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Effect of gemcitabine on the expression of apoptosis-related genes in human pancreatic cancer cells

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World Journal of Gastroenterology 2006年第10期12卷 1597-1602页

作者： Pei-hong jiang Yoshiharu Motoo Norio Sawabu Toshinari Minamoto Department of Internal Medicine and Medical Oncology Cancer Research Institute Kanazawa University Kanazawa Japan Department of Medical Oncology Kanazawa Medical University Uchinada Ishikawa Japan Division of Diagnostic Molecular Oncology Cancer Research InstituteKanazawa University Kanazawa Japan

AIM： TO investigate the expression of genes involved in the gemcitabine-induced cytotoxicity in human pancreatic cancer cells. METHODS： A human pancreatic cancer cell line, PANC-1, was cultured. 1 × 10^4 PANG-1 cells were plated in 96-well microtiter plates. After being incubated for 24 h, gemcitabine was added to the medium at concentrations ranging 2.5 -1 000 mg/L. The AlamarBlue dye method was used for cell growth analysis. DNA fragmentation was quantitatively assayed using a DNA fragmentation enzyme-linked immunosorbent assay （ELISA） kit. PAP and TP53INP1 mRNA expression was determined using the reverse transcription-polymerase chain reaction with semi-quantitative analysis. The expression of GSK-3β and phospho-GSK-3β proteins was examined with Western blot analysis. RESULTS： The IC50 for the drug after a 48-h exposure to gemcitabine was 16 mg/L. The growth of PANC-1 cells was inhibited by gemcitabine in a concentration-dependent manner （P〈 0.0001） and the cell growth was also inhibited throughout the time course （P〈0.0001）. The DNA fragmentation rate in the gemcitabine-treated group at 48 h was 44.7 %, whereas it was 25.3 % in the untreated group. The PAP mRNA expression was decreased after being treal^l with gemcitabine, whereas the TP53INP1 mRNA was increased by the gemcitabine treatment. Western blot analysis showed that phospho- GSK-3βZ^ser9 was induced by the gemcitabine treatment. CONCLUSION： Gemcitabine suppresses PANC-1 cell proliferation and induces apoptosis. Apoptosis is considered to be associated with the inhibition of PAPand GSK-3β, and the activation of TP53INP1 and pospho- GSK-3β^ser9 .

关键词： Gemcitabine Pancreatitis-associated protein TPS3INP1 GSK-3β PANC-1

Video coding using geometry based block partitioning and reordering discrete cosine transform

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Journal of Zhejiang University-Science C(Computers and Electronics) 2012年第1期13卷 71-82页

作者： Yi-xiong ZHANG jiang-hong SHI Wei-dong WANG Department of Communication Engineering Xiamen University Xiamen 361005 China Department of information Science and Electronic Engineering Zhejiang University Hangzhou 310027 China

Geometry based block partitioning （GBP） has been shown to achieve better performance than the tree structure based block partitioning （TSBP） of H.264. However, the residual blocks of GBP mode after motion compensation still present some non-vertical/non-horizontal orientations, and the conventional discrete cosine transform （DCT） may generate many high-frequency coefficients. To solve this problem, in this paper we propose a video coding approach by using GBP and reordering DCT （RDCT） techniques. In the proposed approach, GBP is first applied to partition the macroblocks. Then, before performing DCT, a reordering operation is used to adjust the pixel positions of the residual macroblocks based on the partition information. In this way, the partition information of GBP can be used to represent the reordering information of RDCT, and the bitrate can be reduced. Experimental results show that, compared to H.264/AVC, the proposed method achieves on average 6.38% and 5.69% bitrate reductions at low and high bitrates, respectively.

关键词： Geometry based block partitioning Reordering DCT Motion compensation Video coding

C:N:P stoichiometry of perennial herbs’ organs in the alpine steppe of the northern Tibetan Plateau

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Journal of Mountain Science 2019年第9期16卷 2039-2047页

作者： MA Xing-xing hong jiang-tao WANG Xiao-dan College of Geographical Sciences Shanxi Normal University Linfen 041000 China Institute of Mountain Hazards and Environment Chinese Academy of Sciences Chengdu 610041 China

The patterns of C:N:P stoichiometry across ecosystems are important in understanding biogeochemical processes. The stoichiometry of nutrients at the leaf and root level have been reported previously, but relationships of other plant organs, such as stems and the reproductive organs, remain unclear. We collected 228 samples of leaves, roots, stems and reproductive organs from 11 common plant species at 25 sites on the Tibetan Plateau to explore the relationships of C:N:P stoichiometry both within and across plant organs. The average C concentrations in the roots, leaves, stems and reproductive organs were 427.32, 410.51, 421.11 and 416.72 mg g-1, respectively. The shoot tissues(leaves, stems and reproductive organs) had significantly higher N and P concentrations than the roots. The N and P concentrations had a significant positive correlation within the same organ. The nutrient concentrations(N and P) and nutrient ratios(C:N, C:P and N:P) were significantly correlated across all pairwise organ combinations. Our data suggest that alpine perennial herbs share similar evolutionary histories and have constrained patterns of covariation for C concentrations, with differential patterns for N and P stoichiometry across organs. Our data also indicate that covarying sets of nutrient traits are consistent across environments and biogeographical regions and demonstrate convergent evolution in plant nutritional characteristics in extreme alpine environments.

关键词： Shoot tissues Chemical elements Biogeochemical process Alpine steppe Tibet Plateau

Effects of Sc doping on electrical conductivity of BaZrO_3 protonic conductors

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Rare Metals 2012年第1期31卷 71-74页

作者： Guo, Ruisong Wu, Lijun Ren, Jianxun Zhang, Jianfang jiang, hong Key Laboratory of Advanced Ceramics and Machining Technology Ministry of Educatton Tianjin University Tianjin 300072 China No.53 Institute of China Ordnance Industry Group Jinan 250031 China Guizhou Broadcasting and Television College Guiyang 550004 China

BaZr1-xScxO3-0.5x (x=0.07,0.10,0.13,0.16) powders were prepared by solid-state reaction method,and ZnO was used as sintering *** with different amount of ZnO additive were sintered at 1450·C for 6 h in *** cubic perovskite phase proton conductors were *** was measured by electrochemical *** was shown that Sc doping could increase conductivity through enhancing the carrier concentration in the material,but excessive Sc content might decrease the carrier concentration because of its charge *** had an influence on carrier concentration and mobility and affected the electrical conductivity.2 mol% ZnO and 13 mol% ScO1.5 doped sample showed the highest DC conductivity of 3.6×10-3 S·cm-1 tested at 800·C in wet hydrogen atmosphere.

关键词： protonic conductor perovskite carrier concentration mobility

Characterization of retinal microvasculature in acute non-arteritic anterior ischemic optic neuropathy using the retinal functional imager: a prospective case series

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Eye and Vision 2019年第1期6卷 17-22页

作者： Amanda D.Henderson hong jiang Jianhua Wang Wilmer Eye Institute Johns Hopkins University School of Medicine600 N.Wolfe StreetWilmer 233BaltimoreMD 21231USA Bascom Palmer Eye Institute University of MiamiMiller School of Medicine900 NW 17th StreetMiamiFL 33136USA

Background:Non-arteritic anterior ischemic optic neuropathy(NAION)is the most common cause of acute optic neuropathy in patients over 50 years of age,and many affected individuals are left with permanent visual *** the frequency of NAION and its often devastating effects on vision,no effective treatment has been *** understanding of the acute vascular effects in NAION,using advanced ophthalmic imaging techniques like the retinal function imager,may shed light on potential treatment ***:Five patients with acute NAION underwent retinal functional imaging within 2 weeks of the onset of their visual symptoms,and at 1 month and 3 months after *** arteriolar and venular blood flow velocities were calculated for each eye at each time *** Wilcoxon rank sum test was used to compare blood flow velocity results with a normative ***:The average arteriolar blood flow velocity in the normative group was 3.8 mm/s,and the average venular blood flow velocity was 3.0 mm/s,versus 4.1 mm/s and 2.7 mm/s,respectively,in the NAION-affected group at *** arteriolar blood flow increased slightly to 4.2 mm/s one month after the acute NAION event,then decreased to 3.8 mm/s three months after the *** venular blood flow velocity was 2.8 mm/s 1 month after the NAION event and 2.7 mm/s 3 months after the *** in blood flow velocity between the NAION-affected and control groups were not statistically significant at any time point;however,there was a trend toward increasing blood flow velocity initially after an NAION,with a decrease over ***:This study demonstrates the feasibility of retinal function imaging to quantify macular blood flow velocity in patients with acute *** were no statistically significant differences in blood flow velocity detected between NAION-affected eyes and healthy controls at any of the time points examined;however,there was a trend toward an i

关键词： Retinal functional imager Non-arteritic anterior ischemic optic neuropathy Blood flow velocity

Variation in carbon,nitrogen and phosphorus partitioning between above-and belowground biomass along a precipitation gradient at Tibetan Plateau

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Journal of Mountain Science 2016年第4期13卷 661-671页

作者： hong jiang-tao WANG Xiao-dan WU Jian-bo Institute of Mountain Hazards and Environment Academy of Sciences of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

Precipitation is a potential factor that significantly affects plant nutrient pools by influencing biomass sizes and nutrient concentrations. However, few studies have explicitly dissected carbon(C), nitrogen(N) and phosphorus(P) pools between above- and belowground biomass at the community level along a precipitation gradient. We conducted a transect(approx. 1300 km long) study of Stipa purpurea community in alpine steppe on the Tibet Plateau of China to test the variation of N pool of aboveground biomass/N pool of belowground biomass(AB/BB N) and P pool of aboveground biomass/P pool of belowground biomass(AB/BB P) along a precipitation gradient. The proportion of aboveground biomass decreased significantly from mesic to drier sites. Along the belt transect, the plant N concentration was relatively stable; thus, AB/BB N increased with moisture due to the major influences by above- and belowground biomass allocation. However, P concentration of aboveground biomass decreased significantly with increasing precipitation and AB/BB P did not vary with aridity because of the offset effect of the P concentration and biomass allocation. Precipitation gradients do decouple the N and P pool of a S. purpurea community along a precipitation gradient in alpine steppe. The decreasing of N:P in aboveground biomass in drier regions may indicate much stronger N limitation in more arid area.

关键词： Biomass allocation Nutrient concentration Qinghai-Tibetan Plateau Alpine steppe Stipa purpurea

Scalable connection-based flow control scheme for application-specific network-on-chip

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The Journal of China Universities of Posts and Telecommunications 2011年第6期18卷 98-105页

作者： LIN Shi-jun SHI jiang-hong CHEN Hui-huang School of Information Science and Technology Xiamen University Xiamen 361005 China

In this paper, a scalable connection-based flow control scheme is proposed for application-specific network-on-chip （NoC）. The proposed scheme exploits two distinctive characteristics of NoC, namely traffic predictability and abundant wire resource, to achieve significant performance enhancements. First, the burst injection data are regulated into constant data streams and a connection-based method is used to ensure that all links are not overloaded at any time. Consequently, the number of packets in the network is decreased, leading to a reduced congestion probability and improved communication performance. Second, a simple architecture of the central controller is proposed to guarantee that the proposed scheme has small area overhead and is scalable. Simulation results show that compared with traditional switch-to-switch （STS） flow control scheme and pre-allocation based flow control schemes, the proposed scheme greatly increases the throughput and cuts down the average latency with reasonable area and energy overhead.

关键词： network-on-chip (NoC) system-on-chip (SoC) flow control

Inter-visit measurement variability of conjunctival vasculature and circulation in habitual contact lens wearers and non-lens wearers

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Eye and Vision 2019年第1期6卷 74-80页

作者： Jianhua Wang Liang Hu Ce Shi hong jiang Miller School of Medicine Bascom Palmer Eye InstituteUniversity of Miami1638 NW 10th AvenueMcKnight Building-Room 202AMiamiFL 33136USA School of Ophthalmology and Optometry Wenzhou Medical UniversityWenzhouZhejiangChina

Background:The inter-visit variation of measuring bulbar conjunctival microvasculature and microcirculation needs to be considered when the results from multiple visits are *** study examined the inter-visit variability of measuring conjunctival microvasculature and microcirculation in habitual contact lens(HCL)wearers and non-contact lens(NCL)***:Twenty-eight subjects were recruited including 13 HCL wearers(10 females and 3 males;mean age±standard deviation,25.8±4.6 years)who had worn contact lenses on a daily basis for at least 3 years and 15 NCL wearers(10 females and 5 males,age 25.5±4.0 years)were *** temporal bulbar conjunctiva was imaged using a functional slit-lamp bio-microscope(FSLB)imaging *** imaging was performed in the morning when the HCL wearers did not wear their *** measurements included conjunctival vessel diameter,vessel density,blood flow velocity and flow *** addition,conjunctival microvasculature was analyzed using monofractal(Dbox,representing vessel density)and multifractal(D0 representing vessel complexity)*** repeated measurement was conducted at least one week after the first visit and both eyes of each participant were *** coefficient of variation(CV)was calculated as the standard deviation of the differences between test and re-test then divided by the mean of the *** intra-class correlation coefficient(ICC)was also ***:No significant differences of all vascular measurements in both the right and left eyes were found between two groups(P>0.05).Between two measurements on two different visits,the CV was from 2.4%(vessel density D0)to 63.5%(blood flow volume Q)in HCL wearers and from 3.4%(D0)to 40.6%(blood flow volume)in NCL *** ICC was from 0.60(vessel diameter)to 0.81(axial blood flow velocity VA)in HCL wearers and from 0.44(Q)to 0.68(cross-sectional blood flow velocity VS)in NCL ***:The measurement variability of

关键词： Variability Bulbar conjunctiva Blood flow velocity Microvascular network Functional slit-lamp biomicroscopy(FSLB) Hemodynamics

Relationships between Chronic Diseases and Depression among Middleaged and Elderly People in China: A Prospective Study from CHARLS

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Current Medical Science 2020年第5期40卷 858-870页

作者： Chun-hong jiang Feng ZHU Ting-ting QIN School of Management Hubei University of EducationWuhan 430205China Department of Biliary-Pancreatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China

Given the rapid increase in the prevalence of chronic diseases in aging populations,this prospective study including 17707 adults aged≥45 years from China Health and Retirement Longitudinal Study was used to estimate the associations between chronic discase,multimorbidity,and depression among middle-aged and elderly adults in China,and explore the mediating *** symptoms were assessed using the 10-item Centre for Epidemiological Studies Depression Scale(CES-D-10)*** chronic physical conditions,including hypertension,diabetes,dyslipidemia,cancer,chronic lung disease,liver disease,heart failure,stroke,kidney disease,arthritis or rheumatism,asthma,digestive disease were *** prevalence rates for physical multimorbidity and depression(CES-D-10≥10)were 43.23%and 36.62%,*** multivariable logistic models and generalized estimating equation(GEE)models,we found all 12 chronic physical conditions,and multimorbidity were significantly associated with *** mobility problems and chronic pain explained more than 30%of the association for all chronic conditions,with particularly high percentages for stroke(51.56%)and cancer(51.06%)in mobility problems and cancer(53.35%)in chronic *** activities of daily living(ADL)explained 34.60%of the stroke-cancer relationship,while sleep problems explained between 10.15%(stroke)and 14.89%(chronic lung disease)of the *** with chronic diseases or multimorbidity are significantly more likely to be *** symptoms involving limitations of ADL and mobility difficulties mediated much of the association between chronic diseases and incident *** symptoms could be targeted for interventions to ameliorate the incidence of depression among individuals with chronic conditions.

关键词： chronic disease multimorbidity depression functional symptoms mediation effect