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[Determination of eight phthalate metabolites in urine of pregnant women and evaluation of neonatal birth outcomes based on solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 60-67页

作者： Zi-Hao Wang Meng-Fei Xu Bei-Ni Li ping Wu Wei Wu Hubei University of Chinese Medicine Wuhan 430065 China.

Phthalates (PAEs) are endocrine-disrupting chemicals that are widely present in everyday life and enter the human body through various pathways. The release of PAEs into the environment through pathways that include leaching, evaporation, abrasion, and the use of personal care products exposes humans to PAEs via ingestion, inhalation, and dermal absorption. Pregnant women, as a particularly vulnerable population, risk adverse newborn growth and development when exposed to PAEs. While the concentrations of PAEs in urine reflect recent exposure levels in humans, urinary levels of phthalate metabolites (mPAEs) are commonly used as biomarkers of internal exposure owing to the relatively short biological half-lives of PAEs (<24 h). In this study, we developed a solid-phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for simultaneously detecting eight mPAEs in the urine of pregnant women. Urine samples were enzymatically hydrolyzed with -glucosidase and then purified using the Bond Elut Plexa SPE column, with subsequent elution, concentration, and redissolved performed prior to HPLC-MS/MS. Separation was achieved using an Agilent Eclipse Plus C18 column (100 mm×3 mm, 3.5 μm), with gradient elution performed using 0.1% acetic acid aqueous solution and 0.1% acetic acid acetonitrile as mobile phases. Multiple reaction monitoring (MRM) mode was used for detection, with quantification performed using the internal-standard method. Good linearities were obtained in the range of 0.1-200 ng/mL for the eight mPAEs, with limits of detection (LODs) and quantification (LOQs) of 0.015-0.048 and 0.050-0.160 ng/mL, respectively. The eight mPAEs exhibited recoveries of 80.2%-99.7% at three spiked levels (1, 10, and 50 ng/mL). This method was subsequently used to analyze the eight mPAEs levels in urine samples of 497 pregnant women from the Ezhou Maternity and Child Health Care Hospital. The participants exhibited widespread exposure t

关键词： birth outcomes high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) phthalate metabolites (mPAEs) urine samples of pregnant women

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Inhibitory effects of reserpine and carbonyl cyanide m-chloro-phenylhydrazone on fluoroquinolone resistance of Acinetobacter baumannii

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Chinese Medical Journal 2005年第4期118卷 340-343页

作者： SHIWei-feng jiangjian-ping XUNing HUANGZhi-mi WANGYu-yue DepartmentofClinicalLaboratory ThirdAffiliatedHospitalofSuzhouUniversityChangzhou213003China DepartmentofClinicalChemistry UniversityHospitalofLundLundS-22185Sweden DepartmentofClinicalLaboratory 98thPLAHospitalHuzhou313000China

Mechanisms of bacterial resistance to fluoro-quinolones may be grouped intothree principal categories: gene mutations of DNA topoisomerase Ⅱ (GyrA or GyrB), DNA topoisomeraseⅣ ( ParC or ParE), decrease of outer membrane permeation and upregulation of multi-drug effluxpump (active efflux system). Efflux pumps are transport proteins removing toxic substrates(including virtually all classes of clinically relevant antibiotics) from cells to the externalenvironment. These proteins exist in both Gram positive bacteria and Gram negative bacteria as wellas in fungi and mammalian (tumour) cells. It has been reported that alkaloid reserpine and carbonylcyanide m-chlorophenylhydrazone (CCCP) can inhibit NorA multi-drug efflux. In order to explore theuniversality of drug efflux in microorganisms, 85 strains of Acinetobacter baumannii (A. baumannii)were tested using reserpine and CCCP. The quinolone-resistant-determining region (QRDR) of gyrA andparC genes in 35 isolates of A. baumannii were amplified by polymerase chain reaction (PCR) andsequenced by DNA sequencer. The correlation between resistant mutation regularity and bacterial drugefflux were analysed.

关键词： acinetobacter baumannii efflux effect reserpine carbonyl cyanidem-chlorophenylhydrazone gyrA parC

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[Recent progress in mass spectrometry imaging using nanospray desorption electrospray ionization]

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Se pu = Chinese journal of chromatography 2025年第1期43卷 43-49页

作者： Jing-Bo Wang Xiao-Lan Li Rui-Xia Fan ping Lü Rui-Chuan Yin West China School of Pharmacy Sichuan University Chengdu 610041 China.

Ambient mass spectrometry imaging (MSI) enables hundreds of analytes in tissue sections to be directly mapped at atmospheric pressure with minimal sample preparation. This field is currently experiencing rapid growth, with numerous reported ambient ionization techniques resulting in a "hundred flowers bloom" situation. Nanospray desorption electrospray ionization (nano-DESI), developed by the Laskin group in 2010, is a widely used liquid-extraction-based ambient ionization technique that was first used for mass spectrometry imaging of tissue in 2012. The nano-DESI probe comprises a primary capillary and a nanospray capillary, with the latter efficiently transferring analyte-containing droplets via a tiny liquid bridge formed between the probe and sample surface, thereby enabling nanoelectrospray ionization (nano-ESI) in front of the inlet of a mass spectrometer. The advantages of nano-DESI MSI include minimal sample preparation, high spatial resolution, and high sensitivity. These features are well-suited for imaging various sample types, including frozen tissue sections, microbial communities, and environmental samples. A PubMed-database search using the "nano-DESI" keyword revealed 72 related articles in the 2010-2024 period, with 34 of them published between 2021 and 2024, which indicates that nano-DESI has rapidly developed as an ambient ionization technique over recent years. Herein, we briefly introduce key nano-DESI-MSI research progress reported in the past three years with the aim of better understanding and facilitating the use of this technology. We first discuss advances in ion-source development. Since no commercial nano-DESI source exists, designing and constructing ion sources remain technical challenges that limit its development. Nano-DESI has been successfully coupled with various types of mass spectrometer, including LTQ Orbitrap, quadrupole-Orbitrap (Q Exactive), 6560 IM QTOF, timsTOF Pro2, triple quadrupole, and FTICR. These couplings have signi

关键词： ambient mass spectrometry imaging nanospray desorption electrospray ionization spatial proteomics structural characterization of lipid

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Methylammonium cation deficient surface for enhanced binding stability at TiO2/CH3NH3PbI3 interface

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Nano Research 2017年第2期10卷 483-490页

作者： Xin Xu Kai Li Zhenzhong Yang jiangjian Shi Dongmei Li Lin Gu Zhijian Wu Qingbo Meng Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

Heterojunction interfaces in perovskite solar cells play an important role in enhancing their photoelectric properties and stability. Till date, the precise lattice arrangement at TiO2/CH3NH3PbI3 heterojunction interfaces has not been investigated clearly. Here, we examined a TiO2/CH3NH3PbI3 interface and found that a heavy atomic layer exists in such interfaces, which is attributed to the vacancies of methylammonium （MA） cation groups. Further, first-principles calculation results suggested that an MA cation-deficient surface structure is beneficial for a strong heterogeneous binding between TiO2 and CH3NH3PbI3 to enhance the interface stability. Our research is helpful for further understanding the detailed interface atom arrangements and provides references for interfacial modification in perovskite solar cells.

关键词： perovskite interfacial atomic structure scanning transmission electron microscopy (STEM) first-principles calculations

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Distinct MLT asymmetry of auroral kilometric radiation observed by the FAST satellite

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Earth and Planetary Physics 2025年第1期9卷 188-193页

作者： JiaWen Tang Sai Zhang FuLiang Xiao HongMing Yang Si Liu YiHua He Chang Yang YuYue Jin ZhouKun Deng ping Li Archie James Johnston School of Physics and Electronic Sciences Changsha University of Science and TechnologyChangsha 410114China Hunan Province Higher Education Key Laboratory of Modeling and Monitoring on the Near-Earth Electromagnetic Environments Changsha University of Science and TechnologyChangsha 410114China School of Electrical and Information Engineering Changsha University of Science and TechnologyChangsha 410114China

Auroral Kilometric Radiation (AKR) is a common radio emission,which can contribute to the magnetosphere-ionosphereatmosphere co u *** emissions have been observed in all magnetic planet magnetospheres of the solar *** this study,using observations from the FAST satellite from 30 August 1996 to 9 September 2001,the distribution of AKR in altitude=500-4500 km and invariant latitude (|ILAT|)=60°-80°has been analyzed.63045 AKR samples have been identified with~48%(52%) samples on the dayside (nightside).Of considerable interest,there is a distinct MLT asymmetry with the high occurrence rate in MLT=05-08 and 18-22(02-05 and 12-17) in the northern (southern) *** distinct MLT asymmetry is associated with the direction of Bxof the interplaneta ry magnetic *** addition,the occurrence rate on the nightside clearly increases as the AE^(*) index *** study further enriches the information and understanding of AKR in the magnetosphere as well as other similar radio emissions.

关键词： asymmetry auroral kilometric radiation interplanetary magnetic field

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技术调节理论视野下的“师-技”关系及其实践指向

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黑龙江高教研究 2025年第1期43卷 118-123页

作者：周建平绍兴文理学院浙江绍兴312000

从技术调节理论看,“师-技”关系本质上是一种“共塑”关系。在教师使用技术情境中,教师与技术相互形塑,不仅技术调节着教师的经验和行动,而且教师也在“处置”、重构乃至重塑技术。“共塑”论不仅拒斥“替代论”、消除“恐惧论”,而且... 详细信息

从技术调节理论看,“师-技”关系本质上是一种“共塑”关系。在教师使用技术情境中,教师与技术相互形塑,不仅技术调节着教师的经验和行动,而且教师也在“处置”、重构乃至重塑技术。“共塑”论不仅拒斥“替代论”、消除“恐惧论”,而且超越“代具论”和“互补论”,其实践意蕴在于让我们重新认识技术使用中教师的地位,明晰教师的技术使用责任,突破对技术的功能性认识,倡导技术“使用的技艺”,在技术设计中融入教师的教育价值诉求,真正让教师使用好技术。

关键词：技术调节 “师一技”关系 “共塑”

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang Jiao Ming Li Xi Chen Mei-Ze Xu Tian Ding Xiao-ping Gong Zhuo-Mei Li Xue-Yuan Liu Yong-Min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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升降散改善MNNG诱导的胃黏膜损伤及其机制

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中国药理学通报 2025年第1期41卷 178-185页

作者：刘轶璜张思佳刘菲陈苹苹河北中医药大学药学院河北石家庄050091 河北省离子通道功能与创新中药国际联合研究中心河北石家庄050091 河北中医药大学中西医结合学院河北石家庄050091

目的确定升降散对胃黏膜损伤的改善作用并探讨其机制。方法通过文献计量学研究了解升降散的研究现状。采用液质联用技术获取升降散主要药效成分,通过网络药理学分析预测机制。对胃黏膜损伤大鼠灌胃给予阳性药及不同剂量升降散,通过HE染... 详细信息

目的确定升降散对胃黏膜损伤的改善作用并探讨其机制。方法通过文献计量学研究了解升降散的研究现状。采用液质联用技术获取升降散主要药效成分,通过网络药理学分析预测机制。对胃黏膜损伤大鼠灌胃给予阳性药及不同剂量升降散,通过HE染色,观察胃组织形态。ELISA实验检测血清中TNF-α、IL-1β的水平。IHC实验评价胃黏膜MUC5AC、MUC6的表达。结果文献计量学分析表明,升降散在内外伤杂病治疗方面应用广泛。根据升降散主要药效成分中浓度前20位的化合物得到的作用靶点525个,与胃炎相关靶点相交集的129个;GO分析得条目数2127条,KEGG分析得通路140条;说明升降散具有多靶点多通路改善胃黏膜损伤的可能性。HE染色结果显示,升降散确能改善大鼠胃黏膜炎症。ELISA实验结果显示,升降散可有效下调大鼠血清中的TNF-α和IL-1β。IHC实验结果表明,升降散有效下调MUC5AC的表达、上调MUC6的表达。结论升降散能以缓解炎症、调节表面黏液屏障的方式改善胃黏膜损伤。

关键词：升降散慢性胃炎文献计量学网络药理学胃组织结构炎症因子免疫组织化学

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二维光晶格中旋转玻色-爱因斯坦凝聚基态中的涡旋分布

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原子与分子物理学报 2025年第1期42卷 118-126页

作者：张志强王平平吕亚南郑州商学院大学物理教研室郑州451200

利用多重网格预条件共轭梯度法,研究了囚禁在二维光晶格与谐振势组成的复合势阱中的旋转玻色-爱因斯坦凝聚体中的涡旋分布,探讨了光晶格深度、晶格常数和凝聚体旋转频率对旋转凝聚体中涡旋分布的影响.结果表明,由于光晶格势阱的引入,凝... 详细信息

利用多重网格预条件共轭梯度法,研究了囚禁在二维光晶格与谐振势组成的复合势阱中的旋转玻色-爱因斯坦凝聚体中的涡旋分布,探讨了光晶格深度、晶格常数和凝聚体旋转频率对旋转凝聚体中涡旋分布的影响.结果表明,由于光晶格势阱的引入,凝聚体中产生涡旋,且涡旋位于外部势阱极小值处.随着光晶格势阱深度的增加,凝聚体集聚在光晶格势阱的底部,形成格点状分布,其中的涡旋形成涡旋对,并融合形成更大的涡旋.在晶格常数较小时,凝聚体中涡旋分布形成类似Abrikosov涡旋晶格,随着晶格常数的增加,凝聚体中涡旋的分布形式出现多样化,出现了内中外三层结构.随着旋转频率的增加,凝聚体中涡旋数目也随之增加,涡旋分布也更加复杂和富于变化,凝聚体密度分布范围也随着旋转频率的增加而扩展.当凝聚体旋转频率接近外部谐振势阱的谐振频率,即凝聚体处于最低朗道能级近似时,其中的涡旋数量大幅度增加,且出现涡旋成对组合与融合现象,并形成了涡旋斑图花纹.

关键词：波色-爱因斯坦凝聚涡旋二维光晶格复合势阱

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实时直接分析质谱及与样品前处理技术联用的研究进展

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分析测试学报 2025年第1期44卷 43-50页

作者：林雪维姚钟平香港理工大学化学生物学及药物研发国家重点实验室未来食品研究院中医药创新研究中心应用生物与化学科技学系中国香港香港理工大学深圳研究院深圳市中药药学及分子药理学研究重点实验室深圳市食品生物污染与控制重点实验室广东深圳518057

实时直接分析质谱无需或者仅需简单的样品制备,即能对样品进行原位电离及直接快速的进样分析,已被广泛运用于食品、司法科学、药物分析、临床科学、环境等领域。尽管已有不少文献报道DART-MS的定量结果与液相/气相色谱-质谱相当,但其痕... 详细信息

实时直接分析质谱无需或者仅需简单的样品制备,即能对样品进行原位电离及直接快速的进样分析,已被广泛运用于食品、司法科学、药物分析、临床科学、环境等领域。尽管已有不少文献报道DART-MS的定量结果与液相/气相色谱-质谱相当,但其痕量分析物的检测性能仍是关键问题。为获得更准确的定量结果,研究人员开发出了一系列样品前处理技术与DART-MS联用。该文从DART-MS的原理出发,对其不同研究领域中无需样品前处理的应用进行了综述,并进一步介绍了样品预处理技术(如固相萃取、固相微萃取、顶空固相网增强吸附、分散固相萃取、分散磁性固相萃取、机械化学萃取、微波辅助萃取和热解吸)与DART-MS联用的研究成果。该综述旨在对DART-MS的样品直接分析的应用及样品前处理技术的开发提供帮助。

关键词：质谱实时直接分析直接样品分析样品预处理

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