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鸦胆子苦素A抑制Wnt/β-catenin抗结肠癌细胞增殖及转移的作用研究

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南京中医药大学学报 2025年第2期41卷 223-232页

作者：盛钰翔蒋捷苑家福包小江郑正秦富豪唐一石江滨南京中医药大学附属南京中医院江苏南京210000 南京中医药大学常熟附属医院江苏苏州215500 扬州大学医学院江苏扬州225001

目的探究鸦胆子苦素A(BA)对结肠癌的抑制作用及机制。方法采用不同浓度BA(0、1、2、5、10、20、40、80μmol·L^(-1))作用于人结肠癌HT-29和HCT116细胞,利用细胞计数试剂盒(CCK-8)检测细胞活力。通过流式细胞术、细胞划痕实验和Transwel... 详细信息

目的探究鸦胆子苦素A(BA)对结肠癌的抑制作用及机制。方法采用不同浓度BA(0、1、2、5、10、20、40、80μmol·L^(-1))作用于人结肠癌HT-29和HCT116细胞,利用细胞计数试剂盒(CCK-8)检测细胞活力。通过流式细胞术、细胞划痕实验和Transwell实验,评估BA对细胞凋亡、细胞周期、侵袭和迁移能力的影响。采用分子对接模拟BA与GSK-3β蛋白的结合,并通过Western blot检测细胞周期、上皮间质转化(EMT)相关蛋白和Wnt/β-catenin信号通路蛋白表达。建立HT-29细胞皮下移植瘤小鼠模型,成瘤后,将小鼠随机分为3组,即空白组,BA低、高剂量组(0.1、0.2 mg·kg^(-1)),每组6只,给药19 d后,处死小鼠,分离肿瘤组织。观察各组肿瘤体积随时间变化;采用Ki67免疫组化法观察各组肿瘤组织细胞增殖情况;Western blot检测Wnt/β-catenin信号通路蛋白表达。结果与空白组相比,BA可显著抑制HT-29和HCT116细胞增殖,IC 50值为10.80μmol·L^(-1)和17.96μmol·L^(-1)。流式细胞术结果显示,BA显著诱导HT-29细胞凋亡(P<0.01,P<0.001),并阻滞细胞周期于S期,伴随周期相关蛋白CDK2和Cyclin A表达减少(P<0.05,P<0.01,P<0.001)。BA抑制细胞迁移和侵袭能力(P<0.05,P<0.01,P<0.001),降低EMT相关蛋白Snail、Vimentin、N-Cadherin的表达(P<0.01,P<0.001),并上调E-Cadherin蛋白表达。此外,BA抑制β-catenin和p-GSK3β蛋白的表达。Wnt激动剂LiCl可显著拮抗BA的抗结肠癌作用;Wnt抑制剂XAV939可增强BA的抗结肠癌作用。而体内实验中,与空白组相比,BA低、高剂量组肿瘤体积显著缩小(P<0.05,P<0.001)。免疫组化结果显示,与空白组相比,BA低、高剂量组肿瘤组织中Ki67表达显著降低(P<0.001)。Western blot结果进一步证明BA在体内抑制Wnt/β-catenin信号通路。结论BA抑制结肠癌HT-29细胞活力、细胞侵袭和迁移,诱导细胞凋亡并引发细胞周期停滞,并且在体内能显著抑制HT-29细胞皮下移植瘤的生长,其作用与抑制Wnt/β-catenin信号通路正相关。

关键词：鸦胆子苦素A 结肠癌增殖转移 Wnt/β-catenin

A nonlinear equivalent circuit method for analysis of passive intermodulation of mesh reflectors

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Chinese Journal of Aeronautics 2014年第4期27卷 924-929页

作者： jiang jie Li Tuanjie Ma Xiaofei Wang Pei School of Electromechanical Engineering Xidian University Xi’an Institute of Space Radio Technology

Passive intermodulation（PIM） has gradually become a serious electromagnetic interference due to the development of high-power and high-sensitivity RF/microwave communication systems, especially large deployable mesh reflector antennas. This paper proposes a field-circuit coupling method to analyze the PIM level of mesh reflectors. With the existence of many metal–metal（MM） contacts in mesh reflectors, the contact nonlinearity becomes the main reason for PIM generation. To analyze these potential PIM sources, an equivalent circuit model including nonlinear components is constructed to model a single MM contact so that the transient current through the MM contact point induced by incident electromagnetic waves can be calculated. Taking the electric current as a new electromagnetic wave source, the far-field scattering can be obtained by the use of electromagnetic numerical methods or the communication link method. Finally, a comparison between simulation and experimental results is illustrated to verify the validity of the proposed method.

关键词： Contact nonlinearity Equivalent circuit Mesh reflector Metal-metal contact Nonlinear distortion Passive intermodulation Scattering

Adaptive functional link network control of near-space vehicles with dynamical uncertainties

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Journal of Systems Engineering and Electronics 2010年第5期21卷 868-876页

作者： Yanli Du Qingxian Wu Changsheng jiang jie Wen College of Automation Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 E R. China

The control law design for a near-space hypersonic vehicle（NHV） is highly challenging due to its inherent nonlinearity,plant uncertainties and sensitivity to *** paper presents a novel functional link network（FLN） control method for an NHV with dynamical thrust and parameter *** approach devises a new partially-feedback-functional-link-network（PFFLN） adaptive law and combines it with the nonlinear generalized predictive control（NGPC） *** PFFLN is employed for approximating uncertainties in *** weights are online tuned based on Lyapunov stability theorem for the first *** learning process does not need any offline training ***,a robust controller with an adaptive gain is designed to offset the approximation ***,simulation results show a satisfactory performance for the NHV attitude tracking,and also illustrate the controller＇s robustness.

关键词： adaptive control system dynamical uncertainties partially feedback functional link network near-space vehicle.

基于Pasternak地基模型的H(t)-T受荷桩受力变形分析

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上海交通大学学报 2025年第1期59卷 60-69页

作者：江杰陈丽君柴文成艾永林欧孝夺龚健广西大学土木建筑工程学院南宁530004 广西大学特色金属材料与组合结构全寿命安全国家重点实验室南宁530004 广西瑞宇建筑科技有限公司南宁530004

水平动荷载H(t)与扭矩T联合作用下的桩基受力变形较为复杂.为了更加准确地分析H(t)-T联合受荷桩的内力与位移,基于Pasternak地基模型考虑桩侧土体剪切效应;引入H(t)-T耦合因子,揭示多向荷载对桩身响应的影响机理;继而利用虚功原理推导... 详细信息

水平动荷载H(t)与扭矩T联合作用下的桩基受力变形较为复杂.为了更加准确地分析H(t)-T联合受荷桩的内力与位移,基于Pasternak地基模型考虑桩侧土体剪切效应;引入H(t)-T耦合因子,揭示多向荷载对桩身响应的影响机理;继而利用虚功原理推导桩身单元综合刚度矩阵;最后采用改进的有限杆单元法获得H(t)-T联合受荷桩内力与位移数值解;并与已有理论解和有限元模拟结果进行对比验证.参数分析表明:土体剪切效应可约束桩身水平位移,但对扭转变形影响较小;水平动荷载增强了桩身抗扭承载力,水平动荷载幅值从0.2Q_(u)增大到1.0Q_(u)时,桩顶扭转角减小了22.6%;增大动荷载无量纲频率会减小桩顶位移和桩身最大弯矩,外荷载动力效应随桩埋深增加而减弱;杆单元模型减少了单元划分数量和计算时间,可有效提高计算效率.

关键词： Pasternak地基模型联合受荷桩有限单元法水平动荷载扭矩

新橙皮苷治疗骨质疏松症的靶点及对骨髓间充质干细胞成骨分化的作用

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中国组织工程研究 2025年第7期29卷 1437-1447页

作者：章镇宇梁秋健杨军韦相宇蒋捷黄林科谭桢广西医科大学第二附属医院广西壮族自治区南宁市530005 广西医科大学广西再生医学重点实验室广西壮族自治区南宁市530021

背景:前期研究发现新橙皮苷可以延缓去卵巢小鼠的骨丢失,具有治疗骨质疏松症的潜力,但其具体作用机制仍有待探究。目的:基于生物信息学和体外细胞实验探索新橙皮苷治疗骨质疏松症的关键靶点与可能机制。方法:从GEO数据库获得骨质疏松症... 详细信息

背景:前期研究发现新橙皮苷可以延缓去卵巢小鼠的骨丢失,具有治疗骨质疏松症的潜力,但其具体作用机制仍有待探究。目的:基于生物信息学和体外细胞实验探索新橙皮苷治疗骨质疏松症的关键靶点与可能机制。方法:从GEO数据库获得骨质疏松症相关的基因表达数据集,通过R语言筛选和分析差异表达基因,从GeneCards和DisGeNET数据库筛选骨质疏松症的相关靶点,从ChEMBL和PubChem数据库筛选新橙皮苷的相关靶点,三者取交集获得共同靶点,使用String数据库构建交集基因的PPI网络,筛选关键目标靶点,使用DAVID数据库进行GO、KEGG富集分析,运用AutoDock软件对新橙皮苷与目标靶点蛋白进行分子对接验证。检测新橙皮苷对C57小鼠骨髓间充质干细胞成骨分化的影响,以完全培养基为空白对照组、成骨诱导液为对照组,含不同浓度(25,50μmol/L)新橙皮苷的成骨诱导液为实验组,在相应时间检测细胞成骨分化过程中碱性磷酸酶的表达、矿化程度、成骨相关基因及目标靶点基因的表达。结果与结论:(1)筛选出差异表达基因9253个,骨质疏松症相关靶点2161个,新橙皮苷相关靶点326个,三者共同靶点53个,53个基因在骨质疏松症样本中皆上调。PPI网络筛选出具有研究意义的目标靶点基因PRKACA。GO功能与KEGG通路富集分析表明,新橙皮苷通过PRKACA靶点治疗骨质疏松症主要依靠蛋白质磷酸化和蛋白质自身磷酸化等生物过程,作用于内分泌抵抗、癌症中的蛋白聚糖、雌激素信号通路等发挥治疗作用。分子对接结果表明,新橙皮苷与目标靶点PRKACA对应的蛋白有一定的结合能力。(2)碱性磷酸酶染色显示新橙皮苷可以促进间充质干细胞成骨分化早期碱性磷酸酶的表达,茜素红染色显示新橙皮苷可以促进间充质干细胞成骨分化的矿化。RT-qPCR检测结果显示,新橙皮苷可以促进碱性磷酸酶、PRKACA、骨钙素mRNA的表达。(3)结果表明,新橙皮苷可能通过雌激素信号通路上的PRKACA靶点促进成骨分化,从而防治骨质疏松症。

关键词：生物信息学骨质疏松症新橙皮苷骨髓间充质干细胞 PRKACA基因

面向创新能力培养的“微机原理”课程教学改革

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教育教学论坛 2025年第3期 69-72页

作者：方针滕国伟张俊杰蒋皆恢上海大学通信与信息工程学院上海200444

课程建设是教育教学改革的重要内容,为有效实现创新人才培养目标,对信息通信类专业学科基础课“微机原理”开展教学改革。针对“微机原理”课程内容繁杂、概念抽象、实践性强的特点,在教学内容设计与教学实施过程中探索理论与实践教学... 详细信息

课程建设是教育教学改革的重要内容,为有效实现创新人才培养目标,对信息通信类专业学科基础课“微机原理”开展教学改革。针对“微机原理”课程内容繁杂、概念抽象、实践性强的特点,在教学内容设计与教学实施过程中探索理论与实践教学融合的有效模式。教学内容设计使课堂授课、课内实验以及课外挑战性项目的内容相互衔接,教学实施中采用以问题为导向的课堂教学模式,以及以挑战性项目为载体的课外培养模式。通过课内外联动,有效激发学生的学习兴趣和实践热情,促进学生的自主学习与创新能力提高。

关键词：挑战性项目问题导向课内外联动

Effect of Oxide Content of Graphene Oxide Membrane on Remarkable Adsorption for Calcium Ions

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Chinese Physics Letters 2021年第10期38卷 22-24页

作者： jie jiang Long Yan Haiping Fang Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China Department of Physics East China University of Science and TechnologyShanghai 200237China

Graphene oxide membranes(GOMs),as one of the most promising novel materials,have gained great interest in the field of ***,the oxygen content of graphene oxide is directly related to its adsorption properties,such as suspension stability,adsorption capacity,and reusability of ***,a series of reduced GOMs with oxygen content from 28%to 12%were conveniently prepared by the thermally reduced and the corresponding interlayer spacing of these membranes changed from 8.0Ato *** prepared GOMs have remarkable Ca^(2+)adsorption capacity,which increases with the oxygen content or interlayer spacing of ***,the max adsorption capacity of the mass ratio between adsorbed Ca2+and pristine GOMs can reach up to 0.481 g/g,which is about one order of magnitude higher than the adsorption capacity of activated sludge,magnetic Fe_(3)O_(4),functionalized silica,zeolite molecular sieve,and other reported ***,GOMs show excellent stability and the Ca^(2+)can be easily desorbed by water,so that the GOMs can be *** previous theoretical analysis suggests that this remarkable adsorption is attributable to the strong interactions between Ca^(2+)and GO sheets,including the ion-interactions between Ca2+and aromatic graphitic rings as well as the electrostatic interaction between Ca^(2+)and oxygen-containing groups.

关键词： adsorption stability Adsorption

A Study on the Physical Properties of Banana Straw Based on the Discrete Element Method

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Fluid Dynamics & Materials Processing 2023年第5期19卷 1159-1172页

作者： Sen Zhang jie jiang Yuedong Wang School of Mechanical and Electrical Engineering Kunming University of Science and TechnologyKunming650500China Engineering School of Honghe University Mengzi661199China Yunnan University Research Center of Mechatronics Technology and Applied Engineering of Plateau Agricultural Machinery Mengzi661199China Honghe Agricultural Machinery Research Institute Mengzi661199China

To improve the application of discrete element models(DEM)to the design of agricultural crushers,in this study a new highly accurate model is *** model takes into account the fiber structure,porous nature of the material and the leaf sheath coating *** experimental tests are conducted to determine the required“intrinsic”and basic contact parameters of the considered banana straw materials.A large number of bonding parameters are examined in relation to the particle aggregation model in order to characterize different actual banana *** the particle surface energy contact model,the viscosity characteristics of the crushed material are determined together with the related stacking angle(considered as the main response factor).Through single factor experiment analysis,it is found that when the surface energy is 0.9 J·m-2,the relative error between simulations and physical experiments is 5.288%.

关键词： Banana straw discrete element model contact model surface energy DEM

Finite dimensional global and exponential attractors for a class of coupled time-dependent Ginzburg-Landau equations

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Science China Mathematics 2012年第1期55卷 141-157页

作者： jiang jie WU Hao GUO BoLing Institute of Applied Physics and Computational Mathematics Beijing 100088 China School of Mathematical Sciences and Shanghai Key Laboratory for Contemporary Applied Mathematics Fudan University Shanghai 200433 China

We study a coupled nonlinear evolution system arising from the Ginzburg-Landau theory for atomic Fermi gases near the BCS (Bardeen-Cooper-Schrieffer)-BEC (Bose-Einstein condensation) ***,we prove that the initial boundary value problem generates a strongly continuous semigroup on a suitable phase-space which possesses a global *** we establish the existence of an exponential *** a consequence,we show that the global attractor is of finite fractal dimension.

关键词： time-dependent Ginzburg-Landau equations BCS-BEC crossover global attractor exponentialattractor