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Sulfur-deficient Co9S8/Ni3S2 nanoflakes anchored on N-doped graphene nanotubes as high-performance electrode materials for asymmetric supercapacitors

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Science China(Technological Sciences) 2020年第4期63卷 675-685页

作者： ZHAO Jian SONG GuanYing YUAN XiangCheng SHEN Tong jiang qingyan MENG ALan LIN YuSheng LI Zhenjiang LI QingDang Key Laboratory of Polymer Material Advanced Manufacturing Technology of Shandong Provincial College of Electromechanical EngineeringCollege of Sino-German Science and TechnologyQingdao University of Science and TechnologyQingdao 266061China State Key Laboratory Base of Eco-chemical Engineering College of Chemistry and Molecular EngineeringCollege of Materials Science and EngineeringQingdao University of Science and TechnologyQingdao 266042China Ocean College Zhejiang UniversityZhoushan 316021China

In this paper,Co9S8/Ni3S2 nanoflakes(NFs)with sulfur deficiencies were grown in-situ on N-doped graphene nanotubes(NGNTs).They were successfully prepared through electrodeposition followed by hydrogenation treatment,which is able to act as a self-supported electrode for asymmetric supercapacitors(ASCs).Combining the defect-rich active materials with highly conductive skeletons,the hybrid electrode N-GNTs@sd-Co9S8/Ni3S2NFs show ultrahigh specific capacity of^304 mA hg^-1 and prominent rate capability(capacity retention ratio of^85%even at 100 Ag^-1),and deliver a long cycling lifespan of^1.9%capacitance loss after 10000 *** addition,an ASC was constructed using the as-synthesized composite electrode as the positive electrode and active carbon(AC)as the negative *** fabricated device shows a high energy density of^45.1 Wh kg^-1 at^3.4 kW kg^-1 and superior cycling *** work substantiates a smart strategy to fabricate novel composite electrode materials for next-generation supercapacitors by incorporating riched deficiencies into nanostructures.

关键词： sulfur-deficient Co9S8/Ni3S2NFs N-doped graphene nanotubes ultrahigh specific capacity prominent rate performance asymmetric supercapacitors

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Transcriptome analysis of adipose tissue and muscle of Laiwu and Duroc pigs

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Animal Nutrition 2024年第2期17卷 134-143页

作者： Jie Wu Fangyuan Yu Zhaoyang Di Liwen Bian Jie Yang Lina Wang qingyan jiang Yulong Yin Lin Zhang National Engineering Research Center for Breeding Swine Industry State Key Laboratory of Swine and Poultry Breeding IndustryGuangdong Laboratory of Lingnan Modern AgricultureGuangdong Provincial Key Laboratory of Animal Nutrition ControlCollege of Animal ScienceSouth China Agricultural University Key Laboratory of Agro-Ecological Processes in Subtropical Region Laboratory of Animal Nutritional Physiology and Metabolic ProcessInstitute of Subtropical AgricultureChinese Academy of Sciences

Fat content is an important trait in pig production. Adipose tissue and muscle are important sites for fat deposition and affect production efficiency and quality. To regulate the fat content in these tissues, we need to understand the mechanisms behind fat deposition. Laiwu pigs, a Chinese indigenous breed, have significantly higher fat content in both adipose tissue and muscle than commercial breeds such as *** this study, we analyzed the transcriptomes in adipose tissue and muscle of 21-d-old Laiwu and Duroc piglets. Results showed that there were 828 and 671 differentially expressed genes(DEG) in subcutaneous adipose tissue(SAT) and visceral adipose tissue(VAT), respectively. Functional enrichment analysis showed that these DEG were enriched in metabolic pathways, especially carbohydrate and lipid metabolism. Additionally, in the longissimus muscle(LM) and psoas muscle(PM), 312 and 335 DEG were identified, demonstrating enrichment in the cell cycle and metabolic pathways. The protein-protein interaction(PPI) networks of these DEG were analyzed and potential hub genes were identified, such as FBP1 and SCD in adipose tissues and RRM2 and GADL1 in muscles. Meanwhile, results showed that there were common DEG between adipose tissue and muscle, such as LDHB, THRSP, and DGAT2. These findings showed that there are significant differences in the transcriptomes of the adipose tissue and muscle between Laiwu and Duroc piglets(P < 0.05), especially in metabolic patterns. This insight serves to advance our comprehensive understanding of metabolic regulation in these tissues and provide targets for fat content regulation.

关键词： Laiwu Duroc Adipose Muscle Transcriptome Carbohydrate and lipid metabolism

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Astragalus polysaccharide enhances chickens immunity and inhibits H9N2 avian influenza virus in vitro and in vivo

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畜牧与兽医 2012年第S1期44卷 261-262页

作者： Kallon Sanpha Xiaorong Li Qianyun Xi qingyan jiang Yongliang Zhang Qingmei Xie College of Animal Science South China Agricultural University

This study was carried out to determine the immunization potential of astragalus polysaccharide(APS)against H9N2 avian influenza *** effect of APS on H9N2 in Chick embryo fibroblast(CEF)was determined using MTT *** expression of MHCⅠ,MHCⅡ,IFN-γ,IL-2,IL-4 and IL-10 were analyzed by real-time PCR in *** results showed that viral inhibition rate was significantly high(P<0.05),especially at 1250μg/ml-39.163 ug/*** cytokine

关键词： avian immunity Astragalus astragalus cytokine vaccine embryo blank pathogen killed

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Effects of “Bioactive” amino acids leucine, glutamate, arginine and tryptophan on feed intake and mRNA expression of relative neuropeptides in broiler chicks

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Journal of Animal Science and Biotechnology 2012年第4期3卷 199-206页

作者： Songbo Wang Paul Khondowe Shengfeng Chen Jianjian Yu Gang Shu Xiaotong Zhu Lina Wang Ping Gao Qianyun Xi Yongliang Zhang qingyan jiang College of Animal Sciences South China Agricultural University School of Natural Sciences Department of Biological SciencesUniversity of Zambia

Feed intake control is vital to ensuring optimal nutrition and achieving full potential for growth and development in poultry. The aim of the present study was to investigate the effects of L-leucine, L-glutamate, L-tryptophan and L-arginine on feed intake and the mRNA expression levels of hypothalamic Neuropeptide involved in feed intake regulation in broiler chicks. Leucine, glutamate, tryptophan or arginine was intra-cerebroventricularly （ICV） administrated to 4d-old broiler chicks respectively and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of Neuropeptide Y （NPY）, agouti related protein （AgRP）, pro-opiomelanocortin （POMC）, melanocortin receptor 4 （MC4R） and corticotrophin releasing factor （CRF）. Our results showed that ICV administration of L-leucine （0.15 or 1.5 μmol） significantly （P〈0.05） increased feed intake up to 2 h post-administration period and elevated both hypothalamic NPY and AgRP mRNA expression levels. In contrast, ICV administration of L-glutamate （1.6 μmol） significantly （P 〈 0.05） decreased feed intake 0.25, 0.5 and 2 h post-injection, and increased hypothalamic CRF and MC4R mRNA expression levels. Meanwhile, both L-tryptophan （10 or 100 μg） and L-arginine （20 or 200 μg） had no significant effect on feed intake. These findings suggested that L-leucine and L-glutamate could act within the hypothalamus to influence food intake, and that both orexigenic and anorexigenic Neuropeptide genes might contribute directly to these effects.

关键词： Broiler chicks Feed intake Hypothalamus Intracerebroventricular (ICY) L-leucine L-glutamate

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Effect of diacylglycerol acyltransferase 2overexpression in 3T3-L1 is associated to an increase in mono-unsaturated fatty acid accumulation

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Journal of Animal Science and Biotechnology 2014年第4期5卷 406-413页

作者： Zhiqi Zhang Gang Shu Xiaotong Zhu Junming Guo Han Cai Songbo Wang Lina Wang Ping Gao Qianyun Xi Yongliang Zhang Li Yuan qingyan jiang ALLTECH-SCAU Animal Nutrition Control Research Alliance College of Animal ScienceSouth China Agricultural University College of life science Xiamen University

Background：Fatty acid（FA） composition is the most important parameter affecting the flavor and nutritional value of the *** final and the only committed step in the biosynthesis of triglycerides is catalyzed by diacylglycerol acyltransferase 2（DGAT2）.The role of DGAT2 in lipid accumulation has been demonstrated in adipocytes,However,little is known about the effect of DGAT2 on the FA composition of these ***：To investigate the role of DGAT2 in regulating lipid accumulation,FA composition and the expression of adipogenic genes,we cloned the open reading frame of the porcine DGAT2 gene and established 3T3-L1 cells that overexpressed *** were then cultured in differentiation medium（DM） without FA,with a mixture of FAs（FA-DM）,or containing a ^（13）C stable isotope-labeled FA mixture（IFA-DM）.The FA composition of adipocytes was analyzed by gas chromatography-mass spectrometry and gas chromatography-isotope ratio mass *** PCR and western blotting were employed to detect expression of adipogenic genes in 3T3-L1 adipocytes cultured with FA-DM for 12 ***：The triacylglyceride（TAG） content was significantly higher in 3T3-L1 adipocytes overexpressing DGAT2 than in control *** cultured in DM or FA-DM for 12 d,cells overexpressing DGAT2 showed a higher proportion of unsaturated FAs（C16：1 and C18：1）.However,when cells overexpressing DGAT2 were cultured with FA-DM for30 min,the FA composition was almost identical to that of ***,the proportion of stable isotope-labeled FAs were similar in 3T3-L1 adipocytes overexpressing DGAT2 and control cells cultured in IFA-DM for 12 *** results collectively indicate that the higher proportion of mono-unsaturated FAs,C16：1 and C18：1,may originate from de novo FA synthesis but not from the uptake of specific FAs from the *** hypothesis is further supported by evidence that both mRNA and protein expression of genes involved in FA synthesis（ACACA,FASN,SCD1,and A-FABP）w

关键词： DGAT2 Fatty acid composition Overexpressing Pig

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Tauroursodeoxycholic acid(TUDCA)improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets

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Journal of Animal Science and Biotechnology 2022年第6期13卷 1917-1931页

作者： Min Song Fenglin Zhang Yiming Fu Xin Yi Shengchun Feng Zhichang Liu Dun Deng Qiang Yang Miao Yu Canjun Zhu Xiaotong Zhu Lina Wang Ping Gao Gang Shu Xianyong Ma qingyan jiang Songbo Wang Guangdong Provincial Key Laboratory of Animal Nutrition Control ALLT ECH-SCAU Animal Nutrition Control Research Allianceand National Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642P.R.China Institute of Animal Science Guangdong Academy of Agricultural SciencesState Key Laboratory of Livestock and Poultry BreedingKey Laboratory of Animal Nutrition and Feed Science in South ChinaMinistry of Agriculture and Rural AffairsGuangdong Key Laboratory of Animal Breeding and NutritionGuangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and EvaluationGuangzhou 510640P.R.China Maoming Branch Guangdong Laboratory for Lingnan Modern Agricultural Science and TechnologyMaoming 525000P.R.China

Background:Tauroursodeoxycholic acid(TUDCA),a hydrophilic bile acid,is the main medicinal component of bear bile and is commonly used to treat a variety of hepatobiliary ***,TUDCA has been shown to modulate the intestinal barrier function and alleviate DSS-induced colitis in ***,the effect of TUDCA on the intestinal barrier of weaned piglets remains largely ***:The weaned piglets and porcine IPEC-J2 intestinal epithelial cells were used to investigate the effects of TUDCA on intestinal barrier function in weaned piglets and explore the possible underlying *** vivo,72 healthy weaned piglets were randomly allocated into 2 groups according to their gender and body weight,and piglets were fed the basal diet with 0(control,CON)and 200 mg/kg TUDCA for 30 d,*** female and three male piglets reflecting the average bodyweight were slaughtered in each group and samples were *** vitro,IPEC-J2 cells were subjected to 100μmol/L TUDCA to explore the possible underlying ***:Our results demonstrated that dietary TUDCA supplementation significantly reduced the diarrhea incidence of weaned piglets,possibly attributing to the TUDCA-enhanced intestinal barrier function and *** addition,TUDCA supplementation altered serum metabolites and the relative abundance of certain gut bacteria,which might contribute to the improved intestinal barrier ***,the in-vitro results showed that TUDCA improved the ***-induced epithelial barrier impairment of IPEC-J2 cells and increased Takeda G-coupled protein receptor 5(TGR5)protein ***,knockdown of TGR5 and inhibition of myosin light chain kinase(MLCK)pathway abolished the TUDCA-improved epithelial barrier impairment in ***-treated IPEC-J2 cells,indicating the involvement of TGR5-MLCK in this ***:These findings showed that TUDCA improved intestinal barrier function associated with TGR5-MLCK pathway and the al

关键词： Gut bacteria Intestinal barrier function Serum metabolites Tauroursodeoxycholic acid(TUDCA) TGR5-MLCK pathway Weaned piglets

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Dietary chenodeoxycholic acid improves growth performance and intestinal health by altering serum metabolic profiles and gut bacteria in weaned piglets

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Animal Nutrition 2021年第2期7卷 365-375页

作者： Min Song Fenglin Zhang Lin Chen Qiang Yang Han Su Xiaohua Yang Haiwen He Mingfa Ling Jisong Zheng Chen Duan Xumin Lai Mushui Pan Xiaotong Zhu Lina Wang Ping Gao Gang Shu qingyan jiang Songbo Wang Guangdong Provincial Key Laboratory of Animal Nutrition Control College of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China National Engineering Research Center for Breeding Swine Industry and ALLTECH-SCAU Animal Nutrition Control Research Alliance South China Agricultural UniversityGuangzhou510642China Sericultural&Agri-Food Research Institute Guangdong Academy of Agricultural SciencesKey Laboratory of Functional FoodsMinistry of AgricultureGuangdong Key Laboratory of Agricultural Products ProcessingGuangzhou510610China

Nutritional diarrhea and subsequent performance degradation in weaned piglets are major challenges for the pig *** acids(BA)can be added to the diet as *** experiment was conducted to investigate the effects of chenodeoxycholic acid(CDCA),a major primary BA,on growth performance,serum metabolic profiles and gut health in weaned piglets.A total of 72 healthy weaned piglets were randomly assigned to the control(CON)and the CDCA groups,which were feed a basal diet and the basal diet supplemented with 200 mg/kg CDCA for 30 d,*** results demonstrated that CDCA significantly increased final BW and average daily gain(ADG),decreased feed-to-gain(F:G)ratio and tended to reduce diarrhea *** addition,CDCA increased the villus height-to-crypt depth(V:C)ratio,elevated goblet cell numbers and the expression of tight junction proteins,suggesting the enhancement of intestinal barrier *** an emulsifier,CDCA increased jejunal lipase activity and the mRNA expression of pancreatic *** supplementation also altered the serum metabolic profiles,including increasing the levels of indole 3-acetic acid,N'-formylkynurenine and theobromine that were beneficial for gut ***,the relative abundance of 2 beneficial gut bacteria,Pre-votella 9 and Prevotellaceae TCG-001,were increased,whereas the relative abundance of a harmful bacteria,Dorea,was decreased in the gut of weaned piglets supplemented with ***,the altered serum metabolic profiles showed a strong correlation with the changed gut *** conclusion,CDCA improved the growth performance of weaned piglets by improving intestinal morphology and barrier function,and enhancing lipid digestion,accompanied by alterations of serum metabolic profiles,and changes in relative abundance of certain gut bacteria.

关键词： Chenodeoxycholic acid Weaned piglet Growth performance Gut health Serum metabolite Gut microbiota

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中国对美OFDI对母国的就业效应:创造还是替代

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系统工程理论与实践 2022年第9期42卷 2277-2289页

作者：姜青言杨翠红田开兰中国科学院数学与系统科学研究院国家数学与交叉科学中心北京100190 中国科学院大学北京100049

本文从对外直接投资(简称OFDI)动机出发梳理了OFDI对就业的主要影响路径,提出了一种基于时变状态空间模型和投入产出模型的OFDI就业效应测算模型,利用2007-2014年中国对美国制造业OFDI和出口数据以及世界投入产出表,测算了中国对美投资... 详细信息

本文从对外直接投资(简称OFDI)动机出发梳理了OFDI对就业的主要影响路径,提出了一种基于时变状态空间模型和投入产出模型的OFDI就业效应测算模型,利用2007-2014年中国对美国制造业OFDI和出口数据以及世界投入产出表,测算了中国对美投资的母国就业效应和机会成本.研究发现:1)中国对美国OFDI对就业人数的影响总体为创造效应,2008-2014年中国对美国制造业OFDI平均每年为中国创造就业147万人;2)投资动机层面,市场寻求型OFDI对母国就业为创造效应,资源寻求型OFDI对母国就业为替代效应;3)2012年之前中国对美国OFDI对母国就业产生的机会成本较大,资金投往国外造成了母国就业损失,但2012年后这种机会成本逐渐减小,相比国内投资,OFDI对母国就业的创造效应更强.

关键词：对外直接投资母国就业效应投入产出模型状态空间模型

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Accumulation of α-ketoglutaric acid prevents diet-induced obesity by adrenal activation of adipose tissue thermogenesis and lipolysis

Accumulation of α-ketoglutaric acid prevents diet-induced o...

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第三届中国生物物理学会代谢生物学分会学术研讨会

作者：袁业现 Tao Wang Cha Zhang qingyan jiang Gang Shu South China Agricultural University

Exercise is an effective way to prevent and combat obesity,but the underlying chemical and physiological mechanisms are not fully *** we performed a comprehensive serum metabolomics approach comparing metabolites between non-exercise and acute exercise mouse *** identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of exercise in *** showed that substantial accumulation of the tricarboxylic acid cycle intermediate α-ketoglutaric acid（AKG） is a metabolic signature of exercise ***,human plasma AKG level is also negatively correlated with *** elevation of circulating AKG induces brown adipose tissue（BAT） thermogenesis and white adipose tissue（WAT） lipolysis in vivo, and protects against diet-induced *** further found that AKG stimulates the adrenal release of adrenaline through G protein receptor 99（GPR99） expressed in adrenal ***,by using both loss-of-function and gain-of-function mouse models,we showed that GPR99 is essential for AKG-mediated exercise-induced beneficial metabolic *** findings reveal an unappreciated mechanism for the salutary effects of exercise,using AKG as a systemically-derived molecule for adrenal stimulation of adipose tissue thermogenesis and lipolysis.

关键词： AKG GPR99 obesity thermogenesis lipolysis

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Smooth muscle AKG/OXGR1 signaling regulates epididymal fluid acid–base balance and sperm maturation

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Life Metabolism 2022年第1期1卷 67-80页

作者： Chang Xu Yexian Yuan Cha Zhang Yuchuan Zhou Jinping Yang Huadong Yi Ishwari Gyawali Jingyi Lu Sile Guo Yunru Ji Chengquan Tan Songbo Wang Yongliang Zhang qingyan jiang Gang Shu Guangdong Laboratory for Lingnan Modern Agriculture National Engineering Research Center for Breeding Swine Industry and Guangdong Province Key Laboratory of Animal Nutritional RegulationCollege of Animal ScienceSouth China Agricultural UniversityGuangzhouGuangdongChina International Peace Maternity and Child Health Hospital Shanghai Key Laboratory of Embryo Original DiseasesSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory for Aquatic Economic AnimalsSchool of Life SciencesSun Yat-Sen UniversityGuangzhouChina

Infertility is a global concern attributed to genetic defects,lifestyle,nutrition,and any other factors that affect the local metabolism and niche microenvironment of the reproductive system.2-Oxoglutarate receptor 1(OXGR1)is abundantly expressed in the testis;however,its cellular distribution and biological function of OXGR1 in the male reproductive system remain *** the current study,we demonstrated that OXGR1 is primarily expressed in epididymal smooth muscle cells(SMCs).Aging and heat stress significantly reduced OXGR1 expression in the *** OXGR1 global knockout and epididymal-specific OXGR1 knockdown models,we revealed that OXGR1 is essential for epididymal sperm maturation and fluid acid–base *** ofα-ketoglutaric acid(AKG),the endogenous ligand of OXGR1,effectively reversed epididymal sperm maturation disorders caused by aging and heat ***,in vitro studies showed that AKG markedly stimulated the release of instantaneous intracellular calcium from epididymal SMCs and substantially reduced the pH_(i) value in the epididymal SMCs via ***,we discovered that AKG/OXGR1 considerably increased the expression of Na^(+)/HCO_(3)^(−)cotransporter(NBCe1)mRNA in the epididymal SMCs,mediated by intracellular calcium *** local AKG/OXGR1 system changed the epididymal fluid pH value and HCO_(3)^(−)concentration,thereby regulating sperm maturation via intracellular calcium signaling and NBCe1 mRNA *** study for the first time reveals the crucial role of OXGR1 in male fertility and sheds light on the applicability of metabolic intermediates in the nutritional intervention of reproduction.

关键词： AKG OXGR1 epididymis acid-base balance sperm maturation

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