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Protection Against Hepatic Ischemia-reperfusion Injury in Rats by Oral Pretreatment With Quercetin

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Biomedical and Environmental Sciences 2003年第1期16卷 1-8页

作者： JUN-feng SU, CHANG-jiang GUO , JING-YU WEI, JI-JUN YANG, YU-GANG jiang, AND yun-feng LIDepartment of Nutrition, Institute of Hygiene and Environmental Medicine, Tianjin 300050, China Department of Nutrition Institute of Hygiene and Environmental MedicineTianjin 天津 300050

Objective To investigate the possible protection provided by oral quercetin pretreatment against hepatic ischemia-reperfusion injury in rats. Methods The quercetin （0.13 mmol/kg） was orally administrated in 50 min prior to hepatic ischemia-reperfusion injury. Ascorbic acid was also similarly administered. The hepatic content of quercetin was assayed by high performance liquid chromatography （HPLC）. Plasma glutamic pyruvic transaminase （GPT）, glutamic oxaloacetic transaminase （GOT） activities and malondialdehyde （MDA） concentration were measured as markers of hepatic ischemia-reperfusion injury. Meanwhile, hepatic content of glutathione （GSH）, activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px） and xanthine oxidase （XO）, total antioxidant capacity （TAOC）, contents of reactive oxygen species （ROS） and MDA, DNA fragmentation were also determined. Results Hepatic content of quercetin after intragastric administration of quercetin was increased significantly. The increases in plasma GPT

关键词： Quercetin Ischemia-reperfusion injury Lipid peroxidation Liver

First total synthesis of (±)-puyanin and (±)-4'-O-methylbonannione

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Chinese Chemical Letters 2010年第11期21卷 1267-1269页

作者： Jin Hui Yang Yu Heng Zhang Hong Jun Li Shi Zhi jiang yun feng Li Key Laboratory of Energy Sources and Chemical Engineering of Ningxia Ningxia University Yinchuan 750021 China

A facile approach for the first total synthesis of two naturally occurring geranylated flavonoids,（±）-puyanin 1 and（±）-4＇-O- methylbonannione 2 has been obtained with total yield 27%and 21%,*** key steps were regioselective cyclization of geranylated trihydroxychalcone and regioselective geranylation of 2,4,6-trihydroxyacetophenone.

关键词： Puyanin 4'-O-Methylbonannione Flavanone Geranylated flavonoids Total synthesis

Identification and validation of novel loci associated with wheat quality through a genome-wide association study

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Journal of Integrative Agriculture 2022年第11期21卷 3131-3147页

作者： PU Zhi-en YE Xue-ling LI Yang SHI Bing-xin GUO Zhu DAI Shou-fen MA Jian LIU Ze-hou jiang yun-feng LI Wei jiang Qian-tao CHEN Guo-yue WEI Yu-ming ZHENG You-liang College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China Triticeae Research Institute Sichuan Agricultural UniversityWenjiang 611130P.R.China Key Laboratory of Coarse Cereal Processing Ministry of Agriculture and Rural Affairs/College of Food and Biological EngineeringChengdu UniversityChengdu 610106P.R.China Crop Research Institute Sichuan Academy of Agricultural SciencesChengdu 610066P.R.China

Understanding the genetic basis of quality-related traits contributes to the improvement of grain protein concentration(GPC),grain starch concentration(GSC),and wet gluten concentration(WGC)in *** this study,a genome-wide association study(GWAS)based on a mixed linear model(MLM)was performed on 236 wheat accessions,including 160 cultivars and 76 landraces,using a 55K single nucleotide polymorphism(SNP)array in multiple environments.A total of 12 stable QTL/SNPs that control different quality traits in this populations in at least two environments under stripe rust stress were *** these 12,three,seven and two QTLs associated with GPC,GSC and WGC were characterized,respectively,and they were located on chromosomes(chr)1B,1D,2A,2B,2D,3B,3D,5D,and 7D with the phenotypic variation explained(PVE)ranging from 4.2 to 10.7%.Compared with the previously reported QTLs/genes,five QTLs(***-1BL,***-1DS,***-2DL.1,***-2DL.2,and ***-5DL)were potentially *** markers for the SNPs AX-108770574 and AX-108791420 on chr5D associated with wet gluten concentration were successfully *** phenotypes of the cultivars containing the A-allele in AX-108770574and the T-allele in AX-108791420 were extremely significantly(P<0.01)higher than those of the landraces containing the G-or C-allele with respect to the wet gluten concentration in each of the *** KASP markers developed and validated in this study could be utilized in molecular breeding aimed at improving the quality of wheat.

关键词： cultivars landraces grain protein concentration(GPC) grain starch concentration(GSC) wet gluten concentration(WGC) 55K SNP validation

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基于动网格的脉冲爆震发动机性能研究

推进技术 2010年第5期31卷 629-633页

作者：姜云峰王云雷郭昆北京航空航天大学自动化科学与电气工程学院北京100191 北京动力机械研究所北京100074

为了获得以工程应用为背景的脉冲爆震发动机(PDE)模型可靠的性能评估方法,应用数值模拟与经验数据相结合的方法计算了某多管旋转阀PDE的性能。结果显示:采用动网格技术可以获得冷态填充过程中发动机的充填系数;结合煤油爆震的数据得到... 详细信息

为了获得以工程应用为背景的脉冲爆震发动机(PDE)模型可靠的性能评估方法,应用数值模拟与经验数据相结合的方法计算了某多管旋转阀PDE的性能。结果显示:采用动网格技术可以获得冷态填充过程中发动机的充填系数;结合煤油爆震的数据得到所设计的六管旋转阀PDE的平均净推力为827.8N。该方法融合了CFD计算和试验数据,为今后PDE性能估算提供了很好的参考。

关键词：脉动式喷气发动机动网格净推力性能研究

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脉冲爆震发动机数值模拟

推进技术 2010年第6期31卷 783-788页

作者：姜云峰刘建文北京航空航天大学自动化科学与电气工程学院北京100191 北京动力机械研究所北京100074

为了获得脉冲爆震发动机(PDE)工作过程数值模拟技术,发展了DET2D程序,并以美国海军研究生院的气动阀式PDE为研究对象,进行了整机数值模拟。结果显示:所发展程序稳定、高效,可以用于多脉冲PDE流场的数值模拟及吸气式PDE数值模拟。通过计... 详细信息

为了获得脉冲爆震发动机(PDE)工作过程数值模拟技术,发展了DET2D程序,并以美国海军研究生院的气动阀式PDE为研究对象,进行了整机数值模拟。结果显示:所发展程序稳定、高效,可以用于多脉冲PDE流场的数值模拟及吸气式PDE数值模拟。通过计算,验证了PDE的工作原理和所发展程序的正确性。

关键词：脉冲爆震发动机数值仿真二维

A Model of Competition with Environmental Taxes and Regulation Under Conditions of Oligopoly

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Systems Science and Systems Engineering 2002年第3期11卷 275-279页

作者： XIAO jiang-wen, LUO yun-feng, ZHAO Yong, YUE Chao-yuanInstitute of Systems Engineering, Huazhong University of Science and Technology, Wuhan 430074, China Institute of Systems Engineering Huazhong University of Science and Technology Wuhan 430074 China

The Cournot static game with complete information is reviewed. A model of competition with environmental taxes under conditions of oligopoly is built based on the Cournot game, and some helpful conclusions are drawn from the model. A game model with regulation of government is also established. Finally the optimization problem of market structure is discussed.

关键词： game theory Cournot model oligopoly tax regulation

Effect of high-molecular-weight glutenin subunit Dy10 on wheat dough properties and end-use quality

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Journal of Integrative Agriculture 2023年第6期22卷 1609-1617页

作者： WANG Yan GUO Zhen-ru CHEN Qing LI Yang ZHAO Kan WAN Yong-fang Malcolm JHAWKESFORD jiang yun-feng KONG Li PU Zhi-en DENG Mei jiang Qian-tao LAN Xiu-jin WANG Ji-rui CHEN Guo-yue MA Jian ZHENG You-liang WEI Yu-ming QI Peng-fei State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130P.R.China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130P.R.China Rothamsted Research Harpenden AL52JQUK

High-molecular-weight glutenin subunits(HMW-GSs) are the most critical grain storage proteins that determine the unique processing qualities of wheat. Although it is a part of the superior HMW-GS pair(Dx5+Dy10), the contribution of the Dy10 subunit to wheat processing quality remains unclear. In this study, we elucidated the effect of Dy10 on wheat processing quality by generating and analyzing a deletion mutant(with the Dy10-null allele), and by elucidating the changes to wheat flour following the incorporation of purified Dy10. The Dy10-null allele was transcribed normally,but the Dy10 subunit was lacking. These findings implied that the Dy10-null allele reduced the glutenin:gliadin ratio and negatively affected dough strength(i.e., Zeleny sedimentation value, gluten index, and dough development and stability times) and the bread-making quality;however, it positively affected the biscuit-making quality. The incorporation of various amounts of purified Dy10 into wheat flour had a detrimental effect on biscuit-making quality. The results of this study demonstrate that the Dy10 subunit is essential for maintaining wheat dough strength. Furthermore, the Dy10-null allele may be exploited by soft wheat breeding programs.

关键词： HMW-GS nonsense mutation Dy10-null allele end-use quality

Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments

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Journal of Integrative Agriculture 2021年第11期20卷 2849-2861页

作者： LIU Hang TANG Hua-ping LUO Wei MU Yang jiang Qian-tao LIU Ya-xi CHEN Guo-yue WANG Ji-rui ZHENG Zhi QI Peng-fei jiang yun-feng CUI Fa SONG Yin-ming YAN Gui-jun WEI Yu-ming LAN Xiu-jin ZHENG You-liang MA Jian State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Ministry of Science and Technology/Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China Agriculture and Food CSIROSt LuciaBrisbane 4217Australia College of Agriculture Ludong UniversityYantai 264000P.R.China College of Agronomy and Biotechnology China Agricultural UniversityBeijing 100093P.R.China School of Plant Biology The University of Western AustraliaPerth WA 6009Australia

Uppermost-internode diameter(UID)is a key morphological trait associated with spike development and yield potential in *** understanding of its genetic basis remains largely ***,quantitative trait loci(QTLs)for UID with high-density genetic maps were identified in five wheat recombinant inbred line(RIL)*** total,25 QTLs for UID were detected in five RIL populations,and they were located on chromosomes 1A,1D(3 QTL),2B(2),2D(3),3B,3D,4A,4B(3),4D,5A(5),5B(2),6B,and *** them,five major and stable QTLs(***-2CN-1D.1,***-2SY-1D,***-QZ-2D,***-SC-3D,and ***-AS-4 B)were identified from each of the five RIL populations in multiple ***-2CN-1D.1,***-2SY-1D and ***-SC-3D are novel *** Allele Specific PCR(KASP)markers tightly linked to them were further investigated for developing near-isogenic lines(NILs)carrying the major ***,candidate genes at these intervals harboring major and stable QTLs were predicted,and they were associated with plant development and water transportation in most *** of physical locations of the identified QTL on the‘Chinese Spring’reference genome showed that several QTLs including two major ones,***-2CN-1D.1 and ***-2 SY-1 D,are likely allelic confirming their validity and *** significant relationships detected between UID and other agronomic traits and a proper UID were ***,our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.

关键词： uppermost-internode diameter wheat yield potential genetic basis quantitative trait loci

A major and stable QTL for wheat spikelet number per spike validated in different genetic backgrounds

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Journal of Integrative Agriculture 2022年第6期21卷 1551-1562页

作者： DING Pu-yang MO Zi-qiang TANG Hua-ping MU Yang DENG Mei jiang Qian-tao LIU Ya-xi CHEN Guang-deng CHEN Guo-yue WANG Ji-rui LI Wei QI Peng-fei jiang yun-feng KANG Hou-yang YAN Gui-jun WEI Yu-ming ZHENG You-liang LAN Xiu-jin MA Jian State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Ministry of Science and Technology/Triticeae Research InstituteSichuan Agricultural UniversityChengdu 611130P.R.China College of Resources Sichuan Agricultural UniversityChengdu 611130P.R.China College of Agronomy Sichuan Agricultural UniversityChengdu 611130P.R.China UWA School of Agriculture and Environment and The UWA Institute of Agriculture Faculty of ScienceUniversity of Western AustraliaCrawley 6009Australia

The spikelet number per spike(SNS)contributes greatly to grain yield in *** various genes that control wheat SNS is vital for yield *** study used a recombinant inbred line population genotyped by the Wheat55K single-nucleotide polymorphism array to identify two major and stably expressed quantitative trait loci(QTLs)for *** of them(***-2SY-2D.1)was reported previously,while the other(***-2SY-7A)was newly detected and further analyzed in this ***-2SY-7A had a high LOD value ranging from 4.46 to 16.00 and explained 10.21-40.78%of the phenotypic ***-2SY-7A was flanked by the markers AX-110518554 and AX-110094527 in a 4.75-cM interval on chromosome arm *** contributions and interactions of both major QTLs were further analyzed and *** effect of ***-2SY-7A was successfully validated by developing a tightly linked kompetitive allele specific PCR marker in an F_(2:3) population and a panel of 101 high-generation breeding wheat ***,several genes including the previously reported WHEAT ORTHOLOG OF APO1(WAPO1),an ortholog of the rice gene ABERRANT PANICLE ORGANIZATION 1(APO1)related to SNS,were predicted in the interval of *** summary,these results revealed the genetic basis of the multi-spikelet genotype of wheat line 20828 and will facilitate subsequent fine mapping and breeding utilization of the major QTLs.

关键词： yield potential QTL detection QTL validation predicted genes tightly linked KASP marker