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Effect of soil archaea on N2O emission in alpine permafrost

Research in Cold and Arid Regions 2024年第2期16卷 45-62页

作者： YuZheng Gu ChenJie Dong ShengYun Chen jingwei jin PeiZhi Yang JianWei Chen PeiJie Wei Ali Bahadur College of Grassland Agriculture Northwest A&F University Cryosphere and Eco-Environment Research Station of Shule River Headwaters Key Laboratory of Cryospheric Science and Frozen Soil EngineeringNorthwest Institute of Eco-Environment and ResourcesChinese Academy of Sciences College of Ecology Lanzhou University BGI-Qingdao BGI-Shenzhen University of Chinese Academy of Sciences

Soil microbial communities are pivotal in permafrost biogeochemical cycles, yet the variations of abundant and rare microbial taxa and their impacts on greenhouse gas emissions in different seasons, remain elusive, especially in the case of soil archaea. Here, we conducted a study on soil abundant and rare archaeal taxa during the growing and non-growing seasons in the active layer of alpine permafrost in the Qinghai-Tibetan Plateau. The results suggested that, for the archaeal communities in the sub-layer, abundant taxa exhibited higher diversity, while rare taxa maintained a more stable composition from the growing to non-growing season. Water soluble organic carbon and soil porosity were the most significant environmental variables affecting the compositions of abundant and rare taxa, respectively. Stochastic and deterministic processes dominated the assemblies of rare and abundant taxa, respectively. The archaeal ecological network influenced N2O flux through different modules. Rare taxa performed an essential role in stabilizing the network and exerting important effects on N2O flux. Our study provides a pioneering and comprehensive investigation aimed at unravelling the mechanisms by which archaea or other microorganisms influence greenhouse gas emissions in the alpine permafrost.

关键词： Alpine permafrost Abundant and rare archaea Assembly processes Co-occurrence network N 2 O flux

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Five new terpenoids from Viburnum odoratissimum var.sessiliflorum

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Chinese Journal of Natural Medicines 2023年第4期21卷 298-307页

作者： LI Yang JIAN Yajiao XU Fan LUO Yongxin LI Zhixuan OU Yi WEN Yan jin jingwei ZHANG Chuarui GAN Lishe School of Biotechnology and Health Sciences International Healthcare Innovation InstituteWuyi UniversityJiangmen 529020China Chongqing Key Laboratory of Natural Product Synthesis and Drug Research School of Pharmaceutical SciencesChongqing UniversityChongqing 401331China Shandong Laboratory of Yantai Drug Discovery Bohai Rim Advanced Research Institute for Drug DiscoveryYantai 264117China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310058China

Five new terpenoids,including two vibsane-type diterpenoids(1,2)and three iridoid allosides(3-5),together with eight known ones,were isolated from the leaves and twigs of Viburnum odoratissimum var.sessiliflorum.Their planar structures and relative configurations were determined by spectroscopic methods,especially 2D NMR techniques.The sugar moieties of the iridoids were confirmed asβ-D-allose by GC analysis after acid hydrolysis and acetylation.The absolute configurations of neovibsanin Q(1)and dehydrovibsanol B(2)were determined by quantum chemical calculation of their theoretical electronic circular dichroism(ECD)spectra and Rh2(OCOCF_(3))_(4)-induced ECD analysis.The anti-inflammatory activities of compounds 1,3,4,and 5 were evaluated using an LPS-induced RA W264.7 cell model.Compounds 3 suppressed the release of NO in a dose-dependent manner,with an IC_(50) value of 55.64μmol·L^(-1).The cytotoxicities of compounds 1-5 on HCT-116 cells were assessed and the results showed that compounds 2 and 3 exhibited moderate inhibitory activities with IC_(50) values of 13.8 and 12.3μmol·L^(-1),respectively.

关键词： Viburnum odoratissimum var.sessilifolrum Terpenoids Acid hydrolysis ECD calculation Anti-inflammation

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MDP25 mediates the fine-tuning of microtubule organization in response to salt stress

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Journal of Integrative Plant Biology 2022年第6期64卷 1181-1195页

作者： Peizhi Yang jingwei jin jingru Zhang Dan Wang Xuechun Bai Wenfei Xie Tianming Hu Xuan Zhao Tonglin Mao Tao Qin College of Grassland Agriculture Northwest A&F UniversityYangling 712100China Research Center of Chinese Jujube Hebei Agricultural UniversityBaoding 071001China College of Horticulture Hebei Agricultural UniversityBaoding 071001China State Key Laboratory of Plant Physiology and Biochemistry Department of Plant SciencesCollege of Biological SciencesChina Agricultural UniversityBeijing 100193China

Microtubules are dynamic cytoskeleton structures playing fundamental roles in plant responses to salt stress.The precise mechanisms by which microtubule organization is regulated under salt stress are largely unknown.Here,we report that Arabidopsis thaliana MICROTUBULE-DESTABILIZING PROTEIN 25(MDP25;also known as PLASMA MEMBRANEASSOCIATED CATION-BINDING PROTEIN 1(PCaP1))helps regulate microtubule organization.Under salt treatment,elevated cytosolic Ca^(2+) concentration caused MDP25 to partially dissociate from the plasma membrane,promoting microtubule depolymerization.When Ca^(2+) signaling was blocked by BAPTA-AM or LaCl_(3),microtubule depolymerization in wild-type and MDP25-overexpressing cells was slower,while there was no obvious change in mdp25 cells.Knockout of MDP25 improved microtubule reassembly and was conducive to microtubule integrity under long-term salt treatment and microtubule recovery after salt stress.Moreover,mdp25 seedlings exhibited a higher survival rate under salt stress.The presence microtubule-disrupting reagent oryzalin or microtubule-stabilizing reagent paclitaxel differentially affected the survival rates of different genotypes under salt stress.MDP25 promoted microtubule instability by affecting the catastrophe and rescue frequencies,shrinkage rate and time in pause phase at the microtubule plus-end and the depolymerization rate at the microtubule minus-end.These findings reveal a role for MDP25 in regulating microtubule organization under salt treatment by affecting microtubule dynamics.

关键词： calcium MDP25 microtubule salt stress

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132份甘薯地方种质遗传多样性和群体结构分析

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中国农学通报 2024年第18期40卷 22-30页

作者：陈伊航唐朝臣张荣姚祝芳金晶炜王章英西北农林科技大学草业与草原学院陕西杨陵712100 广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室广州510640

本研究旨在评估中国甘薯地方种质资源的遗传多样性和群体结构。研究以132份甘薯地方种质为材料,对其表型性状、类胡萝卜素含量以及SSR分子标记进行遗传多样性分析和群体结构分析。分析结果表明,132份甘薯地方种质表型性状的平均变异系数... 详细信息

本研究旨在评估中国甘薯地方种质资源的遗传多样性和群体结构。研究以132份甘薯地方种质为材料,对其表型性状、类胡萝卜素含量以及SSR分子标记进行遗传多样性分析和群体结构分析。分析结果表明,132份甘薯地方种质表型性状的平均变异系数为45.84%,类胡萝卜素含量平均为6.33μg/g,最高为59.90μg/g,SSR分子标记的Nei’s多样性指数平均为0.15、Shannon’s多样性指数平均为0.23,表明存在一定程度的遗传多态性。进一步的群体结构分析表明,这132份甘薯地方种质可分为5个亚群。分子变异分析(AMOVA)显示,84%的变异存在于群体内,16%的变异存在于群体间。综上所述,中国的甘薯地方种质具有丰富的遗传多样性,且种质之间的遗传变异主要集中在亚群内部。这对于中国甘薯地方种资源的保护和新品种的培育提供了重要的信息。

关键词：甘薯表型性状 SSR分子标记类胡萝卜素含量遗传多样性群体结构地方种质

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Interface Engineering of Titanium Nitride Nanotube Composites for Excellent Microwave Absorption at Elevated Temperature

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Nano-Micro Letters 2024年第9期16卷 147-160页

作者： Cuiping Li Dan Li Shuai Zhang Long Ma Lei Zhang* jingwei Zhang Chunhong Gong College of Chemistry and Molecular Sciences Henan UniversityKaifeng 475004People’s Republic of China National and Local Joint Engineering Research Center for Applied Technology of Hybrid Nanomaterials Henan UniversityKaifeng 475004People’s Republic of China

Currently,the microwave absorbers usually suffer dreadful electromagnetic wave absorption(EMWA)performance damping at elevated temperature due to impedance mismatching induced by increased conduction loss.Consequently,the development of high-performance EMWA materials with good impedance matching and strong loss ability in wide temperature spectrum has emerged as a top priority.Herein,due to the high melting point,good electrical conductivity,excellent environmental stability,EM coupling effect,and abundant interfaces of titanium nitride(TiN)nanotubes,they were designed based on the controlling kinetic diffusion procedure and Ostwald ripening process.Benefiting from boosted heterogeneous interfaces between TiN nanotubes and polydimethylsiloxane(PDMS),enhanced polarization loss relaxations were created,which could not only improve the depletion efficiency of EMWA,but also contribute to the optimized impedance matching at elevated temperature.Therefore,the TiN nanotubes/PDMS composite showed excellent EMWA performances at varied temperature(298-573 K),while achieved an effective absorption bandwidth(EAB)value of 3.23 GHz and a minimum reflection loss(RLmin)value of−44.15 dB at 423 K.This study not only clarifies the relationship between dielectric loss capacity(conduction loss and polarization loss)and temperature,but also breaks new ground for EM absorbers in wide temperature spectrum based on interface engineering.

关键词： TiN nanotubes Interface engineering Polarization loss Impedance matching Electromagnetic wave absorption performance

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On the Probability of Generating a Primitive Matrix

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Journal of Systems Science & Complexity 2024年第4期37卷 1755-1771页

作者： CHEN jingwei FENG Yong LIU Yang WU Wenyuan Chongqing Key Laboratory of Automated Reasoning and Cognition Chongqing Institute of Green and Intelligent Technology Chinese Academy of Sciences Chongqing College University of Chinese Academy of Sciences Key Laboratory of Advanced Manufacturing Technology of Ministry of Education Guizhou Universityy Information Science and Engineering Chongqing Jiaotong University

Given a k × n integer primitive matrix A（i.e., a matrix can be extended to an n × n unimodular matrix over the integers） with the maximal absolute value of entries ‖A‖ bounded by an integer λ from above, the authors study the probability that the m × n matrix extended from A by appending other m-k row vectors of dimension n with entries chosen randomly and independently from the uniform distribution over {0, 1, · · ·, λ-1} is still primitive. The authors present a complete and rigorous proof of a lower bound on the probability, which is at least a constant for fixed m in the range[k + 1, n-4]. As an application, the authors prove that there exists a fast Las Vegas algorithm that completes a k × n primitive matrix A to an n × n unimodular matrix within expected ?（nωlog ‖A‖） bit operations, where ? is big-O but without log factors, ω is the exponent on the arithmetic operations of matrix multiplication.

关键词： Integer matrix matrix completion probabilistic algorithm unimodular matrix

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综合医院全科医学护理发展研究进展

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护理研究 2022年第20期36卷 3686-3688页

作者：靳敬伟河北医科大学第二医院河北050000

综述国外全科护士职责范围、培养现状,以及国内全科护理发展现状,提出综合性医院全科护士在不断完善自身素质的同时,应对区域医联体内社区卫生服务中心起到引领、示范作用;全面提升社区护理整体水平,真正将慢性病病人从三级综合医院引... 详细信息

综述国外全科护士职责范围、培养现状,以及国内全科护理发展现状,提出综合性医院全科护士在不断完善自身素质的同时,应对区域医联体内社区卫生服务中心起到引领、示范作用;全面提升社区护理整体水平,真正将慢性病病人从三级综合医院引导至社区医疗机构,助推分级诊疗。

关键词：综合医院全科医学全科护士社区护理职责培养综述

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三级综合医院全科医疗科住院病人疾病分析

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护理研究 2023年第11期37卷 2011-2014页

作者：靳敬伟河北医科大学第二医院河北050000

目的:了解三级综合医院全科医疗科住院病人疾病特点,探讨全科医疗科护士应必备的素质。方法:回顾性分析2021年1月—2022年6月我院全科医疗科住院的2869例次病人的来源渠道、疾病种类、多病共存以及预后和转归情况。结果:病人来源渠道多... 详细信息

目的:了解三级综合医院全科医疗科住院病人疾病特点,探讨全科医疗科护士应必备的素质。方法:回顾性分析2021年1月—2022年6月我院全科医疗科住院的2869例次病人的来源渠道、疾病种类、多病共存以及预后和转归情况。结果:病人来源渠道多,以全科门诊为主(64.1%);入院诊断包括疾病种类63个,以未分化疾病为主;出院诊断以神经系统疾病(28.1%)最多;2454例次(85.5%)治愈出院或好转。结论:全科医疗科住院病人多病共存常见,疾病涉及多系统的慢性病、常见病、多发病和未分化疾病。全科医疗科护士应做到多层面技能与知识的结合,是具备多种能力的复合型人才。

关键词：住院病人全科医疗疾病谱护理

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Flow excitation mechanisms of unbalanced impeller forces after pump power-trip of ultra-high head pump-turbines

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Science China(Technological Sciences) 2024年第5期67卷 1602-1611页

作者： FU XiaoLong LI DeYou LV jingwei YANG Biao WANG HongJie WEI XianZhu School of Energy Science and Engineering Harbin Institute of TechnologyHarbin150001China State Key Laboratory of Hydro-Power Equipment Harbin Institute of Large Electrical MachineryHarbin150040China

To elucidate the dynamic mechanisms of unbalanced impellers in ultra-high head pump-turbines(PTs),this study employed a one-and three-dimensional coupled method to simulate the pump power-trip(PPT)process of an ultra-high head PT.The investigation revealed two novel pulsation frequency components,denoted as fDVand fINFT,associated with impeller forces.The pulsation intensities of these components were markedly higher than those of rotor-stator interaction frequency components in ultra-high head PTs.Notably,the fDVcomponents exhibited pulsations at 1–2 times the rated rotation frequency of the impeller,spanning the entire transition period.Meanwhile,the fINFTcomponents constituted a complex frequency band with various frequency values,primarily occurring near conditions(Q=0,n=0,M=0,and d M/dt=0).These two pulsation frequency components were predominantly linked to the unsteady evolution of dean vortices inside the volute and complex transitions of the flow pattern within the impeller,respectively.It is crucial to note that these unbalanced flow-induced impeller axial forces can elevate the risk of accidents where the rotor is subjected to significant upwind axial forces.These findings offer valuable insights into mitigating the risk of rotor lifting due to axial forces during PT events in ultra-high head PTs.

关键词： ultra-high head pump-turbine pump power-trip unbalanced impeller forces pressure pulsations flow pattern transitions

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Deep Reinforcement Learning or Lyapunov Analysis?A Preliminary Comparative Study on Event-Triggered Optimal Control

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IEEE/CAA Journal of Automatica Sinica 2024年第7期11卷 1702-1704页

作者： jingwei Lu Lefei Li Qinglai Wei Fei-Yue Wang IEEE the Department of Industrial Engineering Tsinghua UniversityBeijing 100084China The State Key Laboratory for Management and Control of Complex Systems Institute of AutomationChinese Academy of SciencesBeijing 100190China

Dear Editor,This letter develops a novel method to implement event-triggered optimal control(ETOC) for discrete-time nonlinear systems using parallel control and deep reinforcement learning(DRL), referred to as Deep-ETOC. The developed Deep-ETOC method introduces the communication cost into the performance index through parallel control, so that the developed method enables control systems to learn ETOC policies directly without triggering conditions.

关键词： Deep letter enable

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