检索结果-南通市图书馆

Nrf2蛋白在维吾尔族妇女宫颈鳞癌中的表达及意义

在线全文

学校读者我要写书评

暂无评论

新疆医科大学学报 2016年第4期39卷 411-417页

作者：焦淑娟郑士奇王世雯阿仙姑·哈斯木尼加提·热合木新疆医科大学基础医学院病理解剖学教研室乌鲁木齐830011 新疆医科大学基础医学院乌鲁木齐830011

目的探究Nrf2蛋白在维吾尔族宫颈鳞癌组织中的表达以及对宫颈癌细胞增殖、迁移、侵袭和凋亡等生物学行为的影响。方法运用免疫组织化学方法检测宫颈鳞癌组织、宫颈上皮内瘤变(CIN)和正常宫颈组织中Nrf2与Keap1蛋白的表达。在细胞水平,... 详细信息

目的探究Nrf2蛋白在维吾尔族宫颈鳞癌组织中的表达以及对宫颈癌细胞增殖、迁移、侵袭和凋亡等生物学行为的影响。方法运用免疫组织化学方法检测宫颈鳞癌组织、宫颈上皮内瘤变(CIN)和正常宫颈组织中Nrf2与Keap1蛋白的表达。在细胞水平,运用基因沉默技术下调SiHa细胞中NFE2L2基因的表达,并通过qRTPCR和蛋白印迹法检测NFE2L2mRNA和蛋白水平相应变化;Transwell小室实验及流式细胞术等技术检测转染NFE2L2-siRNA后SiHa细胞迁移、侵袭、凋亡及细胞周期的改变。结果免疫组化结果显示维吾尔族宫颈鳞癌组织中Nrf2蛋白的阳性表达率(66.3%)明显高于CIN组织(56.5%)和正常宫颈组织(27.3%)(P<0.001);转染NFE2L2-siRNA下调宫颈癌SiHa细胞中NFE2L2基因的表达后,NFE2L2 mRNA水平和蛋白水平表达下降,同时SiHa细胞的迁移的细胞数目(103.00±37.58/125.33±34.67)和侵袭数目(82.25±23.56/54.75±9.07)较空白对照组和阴性对照组明显减少(P<0.01),细胞周期阻滞在G0/G1期,SiHa细胞凋亡率显著增加(P<0.01)。结论维吾尔族宫颈鳞癌组织中存在Keap1-Nrf2通路的激活,NFE2L2基因沉默可明显抑制宫颈癌SiHa细胞的增殖、迁移及侵袭能力,并促进细胞凋亡。

关键词：宫颈鳞癌 Nrf2 RNA干扰

基于XML元数据交换任务的国际科技系统间互操作——以JST亚洲科技门户电子期刊和ISTIC精品期刊为例

在线全文

学校读者我要写书评

暂无评论

数字图书馆论坛 2011年第3期 24-29页

作者：姚长青李颖严莹莹焦淑娟乔晓东加藤齐史加藤多惠子中国科学技术信息研究所北京100038 日本科学技术振兴机构日本东京102—8666

在中国，将基于XML元数据互操作技术实际应用到国际化规模性科技信息系统之间的案例并不多见。在遵循国际元数据有关标准的前提下，需要分析合作双方各家元数据的体系结构，再从概念层面对每一元素进行互映射，开发基于XML元数据交换的... 详细信息

在中国，将基于XML元数据互操作技术实际应用到国际化规模性科技信息系统之间的案例并不多见。在遵循国际元数据有关标准的前提下，需要分析合作双方各家元数据的体系结构，再从概念层面对每一元素进行互映射，开发基于XML元数据交换的互操作系统。这些都是必不可少的处理环节。我们以日本科学技术振兴机构运维的亚洲科技门户电子期计检索网站为平台，在中国科学技术信息研究所承担的科技部科技精品期刊项目研究成果的基础上，启动并实施了基于XML元数据交换任务的国际化科技系统问的互操作工作，本文就上述研发内容进行介绍。

关键词：亚洲科技门户 ASTP 精品期刊 XML元数据互操作 JST ISTIC 国际合作

Designing new low alloyed Mg-RE alloys with high strength and ductility via high-speed extrusion

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Minerals,Metallurgy and Materials 2023年第1期30卷 82-91页

作者： Jinshu Xie Zhi Zhang shujuan Liu Jinghuai Zhang Jun Wang Yuying He Liwei Lu Yunlei jiao Ruizhi Wu Key Laboratory of Superlight Material and Surface Technology Ministry of EducationCollege of Materials Science and Chemical EngineeringHarbin Engineering UniversityHarbin 150001China School of Materials Science and Engineering Harbin Institute of TechnologyHarbin 150001China Ningbo Branch Ordnance Science Institute of ChinaNingbo 315103China School of Materials Science and Engineering Hunan University of Science and TechnologyXiangtan 411201China

Two new low-alloyed Mg-2RE-0.8Mn-0.6Ca-0.5Zn(wt%,RE=Sm or Y)alloys are developed,which can be produced on an in-dustrial scale via relatively high-speed *** two alloys are not only comparable to commercial AZ31 alloy in extrudability,but also have superior mechanical properties,especially in terms of yield strength(YS).The excellent extrudability is related to less coarse second-phase particles and high initial melting point of the two as-cast *** high strength-ductility mainly comes from the formation of fine grains,nano-spaced submicron/nano precipitates,and weak ***,it is worth noting that the YS of the two alloys can maintain above 160 MPa at elevated temperature of 250°C,significantly higher than that of AZ31 alloy(YS:45 MPa).The Zn/Ca solute segregation at grain boundaries,the improved heat resistance of matrix due to addition of RE,and the high melting points of strengthening particles(Mn,MgZn_(2),and Mg-Zn-RE/Mg-Zn-RE-Ca)are mainly responsible for the excellent high-temperature strength.

关键词： magnesium alloys high-speed extrusion high strength high ductility solute segregation

Development of high mechanical properties and moderate thermal conductivity cast Mg alloy with multiple RE via heat treatment

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2018年第7期34卷 1076-1084页

作者： Guoqiang Li Jinghuai Zhang Ruizhi WU Yan Feng shujuan Liu Xiaojun Wang Yufeng jiao Qiang Yang Jian Meng Key Laboratory of Superlight Material and Surface Technology Ministry of Education College of Material Science and Chemical Engineering Harbin Engineering University Harbin 150001 China Department of Materials Physics and Chemistry Harbin Institute of Technology Harbin 150001 China College of Materials Science and Engineering Jiamusi University Jiamusi 154007 China State Key Laboratory of Rare Earth Resources Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China College of Science Heihe University Heihe University Heihe 164300 China

A new cast Mg-2Gd-2Nd-2Y-1Ho-1Er-0.5Zn-0.4Zr （wt%） alloy was prepared by direct-chill semicontinuous casting technology. The microstructure, mechanical properties and thermal conductivity of the alloy in as-cast, solid-solution treated and especially peak-aged conditions were investigated. The as-cast alloy mainly consists of α-Mg matrix, （Mg, Zn）3 RE phase and basal plane stacking faults. After proper solid-solution treatment, the microstructure becomes almost Mg-based single phase solid solution except just very few RE-riched particles. The as-cast and solid-solution treated alloys exhibit moderate tensile properties and thermal conductivity. It is noteworthy that the Mg alloy with 8 wt% multiple RE exhibits remarkable age-hardening response （AHV=35.7）, which demonstrates that the multiple RE （RE = Gd, Nd, Y, Ho, Er） alloying instead of single Gd can effectively improve the age-hardening response. The peak-aged alloy has a relatively good combination of high strength/hardness （UTS （ultimate tensile strength）〉 300 MPa; TYS （tensile yield strength）〉210 MPa; 115.3 HV）, proper ductility （ε≈ 6%） and moderate thermal conductivity （52.5 W/（m K））. The relative mechanisms mainly involving aging precipitation of β″ and β＂ phases were discussed. The results provide a basis for development of high performance cast Mg alloys.2017 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science ＆ Technology.

关键词： Mg alloys Age hardening behavio Microstructure Mechanical properties Thermal conductivity

Determination of venlafaxine and its active metabolite O-desmethylvenlafaxine in human plasma by HPLC fluorescence

在线全文

学校读者我要写书评

暂无评论

General Psychiatry 2018年第5期31卷 91-99页

作者： shujuan Shen Chen Zhang Yemeng Mao Shanghai Mental Health Center Shanghai Jiao Tong University

Background Therapeutic drug monitoring guides clinical individualised medication by measuring plasma concentration, which could improve the curative effect, avoid drug overdose and reduce the incidence of adverse reactions. At present, there are few reports on the clinical detection of venlafaxine and its active metabolite O-desmethylvenlafaxine. In this paper, the detection method of venlafaxine and O-desmethylvenlafaxine in blood plasma was established, which provides an effective and convenient means for guiding clinical application of *** To establish a method for determination of venlafaxine and its active metabolite O-desmethylvenlafaxine in human plasma by highperformance liquid chromatography with fluorescence *** Chromatographic separation was achieved on an Agilent Eclipse XDB-C18 Column(4.6 × 150 mm, 5 μm) with water containing sodium dihydrogen phosphate(0.05 mol/L) and acetonitrile(72:28) as the mobile phases. The following parameters were employed: flow rate 0.5 mL/min, column temperature 30°C, fluorescence excitation wavelength 276 nm and emission wavelength 598 *** The method showed good linearity in the concentration range 10–1000 ng/mL. The regression equation for venlafaxine was R=0.0054 C+0.0264, r2=0.99991. The regression equation for O-desmethylvenlafaxine was R=0.0034 C+0.0272, r2=0.99969. The intraday and interday precisions(relative SD) were less than 10%, and the quantitative limit was 10 ng/*** We established a sensitive, specific and simple method for the detection of venlafaxine and O-desmethylvenlafaxine. This method fully meets the needs of clinical trials of venlafaxine and the requirements of relevant guidelines. It provided a reference for the clinical detection of venlafaxine and O-desmethylvenlafaxine plasma concentrations and pharmacokinetic study.

关键词： guides clinical individualised O-desmethylvenlafaxine

Comparative proteomic analysis of cold responsive proteins in two wheat cultivars with different tolerance to spring radiation frost

在线全文

学校读者我要写书评

暂无评论

Frontiers of Agricultural Science and Engineering 2014年第1期1卷 30-38页

作者： shujuan ZHANG Guoqi SONG Yulian LI Jie GAO jiao WANG Guiju CHEN Haosheng LI Genying LI Zhendong ZHAO Crop Research Institute Shandong Academy of Agricultural SciencesKey Laboratory of Wheat Biology&Genetic Improvement on North Yellow&Huai River ValleyMinistry of Agriculture of the People Republic of ChinaNational Engineering Laboratory for Wheat&MaizeJinan 250100China

Spring radiation frost(SRF)is a severe environmental stress which impairs wheat yield and productivity *** better understand the mechanism of wheat(Triticum aestivum)responding to SRF,a comparative proteomic analysis was performed to analyze the changes of the key proteins in two wheat cultivars Jimai22 and Luyuan301 with high and low tolerance to SRF respectively.A total of 43 differentially expressed proteins(DEPs)which mainly involved in carbohydrate metabolism,amino acid metabolism,resistance proteins and antioxidant enzymes,photosynthesis and cellular respiration proteins,cell-wall related proteins,protein translation/processing/degradation and signal transduction were isolated and identified by two-dimensional electrophoresis and MALDI-TOF-TOF *** results revealed that of the 21 DEPs in Jimai22 responding to the SRF,13 DEPs were upregulated and 8 DEPs were downregulated,and that of the 22 DEPs in Luyuan301,9 DEPs were upregulated and 13 DEPs were *** DEPs might be responsible for the stronger cold resistance of Jimai22 compared to *** expression pattern and function analysis of these DEPs were very significant to understanding the mechanism of the SRF responses in wheat.

关键词： common wheat spring radiation frost proteomic analysis 2-DE MALDI-TOF-TOF MS

Ischemic stroke susceptibility gene in a Northern Han Chinese population

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2013年第20期8卷 1881-1891页

作者： Haiping Wang shujuan Shi Wenjing Yan Yan Song Jingjing Zhan Chen Zhang Haiji Wang Department of Neurology Affiliated Hospital of Qingdao University Medical College Health Care Office Affiliated Hospital of Qingdao University Medical College Department of Gerontology Affiliated Hospital of Qingdao University Medical College Department of Internal Medicine Ward 1 Jiao Nan People's Hospital

Interleukin-18 gene promoter polymorphisms are potential risk factors for ischemic cerebrovascular disease, and the –607C allele may increase ischemic stroke risk in the Han Chinese population. In the present study, we recruited 291 patients with ischemic cerebrovascular disease from the Affiliated Hospital of Qingdao University Medical College, China, and 226 healthy controls. Both patients and controls were from the Han population in northern China. Immunoresonance scattering assays detected increased serum amyloid A protein, C-reactive protein, and interleukin-18 levels in ischemic cerebrovascular disease patients compared with healthy controls. Analysis of the –607C/A （rs1946518） polymorphism in the interleukin-18 gene promoter showed ischemic cerebrovascular disease patients exhibited increased frequencies of the CC genotype and C alleles than healthy controls. Genotype and allele frequencies of the interleukin-18 –137G/C （rs187238） polymorphism and the –13T/C （rs11024595） polymorphism in the 5＇-flanking region of serum amyloid A, showed no significant difference between the two groups. Multivariate logistic regression analysis on the interleukin-18 promoter A/C genetic locus, for correction of age, gender, history of smoking, hypertension, diabetes mellitus, hypercholesteremia, and an ischemic stroke family history, showed ischemic cerebrovascular disease risk in individuals without the A allele （C homozygotes） was 2.2-fold greater than in A allele carriers. Overall, our findings suggest that the –13T/C （rs11024595） polymorphism in the 5′-flanking region of serum amyloid A has no correlation with ischemic cerebrovascular disease, but the C allele of the –607C/A （rs1946518） polymorphism in the interleukin-18 promoter is a high-risk factor for ischemic cerebrovascular disease in the Han population of northern China. In addition, the A allele is likely a protective gene for ischemic cerebrovascular disease.

关键词： neural regeneration brain injury interleukin-18 ischemic cerebrovascular disease atherosclerosis gene polymorphism C-reactive protein serum amyloid A protein inflammation neuroregeneration

基于网络编码的VANETs高效数据分发策略

在线全文

学校读者我要写书评

暂无评论

计算机工程 2016年第5期42卷 118-122,129页

作者：汪淑娟尹娇昆明理工大学信息工程与自动化学院昆明650500

在车载自组织网络(VANETs)中,由于网络环境不稳定,路侧单元(RSU)无法将数据快速、高效、可靠地分发给车辆。针对该问题,提出一种基于网络编码技术的RSU多播重传算法。在RSU多播第1阶段结束后,综合车辆的位置信息、速度、行驶方向以及数... 详细信息

在车载自组织网络(VANETs)中,由于网络环境不稳定,路侧单元(RSU)无法将数据快速、高效、可靠地分发给车辆。针对该问题,提出一种基于网络编码技术的RSU多播重传算法。在RSU多播第1阶段结束后,综合车辆的位置信息、速度、行驶方向以及数据有效性等多重特征,求解最优网络编码组合,多播该组合构造的编码包,以提高数据分发效率。实验结果表明,该算法与不编码和随机线性网络编码重传算法相比,平均数据下载率提高4倍,平均下载时延减少90%,数据分发时延分别减少40%和20%,提升了VANETs中数据分发的性能。

关键词：车载自组织网络路侧单元网络编码数据分发多播

LITTIP/Lgr6/HnRNPK complex regulates cementogenesis via Wnt signaling

在线全文

学校读者我要写书评

暂无评论

International Journal of Oral Science 2023年第3期15卷 437-449页

作者： Tiancheng Li Han Wang Yukun Jiang Shuo Chen Danyuan Huang Zuping Wu Xing Yin Chenchen Zhou Yuyu Li shujuan Zou State Key Laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&West China Hospital of Stomatology Sichuan UniversityChengduChina Department of Orthodontics Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai Research Institute of StomatologyShanghaiChina

Orthodontically induced tooth root resorption(OIRR)is a serious complication during orthodontic *** cementum repair is the fundamental approach for the treatment of *** hormone(PTH)might be a potential therapeutic agent for OIRR,but its effects still lack direct evidence,and the underlying mechanisms remain *** study aims to explore the potential involvement of long noncoding RNAs(lncRNAs)in mediating the anabolic effects of intermittent PTH and contributing to cementum repair,as identifying lncRNA-disease associations can provide valuable insights for disease diagnosis and ***,we showed that intermittent PTH regulates cell proliferation and mineralization in immortalized murine cementoblast OCCM-30 via the regulation of the Wnt *** vivo,daily administration of PTH is sufficient to accelerate root regeneration by locally inhibiting Wnt/β-catenin *** RNA microarray analysis,lncRNA LITTIP(LGR6 intergenic transcript under intermittent PTH)is identified as a key regulator of cementogenesis under intermittent *** isolation by RNA purification(ChIRP)and RNA immunoprecipitation(RIP)assays revealed that LITTIP binds to mRNA of leucine-rich repeatcontaining G-protein coupled receptor 6(LGR6)and heterogeneous nuclear ribonucleoprotein K(HnRNPK)*** cotransfection experiments confirmed that LITTIP plays a structural role in the formation of the LITTIP/Lgr6/HnRNPK ***,LITTIP is able to promote the expression of LGR6 via the RNA-binding protein ***,our results indicate that the intermittent PTH administration accelerates root regeneration via inhibiting Wnt *** lncRNA LITTIP is identified to negatively regulate cementogenesis,which activates Wnt/β-catenin signaling via high expression of LGR6 promoted by HnRNPK.

关键词： involvement diagnosis cement