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CagA^+ *** Induces Akt1 Phosphorylation and Inhibits Transcription of p21^(WAF1/CIP1) and p27^(KIP1) via PI3K/Akt1 Pathway

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Biomedical and Environmental Sciences 2010年第4期23卷 273-278页

作者： SHU-PING LI XUE-JUN CHEN AI-HUA SUN jin-fang zhao JIE YAN State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital of Zhejiang University Hangzhou 310003 Zhejiang China Department of Clinical Laboratory The Children's Hospital of Zhejiang University Hangzhou 310003 Zhejiang China Faculty of Basic Medicine Zhejiang Medical College Hangzhou 310053 Zhejiang China Department of Medical Microbiology and Parasitology Medical College of Zhejiang University Hangzhou 310058 Zhejiang China

Objective Cytotoxin-associated protein （CagA） of H. pylori has been confirmed to be closely associated with gastric inflammation and tumorigenesis, but the mechanism behind it is little understood. In this study, we try to determine roles of CagA＋ strain in activating PI3K/Akt1 signaling pathway, and affecting expression of p21WAF1/CIP1 and p27KIP1, and also in releasing IL-8 in host cells. Methods Akt1 phosphorylation and IL-8 levels of CagA＋ and CagAˉ strain infected AGS cells were detected by ELISAs. Two quantitative RT-PCRs were established to measure p21WAF1/CIP1 and p27KIP1 mRNA levels in the CagA＋ and CagAˉ strain infected cells. LY294002, an inhibitor of PI3K/Akt pathway, was used to define effect of the pathway in IL-8 release. Results CagA＋ strain could induce an obvious elevation of Akt1 phosphorylation in the infected AGS cells while CagAˉ strain failed to do so. The CagA＋ H. pylori strain infected AGS cells showed significant drops both in p21WAF1/CIP1 and p27KIP1 mRNA levels, whereas the CagAˉ H. pylori strain caused a remarkable increase in p21WAF1/CIP1 mRNA without affecting p27KIP1 gene transcription in the AGS cells. Both the CagA＋ and CagAˉ H. pylori strains enabled AGS cells to produce close elevated levels of IL-8, and the LY294002 block resulted in unexpected elevations of IL-8 levels. Conclusion CagA can activate PI3K/Akt1 pathway that plays an inhibitory role in IL-8 release in H. pylori infected AGS cells. Activation of PI3K/Akt1 pathway and subsequent negative regulation of p21^WAF1/CIP1 and p27^KIP1 expression might be involved in CagA-associated carcinogenesis.

关键词： Helicobater pylori CagA PI3K Akt1 p21^WAF1/CIP1 p27^KIP1 IL-8

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Comparing the precision of the pSOFA and SIRS scores in predicting sepsis-related deaths among hospitalized children:a multi-center retrospective cohort study

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World Journal of Emergency Medicine 2022年第4期13卷 259-265页

作者： Chun zhao Mei-yun Xin jing Li jin-fang zhao Yu-juan Wang Wei Wang Qian Gao Jie Chen Qi-wei Wang You-peng jin Department of Pediatric Intensive Care Unit Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250021China Department of Pediatrics Affiliated Hospital of Jining Medical UniversityJining 272129China Department of Pediatrics Qingdao Women and Children HospitalQingdao 266034China Department of Pediatric Intensive Care Unit Shandong Provincial HospitalCheeloo College of MedicineShandong UniversityJinan 250021China

BACKGROUND:The latest sepsis definition includes both infection and organ failure,as evidenced by the sequential organ failure assessment(SOFA)***,the applicability of the pediatric SOFA score(pSOFA)is not yet *** study evaluated the effectiveness of both pSOFA and system inflammatory reaction syndrome(SIRS)scores in predicting sepsis-related pediatric ***:This is a retrospective multi-center cohort study including hospitalized patients<18 years old with diagnosed or not-yet-diagnosed *** analyses were carried out to evaluate risk factors for in-hospital *** to Youden index(YI),three sub-categories of pSOFA were screened out and a new simplified pSOFA score(spSOFA)was *** effectiveness and accuracy of prediction of pSOFA,SIRS and spSOFA was retrieved from the area under the receiver operating characteristic curve(AUROC)and Delong’s ***:A total of 1,092 participants were eligible for this study,and carried a 23.4%in-hospital mortality *** 24-h elevated pSOFA score(24 h-pSOFA),bloodstream infection,and mechanical ventilation(MV)requirement were major risk factors associated with sepsis-related *** AUROC analysis confirmed that the spSOFA provided good predictive capability in sepsis-related pediatric deaths,relative to the 24 h-pSOFA and ***:The pSOFA score performed better than SIRS in diagnosing infected children with high mortality ***,it is both costly and ***,therefore,proposed spSOFA to accurately predict patient outcome,without the ***,additional investigations,involving a large sample population,are warranted to confirm the conclusion of this study.

关键词： Simple-pSOFA pSOFA Children Mortality Infection SIRS

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临床学习型组织文化与护士核心能力的相关性调查

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中华医院管理杂志 2012年第4期28卷 280-283页

作者：祝筠吕芳芳封秀萍靳昭芳赵娟陈海萍山东大学附属省立医院济南250012 山东大学护理学院

目的了解临床学习型组织文化与护士核心能力的相关性，为进行学习型组织建设的医院提高护士核心能力提供理论依据和实施建议。方法应用一般资料调查表、注册护士核心能力量表和护理学习型组织量表对济南市3所三级医院的680名护士进行问... 详细信息

目的了解临床学习型组织文化与护士核心能力的相关性，为进行学习型组织建设的医院提高护士核心能力提供理论依据和实施建议。方法应用一般资料调查表、注册护士核心能力量表和护理学习型组织量表对济南市3所三级医院的680名护士进行问卷调查。结果学习型组织总分及4个层面与护士核心能力呈正相关（P〈0．01），学习型组织中的“全局意识与战略领导”层面对护士核心能力各层面均有显著影响力（P〈0．05），学习型组织中的“团队合作与创新”、“倡导对话交流和调查研究”层面对护士核心能力的部分层面有显著影响力（P〈0．05）。结论医院在进行学习型组织建设过程中，应培养护士的全局意识，为学习提供战略性的领导，加强合作与创新，促进探讨与交流，以提高护士核心能力，进而提升护理队伍的整体水平。

关键词：护士核心能力学习型组织调查研究

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Single-cell transcriptomic profiling reveals ZEB1-mediated regulation in microglial subtypes and the impact of exercise on neuroinflammatory responses

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Traditional Medicine Research 2025年第2期10卷 55-64页

作者： jin-fang Wu Yi-Sheng Chen Yu-Chun Xie Department of Rehabilitation Medicine Jiangsu Province Geriatric HospitalNanjing 210024China Fujian Key Laboratory of Toxicant and Drug Toxicology Medical CollegeNingde Normal UniversityNingde 352100China Central Laboratory Jiangsu Province Geriatric HospitalNanjing 210024China

Background:This study aims to identify distinct cellular subtypes within brain tissue using single-cell transcriptomic analysis,focusing on specific biomarkers that differentiate cell types and the effects of traditional and exercise ***:Four samples were analyzed:older control(OC),older exercise(OE),younger control(YC),and younger exercise(YE).Single-cell RNA sequencing was used to distinguish cellular subtypes through their biomarker *** visualization included violin and t-SNE plots to illustrate biomarker expression across cell clusters such as oligodendrocytes,microglia,and ***,BV2 cells were exposed to amyloid-beta fragments to simulate Alzheimer’s disease,assessing the impact of exercise-induced cellular ***:Distinct cellular subtypes were identified:oligodendrocytes(MBP,St18),microglia(Dock8),and astrocytes(Aqp4,Gpc5).Sample OE was predominantly oligodendrocytes,while YE had more astrocytes,inhibitory neurons,and Canal-Retzius *** showed a significant presence of Olfm3+ganglion ***1 gene knockout revealed changes in SMAD family gene expression,which regulate *** stress levels were also ***:This profiling enhances our understanding of brain cellular functions and interactions,potentially informing targeted therapies in neurological *** may influence brain cell immune responses and cell death pathways by regulating specific gene expressions,offering new insights for treating neuroinflammation and degeneration.

关键词： single-cell transcriptomics brain tissue cellular subtypes biomarker expression neuronal diversity

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A full-length 3D structure for MAPK/ERK kinase 1（MEKl）

A full-length 3D structure for MAPK/ERK kinase 1（MEKl）

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hao LIANG,Tao LIU~*,fang-jin CHEN,zhao-Qing LIU,Shao-Jun LIU~* State Key Laboratory of Proteomics,Department of Neurobiology,Institute of Basic Medical Sciences,Beijing 100850, China

＜正＞Objective The MAPK/ERK kinases（MEK1 and MEK2） were thought to function as multifarious funnels transmitting various upstream signals to downstream *** to the technological limitations,the full length 3D structures of MEKs are unavailable,and thus the detailed molecular mechanisms remain *** present study is to build the full length 3D structure of MEK1 and study its molecular dynamics *** The structure was constructed by homology modeling and studied by molecular dynamics ***（1） Get the full length 3D structure of MEK1.（2） The N-terminal part,the proline-rich region and the C-terminal part are flexible.（3） The N-terminal long helix is always close to the remained part of MEK1.（4） The N-terminal 8 amino acids is mainly maintained between the N-terminal long helix and the main part of the *** Get the full length 3D structure of MEK1 and its molecular dynamics characteristics.

关键词： MEK1 homology modeling molecular dynamics simulation 3D structure

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Highly mass activity electrocatalysts with ultralow Pt loading on carbon black for hydrogen evolution reaction

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 182-190页

作者： Shaorou Ke Yajing zhao Xin Min Yanghong Li Ruiyu Mi Yangai Liu Xiaowen Wu Minghao fang zhaohui Huang Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid WastersNational Laboratory of Mineral MaterialsSchool of Materials Science and TechnologyChina University of Geosciences(Beijing)Beijing 100083China

Pt-based nanocatalysts offer excellent prospects for various ***,the low loading of Pt with excellent performance for efficient and stable nanocatalysts still presents a considerable *** this study,nanocatalysts with ultralow Pt content,excellent performance,and carbon black as support were prepared through in-situ ***~2-nm particles uniformly and stably dispersed on carbon black because of the strong s-p-d orbital hybridizations between carbon black and Pt,which suppressed the agglomeration of Pt *** unique structure is beneficial for the hydrogen evolution *** catalysts exhibited remarkable catalytic activity for hydrogen evolution reaction,exhibiting a potential of 100 mV at 100 mA·cm^(-2),which is comparable to those of commercial Pt/C *** activity(1.61 A/mg)was four times that of a commercial Pt/C catalyst(0.37 A/mg).The ultralow Pt loading(6.84wt%)paves the way for the development of next-generation electrocatalysts.

关键词： hydrogen evolution reaction ultralow platinum in-situ synthesis ultrasound

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Unlocking the stable interface in aqueous zinc-ion battery with multifunctional xylose-based electrolyte additives

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Journal of Energy Chemistry 2025年第1期100卷 770-778页

作者： Xiaoqin Li Jian Xiang Lu Qiu Xiaohan Chen Yinkun zhao Yujue Wang Qu Yue Taotao Gao Wenlong Liu Dan Xiao zhaoyu jin Panpan Li Institute for Advanced Study Chengdu University School of Mechanical Engineering Chengdu University College of Food and Biological Engineering Chengdu University Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China College of Materials Science and Engineering Sichuan University

The growth of dendrites and the side reactions occurring at the Zn anode pose significant challenges to the commercialization of aqueous Zn-ion batteries（AZIBs）. These challenges arise from the inherent conflict between mass transfer and electrochemical kinetics. In this study, we propose the use of a multifunctional electrolyte additive based on the xylose（Xylo） molecule to address these issues by modulating the solvation structure and electrode/electrolyte interface, thereby stabilizing the Zn anode. The introduction of the additive alters the solvation structure, creating steric hindrance that impedes charge transfer and then reduces electrochemical kinetics. Furthermore, in-situ analyses demonstrate that the reconstructed electrode/electrolyte interface facilitates stable and rapid Zn2+ion migration and suppresses corrosion and hydrogen evolution reactions. As a result, symmetric cells incorporating the Xylo additive exhibit significantly enhanced reversibility during the Zn plating/stripping process, with an impressively long lifespan of up to 1986 h, compared to cells using pure ZnSO4electrolyte. When combined with a polyaniline cathode, the full cells demonstrate improved capacity and long-term cyclic stability. This work offers an effective direction for improving the stability of Zn anode via electrolyte design, as well as highperformance AZIBs.

关键词： Solvation

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中文版护理人员学习型组织量表的信效度检验

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护理学杂志 2011年第17期26卷 13-16页

作者：吕芳芳祝筠陈海萍赵娟靳昭芳山东大学护理学院山东济南250012 山东大学附属省立医院

目的为护理人员学习型组织文化提供可行的测评工具。方法以方便抽样方法选取513名临床护理人员,采用中文版学习型组织量表进行问卷调查,通过计算量表的内部一致性系数、探索性因素分析和相关性分析来评价量表的信度、效度和维度构成。... 详细信息

目的为护理人员学习型组织文化提供可行的测评工具。方法以方便抽样方法选取513名临床护理人员,采用中文版学习型组织量表进行问卷调查,通过计算量表的内部一致性系数、探索性因素分析和相关性分析来评价量表的信度、效度和维度构成。结果护理人员学习型组织量表包括全局意识与战略领导、创造学习环境以形成共同愿景、团队合作与创新、倡导对话交流和调查研究4个维度,共计16个测量指标;累积方差贡献率为69.115%,量表内部一致性系数Cronbach′sα为0.887。各因子与总分的相关系数为0.604～0.765。结论护理人员学习型组织量表在信度和效度上均达到了可接受的标准,且符合心理测量学的要求,在护理领域具有适用性。

关键词：护理人员学习型组织学习型组织量表信度效度

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济南市三级医院护理人员学习型组织现状的调查

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解放军护理杂志 2012年第16期29卷 12-14,18页

作者：祝筠吕芳芳赵娟靳昭芳陈海萍山东大学附属省立医院门诊部山东济南250021 山东大学护理学院山东济南250012 山东大学附属省立医院血液科山东济南250021 山东大学附属省立医院肿瘤科山东济南250021 山东大学附属省立医院心内科山东济南250021

目的调查济南市部分三级医院护理人员学习型组织的现状及人口学变量对其的影响,为护理领域学习型组织的构建提供参考依据。方法应用一般资料调查表和中文版学习型组织量表对济南市5所三级医院的560名临床护理人员进行问卷调查。结果共... 详细信息

目的调查济南市部分三级医院护理人员学习型组织的现状及人口学变量对其的影响,为护理领域学习型组织的构建提供参考依据。方法应用一般资料调查表和中文版学习型组织量表对济南市5所三级医院的560名临床护理人员进行问卷调查。结果共回收有效问卷513份。513名临床护士学习型组织的总均分为(70.42±6.56)分,条目均分为4.40分,其中团队合作与创新的条目得分最高,倡导对话交流和调查研究得分最低;不同年龄、学历的护士在学习型组织文化感知上差异有统计学意义(P<0.05)。结论济南市部分三级医院护理人员学习型组织建设处于中等水平,年轻、较高学历水平的护士具有提升学习型组织文化的强烈愿望;在护理人员学习型组织建设中,可根据其主要构成因素及人口学特征制定针对性的干预策略。

关键词：护理三级医院管理学习型组织

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济南市三级医院护士核心能力的现状调查

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中华护理教育 2011年第9期8卷 390-393页

作者：吕芳芳祝筠靳昭芳赵娟陈海萍山东大学护理学院济南市250012 山东大学附属省立医院

目的调查济南市三级医院注册护士核心能力现状,分析人口学变量对护士核心能力的影响,为护士培训提供依据。方法应用一般资料调查表和注册护士核心能力量表,对济南市5所三级医院的513名护士进行问卷调查。结果护士核心能力总均分为（2.79... 详细信息

目的调查济南市三级医院注册护士核心能力现状,分析人口学变量对护士核心能力的影响,为护士培训提供依据。方法应用一般资料调查表和注册护士核心能力量表,对济南市5所三级医院的513名护士进行问卷调查。结果护士核心能力总均分为（2.79±0.58）分。在各个维度中,评判性思维和科研得分最低,伦理和法律实践得分最高。工作年限、职称、工作满意度、婚姻状况、自评健康状况是其主要影响因素。结论济南市三级医院护士核心能力处于中等偏上水平,在护士培训中需注重评判性思维和科研能力的培养,并加强对年轻、未婚护士的培养,以提升护士的核心能力。

关键词：护士核心能力问卷调查

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