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Neuroanatomy and morphological diversity of brain cells from adult crayfish Cherax quadricarinatus

Journal of Oceanology and Limnology 2018年第6期36卷 2368-2378页

作者： DUAN Hu jin songjun LI Fuhua ZHANG Xiaojun XIANG Jianhai Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciences Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and Technology

As in vertebrates, brains play key roles in rhythmic regulation, neuronal maintenance, diff erentiation and function, and control of the release of hormones in arthropods. But the structure and functional domains of the brain are still not very clear in crustaceans. In the present study, we reveal the structural details of the brain in the redclaw crayfish using hematoxylin-eosin staining and microscopic examination, firstly. The brain of crayfish is consist of three main parts, namely, protocerebrum, deutocerebrum, and tritocerebrum, including some tracts and commissures, briefly. Secondly, at least 9 kinds of brain cells were identified on the basis of topology and cell shapes, as well as antibody labeling. We also provide morphological details of most cell types, which were previously un-described. In general, four types of glia and three types of neurosecretory cells were described except cluster 9/11 and cluster 10 cells. Glia were categorized into another three main kinds:(1) surface glia;(2) cortex glia; and(3) neuropile glia in addition to astrocytes identified by GFAP labelling. And neurosecretory cells were categorized into I, Ⅱ and III types based on morphological observation. Finally, cluster 9/11 and 10 cells derived from the brain of crayfish, could be used for primary culture about 7–9 d under the optimized conditions. There results provide a resource for improving the knowledge of the still incompletely defined neuroendocrinology of this species. Using the crayfish as an animal model, we are easy to carry out further research in manipulating their endocrine system, exploring cellular and synaptic mechanisms so much as larval production on a small scale, such as in a cell or tissue.

关键词： Cherax quadricarinatus brain’s structure brain cells primary culture

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红螯螯虾XO-SG神经细胞的原代培养

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水产学报 2016年第10期40卷 1606-1612页

作者：张岩段虎金松君李富花相建海中国科学院海洋研究所实验海洋生物学重点实验室

本研究采用酶解的方法获得红螯螯虾眼柄XO-SG复合体的单个神经细胞,并依据显微观察对XO-SG神经细胞进行分类,同时利用Leibovitz’s L-15等作为基础培养基离体培养解离的神经细胞。目的是建立虾类XO-SG神经细胞的分类标准并确定合适的培... 详细信息

本研究采用酶解的方法获得红螯螯虾眼柄XO-SG复合体的单个神经细胞,并依据显微观察对XO-SG神经细胞进行分类,同时利用Leibovitz’s L-15等作为基础培养基离体培养解离的神经细胞。目的是建立虾类XO-SG神经细胞的分类标准并确定合适的培养条件,便于在体内外开展神经内分泌系统的调控研究。结果显示,根据神经细胞形态特点,红螯螯虾XO-SG神经内分泌细胞分为6种类型,不同类型的细胞在细胞大小以及显微结构上存在明显差异;建立了红螯螯虾眼柄XO-SG神经元的体外原代培养方法,细胞在改良的L-15培养基中存活状态良好,原代培养的神经细胞可以在体外存活14 d。离体培养过程中,不同类型神经细胞的再生速率存在差异,部分细胞在第2天就出现再生的轴突或树突。再生过程基本可以持续7 d,随后细胞开始萎缩凋亡。本研究为红螯螯虾眼柄神经细胞的分类以及利用神经细胞在体外开展虾类的内分泌代谢和调节机制研究提供了基础依据和研究平台。

关键词：红螯螯虾眼柄原代培养神经细胞再生

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Polarographic Studies on Associations of Midecamycin and Chloroquine with Hydrogen Peroxide

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Chemical Research in Chinese Universities 2005年第1期21卷 32-35页

作者： LINing songjun-feng XUMao-tian ZHANGYa InstituteofAnalyticalScience NorthwestUniversityXi＇an710069P.R.China DepartmentofChemistry SuideTeachers′SchoolSuide718000P.R.China

The associations of each of midecamycin and chloroquine phosphate to hydrogen peroxide were studied by using linear-potential scan polarography. In a 0.15 mol/L KH 2PO 4-NaOH(pH 7.4) buffer, the association ratio of the association complex of midecamycin to hydrogen peroxide is 1∶1, and the apparent association constant is 7.18. While in a 0.04 mol/L NH 3·H 2O-NH 4Cl(pH 9.5) buffer, the association ratio and the apparent association constant of the association complex of chloroquine phosphate to hydrogen peroxide are 1∶1 and 45.4, respectively. The formation of the association complexes stabilizes H 2O 2 and results in the accumulation of H 2O 2, which is baneful to human body.

关键词： Midecamycin Chloroquine phosphate Hydrogen peroxide Association Linear-potential scan polarography Reactive oxygen species

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A Generalized Funk's Section Theorem for Measures

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Wuhan University Journal of Natural Sciences 2017年第4期22卷 313-317页

作者： Lü songjun School of Mathematical Science Chongqing Normal University Chongqing 401331 China

If L is a star body in Rn whose central（n-i）-slices have the same（n-i）-dimensional measure μn-1（with appropriate density） as the central（n-i）-slices of an origin-symmetric star body K, then the corresponding n-dimensional measures μn of K and L satisfy μn（K）≤μn（L）. This extends a generalized Funk＇s section theorem for volumes to that for measures.

关键词： star body intersection body Funk's section theorem Radon transform

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Aluminum nanocrystals evolving from cluster to metallic state:Size tunability and spectral evidence

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Nano Research 2022年第2期15卷 838-844页

作者： Hao Wu Xiaoyu Cheng Hongguang Dong songjun Xie Sailing He National Engineering Research Centre for Optical Instruments College of Optical Science and EngineeringZhejiang UniversityHangzhou 310058China Ningbo Research Iristitute Ningbo 315100China

Sub-3 nm aluminum(Al)nanocrystal is an emerging class of nanomaterial with properties distinct to noble metal *** complete solution synthesis of aluminum nanoclusters was recently reported,and their photoluminescence(PL)was observed for the first *** the moment,there exists no method to tune the size of ultrasmall aluminum nanocrystals in solution thus no knowledge on the boundary state between aluminum nanoclusters to plasmonic *** this work,it is demonstrated a study of size-controlled solution synthesis of ultrasmall aluminum nanocrystals with size controlled between -2.2 and -3.8 *** the size results in three sets of spectral responses:(1)absorption due to nascent plasmons generated at -340 nm for larger particles,confirmed by Mie theory calculations;(2)significant decreased quantum yield of PL from -7.8%to -2.4%,indicating reduced quantum confinement effects and increased metallicity;(3)drop of fluorescence lifetime was observed,especially when the diameter of aluminum nanoparticles was changed from -3.0 to -3.8 *** study provides experimental evidence and insights to the transitional state between aluminum nanoclusters and plasmonic nanoparticles,which seems to occur at size larger than gold nanoclusters.

关键词： aluminum nanocrystals plasmon nanoclustors photoluminescence

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Flexible fuel engine based on multi-combustion control technologies

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Chinese Science Bulletin 2005年第2期50卷 185-189页

作者： LIXiaolu HUANGZhen QIAOXinqi songjun FANGJunhua XIAHuimin SchoolofMechanicalandPowerEngineering ShanghaiJiaotongUni-versityShanghai200030China NantongHaishiMarineMachinesCo. Nantong226003China

A combustion control strategy is proposed for diesel engine to reduce PM and NOx emissions significantly, which adopts some technologies including internal exhaust gas recirculation (EGR), split spray, adjustable fuel delivery advance angle and the application of alternative fuels. Based on this strategy, a flexible fuel engine has been developed. The experimental results show that this engine can be fueled with diesel fuel, alcohol, dimethyl carbonate (DMC), etc. It works with extremely low levels of particulate matter (PM) and NOx, 2~3% higher effective thermal efficiency on moderate and high loads when alternative fuels are used. This engine not only has lower exhaust emissions, but also can be fueled with those alternative fuels, which are difficult to be ignited by compression.

关键词：柴油机柔性燃料发动机多重燃烧控制技术作用燃料燃烧表征热效应

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Study on Disturbances and Its Effects on Nature Reserves

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Chinese Journal of Population,Resources and Environment 2006年第3期4卷 11-20页

作者： Zhang Linying Zhou Yongzhang Xu songjun College of Geographical Science South China Normal University Guangzhou 510631 China Center of Earth Environment and Resources Sun Yat-sen University Guangzhou 510275 China

As a widespread natural phenomenon, disturbances are considered a discrete event occurring in a natural ecosystem within various spatial and temporal scales. The present paper discusses the types of disturbances and their influence on the ecologic system in nature reserves. It is shown that nature reserves are facing a great challenge dealing with these disturbances. A rational control for disturbances should be improved in order to promote the healthy development of nature reserves this could be done by enhancing the publicity and training of environment protection, coupling management with communities around and other nature reserves, effective management methods, rational planning, and supporting nature servers.

关键词： disturbance nature reserve ecologic system sustainable development

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In vivo optical bioimaging by using Nd-doped LaF3 luminescent nanorods in the second near-infrared window

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Journal of Rare Earths 2019年第9期37卷 931-936,I0002页

作者： Shouyan Cheng Liu Liu Qiuhua Yang Youbin Li songjun Zeng Synergetic Innovation Center for Quantum Effects and Application Key Laboratory of Low-dimensional Quantum Structures and Quantum Control of Ministry of EducationSchool of Physics and ElectronicsHunan Normal University

Fluorescent imaging in the second near-infrared(NIR-Ⅱ,1000-1700 nm)region has attracted enormous research interest due to its advantages of unprecedentedly improved imaging sensitivity and spatial ***,high quality NIR-Ⅱemissive LaF3:Nd3+nanorods(NRs)with hexagonal phase structure and controlled size were synthesized by a hydrothermal *** the Nd^3+doping induced NIRⅡluminescent intensity evolution was studied,In vivo NIR-Ⅱbioimaging and real-time tracking reveal that these LaF3:Nd^3+NRs are mainly accumulated in liver and ***,in vivo toxicity assessment demonstrates that LaF3:Nd^3+NRs feature low biotoxicity and good biocompatibility in living ***,the NIR-Ⅱemitting hexagonal phase LaF3 NRs with controlled size are promising probes for optical bioimaging.

关键词： NIR-Ⅱimaging Real-time tracking Downshifting luminescence Rare earths

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Deep Residual Joint Transfer Strategy for Cross-Condition Fault Diagnosis of Rolling Bearings

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Journal of Dynamics, Monitoring and Diagnostics 2023年第1期2卷 42-51页

作者： songjun Han Zhipeng Feng School of Mechanical Engineering University of Science and Technology BeijingBeijing 100083China

Rolling bearings are key components of the drivetrain in wind turbines,and their health is critical to wind turbine *** practical diagnosis tasks,the vibration signal is usually interspersed with many disturbing components,and the variation of operating conditions leads to unbalanced data distribution among different *** intelligent diagnosis methods based on deep learning have been intensively studied,it is still challenging to diagnose rolling bearing faults with small amounts of *** address the above issue,we introduce the deep residual joint transfer strategy method for the cross-condition fault diagnosis of rolling ***-dimensional vibration signals are pre-processed by overlapping feature extraction techniques to fully extract fault *** deep residual network is trained in training tasks with sufficient samples,for fault pattern ***,three transfer strategies are used to explore the generalizability and adaptability of the pre-trained models to the data distribution in target *** them,the feature transferability between different tasks is explored by model transfer,and it is validated that minimizing data differences of tasks through a dual-stream adaptation structure helps to enhance generalization of the models to the target *** the experiments of rolling bearing faults with unbalanced data conditions,localized faults of motor bearings and planet bearings are successfully identified,and good fault classification results are achieved,which provide guidance for the cross-condition fault diagnosis of rolling bearings with small amounts of training data.

关键词： fault diagnosis feature transferability rolling bearing transfer strategy wind turbine

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One-pot hydrothermal fabrication of α-Fe_2O_3@C nanocomposites for electrochemical energy storage

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Journal of Energy Chemistry 2019年第1期28卷 1-8页

作者： Maiyong Zhu Jiarui Kan Jianmei Pan Wenjie Tong Qi Chen Jiacheng Wang songjun Li Institute of Polymer Materials School of Materials Science & Engineering Jiangsu University Department of Materials Science & Engineering Jingjiang College of Jiangsu University

A facile hydrothermal method was developed for the preparation of Fe_2O_3@C nanocomposites using FeCl_3·6H_2O as iron source and glucose as carbon source under alkaline condition. The morphology and structure of the as-prepared product were identified by transmission electron microscopy(TEM), high resolution transmission electron microscopy(HRTEM), field-emission scanning electron microscopy(FESEM),X-ray diffraction(XRD), Raman spectroscopy, Fourier Transform infrared spectroscopy(FTIR), and thermogravimetric analysis(TGA). The as-prepare α-Fe_2O_3@C nanocomposites were employed for supercapacitor electrode material. The synergistic combination of carbon electrical double-layer capacitance and α-Fe_2O_3 pseudo-capacitance established such nanocomposites as versatile platform for high performance supercapacitors. The synthesis method developed here is expected to obtain other metal oxide/carbon composite.

关键词： α-Fe2O3 Carbon Hydrothermal Pseudocapacitor

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