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Journal of Integrative Agriculture 2024年第7期23卷 2164-2177页

作者： Bingli jiang Wei Gao Yating jiang Shengnan Yan Jiajia Cao Litian Zhang yue Zhang Jie Lu Chuanxi Ma Cheng Chang Haiping Zhang Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow&huai River Valley Ministry of Agriculture and Rural Affairs/College of AgronomyAnhui Agricultural UniversityHefei 230036China

The P-type plasma membrane(PM)H^(+)-ATPases(HAs)are crucial for plant development,growth,and *** HAs have been thoroughly characterized in many different ***,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in ***,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five *** examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD *** further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in‘Jimai 22’and‘Jing 411’backgrounds,and in transgenic Arabidopsis ***7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and *** results presented here will enable future research regarding the TaHAs in wheat.

关键词： wheat P-type plasma membrane H^(+)-ATPase seed dormancy abscisic acid gibberellin

Camellia sinensis CsMYB4a participates in regulation of stamen growth by interaction with auxin signaling transduction repressor CsAUX/IAA4

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The Crop Journal 2024年第1期12卷 188-201页

作者： Guoliang Ma Mingzhuo Li Yingling Wu Changjuan jiang Yifan Chen Dawei Xing yue Zhao Yajun Liu Xiaolan jiang Tao Xia Liping Gao School of Life Science Anhui Agricultural UniversityHefei 230036AnhuiChina Plant for Human Health Institute Department of Plant and Microbial BiologyNorth Carolina State UniversityKannapolisNC 28081USA State Key Laboratory of Tea Plant Biology and Utilization/Key Laboratory of Tea Biology and Tea Processing of Ministry of Agriculture/Anhui Provincial Laboratory of Tea Plant Biology and Utilization Anhui Agricultural UniversityHefei 230036AnhuiChina

Subgroup 4(Sg4)members of the R2R3-MYB are generally known as negative regulators of the phenylpropanoid pathway in *** previous research showed that a R2R3-MYB Sg4 member from Camellia sinensis(CsMYB4a)inhibits expression of some genes in the phenylpropanoid pathway,but its physiological function in the tea plant remained ***,CsMYB4a was found to be highly expressed in anther and filaments,and participated in regulating filament *** analysis and exogenous auxin treatment showed that the target of CsMYB4a might be the auxin signal ***/indole-3-acetic acid 4(AUX/IAA4),a repressor in auxin signal transduction,was detected from a yeast two-hybrid screen using CsMYB4a as *** silencing assays showed that both CsIAA4 and CsMYB4a regulate filament *** plants overexpressing CsIAA4 were insensitive to exogenous a-NAA,consistent with overexpression of ***-protein interaction experiments revealed that CsMYB4a interacts with N-terminal of CsIAA4 to prevent CsIAA4 *** out of the endogenous NtIAA4 gene,a CsIAA4 homolog,in tobacco alleviated filament growth inhibition and a-NAA insensitivity in plants overexpressing *** results strongly suggest that CsMYB4a works synergistically with CsIAA4 and participates in regulation of the auxin pathway in stamen.

关键词： AUX/IAA4 Auxin signaling CsMYB4a Subgroup 4 R2R3-MYB

Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

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Science China(Life Sciences) 2024年第1期67卷 51-66页

作者： Yonghui jiang Xueying Gao yue Liu Xueqi Yan huangcong Shi Rusong Zhao Zi-jiang Chen Fei Gao Han Zhao Shigang Zhao Center for Reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250012China National Research Center of Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China The Affiliated Suzhou Hospital of Nanjing Medical University Suzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215000China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200127China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of ScienceBeijing 100101China

Obesity,which can arise from genetic or environmental factors,has been shown to cause serious damages to the reproductive *** ovary,as one of the primary regulators of female fertility,is a complex organ comprised of heterogeneous cell types that work together to maintain a normal ovarian microenvironment(OME).Despite its importance,the effect of obesity on the entire ovary remains poorly *** this study,we performed ovary single-cell and nanoscale spatial RNA sequencing to investigate how the OME changed under different kinds of obesity,including high-fat diet(HFD)induced obesity and Leptin ablation induced obesity(OB).Our results demonstrate that OB,but not HFD,dramatically altered the proportion of ovarian granulosa cells,theca-interstitial cells,luteal cells,and endothelial ***,based on the spatial dynamics of follicular development,we defined four subpopulations of granulosa cell and found that obesity drastically disrupted the differentiation of mural granulosa cells from small to large antral ***,HFD enhanced follicle-stimulating hormone(FSH)sensitivity and hormone conversion,while OB caused decreased sensitivity,inadequate steroid hormone conversion,and impaired follicular *** differences can be explained by the differential expression pattern of the transcription factor ***,our study provides a powerful and high-resolution resource for profiling obesity-induced OME and offers insights into the diverse effects of obesity on female reproductive disorders.

关键词： obesity ovarian microenvironment snRNA-seq Stereo-seq Foxo1

Probabilistic back-analysis of rainfall-induced landslides for slope reliability prediction with multi-source information

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第9期16卷 3575-3594页

作者： Shui-hua jiang Hong-Hu Jie Jiawei Xie Jinsong huang Chuang-Bing Zhou School of Infrastructure Engineering Nanchang UniversityXuefu Road 999Nanchang330031China Discipline of Civil Surveying and Environmental EngineeringFaculty of Engineering and Built EnvironmentThe University of NewcastleCallaghanNSW2308Australia

Probabilistic back-analysis is an important means to infer the statistics of uncertain soil parameters,making the slope reliability assessment closer to the engineering ***,multi-source information(including test data,monitored data,field observation and slope survival records)is rarely used in current probabilistic *** the probabilistic back-analysis of spatially varying soil parameters and slope reliability prediction under rainfalls by integrating multi-source information is a challenging task since thousands of random variables and high-dimensional likelihood function are usually *** this paper,a framework by integrating a modified Bayesian Updating with Subset simulation(mBUS)method with adaptive Conditional Sampling(aCS)algorithm is established for the probabilistic back-analysis of spatially varying soil parameters and slope reliability *** this framework,the high-dimensional probabilistic back-analysis problem can be easily tackled,and the multi-source information(*** pressure heads and slope survival records)can be fully used in the back-analysis.A real Taoyuan landslide case in Taiwan,China is investigated to illustrate the effectiveness and performance of the established *** findings show that the posterior knowledge of soil parameters obtained from the established framework is in good agreement with the field ***,the updated knowledge of soil parameters can be utilized to reliably predict the occurrence probability of a landslide caused by the heavy rainfall event on September 12,2004 or forecast the potential landslides under future rainfalls in the Fuhsing District of Taoyuan City,Taiwan,China.

关键词： Rainfall-induced landslide Spatial variability Probabilistic back-analysis Slope reliability analysis Bayesian updating

Surface quality investigation in high-speed dry milling of Ti-6Al-4V by using 2D ultrasonic-vibration-assisted milling platform

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Advances in Manufacturing 2024年第2期12卷 349-364页

作者： Jin Zhang Li Ling Qian-yue Wang Xue-Feng huang Xin-Zhen Kang Gui-Bao Tao hua-Jun Cao College of Mechanical and Vehicle Engineering Chongqing UniversityChongqing400044People’s Republic of China State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing400044People’s Republic of China Aerospace Research Institute of Materials and Processing Technology Beijing100076People’s Republic of China

Ultrasonic-vibration-assisted milling(UVAM)is an advanced method for the efficient and precise machining of difficult-to-machine materials in modern ***,the milling efficiency is limited because the ultrasonic vibration toolholder ER16 collet has a critical cutting ***,a 2D UVAM platform is built to ensure precision machining efficiency and improve the surface quality without changing the milling *** evaluate this 2D UVAM platform,ultrasonic-vibration-assisted high-speed dry milling(UVAHSDM)is performed to process a titanium alloy(Ti-6Al-4V)on the platform,and the milling temperature,surface roughness,and residual stresses are selected as the important indicators for performance *** results show that the intermittent cutting mechanism of UVAHSDM combined with the specific spindle speed,feed speed,and vibration amplitude can reduce the milling temperature and improve the texture of the machined *** with conventional milling,UVAHSDM reduces surface roughness and peak-groove surface profile values and extends the range of residual surface compressive stresses from−413.96 MPa to−600.18 *** excellent processing performance demonstrates the feasibility and validity of applying this 2D UVAM platform for investigating surface quality achieved under UVAHSDM.

关键词： 2D ultrasonic-vibration-assisted milling(UVAM)platform Ultrasonic-vibration-assisted high-speed dry milling(UVAHSDM) Milling temperature Surface roughness Residual stress

Electrocatalytic performance of CNTs/graphene composited rare earth phthalocyanines(M=La,Y,Yb,Sc)

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Journal of Rare Earths 2024年第2期42卷 323-333,I0004页

作者： Tingting jiang Caixia Ou Luyi Wang Jun Chen Sydorov Dmytro Qian Zhang Jintian Luo hua Wang jiangxi Key Laboratory of Power Batteries and Materials Faculty of Materials Metallurgy and ChemistryJiangxi University of Science and TechnologyGanzhou341000China Joint Department of Electrochemical Energy Systems Institute of Bioorganic Chemistry and PetrochemistryNational Academy of Sciences of Ukraine38A VernadskyAveKiev03142Ukraine Guangdong Jiana Energy Technology Co. Ltd.Qingyuan511500China

In this study,a class of rare earth composite sandwich phthalocyanines(MPcs,M=La,Y,Yb,Sc) were prepared and compounded with graphene and carbon nanotubes to obtain MPc/Gr and MPc/CNTs *** electrocatalytic behaviors of MPc/Gr and MPc/CNTs electrodes were further *** results show that the central rare earth metal has a large influence on the electrocatalytic *** the MPcs/Gr samples,ScPc with the smallest ionic radius and molecular size can be more uniformly dispersed in graphene,and the hydrogen precipitation overpotential of ScPc/Gr electrode is514 mV,corresponding to a Tafel slope of 148 mV/dec,with better electrocatalytic performance than other rare earth metal *** for the MPc/CNTs composites,LaPc,which has the largest ionic radius and molecular size,is more uniformly dispersed on the surface of CNTs,so that the LaPc/CNT electrode exhibits the best LSV performance with the smallest corresponding Tafel slope(176 mV/dec).The main reason is the different binding modes of MPcs molecules in Gr and *** rare earth phthalocyanine is combined with layered graphene,the smallest size of rare earth phthalocyanine(ScPc)is more easily embedded in the graphene layer,resulting in better homogeneity of the composite,larger effective contact area and better electrocatalytic *** contrast,when rare earth phthalocyanine is bound to carbon nanotubes in a tubular structure,it is mainly bound by attaching to the surface or being entangled by the carbon *** this case,the rare-earth phthalocyanine molecules(LaPc)with larger layer spacing can provide more contact area with CNTs,forming a more uniform and effective composite,which eventually provides more active sites and better electrocatalytic performance.

关键词： Electrocatalysis Rare earth phthalocyanines Graphene(Gr) Carbon nanotubes(CNTs) Composite

Enhancing growth, liver health, and bile acid metabolism of tilapia(Oreochromis niloticus) through combined cholesterol and bile acid supplementation in plant-based diets

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Animal Nutrition 2024年第2期17卷 335-346页

作者： Jiayuan jiang Xing Lu Lixue Dong Juan Tian Jianmin Zhang Zhongbao Guo Yongju Luo Zongbin Cui hua Wen Ming jiang College of Fisheries and Life Science Shanghai Ocean University Yangtze River Fisheries Research Institute Chinese Academy of Fishery Sciences Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application State Key Laboratory of Applied Microbiology Southern ChinaInstitute of MicrobiologyGuangdong Academy of Sciences Guangxi Institute of Fisheries

The present study aimed to compare the nutritional effects of cholesterol, bile acids, and combination of cholesterol with bile acids in plant-based diets on juvenile genetically improved farmed tilapia(GIFT;Oreochromis niloticus). The isonitrogenous(321 g/kg crude protein) and isolipidic(76 g/kg crude fat) diets(Con diet) were based on plant protein sources, which included corn gluten meal, soybean meal, cottonseed meal and rapeseed meal. The Con diet was supplemented with 12 g/kg cholesterol(CHO diet), 0.2 g/kg bile acids(BAs diet), a combination of 12 g/kg cholesterol and 0.2 g/kg bile acids(CHO-BAs diet),respectively. Each diet was fed to three tanks in an indoor recirculating aquaculture system for 9 *** showed that compared to the Con group, fish had a higher weight gain rate, hepatosomatic index,and a lower feed conversion ratio in the CHO-BAs group. The highest levels of whole-fish fat and ash were found in the Con group. Serum parameters, including activities of alanine aminotransferase(ALT) and aspartate transaminase(AST), along with levels of glucose(GLU) and triglyceride(TG) except for total cholesterol(TCHO), were lower in the CHO, BAs, and CHO-BAs groups than those in the Con group(P <0.001). Histological examination revealed that fish in the Con group exhibited severe hepatocyte vacuolization and diminished hepatocyte proliferation. Gene expression analysis indicated that the transcriptional levels of bile acid metabolism-related genes(including fxr, fgf19, bsep) were up-regulated in the CHO-BAs group(P < 0.05), whereas cholesterol metabolism-related genes(acly and hmgcr) were downregulated in both CHO and CHO-BAs groups(P < 0.001). Moreover, UPLC-MS/MS analysis revealed that the higher taurine-conjugated bile acids(T-BAs), followed by free bile acids(Free-BAs) and glycine(G-BAs)were determined in tilapia bile. Among these, taurochenodeoxycholic bile acid was the predominant bile acid. Dietary bile acids supplementation also increased the pr

关键词： Bile acid metabolism Tilapia Plant-based feed Cholesterol Liver health

Artificial intelligence enhances the management of esophageal squamous cell carcinoma in the precision oncology era

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World Journal of Gastroenterology 2024年第39期30卷 4267-4280页

作者： Wan-yue Zhang Yong-Jian Chang Rui-hua Shi School of Medicine Southeast UniversityNanjing 221000Jiangsu ProvinceChina School of Cyber Science and Engineering Southeast UniversityNanjing 210009Jiangsu ProvinceChina Department of Gastroenterology Zhongda HospitalSoutheast UniversityNanjing 210009Jiangsu ProvinceChina

Esophageal squamous cell carcinoma(ESCC)is the most common histological type of esophageal cancer with a poor *** diagnosis and prognosis assessment are crucial for improving the survival rate of ESCC *** the advancement of artificial intelligence(AI)technology and the proliferation of medical digital information,AI has demonstrated promising sensitivity and accuracy in assisting precise detection,treatment decision-making,and prognosis assessment of *** has become a unique opportunity to enhance comprehen-sive clinical management of ESCC in the era of precision *** review examines how AI is applied to the diagnosis,treatment,and prognosis assessment of ESCC in the era of precision oncology,and analyzes the challenges and potential opportunities that AI faces in clinical *** insights into future prospects,it is hoped that this review will contribute to the real-world application of AI in future clinical settings,ultimately alleviating the disease burden caused by ESCC.

关键词： Esophageal squamous cell carcinoma Artificial intelligence Deep learning Machine learning Precision tumor therapy

Healing the structural defects of spinel MnFe_(2)O_(4) to enhance the electrocatalytic activity for oxygen reduction reaction

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Journal of Energy Chemistry 2024年第10期97卷 12-19,I0001页

作者： Manting Tang yue Zou Zhiyong jiang Peiyu Ma Zhiyou Zhou Xiaodi Zhu Jun Bao Shi-Gang Sun National Synchrotron Radiation Laboratory University of Science and Technology of ChinaHefei 230026AnhuiChina State Key Laboratory of Physical Chemistry of Solid Surfaces Department of ChemistryCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 36005 FujianChina iChEM(Collaborative Innovation Center of Chemistry for Energy Materials) University of Science and Technology of ChinaHefei 230026AnhuiChina

Spinel metal oxides containing Mn,Co,or Fe(AB_(2)O_(4),A/B=Mn/Fe/Co)are one of the most promising nonPt electrocatalysts for oxygen reduction reaction(ORR)in alkaline ***,the low conductivity of metal oxides and the poor intrinsic activities of transition metal sites lead to unsatisfactory ORR *** this study,eutectic molten salt(EMS)treatment is employed to reconstruct the atomic arrangement of MnFe_(2)O_(4)electrocatalyst as a prototype for enhancing ORR *** analyses by using XAFS,soft XAS,XPS,and electrochemical methods reveal that the EMS treatment reduces the oxygen vacancies and spinel inverse in MnFe_(2)O_(4)effectively,which improves the electric conductivity and increases the population of more catalytically active Mn^(2+)sites with tetrahedral ***,the enhanced Mn-O interaction after EMS treatment is conducive to the adsorption and activation of O_(2),which promotes the first electron transfer step(generally considered as the ratedetermining step)of the ORR *** a result,the EMS treated MnFe_(2)O_(4)catalyst delivers a positive shift of 40 mV in the ORR half-wave potential and a two-fold enhanced mass/specific *** work provides a convenient approach to manipulate the atomic architecture and local electronic structure of spinel oxides as ORR electrocatalysts and a comprehensive understanding of the structureperformance relationship from the molecular/atomic scale.

关键词： Spinel MnFe_(2)O_(4) Oxygen reduction reaction Spinel inverse Oxygen vacancies Eutectic molten salt

An efficient data-driven global sensitivity analysis method of shale gas production through convolutional neural network

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Petroleum Science 2024年第4期21卷 2475-2484页

作者： Liang Xue Shuai Xu Jie Nie Ji Qin jiang-Xia Han yue-Tian Liu Qin-Zhuo Liao State Key Laboratory of Petroleum Resources and Engineering China University of Petroleum (Beijing) Department of Oil-Gas Field Development Engineering College of Petroleum Engineering China University of Petroleum (Beijing) Chuanqing Drilling Engineering Co.Ltd. China National Petroleum Corporation

The shale gas development process is complex in terms of its flow mechanisms and the accuracy of the production forecasting is influenced by geological parameters and engineering parameters. Therefore, to quantitatively evaluate the relative importance of model parameters on the production forecasting performance, sensitivity analysis of parameters is required. The parameters are ranked according to the sensitivity coefficients for the subsequent optimization scheme design. A data-driven global sensitivity analysis(GSA) method using convolutional neural networks(CNN) is proposed to identify the influencing parameters in shale gas production. The CNN is trained on a large dataset, validated against numerical simulations, and utilized as a surrogate model for efficient sensitivity analysis. Our approach integrates CNN with the Sobol' global sensitivity analysis method, presenting three key scenarios for sensitivity analysis: analysis of the production stage as a whole, analysis by fixed time intervals, and analysis by declining rate. The findings underscore the predominant influence of reservoir thickness and well length on shale gas production. Furthermore, the temporal sensitivity analysis reveals the dynamic shifts in parameter importance across the distinct production stages.

关键词： Shale gas Global sensitivity Convolutional neural network Data-driven