly introduce the fundamental theory of the SERS enhancement mechanism and summarize the details of the preparation of SERS-active *** applications of SERS in energy systems are then highlighted,including probing surface reactions and interfacial charge transfer of batteries and ***,the challenges and prospects of SERS research are discussed.

关键词： Surface-enhance Raman spectroscopy In situ characterization Li-ion batteries Water splitting Fuel cells

Upstream ion wave excitation in an ion-beam–plasma system

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Chinese Physics B 2018年第5期27卷 349-354页

作者： Kai-yang Yi jin-xiu Ma Zi-An Wei Zheng-Yuan Li Department of modern Physics and Chinese Academy of Sciences Key Laboratory of Geospace Environment University of Science and Technology of ChinaHefei 230026China

Plasma normal modes in ion-beam–plasma systems were experimentally investigated previously only for the waves propagating in the downstream(along the beam)*** this paper,the ion wave excitation and propagation in the upstream(against the beam)direction in an ion-beam–plasma system were experimentally studied in a double plasma *** waves were launched by applying a ramp voltage to a negatively biased excitation *** kinds of wave signals were detected,one is a particle signal composed of burst ions and the other is an ion-acoustic signal arising from the background *** signals were identified by the dependence of the signal velocities on the characteristics of the ramp *** velocity of the burst ion signal increases with the decrease of the rise time and the increase of the peak-to-peak amplitude of the applied ramp voltage while that of the ion-acoustic signal is independent of these *** adjusting these parameters such that the burst ion velocity approaches to the ion-acoustic velocity,the wave–particle interaction can be observed.

关键词： ion-beam-plasma system upstream direction ion-acoustic wave burst ion

ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis

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Journal of Integrative Plant Biology 2017年第9期59卷 680-692页

作者： Ke-jin yang Lei Guo xiu-Li Hou Hua-Qin Gong Chun-Ming Liu Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China University of Chinese Academy of Sciences Beijing 100049China School of Life Science and Technology Nanyang Normal UniversityNanyang 473061China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China

In sexual organisms, division of the zygote initiates a new life cycle. Although several genes involved in zygote division are known in plants, how the zygote is activated to start embryogenesis has remained elusive. Here, we showed that a mutation in ZYGOTE-ARREST 3 （ZYG3） in Arabidopsis led to a tight zygote-lethal phenotype. Map-based cloning revealed that ZYG3 encodes the transfer RNA （tRNA） ligase AtRNL, which is a single-copy gene in the Arabidopsis genome. Expression analyses showed that AtRNL is expressed throughout zygotic embryogenesis, and in meristematic tissues. Using pAtRNL：：cAtRNL-sYFP- complemented zyg3/zyg3 plants, we showed that AtRNL is localized exclusively in the cytoplasm, suggesting that tRNA splicing occurs primarily in the cytoplasm. Analyses using partially rescued embryos showed that mutation in AtRNL compromised splicing of intron-containing tRNA. Mutations of two tRNA endonuclease genes, SEN1 and SEN2, also led to a zygote-lethal phenotype. These results together suggest that tRNA splicing is critical for initiating zygote division in Arabidopsis.

关键词： NL ZYGOTE-ARREST 3 that encodes the tRNA ligase is essential for zygote division in Arabidopsis GUS Figure

Magnetic flux participation in solar surface magnetism during solar cycle 24

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Research in Astronomy and Astrophysics 2019年第5期19卷 61-74页

作者： Chun-Lan jin jing-xiu Wang Key Laboratory of Solar Activity National Astronomical Observatories Chinese Academy of Sciences

This study aims at investigating surface magnetic flux participation among different types of magnetic features during solar cycle 24. State-of-the-art observations from SDO/HMI and Hinode/SOT are combined to form a unique database in the interval from April 2010 to October 2015. Unlike previous studies, the statistics presented in this paper are feature-detection-based. More than 20 million magnetic features with relatively large scale, such as sunspot/pore, enhanced and quiet networks, are automatically detected and categorized from HMI observations, and the internetwork features are identified from SOT/SP observations. The total flux from these magnetic features reaches 5.9×10^(22) Mx during solar minimum and2.4 × 10^(23) Mx in solar maximum. Flux occupation from the sunspot/pore region is 29% in solar *** and quiet networks contribute 18% and 21% flux during the solar minimum, and 50% and 9% flux in the solar maximum respectively. The internetwork field contributes over 55% of flux in the solar minimum, and its flux contribution exceeds that of sunspot/pore features in the solar maximum. During the solar active condition, the sunspot field increases its area but keeps constant flux density of about 150 G,while the enhanced network follows the sunspot number variation showing increasing flux density and area,but the quiet network displays decreasing area and somewhat increasing flux density of about 6%. The origin of the quiet network is not known exactly, but is suggestive of representing the interplay between mean-field and local dynamos. The source, magnitude and possible importance of ‘hidden flux' are discussed in some detail.

关键词： Sun:magnetic fields Sun:dynamo Sun:photosphere sunspots

Additive Maps Preserving Similarity of Operators on Banach Spaces

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Acta Mathematica Sinica,English Series 2006年第1期22卷 179-186页

作者： jin Chuan HOU xiu Ling ZHANG Department of Mathematics Shanxi Teachers University Linfen 041004 P R. China

Let X be an infinite-dimensional complex Banach space and denote by B（X） the algebra of all bounded linear operators acting on X. It is shown that a surjective additive map Φ from B（X） onto itself preserves similarity in both directions if and only if there exist a scalar c, a bounded invertible linear or conjugate linear operator A and a similarity invariant additive functional ψ on B（X） such that either Φ（T） = cATA^-1 ＋ ψ（T）I for all T, or Φ（T） = cAT＊A^-1 ＋ ψ（T）I for all T. In the case where X has infinite multiplicity, in particular, when X is an infinite-dimensional Hilbert space, the above similarity invariant additive functional ψ is always zero.

关键词： Additive maps on operator algebras Similarity of operators Banach spaces