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*** as a model system for Parkinson disease

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neuroscience Bulletin 2006年第2期22卷 124-128页

作者： Pu PU,Wei-Dong LE state key laboratory of Medical Genomics,Ruijin Hospital,shanghai jiao Tong University School of Medicine institute of Health sciences,shanghai institutes for Biological sciences,chinese academy of sciences-shanghai jiao Tong University School of Medicine,shanghai 200031,china state key laboratory of Medical Genomics Ruijin Hospital Shanghai Jiao Tong University School of Medicine institute of Health sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences- Shanghai Jiao Tong University School of Medicine Shanghai 200031China E-mail: weidong@bcm.tmc.edu celegans@***.

Parkinson disease(PD) is characterized by the selective loss of dopaminergic neurons in the substantia nigra. Although investigation in mammalian animal models of PD has enhanced our understanding of PD, the complexity of the mammalian nervous system and our inability to visualize DA neurons in vivo restricts the advances in elucidating the molecular mechanisms of PD. Conservation between C. elegans and mammals in genomic, biosynthetic and metabolic pathways as well as the advantages of observing DA neurons morphology in vivo and the ease of transgenic and genetic manipulation make C. elegans an excellent model organism for PD.

关键词： caenorhabditis elegans( C. elegans) Parkinson disease dopaminergic neuron

Direct lineage conversion:induced neuronal cells and induced neural stem cells

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Protein & Cell 2012年第11期3卷 826-833页

作者： Zixiao Shi jianwei jiao institute of Zoology State Key Laboratory of Reproductive BiologyChinese Academy of SciencesBeijing 100101China

Cellular reprogramming to neural cells is an area of ongoing study in developmental neuroscience,and recent research has generated remarkable *** studies have shown that the ectopic expression of specific neural transcription factors can convert terminally differentiated cells into neural ***,we review the most recent progress in the field of induced neuronal(iN)cells and induced neural stem(iNS)cells and their potential clinical applications.

关键词： iN cells iNS cells conversion reprogramming neuron fibroblast

Effects of cholesterol on neuronal apoptosis via modulation of IK channels

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Effects of cholesterol on neuronal apoptosis via modulation ...

Expert consensus on pediatric orthodontic therapies of malocclusions in children

中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Meng-Hua ZHOU,Song jiao,Yan-Ai MEI~* institute of Brain Science,School of Life sciences and state key laboratory of Medical Neurobiology,Fudan University, shanghai 200433,china

The lipid rafts on the cell membrane which are associated with various diseases,are very important structures for modulating intracellular signal transduction and ion channel *** previous results have revealed that two types of K channels in the rat granule neurons(GC) were up-regulated during neuron apoptosis induced by a medium treatment of low potassium without serum(LK-S).However,no evidence has demonstrated whether this change involves the lipid rafts of neuron *** the present study,we observed that the change in the lipid raft component using the cholesterol or methyl-β-cyclodextrin(MβCD) modulated the cerebellar granule cells’ sensitivity to the LK-S *** the cholesterol(15μM) in the bath solution significantly reduced the viable rate of neurons under the LK-S medium control from(52.7±6.00)%to(29.3±5.20)%.On the other hand,depleting membrane cholesterol by MβCD(500μM) could increase the viable rate in the same treatment condition to(83.1±5.60)%.Neither the enhancement nor the depletion of cholesterol induced neural apoptosis in normal culture *** our previous studies have indicated,IK channels are involved in apoptosis,and Kv2.1channel is the main component of IK channels in *** therefore examined whether cholesterol-induced neural apoptosis occurred via the modulation of IK ***-cell current recording revealed that the application of cholesterol(15μM)) drastically increased the IK current[current amplitude increased from(673.1±32.8) pA to(957.1±42.9) pA].However,cholesterol depletion by MβCD(500μM) decreased the IK current under LK-S treatment. Applying cholesterol or depleting membrane cholesterol does not modify the kinetic properties of the IK current. Furthermore,addition of cholesterol to the culture medium promoted the expression and induced the clustering of the Kv2.1α-subunit on cell membranes,as detected by western blotting and immunostaining *** results suggest that these changes in the compon

关键词： cholesterol neuronal apoptosis Kv2.1 IK cerebellar granule cell

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International Journal of Oral Science 2024年第2期16卷 186-196页

作者： Chenchen Zhou Peipei Duan Hong He Jinlin Song Min Hu Yuehua Liu Yan Liu Jie Guo Fang Jin Yang Cao Lingyong Jiang Qingsong Ye Min Zhu Beizhan Jiang Wenhua Ruan Xiao Yuan Huang Li Rui Zou Yulou Tian Li Gao Rui Shu jianwei Chen Renkai Liu Shujuan Zou Xiaobing Li state key laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Pediatric Dentistry West China Hospital of StomatologySichuan UniversityChengduChina state key laboratory of Oral Diseases&National Center for Stomatology&National Clinical Research Center for Oral Diseases&Department of Orthodontics West China Hospital of StomatologySichuan UniversityChengduChina state key laboratory of Oral and Maxillofacial Reconstruction and Regeneration&key laboratory of Oral Biomedicine Ministry of Education&Hubei key laboratory of Stomatology&Department of Orthodontics&Center for Dentofacial Development and Sleep Medicine School and Hospital of StomatologyWuhan UniversityWuhanChina Chongqing key laboratory of Oral Diseases and Biomedical sciences&Chongqing Municipal key laboratory of Oral Biomedical Engineering of Higher Education Stomatological Hospital of Chongqing Medical UniversityChongqing Medical University&College of StomatologyChongqing Medical UniversityChongqingChina Department of Orthodontics Hospital of StomatologyJilin UniversityChangchunChina Department of Orthodontic&Oral Biomedical Engineering laboratory Shanghai Stomatological HospitalFudan UniversityShanghaiChina Department of Orthodontics Central LaboratoryPeking University School and Hospital for Stomatology&National Center for Stomatology&National Clinical Research Center for Oral Diseases&National Engineering Research Center of Oral Biomaterials and Digital Medical Devices&Beijing Key Laboratory of Digital Stomatology&Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health&NMPA Key Laboratory for Dental Materials&National Engineering Research Center of Oral Biomaterials and Digital Medical DevicesBeijingChina Department of Orthodontics School and Hospital of StomatologyCollege of MedicineShandong University&Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering Laboratory for Dental Materials and Oral Tissue

Malocclusion,identified by the World Health Organization(WHO)as one of three major oral diseases,profoundly impacts the dental-maxillofacial functions,facial esthetics,and long-term development of~260 million children in *** its physical manifestations,malocclusion also significantly influences the psycho-social well-being of these *** intervention in malocclusion can foster an environment conducive to dental-maxillofacial development and substantially decrease the incidence of malocclusion or reduce the severity and complexity of malocclusion in the permanent dentition,by mitigating the negative impact of abnormal environmental influences on the *** orthodontic treatment encompasses accurate identification and treatment of dental and maxillofacial morphological and functional abnormalities during various stages of dental-maxillofacial development,ranging from fetal stages to the early permanent dentition *** an economic and societal standpoint,the urgency for effective early orthodontic treatments for malocclusions in childhood cannot be overstated,underlining its profound practical and social *** consensus paper discusses the characteristics and the detrimental effects of malocclusion in children,emphasizing critical need for early *** elaborates on corresponding core principles and fundamental approaches in early orthodontics,proposing comprehensive guidance for preventive and interceptive orthodontic treatment,serving as a reference for clinicians engaged in early orthodontic treatment.

关键词： permanent stated children

Insight into the formation mechanism and interaction of matrix/TiB whisker textures and their synergistic effect on property anisotropy in titanium matrix composites

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Journal of Materials Science & Technology 2022年第15期110卷 1-13页

作者： Jianwen Le Yuanfei Han Peikun Qiu Shaopeng Li Guangfa Huang jianwei Mao Weijie Lu state key laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China shanghai key laboratory of Advanced High Temperature Materials and Precision Forming Shanghai 200240China shanghai jiao Tong University Baotou institute of Material Research Baotou 014020China

Considerable studies on processed pure titanium and titanium alloys have proved the possibility of prop-erty anisotropy induced by crystallographic textures,but limited information is available for the intrinsic coupling of matrix and reinforcement textures and their synergistic effect on property anisotropy in tita-nium matrix composite(TMCs).In the present work,an advanced EBSD/EDS coupling method was used to investigate the formation mechanism of primaryαand secondaryαtextures in the matrix *** is revealed for the first time that the reinforcement TiB_(w)displays a{100}texture after hot rolling and has little effect on the matrix texture component but weakens texture *** anisotropies in the tensile strength and ductility can be all noted at room and high-temperatures,which is the syn-ergistic effect of the matrix texture and the aligned TiB_(w).The mean Schmid factor of each slip system was calculated to evaluate the influence of matrix texture on the minimum active stress of slip deforma-tion in the different tensile *** analysis shows that the strong T-type matrix texture results in higher strength but lower ductility when loaded in the transverse ***,a generalized shear-lag model was modified to quantitatively evaluate the strengthening contribution of aligned TiB_(w),which decreases with increasing off-axis angle and test temperature.A new parameter,defined as the critical aspect ratio of the off-axis whisker,was proposed to rationalize why the TiB_(w) failure mechanism converts from TiB_(w) fracture to TiB_(w)/matrix interfacial debonding with increasing off-axis angle and test temperature.

关键词： Titanium matrix composites(TMCs) TiB whiskers Anisotropy Mechanical properties Texture

Cancer cell employs a microenvironmental neural signal transactivating nucleus-mitochondria coordination to acquire stemness

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Signal Transduction and Targeted Therapy 2023年第8期8卷 3818-3836页

作者： Bin He Rui Gao Shasha Lv Ailin Chen Junxiu Huang Luoxuan Wang Yunxiu Feng Jiesi Feng Bing Liu Jie Lei Bing Deng Bin He Bai Cui Fei Peng Min Yan Zifeng Wang Eric W-F Lam Bilian Jin Zhiming Shao Yulong Li jianwei jiao Xi Wang Quentin Liu state key laboratory of Oncology in South china Sun Yat-sen University Cancer CenterGuangzhou 510060PR China Department of Medical Oncology The Seventh Affiliated HospitalSun Yat-sen UniversityShenzhen 510275PR China institute of Cancer Stem Cell Cancer CenterDalian Medical UniversityDalian 116023PR China Zhongshan School of Medicine Sun Yat-sen UniversityGuangzhou 510080PR China state key laboratory of Membrane Biology Peking University School of Life SciencesBeijing 100871PR China Department of Surgery and Cancer Imperial College LondonLondon W120NNUK Department of Breast Surgery Precision Cancer Medicine CenterFudan University Shanghai Cancer CenterShanghai 200032PR China state key laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101PR China

Cancer cell receives extracellular signal inputs to obtain a stem-like status,yet how tumor microenvironmental(TME)neural signals steer cancer stemness to establish the hierarchical tumor architectures remains ***,a pan-cancer transcriptomic screening for 10852 samples of 33 TCGA cancer types reveals that cAMP-responsive element(CRE)transcription factors are convergent activators for cancer *** of transcriptomic profiles,specification of neural markers and illustration of norepinephrine dynamics uncover a bond between TME neural signals and cancer-cell CRE ***,neural signal norepinephrine potentiates the stemness of proximal cancer cells by activating cAMP-CRE axis,where ATF1 serves as a conserved *** activation by norepinephrine,ATF1 potentiates cancer stemness by coordinated trans-activation of both nuclear pluripotency factors MYC/NANOG and mitochondrial biogenesis regulators NRF1/TFAM,thereby orchestrating nuclear reprograming and mitochondrial ***,single-cell transcriptomes confirm the coordinated activation of nuclear pluripotency with mitochondrial biogenesis in cancer stem-like *** findings elucidate that cancer cell acquires stemness via a norepinephrine-ATF1 driven nucleus-mitochondria collaborated program,suggesting a spatialized stemness acquisition by hijacking microenvironmental neural signals.

关键词： Cancer neural architectures

Integrative analysis of in vivo recording with single-cell RNA-seq data reveals molecular properties of light-sensitive neurons in mouse V1

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Protein & Cell 2020年第6期11卷 417-432页

作者： jianwei Liu Mengdi Wang Le Sun Na Clara Pan Changjiang Zhang Junjing Zhang Zhentao Zuo Sheng He Qian Wu Xiaoqun Wang state key laboratory of Brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of Brain-Intelligence Technology(Shanghai)Institute of BiophysicsChinese Academy of SciencesBeijing 100101China state key laboratory of Cognitive neuroscience and Learning Beijing Normal UniversityBeijing 100875China University of chinese academy of sciences Beijing 100049China institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China IDG/McGovem institute for Brain Research Beijing Normal UniversityBeijing 100875China Advanced Innovation Center for Human Brain Protection Beijing Institute for Brain DisordersCapital Medical UniversityBeijing 100069China

Vision formation is classically based on projections from retinal ganglion cells(RGC)to the lateral geniculate nucleus(LGN)and the primary visual cortex(V1).Neurons in the mouse V1 are tuned to light *** the cellular information of the retina and the LGN has been widely studied,the transcriptome profiles of single light-stimulated neuron in V1 remain *** our study,in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2/3 of V1 as lightsensitive(LS)or non-light-sensitive(NS)by single-cell light-evoked calcium evaluation and action potential *** contents of each cell after functional tests were aspirated in vivo through a patch-clamp pipette for mRNA ***,the three-dimensional(3-D)morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell *** sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation,such as Rtn4r and Rgs7,and genes involved in membrane transport,such as Na+/K+ATPase and NMDA-type glutamatergic receptors,preferentially responded to light ***,an antagonist that blocks Rtn4r signals could inactivate the neuronal responses to light stimulation in live *** conclusion,our findings of the vivo-seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.

关键词： light sensitivity vivo-seq patch-seq calcium imaging in vivo whole cell recording in vivo

Scorpion toxin BmKI directly activates Nav1.8 in primary sensory neurons to induce neuronal hyperexcitability in rats

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Protein & Cell 2015年第6期6卷 443-452页

作者： Pin Ye Yunlu jiao Zhenwei Li Liming Hua Jin Fu Feng Jiang Tong Liu Yonghua Ji laboratory of Neuropharmacology and Neurotoxicology Shanghai University Shanghai 200436 China Jiangsu key laboratory of Translational Research and Therapy for Neuro-Psycho-Diseases and the Second Affiliated Hospital of Soochow University Institute of Neuroscience Soochow University Suzhou 215021 China

Voltage-gated sodium channels （VGSCs） in primary sensory neurons play a key role in transmitting pain signals to the central nervous system. BmK I, a site-3 sodium channel-specific toxin from scorpion Buthus martensi Karsch, induces pain behaviors in rats. How- ever, the subtypes of VGSCs targeted by BmK I were not entirely clear. We therefore investigated the effects of BmK I on the current amplitude, gating and kinetic properties of Nav1.8, which is associated with neuronal hyperexcitability in DRG neurons. It was found that BmK I dose-dependently increased Nav1.8 current in small- sized （〈25 μm） acutely dissociated DRG neurons, which correlated with its inhibition on both fast and slow in- activation. Moreover, voltage-dependent activation and steady-state inactivation curves of Nay1.8 were shifted in a hyperpolarized direction. Thus, BmK I reduced the threshold of neuronal excitability and increased action potential firing in DRG neurons. In conclusion, our data clearly demonstrated that BmK I modulated Nav1.8 re- markably, suggesting BmK I as a valuable probe for studying Nay1.8. And Navl.8 is an important target re- lated to BmK I-evoked pain.

关键词： voltage-gated sodium channel Nay1.8,primary sensory neurons BmK I

General synthesis of hollow mesoporous conducting polymers by dual-colloid interface co-assembly for high-energy-density micro-supercapacitors

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Journal of Energy Chemistry 2021年第11期30卷 145-152,I0004页

作者： Jing Cui Fei-Fei Xing Hao Luo Jie-Qiong Qin Yan Li Yonghui Zhong Facai Wei jianwei Fu Chengbin Jing Jiangong Cheng Zhong-Shuai Wu Shaohua Liu state key laboratory of Precision Spectroscopy and Department of Materials School of Physics and Electronic ScienceEast China Normal University500 Dongchuan RoadShanghai 200241China state key laboratory of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of Sciences457 Zhongshan RoadDalian 116023LiaoningChina Dalian National laboratory for Clean Energy Chinese Academy of Sciences457 Zhongshan RoadDalian 116023LiaoningChina University of chinese academy of sciences 19 A Yuquan RoadShijingshan DistrictBeijing 100049China School of Chemistry and Molecular Engineering East China Normal University500 Dongchuan RoadShanghai 200241China School of Materials Science and Engineering Zhengzhou University100 Science AvenueZhengzhou 450002HenanChina state key Lab of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of Sciences865 Changning RoadShanghai 200050China shanghai academy of Quality Management 258 Wuyi RoadShanghai 200050China

Rational design and precise regulation over the morphology, structure, and pore size of functional conducting mesoporous polymers with enriched active sites and shorten electron–ion transport pathway are extremely important for developing high-performance micro-supercapacitors (MSCs), but still remain a great challenge. Herein, a general dual-colloid interface co-assembly strategy is proposed to fabricate hollow mesoporous polypyrrole nano-bowls (mPPy-nbs) for high-energy-density solid-state planar MSCs. By simply adjusting the size of block copolymer micelles, the diameter of polystyrene nanospheres and the amount of pyrrole monomer, mesopore size of the shell, void and shell thickness of mPPy-nbs can be simultaneously controlled. Importantly, this strategy can be further utilized to synthesize other hollow mesoporous polymers, including poly(tris(4-aminophenyl)amine), poly(1,3,5-triaminobenzene) and their copolymers, demonstrative of excellent universality. The structurally optimized mPPy-nb exhibits high specific surface area of 122 m^(2) g^(−1)and large capacitance of 225 F g^(−1) at 1 mV s^(−1). Furthermore, the MSCs assembled by mPPy-nbs deliver impressive volumetric capacitance of 90 F cm^(−3) and energy density of 2.0 mWh cm^(−3), superior to the most reported polymers-based MSCs. Also, the fabricated MSCs present excellent flexibility with almost no capacitance decay under varying bending states, and robust serial/parallel self-integration for boosting voltage and capacitance output. Therefore, this work will inspire the new design of mesoporous conducting polymer materials toward high-performance microscale supercapacitive devices.

关键词： Interface co-assembly Dual-colloids Mesopore Hollow nano-bowl Micro-supercapacitors