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BIOCELL 2022年第2期46卷 417-431页

作者： LI PAN WENTING YI DONGMIN LIANG YULONG ZHAO RANRAN WANG PINGYU WANG YOUJIE LI jiaxuan xin YUNFEI YAN SHUYANG XIE Department of Biochemistry and Molecular Biology Binzhou Medical UniversityYantai264003China Department of Medical Laboratory Yantai Affiliated Hospital of Binzhou Medical UniversityYantai264100China

Tumor progression is usually characterized by proliferation,migration,and angiogenesis,which is essential for supplying both nutrients and oxygen to the tumor ***,targeting angiogenesis has been considered a promising therapeutic strategy for cancer prevention and *** the present study,we demonstrated that in addition to suppressing lung cancer cell proliferation and migration in vitro,10-hydroxycamptothecin(10-HCPT)is also capable of inhibiting angiogenesis in vivo with a miR-181a-dependent ***,by upregulating miR-181a,which in turn downregulating FOXP1,10-HCPT can inhibit the PI3K/Akt/ERK signaling pathwaymediated ***,reduced levels of miR-181a have been found in both lung cancer cell lines and xenograft with concurrently elevated levels of FOXP1,VEGF,bFGF,and *** with the findings from the in vitro experiments,miR-181a impairs neovascularization in our xenograft *** summary,our findings have not only established the anti-oncogenic role of miR-181a in lung cancer angiogenesis but also suggest that 10-HCPT could be a potential therapeutic reagent for lung cancer treatment.

关键词： microRNA-181a 10-hydroxycamptothecin Angiogenesis Lung carcinoma

超声速度向量成像技术评价急性缺血性脑卒中患者颈动脉弹性变化

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血管与腔内血管外科杂志 2020年第1期6卷 19-21页

作者：信嘉轩刘颖程谟国李影牡丹江医学院附属第二医院介入科黑龙江牡丹江157000 牡丹江医学院附属第二医院康复科黑龙江牡丹江157000 牡丹江医学院附属第二医院神经内科黑龙江牡丹江157000

目的探讨超声速度向量成像(velocity vector imaging,VVI)技术评价新发缺血性脑卒中(AIS)患者颈动脉弹性的变化。方法选取牡丹江医学院附属第二医院2018年2月至2019年2月AIS患者80例为观察组。选取同期健康体检者80为对照组,比较两组双... 详细信息

目的探讨超声速度向量成像(velocity vector imaging,VVI)技术评价新发缺血性脑卒中(AIS)患者颈动脉弹性的变化。方法选取牡丹江医学院附属第二医院2018年2月至2019年2月AIS患者80例为观察组。选取同期健康体检者80为对照组,比较两组双侧颈动脉的短轴收缩期峰值速度(Vmax)、峰值应变(Smax)、峰值应变率(SRmax)。结果观察组收缩压、舒张压、胆固醇、甘油三酯和血糖均高于对照组,差异有统计学意义(P<0.05);观察组VVI采集双侧颈动脉的短轴收缩期Vmax、Smax和SRmax均低于对照组,差异有统计学意义(P<0.05)。结论VVI技术检测AIS患者较健康者颈动脉弹性降低。

关键词：超声速度向量成像技术动脉内—中膜厚度急性缺血性脑卒中

Targeting TrkB–PSD-95 coupling to mitigate neurological disorders

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Neural Regeneration Research 2025年第3期20卷 715-724页

作者： xin Yang Yu-Wen Alvin Huang John Marshall Department of Molecular Biology Cell Biology and BiochemistryBrown UniversityProvidenceRIUSA Department of Neurology Warren Alpert Medical School of Brown UniversityProvidenceRIUSA Center for Translational Neuroscience Robert J.and Nancy D.Carney Institute for Brain Science and Brown Institute for Translational ScienceBrown UniversityProvidenceRIUSA

Tropomyosin receptor kinase B(TrkB)signaling plays a pivotal role in dendritic growth and dendritic spine formation to promote learning and *** activity-dependent release of brain-derived neurotrophic factor at synapses binds to pre-or postsynaptic TrkB resulting in the strengthening of synapses,reflected by long-term ***,the association of postsynaptic density protein-95 with TrkB enhances phospholipase Cγ-Ca^(2+)/calmodulin-dependent protein kinaseⅡand phosphatidylinositol 3-kinase-mechanistic target of rapamycin signaling required for long-term *** this review,we discuss TrkB-postsynaptic density protein-95 coupling as a promising strategy to magnify brain-derived neurotrophic factor signaling towards the development of novel therapeutics for specific neurological disorders.A reduction of TrkB signaling has been observed in neurodegenerative disorders,such as Alzheimer's disease and Huntington's disease,and enhancement of postsynaptic density protein-95 association with TrkB signaling could mitigate the observed deficiency of neuronal connectivity in schizophrenia and *** with brain-derived neurotrophic factor is problematic,due to poor pharmacokinetics,low brain penetration,and side effects resulting from activation of the p75 neurotrophin receptor or the truncated TrkB.T1 *** TrkB agonists and antibodies that activate TrkB are being intensively investigated,they cannot distinguish the multiple human TrkB splicing isoforms or cell type-specific *** TrkB–postsynaptic density protein-95 coupling provides an alternative approach to specifically boost TrkB signaling at localized synaptic sites versus global stimulation that risks many adverse side effects.

关键词： Angelman syndrome autism brain-derived neurotrophic factor depression neurodegenerative disorder neurodevelopmental disorder postsynaptic density protein-95 synaptic plasticity TrkB

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经桡动脉途径脾动脉栓塞术经验探讨

中国卫生标准管理 2020年第16期11卷 76-79页

作者：赵瑞峰朱旭瑶信嘉轩李双牡丹江医学院附属第二医院介入科黑龙江牡丹江157011

目的探讨经桡动脉入路行脾动脉栓塞术可行性及经验分享。方法2017年3月—2019年3月我院60例脾功能亢进患者中,20例经桡动脉途径,40例采用经股动脉途径。总结分析术后疗效、白细胞和血小板计数、术后反应、并发症发生情况、平均手术时间... 详细信息

目的探讨经桡动脉入路行脾动脉栓塞术可行性及经验分享。方法2017年3月—2019年3月我院60例脾功能亢进患者中,20例经桡动脉途径,40例采用经股动脉途径。总结分析术后疗效、白细胞和血小板计数、术后反应、并发症发生情况、平均手术时间及平均射线剂量。结果60例患者中,术后3天、7天、1个月、3个月的白细胞和血小板水平均明显高于治疗前;无严重感染、脓肿、门静脉血栓等并发症发生;经桡动脉途径患者与经股动脉途径患者术后复查白细胞及血小板均升至正常水平,疗效无显著性差异(P>0.05)。20例经桡动脉途径手术平均时间(36.90±1.21)min,平均X射线剂量(202.40±3.10)mGy,40例经股动脉途径手术平均时间(36.08±2.52)min,平均X线剂量(199.90±6.71)mGy,无显著性差异(P>0.05)。桡动脉组有2例患者术后随访发现桡动脉闭塞,其中1例术中出现痉挛现象。结论经桡动脉入路行脾动脉栓塞术,患者体验佳、并发症少,操作难度及射线剂量与股动脉差异小,是值得推广的一项新技术。

关键词：桡动脉股动脉脾动脉介入脾功能亢进栓塞术

Recent progress in constructing fluorinated solid-electrolyte interphases for stable lithium metal anodes

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 270-291页

作者： Di Zhang Pengfei Lv Wei Qin xin He Yuanhua He Civil Aircraft Fire Science and Safety Engineering Key Laboratory of Sichuan Province College of Civil Aviation Safety EngineeringCivil Aviation Flight University of ChinaGuanghan 618307China School of Chemical Engineering Sichuan UniversityChengdu 610065China

Lithium metal batteries(LMBs)are emerging as a promising energy storage solution owing to their high energy density and specific ***,the non-uniform plating of lithium and the potential rupture of the solid-electrolyte interphase(SEI)during extended cycling use may result in dendrite growth,which can penetrate the separator and pose significant short-circuit *** a stable SEI is essential for the long-term operation of the ***-rich SEI has garnered significant attention for its ability to effectively passivate electrodes,regulate lithium deposition,and inhibit electrolyte *** the structural components and preparation methods of existing fluorinated SEI is crucial for optimizing lithium metal anode *** paper reviews the research on optimizing LiF passivation interfaces to protect lithium metal *** focuses on four types of compositions in fluorinated SEI that work synergistically to enhance SEI *** instance,combining compounds with LiF can further enhance the mechanical strength and ionic conductivity of the *** metals with LiF significantly improves electrochemical performance at the SEI/anode interface,with a necessary focus on reducing electron tunneling ***,incorporating polymers with LiF offers balanced improvements in interfacial toughness and ionic conductivity,though maintaining structural stability over long cycles remains a critical area for future *** alloys combined with LiF increase surface energy and lithium affinity,challenges such as dendrite growth and volume expansion *** summary,this paper emphasizes the crucial role of interfacial structures in LMBs and offers comprehensive guidance for future design and development efforts in battery technology.

关键词： LiF lithium metal anodes solid-electrolyte interphase interface cycling stability

Research status and prospects of the fractal analysis of metal material surfaces and interfaces

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 20-38页

作者： Qinjin Dai Xuefeng Liu xin Ma Shaojie Tian Qinghe Cui Beijing Advanced Innovation Center for Materials Genome Engineering University of Science and Technology BeijingBeijing 100083China Beijing Laboratory of Metallic Materials and Processing for Modern Transportation University of Science and Technology BeijingBeijing 100083China Laboratory for Advanced Materials Processing of Ministry of Education University of Science and Technology BeijingBeijing 100083China School of Mechanical and Electrical Engineering Xinjiang Institute of EngineeringUrumqi 830023China

As a mathematical analysis method,fractal analysis can be used to quantitatively describe irregular shapes with self-similar or self-affine *** analysis has been used to characterize the shapes of metal materials at various scales and *** methods make it difficult to quantitatively describe the relationship between the regular characteristics and properties of metal material surfaces and ***,fractal analysis can be used to quantitatively describe the shape characteristics of metal materials and to establish the quantitative relationships between the shape characteristics and various properties of metal *** the perspective of two-dimensional planes and three-dimensional curved surfaces,this paper reviews the current research status of the fractal analysis of metal precipitate interfaces,metal grain boundary interfaces,metal-deposited film surfaces,metal fracture surfaces,metal machined surfaces,and metal wear *** relationship between the fractal dimensions and properties of metal material surfaces and interfaces is *** from three perspectives of fractal analysis,namely,research scope,image acquisition methods,and calculation methods,this paper identifies the direction of research on fractal analysis of metal material surfaces and interfaces that need to be *** is believed that revealing the deep influence mechanism between the fractal dimensions and properties of metal material surfaces and interfaces will be the key research direction of the fractal analysis of metal materials in the future.

关键词： metal material surfaces and interfaces fractal analysis fractal dimension homogeneity

Single-cell omics: experimental workflow, data analyses and applications

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Science China Life Sciences 2025年第1期68卷 5-102页

作者： Fengying Sun Haoyan Li Dongqing Sun Shaliu Fu Lei Gu xin Shao Qinqin Wang xin Dong Bin Duan Feiyang xing Jun Wu Minmin Xiao Fangqing Zhao Jing-Dong J.Han Qi Liu Xiaohui Fan Chen Li Chenfei Wang Tieliu Shi Department of Clinical Laboratory the Affiliated Wuhu Hospital of East China Normal University (The Second People's Hospital of Wuhu City) Pharmaceutical Informatics Institute College of Pharmaceutical SciencesZhejiang University Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration (Tongji University) Ministry of EducationOrthopaedic DepartmentTongji HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Frontier Science Center for Stem Cells School of Life Sciences and TechnologyTongji University Translational Medical Center for Stem Cell Therapy and Institute for Regenerative Medicine Shanghai East HospitalBioinformatics DepartmentSchool of Life Sciences and TechnologyTongji University Research Institute of Intelligent Computing Zhejiang Lab Shanghai Research Institute for Intelligent Autonomous Systems Center for Single-cell Omics School of Public HealthShanghai Jiao Tong University School of Medicine National Key Laboratory of Chinese Medicine Modernization Innovation Center of Yangtze River DeltaZhejiang University Center for Bioinformatics and Computational Biology Shanghai Key Laboratory of Regulatory Biologythe Institute of Biomedical Sciences and School of Life SciencesEast China Normal University Beijing Institutes of Life Science Chinese Academy of Sciences Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesCenter for Quantitative Biology (CQB)Peking University Zhejiang Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases Women's HospitalZhejiang University School of Medicine Key Laboratory of Advanced Theory and Application in Statistics and Data Science-MOE School of StatisticsEast China Normal University

Cells are the fundamental units of biological systems and exhibit unique development trajectories and molecular features. Our exploration of how the genomes orchestrate the formation and maintenance of each cell, and control the cellular phenotypes of various organismsis, is both captivating and intricate. Since the inception of the first single-cell RNA technology, technologies related to single-cell sequencing have experienced rapid advancements in recent years. These technologies have expanded horizontally to include single-cell genome, epigenome,proteome, and metabolome, while vertically, they have progressed to integrate multiple omics data and incorporate additional information such as spatial sc RNA-seq and CRISPR screening. Single-cell omics represent a groundbreaking advancement in the biomedical field,offering profound insights into the understanding of complex diseases, including cancers. Here, we comprehensively summarize recent advances in single-cell omics technologies, with a specific focus on the methodology section. This overview aims to guide researchers in selecting appropriate methods for single-cell sequencing and related data analysis.

关键词： single-cell sequencing genome epigenome proteomics metabolomics multimodal spatial transcriptomics CRISPR screening

人UbcH10及其siRNA真核表达载体的构建与表达

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放射免疫学杂志 2009年第4期22卷 397-399页

作者：陈世敏徐春晓刘斌辛家璇刘贤锡济南军区总医院实验诊断科 250031 山东大学医学院生物化学与分子生物学研究所 250012

目的:构建以人泛素结合酶UbcH10基因为基础的真核表达质粒pcDNA3.1(UbcH10)及其siRNA表达载体pGPU6/GFP/Neo(siRNA-UbcH10),并在结肠癌细胞LOVO中表达与鉴定。方法:提取组织总RNA,通过RT-PCR扩增出UbcH10基因,克隆到真核表达载体pcDNA3.1的多克隆位点中,构建UbcH10的真核表达载体。通过siRNA设计软件选取UbcH10基因的siRNA片段合成构建表达载体pGPU6/GFP/Neo(siRNA-UbcH10)。然后采用脂质体转染法将其分别转染LOVO细胞,用RT-PCR在RNA水平和Western Blot在蛋白水平检测其在真核细胞中的表达。结果:测序结果显示克隆UbcH10基因片段序列完全正确;重组质粒转染LO-VO细胞后,检测到pcDNA3.1(UbcH10)转染组UbcH10表达增强,而pGPU6/GFP/Neo(siRNA-UbcH10)转染组UbcH10表达减弱。结论:成功构建真核表达质粒pcDNA3.1(UbcH10)及其siRNA表达载体pGPU6/GFP/Neo(siRNA-UbcH10),并在真核细胞中能有效表达,为今后进一步研究UbcH10的功能和作用机制奠定了基础。

关键词： UbcH10 真核表达 siRNA

Typology and working mechanism of a hybrid power router based on power-frequency transformer electromagnetic coupling with converters

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Protection and Control of Modern Power Systems 2023年第3期8卷 80-96页

作者： Jinmu Lai xin Yin Xianggen Yin jiaxuan Hu Fan Xiao School of Electrical and Information Engineering Zhengzhou UniversityZhengzhou 450001Henan ProvincePeople’s Republic of China School of Automation Wuhan University of TechnologyWuhan 430070Hubei ProvincePeople’s Republic of China School of Electrical and Electronic Engineering Huazhong University of Science and Technology(HUST)Wuhan 430074Hubei ProvincePeople’s Republic of China State Grid Hubei Electric Power Research Institute Wuhan 430077Hubei ProvincePeople’s Republic of China

The power router(PR)is a promising piece of equipment for realizing multi-voltage level interconnection and flex-ible power control in the future distribution power *** this paper,a hybrid PR(HPR)topology based on power-frequency transformer electromagnetic coupling with converters is proposed for the medium distribution power *** power-frequency transformer is used to undertake power transmission,voltage conversion,and other main tasks,while the power electronic converters are combined to achieve active *** magnetic and electrical circuit models are established to help discuss the operating principle of the proposed ***,the power flow and control principle of the HPR in different operating conditions are analyzed,with the control system design scheme *** theoretical analysis results are verified by MATLAB/Simulink+Plecs simulation and a controller hardware-in-the-loop study,as well as a down-scale experimental test,indicating that the proposed HPR is flexible in active voltage support and current control.

关键词： Power router Power-frequency transformer Power electronic converter Hybrid Working mechanism

Roles of pH in the NH_(4)^(+)-induced corrosion of AZ31 magnesium alloy in chloride environment

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Journal of Magnesium and Alloys 2022年第11期10卷 3167-3178页

作者： Feng Ge jiaxuan Yin Yue Liu Wenjun Leng xin Wang Zhongyu Cui School of Materials Science and Engineering Ocean University of ChinaQingdao 266100China

Corrosion behavior of AZ31 magnesium alloy in NH_(4)^(+)-bearing chloride solution with controlled pH was investigated by weight loss experiment, hydrogen collection, and electrochemical test combined with surface morphology and topography observation. The results show that NH_(4)^(+) and pH of the solution can affect the corrosion of AZ31 magnesium alloy. The existence of NH4+affects the formation and dissolution of corrosion products, which makes the protective effect of the corrosion product layer weaker. The change of pH value not only affects the corrosion mechanism of AZ31, but also alters the concentration of NH_(4)^(+) ions in the solution, which further influences the corrosion product layer of AZ31, and finally makes the corrosion of AZ31 change significantly.

关键词： NH_(4)^(+) pH AZ31 magnesium alloy Corrosion