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Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance, Cell Immunity and Serum Biochemical Indices of Weaned Piglets (Sus scrofa)

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Agricultural Sciences in China 2011年第6期10卷 938-945页

作者： WANG Xiu-qi FENG You SHU Gang jiang qing-yan yanG Jing-pei ZHANG Zi-feng College of Animal Science South China Agricultural University Guangzhou 510642 P.R. China Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding Guangzhou 510642 P.R.China Zhengzhou Newwill Nutrition Technology Co. Ltd. Zhengzhou 450000 P.R. China

To investigate the effects of dietary supplementation with hydrolyzed wheat gluten （HWG） on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed （Large White×andrace） and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG （control group）, 30 g kg-1 HWG （3% HWG group）, 50 g kg-1 HWG （5% HWG group）, or 2.5 g kg-1 glycyl-L-glutamine （0.25% Gly-Gln group）. The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 （P〈0.05）, while the average daily gain （ADG） in each of two groups was increased （P〉0.05）. When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4＋：CD8＋ and the ratio of serum albumin and globulin concentrations （A：G） on d 14 and 28, as well as the proliferation of T- and B-lymphocytes （P〉0.05） on d 28. In addition, on d 14 and 28, the A：G ratio in 5% HWG group was significantly higher than in control group （P〈0.05）, while the ratio of CD4＋：CD8＋ increased slightly （P〉0.05）. Interestingly, 0.25% Gly-Gln group had higher proportion of CD3＋（P〉0.05） and CD4＋（P〈0.05） on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.

关键词： hydrolyzed wheat gluten weaned piglets growth performance cell immunity serum biochemical indices

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Prokaryotic Expression of Gly-Gln Dipeptide and Its Bioactive Analysis: A Novel Method for Short Peptide Production

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Agricultural Sciences in China 2010年第5期9卷 736-744页

作者： XU Ping-wen FANG Xin-ling SHU Gang ZHU Xiao-tong LUO Zeng-fu jiang qing-yan GAO Ping ZHANG Yong-liang College of Animal Science South China Agricultural University Guangzhou 510642 P.R.China

Small bioactive peptides, with diverse biological functions, have received increasing attention as physiologically beneficial substances in animal production. The main obstacle to wide application of small bioactive peptides is a lack of costeffective methods for mass production. In this study, we mass-production method for small bioactive peptides. used glycyl-glutamine （Gly-Gln） as a test case to develop a novel The oligonucleotide encoding Gly-Gln pro-peptide （Gpp） was designed and synthesized. Gpp includes 3 Gly-Gln dipeptides and 2 enzymatic sites for pepsin and trypase, allowing direct digestion and absorption of Gly-Gln in the gastrointestinal tract. The Gpp oligonucleotides were linked to generate an oligomeric oligonucleotide segment containing 12 tandem copies of Gpp. This 12Gpp segment was cloned and expressed in Escherichia coli vector pET32a. By optimizing culture conditions [0.1 mmol L^-1 isopropyl-β-D- thiogalactopyranoside （IPTG）, 50 μg mL^-1 ampicillin （Amp）, 30℃ for 12 h], the thioredoxin fusion peptides reached 40% of total bacterial protein. After purification, the fusion protein was fed to Kunming mice to determine its effect on mouse immune function. The results showed that similar to Gly-Gln dipeptide, Gpp polymer protein could significantly suppress the proliferation of T and B lymphocytes in blood and spleen, and additionally could significantly improve interleukin-2 （IL-2） and interleukin-6 （IL-6） secretion of blood and spleen lymphocytes. These effects were not observed in mice fed a 2 amino acids mix （glycine and glutamine）. These evidences indicated that an efficient digestion of Gpp polymer protein could be achieved when ingested into the animal gut. The expression system in this study provides a potential production method for not only Gly-Gln dipeptide but also other short bioactive peptides.

关键词： glycyl-glutamine tandem expression gastrointestinal tract

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Characterization and Differentiation into Adipocytes and Myocytes of Porcine Bone Marrow Mesenchymal Stem Cells

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Journal of Integrative Agriculture 2014年第4期13卷 837-848页

作者： DU Min-qing HUANG Yue-qin LU Nai-Sheng SHU Gang ZHU Xiao-tong WANG Li-na GAO Ping XI Qian-yun ZHANG Yong-liang WANG Song-bo jiang qing-yan College of Animal Sciences South China Agricultural University

Bone marrow mesenchymal stem cells （BMSCs） could differentiate into various cell types including adipocytes and myocytes, which had important scientific significance not only in the field of tissue regeneration, but also in the field of agricultural science. In an attempt to exhibit the characterization and differentiation into adipocytes and myocytes of porcine BMSCs, we isolated and purified porcine BMSCs by red blood cell lysis method and percoll gradient centrifugation. The purified cells presented a stretched fibroblast-like phenotype when adhered to the culture plate. The results of flow cytometry analysis and immunofluorescence staining demonstrated that the isolated cells were positive for mesenchymal surface markers CD29, CD44 and negative for hematopoietic markers CD45 and the adhesion molecules CD31. Cells were induced to differentiate into adipocytes with adipogenic medium containing insulin, dexamethasone, oleate and octanoate. Oil Red O staining demonstrated that the porcine BMSCs successfully differentiated to adipocytes. Moreover, the findings of real-time PCR and Western blotting indicated that the induced cells expressed adipogenic marker genes （PPAR-y, C/EBP-c~, perilipin, aP2） mRNA or proteins （PPAR-3,, perilipin, aP2）. On the other hand, porcine BMSCs were induced into myoctyes with myogenic medium supplemented with 5-azacytidine, basic fibroblast growth factor, chick embryo extract and horse serum. Morphological observation by hochest 33342 staining showed that the induced cells presented as multi-nucleus muscular tube structure. And myogenic marker genes （Myf5, desmin） mRNA or proteins （MyfS, MyoD, myogenin, desmin） were found in the induced cells. In addition, the results of immunofluorescence staining revealed that myogenic marker （Myf5, MyoD, myogenin, desmin, S-MyHC） proteins was positive in the induced cells. Above all, these results suggested that the isolated porcine BMSCs were not only consistent with the characterization of mesenchyma

关键词： porcine bone marrow mesenchymal stem cell adipocyte myocyte

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Anticancer Effects of Zaoxiu Compound Decoction on H_22 Mice with Heps

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Chinese Herbal Medicines 2013年第2期5卷 125-128页

作者： jiang yan-qing ZHANG Wen-yun yan Sheng-ying Tianmu College of Zhejiang A & F University Lin'an People's Hospital

Objective To investigate the anticancer effects of the Zaoxiu Compound Decoction(ZCD) on H22 mice with Heps and the influence on the construction of H22 cells. Methods With in vivo techniques, the antineoplastic drugs were given to hepatoma H22 model mice which were randomly divided into negative control(physiological saline), positive control(cyclophosphamide), high- and low-dose ZCD groups. After 10-d administration, the pathological examinations of tumor tissue, livers, and kidneys in mice were carried out and the inhibitory rate on tumor was calculated. Results The high-and low-dose ZCD had obvious inhibition on H22 cells. The pathological observation showed that high- and low-dose ZCD had a strong inhibitory effect on H22 cells as well as positive control, with few toxic effects to the livers and kidneys in mice. Conclusion ZCD has the anticancer effect and has no obvious toxic effects on the model mice.

关键词： anticancer H22mouse Heps pathological observation Zaoxiu Compound Decoction

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Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system

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Journal of Animal Science and Biotechnology 2013年第1期4卷 65-74页

作者： Zhai Ya-Feng Shu Gang Zhu Xiao-Tong Zhang Zhi-Qi Lin Xia-Jing Wang Song-Bo Wang Li-Na Zhang Yong-Liang jiang qing-yan College of Animal Science South China Agricultural University

Background： o-galactosidase has been widely used in animal husbandry to reduce anti-nutritional factors （such as o-galactoside） in feed. Intestine-specific and substrate inducible expression of a-galactosidase would be highly beneficial for transgenic animal production. Methods： To achieve the intestine-specific and substrate inducible expression of o-galactosidase, we first identified intestine-specific promoters by comparing the transcriptional activity and tissue specificity of four intestine-specific promoters from human intestinal fatty acid binding protein, rat intestinal fatty acid binding protein, human mucin-2 and human lysozyme. We made two chimeric constructs combining the promoter and enhancer of human mucin-2, rat intestinal trefoil factor and human sucrase-isomaltase. Then a modified lac operon system was constructed to investigate the induction of o-galactosidase expression and enzyme activity by isopropyl p-D-]-thiogalactopyranoside （IPTG） and an a-galactosidase substrate, a-lactose. We declared that the research carried out on human （Zhai Yafeng） was in compliance with the Helsinki Declaration and experimental research on animals also followed internationally recognized guidelines. Results： The activity of the human mucin-2 promoter was about 2 to 3 times higher than that of other intestine-specific promoters. In the/ac operon system, the repressor significantly decreased （P 〈 0.05） luciferase activity by approximately 6.5-fold and reduced the percentage of cells expressing green fluorescent protein （GFP） by approximately 2-fold. In addition, the expression level of o-galactosidase mRNA was decreased by 6-fold and a-galactosidase activity was reduced by 8-fold. in line with our expectations, IPTG and a-lactose supplementation reversed （P 〈 O.O5） the inhibition and produced a 5-fold increase of luciferase activity, an 11-fold enhancement in the percentage of cells with GFP expression and an increase in o-galactosidase mfiNA abundance （by about 5-fold

关键词： a-galactosidase Inducible expression Intestine-specific promoters Lac operon

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Boundary Value Problems for Singular Second-Order Functional Differential Equations

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Acta Mathematicae Applicatae Sinica 2002年第2期18卷 249-254页

作者： Hui-zhao Liu, Da-qing jiang, yan WangDepartment of Applied Mathematics, Hebei University of Technology, Tianjin 300130, ChinaDepartment of Mathematics, Northeast Normal University, Changchun 130024, China Department of Applied Mathematics Hebei University of Technology Tianjin China Department of Mathematics Northeast Normal University Changchun China

Positive solutions to the boundary value problem,are obtained by applying the Schauder fixed point theorem, where w(x) is a continuous function denned on [0,1] and f(x,y) is a function defined on (0,1)×(0,∞), which satisfies certain restrictions and may have singularity at y=0. The result corrects and improves an existence theorem due to Erbe and Kong.

关键词： Singular boundary value problem existence Schauder fixed point theorem

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Effects of Central Administration of Glutamine and Alanine on Feed Intake and Hypothalamic Expression of Orexigenic and Anorexigenic Neuropetides in Broiler Chicks

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Journal of Integrative Agriculture 2012年第7期11卷 1173-1180页

作者： Khondowe Paul WANG Song-bo CHEN Sheng-feng YU Jian-jian ZHU Xiao-tong WANG Li-na GAO Ping XI Qian-yun ZHANG Yong-liang SHU Gang jiang qing-yan College of Animal Sciences South China Agricultural University Guangzhou 510642 P.R.China School of Natural Sciences Department of Biological Sciences University of Zambia PO. Box 32379 Lusaka Zambia

Different amino acids have been shown to affect feed intake when injected directly into the central nervous system of birds. In the present study, we investigated the effects of L-glutamine and L-alanine on feed intake and the mRNA expression levels of hypothalamic neuropeptides involved in feed intake regulation in broiler chicks. L-Glutamine or L- alanine was intra-cerebroventricularly （ICV） administered to 4-d-old broiler chicks and the feed intake were recorded at various time points. Quantitative PCR was performed to determine the hypothalamic mRNA expression levels of neuropeptide Y （NPY）, agouti related protein （AgRP）, pro-opiomelanocortin （POMC）, melanocortin receptor 4 （MC4R） and corticotropin releasing factor （CRF）. Our results showed that ICV administration of L-glutamine （0.55 or 5.5 pmol） significantly increased feed intake up to 2 h post-administration period and the hypothalamic NPY mRNA expression levels, while it markedly decreased hypothalamic POMC and CRF mRNA expression levels. In contrast, ICV administration of L-alanine （4 lamol） significantly decreased feed intake for the first 0.5 h post-administration period, and reduced the hypothalamic AgRP mRNA expression levels, while it remarkablely enhanced the mRNA expression levels of MC4R and CRF. These findings suggested that L-glutamine and L-alanine could act within the hypothalamus to influence feed intake in broiler chicks, and that both orexigenic and anorexigenic neuropeptide genes might contribute directly to these effects.

关键词： L-glutamine L-alanine intracerebroventricular (ICV) feed intake hypothalamus

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Intelligent PID guidance control for AUV path tracking

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Journal of Central South University 2015年第9期22卷 3440-3449页

作者：李晔姜言清王磊峰曹建张国成 National Key Laboratory of Underwater Vehicle Harbin Engineering University

Based on rational behavior model of three layers, a tracking control system is designed for straight line tracking which is commonly used in underwater survey missions. An intelligent PID control law implemented as planning level during the control system using transverse deviation is came up with. Continuous tracking of path expressed by a point sequence can be realized by the law. Firstly, a path tracking control system based on rational behavior model of three layers is designed, mainly satisfying the needs of underactuated AUV. Since there is no need to perform spatially coupled maneuvers, the 3D path tracking control is decoupled into planar 2D path tracking and depth or height tracking separately. Secondly, planar path tracking controller is introduced. For the reason that more attention is paid to comparing with vertical position control, transverse deviation in analytical form is derived. According to the Lyapunov direct theory, control law is designed using discrete PID algorithm whose parameters obey adaptive fuzzy adjustment. Reference heading angle is given as an output of the guidance controller conducted by lateral deviation together with its derivative. For the purpose of improving control quality and facilitating parameter modifying, data normalize modules based on Sigmoid function are applied to input-output data manipulation. Lastly, a sequence of experiments was carried out successfully, including tests in Longfeng lake and at the Yellow sea. In most challenging sea conditions, tracking errors of straight line are below 2 m in general. The results show that AUV is able to compensate the disturbance brought by sea current. The provided test results demonstrate that the designed guidance controller guarantees stably and accurately straight route tracking. Besides, the proposed control system is accessible for continuous comb-shaped path tracking in region searching.

关键词： guidance control region search underactuated AUV intelligent PID path tracking

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Myristic Acid(MA) Promotes Adipogenic Gene Expression and the Differentiation of Porcine Intramuscular Adipocyte Precursor Cells

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Journal of Integrative Agriculture 2014年第11期13卷 2488-2499页

作者： LU Nai-sheng SHU Gang XIE Qiu-ping ZHU Xiao-tong GAO Ping ZHOU Gui-xuan WANG Song-bo WANG Li-na XI Qian-yun ZHANG Yong-liang jiang qing-yan College of Animal Sciences South China Agricultural University

Intramuscular fat （IMF） content is considered to be a key factor that affects the marbling, tenderness, juiciness and lfavor of pork. To investigate the effects of myristic acid （MA） on the differentiation of porcine intramuscular adipocytes, cells were isolated from longissimus dorsi muscle （LDM） and treated with 0, 10, 50 or 100μmol L-1 MA. The results showed that MA signiifcantly promotes the differentiation of intramuscular adipocytes in a dose-dependent manner. MA also led to a parallel increase in the expression of peroxisome proliferator activated receptor-γ（PPARγ） and adipose-related genes, such as glucose transporter 1 （GLUT1）, lipoprotein lipase （LPL）, adipocyte fatty acid binding protein 4 （FABP4/aP2）, fatty acid translocase （FAT）, acetyl-CoA carboxylaseα（ACCα）, adipose triglyceride lipase （ATGL） and fatty acid synthase （FASN）. However, no signiifcant effects of MA were observed on the expression of CAAT enhancer binding protein-α（C/EBPα） or hormone sensitive lipase （HSL）. The expression of pyruvate dehydrogenase kinase 4 （PDK4） was increased by MA during the early stages of differentiation （day 1-3）. In addition, MA also increased the absolute content of C14 （P〈0.001） and saturated fatty acids （SFA）（P〈0.05） to varying degrees, but no effects were observed on other fatty acids. These results suggest that MA might be able to enhance the IMF content of pork and increase the accumulation of myristic and myristoleic acid in muscle, which might have beneifcial implications for human health.

关键词： porcine intramuscular fat myristic acid adipogenesis fatty acid composition

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浅议水电工程投标报价策略及编制技巧

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人民长江 2009年第10期40卷 77-78页

作者：江青艳武警水电第一总队项目处广西南宁530028

在工程招标投标活动中,施工企业要想在投标中立于不败之地,中标拿到工程,主要应该在先进合理的技术方案和较低的投标价格上下功夫。要想在工程建设中获得预想的利润,就需要在投标阶段综合考虑各种主观条件与客观因素。在投标阶段,只有... 详细信息

在工程招标投标活动中,施工企业要想在投标中立于不败之地,中标拿到工程,主要应该在先进合理的技术方案和较低的投标价格上下功夫。要想在工程建设中获得预想的利润,就需要在投标阶段综合考虑各种主观条件与客观因素。在投标阶段,只有深入细致地研究投标策略,确定最适宜的投标方法并正确运用编制技巧来达到中标的目的,才能给企业带来好的经济效益。简要地介绍了水电工程投标报价策略及编制技巧。

关键词：投标报价策略报价编制水电工程

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