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Agricultural Sciences in China 2007年第2期6卷 167-174页

作者： BAI Yao-yu jiang ming-xing CHENG Jia-an Institute of Insect Sciences Zhejiang University Hangzhou 310029 P.R.China Chongqing Key Laboratory of Entomology ＆ Insect Control Engineering~College of Plant Protection Southwest University Chongqing 400716 P.R.China

The determination of the environmental fate of Bt insecticidal protein released by Bt rice plants in paddy soils is a key issue in its ecological risk assessment. In this study, the impacts of soil water content, pH, and temperature on the degradation of CrylAb protein expressed in the leaves of Bt rice KMD2 were studied in the laboratory. Three types of paddy soils were used, i.e., blue clayey paddy soil, pale paddy soil on quaternary red soil, and marine-fluvigenic yellow loamy paddy soil. Ground powders of KMD2 leaf blades were mixed with each type of soil, and degradation dynamics of Cry lAb were measured using enzyme-linked immunosorbent assay （ELISA）. The degradation rate of CrylAb was high at the early experimental stage, but slowed down steadily at middle and later stages, which could be described by exponential equations, with the half-life period of degradation determined as 1.8-4.0 d. The soil water content, pH, and temperature could affect the degradation of CrylAb, but the effects of soil pH and temperature were relatively greater. In general, CrylAb degradations were slower under lower soil pH and temperature conditions, especially for marine-fluvigenic yellow loamy paddy soil.

关键词： transgenic Bt rice CrylAb insecticidal protein paddy soil degradation environmental factors

Invasion biology of the cotton mealybug, Phenacoccus solenopsis Tinsley: Current knowledge and future directions

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Journal of Integrative Agriculture 2019年第4期18卷 758-770页

作者： TONG Hao-jie AO Yan LI Zi-hao WANG Ying jiang ming-xing Institute of Insect Sciences Zhejiang University

The cotton mealybug Phenacoccus solenopsis Tinsley(Hemiptera: Pseudococcidae), which is native to North America, emerged as a major invasive insect pest of multiple crops in Asia at the beginning of the 21 st century. Considering the economic significance of this insect and its rapid worldwide spread, we examined the possible factors driving its invasions. In this paper, we summarize the life history traits of P. solenopsis conceivably related to population development in invaded regions; these traits include its use of diverse host plants, reproductive capacity and mode, adaptation to temperature, response to food shortage, and insecticidal resistance. Then, focusing on the multiple trophic interactions that may promote or hinder invasion, we review the mutualistic relationship of this mealybug with ants and predation and parasitism by natural enemies. Finally, we suggest topics for future research and provide our perspective on the biological invasions of this mealybug. We speculate that specific biological factors associated with this mealybug, particularly its wide host range, high reproductive potential, evolved changes in life history traits, and mutualism with ants have played important roles in its invasions, allowing this pest to become established and rapidly increase its population upon its introduction into new regions.

关键词： cotton mealybug biological invasion reproduction mutualism natural enemies adaptation

Physical and Electrochemical Characterization of Hydrothermal Prepared α'-NaV_2O_5 Crystals

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Chemical Research in Chinese Universities 2010年第2期26卷 291-293页

作者： HU Fang WEI Ying-jin jiang Tao ming xing CHEN Gang WANG Chun-zhong College of Physics State Key Laboratory of Superhard Materials Jilin University Changchun 130012 P. R. China

a＇-NaV2O5 was prepared by a simple hydrothermal process. X-ray diffraction confirmed the orthorhombic structure of a＇-NaV2O5, with preferential growth along the （001） direction. Scanning electron microscopy showed a＇-NaV205 was composed of flake-shaped crystals. X-ray photoelectron spectroscopy confirmed the co-existence of V^4＋ and V^5＋ in a＇-NaV2O5, which results in an average V^4.5＋ oxidation state of a＇-NaV2O5. The observed Raman bands are ascribed to different V-O vibrations, a＇-NaV205 shows a reversible specific capacity of about 100 mA·h·g^-1 between 3.5 and 1.0 V, with a good capacity retention. The good electrochemical stability of the material is attributed to its structural stability during Li^＋ intercalation.

关键词： Lithium ion battery Vanadium bronze Electrochemical performance

Oogenesis in summer females of the rice water weevil,Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae),in southern Zhejiang,China

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第1期8卷 33-38页

作者： SHI Sheng-wei jiang ming-xing SHANG Han-wu LV Hui-ping CHENG Jia-an Institute of Insect Sciences Zhejiang University Hangzhou 310029 China Institute of Bioengineering Zhejiang Sci-Tech University Hangzhou 310018 China

The rice water weevil, Lissorhoptrus oryzophilus Kuschel, has two generations in southern Zhejiang, China. To determine oogenesis in first-generation females （summer females） and its relations to temperature, females were collected from a rice field in early and mid-July and reared on young rice plants at 28, 31 and 34 ℃ in the laboratory. Percentage of females having oocytes, number of oocytes of different stages （stage-Ⅰ, from early previtellogenesis to middle vitellogenesis; stage-Ⅱ, late vitellogenesis; and matute-oocyte stage）, and length of ovarioles were determined every 10 d of feeding. At each temperature, oogenesis took place in over 40% of females after 20-40 d of feeding, but only 0.0-3.3 stage-Ⅰ, 0.0-0.8 stage-Ⅱ and 0.0-1.1 mature oocytes were observed at each observation date. Temperature had significant effect on number of stage-Ⅰ oocytes but not on number of stage-Ⅱ and mature oocytes in early July females; temperature had no significant effect on number of oocytes of either stage in mid-July females. Conclusively, in southern Zhejiang, summer L. oryzophilus females have great potential to become reproductive on rice, but their oogenesis activity is very low, with the overall procedures little affected by temperature.

关键词： Lissorhoptrus oryzophilus Oogenesis Ovarian development Reproduction

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金相图像的晶界恢复与重建

光学精密工程 2011年第10期19卷 2541-2549页

作者：蒋明星陈国华华南理工大学机械与汽车工程学院广东广州510640 华南农业大学理学院广东广州510642

针对定量金相分析中金相图像的晶界恢复与重建以及数学形态学在图像处理中的特殊作用,从理论上证明了传统膨胀运算对图像灰度连续性的影响及影响程度与结构元素尺寸的关系,并对传统的膨胀运算的定义做了改进,据此提出了多尺度测地膨胀... 详细信息

针对定量金相分析中金相图像的晶界恢复与重建以及数学形态学在图像处理中的特殊作用,从理论上证明了传统膨胀运算对图像灰度连续性的影响及影响程度与结构元素尺寸的关系,并对传统的膨胀运算的定义做了改进,据此提出了多尺度测地膨胀算法并以此恢复和重建金相图像的晶界。首先依据改进的膨胀运算对金相图像进行预处理;然后用多尺度迭代腐蚀和多尺度测地膨胀方法得到晶粒的种子;最后用条件粗化方法对晶粒的种子进行区域生长、产生晶界线。实验结果表明,与传统的重复测地膨胀方法以及基于微分运算的图像分割等方法相比,本方法不仅可快速恢复与重建晶界线,而且获取的晶界线更加清晰准确、连续光滑。

关键词：晶相图晶界恢复晶界重建膨胀灰度连续性多尺度测地膨胀

Efects of benzyltetrahydropalmatine

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中国药理学与毒理学杂志 1996年第1期 72-73页

作者： DAI Shui-Ping, YAO Wei-xing, XIA Guo-jing, jiang ming-xing, NIU Xiao-Wei 1, ZENG Tao 1, KANG Hua-Guang 1 (Department of Pharmacology, Tongji Medical University, Wuhan 430030 1 Institute of Biophysics and Biochemistry, Huazhong Uni

Ｅｆｅｃｔｓｏｆｂｅｎｚｙｌｔｅｔｒａｈｙｄｒｏｐａｌｍａｔｉｎｅｏｎａｃｔｉｏｎｐｏｔｅｎｔｉａｌｓａｎｄｄｅｌａｙｅｄｒｅｃｔｉｆｙｉｎｇｐｏｔａｓｉｕｍｃｕｒｅｎｔｓｉｎｇｕｉｎｅａｐｉｇｖｅｎｔｒｉｃｕｌａｒ... 详细信息

ＥｆｅｃｔｓｏｆｂｅｎｚｙｌｔｅｔｒａｈｙｄｒｏｐａｌｍａｔｉｎｅｏｎａｃｔｉｏｎｐｏｔｅｎｔｉａｌｓａｎｄｄｅｌａｙｅｄｒｅｃｔｉｆｙｉｎｇｐｏｔａｓｉｕｍｃｕｒｅｎｔｓｉｎｇｕｉｎｅａｐｉｇｖｅｎｔｒｉｃｕｌａｒｍｙｏｃｙｔｅｓＤＡＩＳｈｕｉ－Ｐ...

关键词： benzyltetrahydropalmatine currents, potassium patch clamp technique myocardium

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桥头堡战略下的云南经济发展研究

云南师范大学学报（哲学社会科学版） 2011年第6期43卷 114-121页

作者：蒋兴明云南大学发展研究院云南昆明650021

把云南建设成为中国面向西南开放重要桥头堡,是我国对外经济发展的重要战略之一。文章从经济层面上,分析了云南发展城市经济、开放经济、资源经济、绿色经济、民族经济的现状和特点;对于加快云南建设面向西南开放重要桥头堡,提出了聚集... 详细信息

把云南建设成为中国面向西南开放重要桥头堡,是我国对外经济发展的重要战略之一。文章从经济层面上,分析了云南发展城市经济、开放经济、资源经济、绿色经济、民族经济的现状和特点;对于加快云南建设面向西南开放重要桥头堡,提出了聚集发展城市经济、加快发展开放经济、创新发展资源经济、突破发展绿色经济、跨越发展民族经济等5点建议。

关键词：开放重要桥头堡云南经济发展

基于机器学习的金属有机框架吸附水中重金属性能预测

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中国环境科学 2023年第5期43卷 2319-2327页

作者：姜明星王斯坦许端平辽宁工程技术大学环境科学与工程学院辽宁阜新123000

为了能准确预测金属有机框架材料(MOFs)对水中重金属的吸附性能,收集了48篇文献中的MOFs结构特征和成分特性,以及吸附水中重金属的实验参数建立数据集,训练并评价了6种回归模型,包括支持向量回归(SVR)、K-最近邻(KNN)、提升法(AdaBoost... 详细信息

为了能准确预测金属有机框架材料(MOFs)对水中重金属的吸附性能,收集了48篇文献中的MOFs结构特征和成分特性,以及吸附水中重金属的实验参数建立数据集,训练并评价了6种回归模型,包括支持向量回归(SVR)、K-最近邻(KNN)、提升法(AdaBoost)、梯度提升树(GBDT)、随机森林(RF)和袋装法(Bagging).结果表明:基于树的集成学习模型的预测性能表现优异,其中以GBDT算法训练的模型性能最佳;进一步应用该模型,证明了机器学习方法可以准确预测MOFs对水中重金属的吸附性能;排列重要性与部分依赖图(PDP)显示,除了可控的实验参数外,影响吸附量的重要因素是MOFs的孔径、比表面积、孔体积.本研究中的方法不仅能预测MOFs结构和性能的关系,还可以基于有效的实验参数模拟水中重金属的去除,进而为吸附材料的筛选优化提供参考.

关键词：水处理机器学习金属有机框架重金属吸附预测模型

Bt水稻Cry1Ab杀虫蛋白表达的时间动态及其在水稻土中的降解

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生态学报 2005年第7期25卷 1583-1590页

作者：白耀宇蒋明星程家安浙江大学应用昆虫学研究所

采用ELISA技术检测了Bt水稻克螟稻1号(KMD1)和克螟稻2号(KMD2)不同生育期茎和叶片中cry1Ab基因的表达水平,以及植株组织(茎和叶片)室内埋于不同稻田土壤中其Cry1Ab杀虫蛋白的降解动态。结果表明,不论是KMD1还是KMD2,茎和叶片中cry1Ab基... 详细信息

采用ELISA技术检测了Bt水稻克螟稻1号(KMD1)和克螟稻2号(KMD2)不同生育期茎和叶片中cry1Ab基因的表达水平,以及植株组织(茎和叶片)室内埋于不同稻田土壤中其Cry1Ab杀虫蛋白的降解动态。结果表明,不论是KMD1还是KMD2,茎和叶片中cry1Ab基因的表达量为3.7～9.1μg/g鲜重,均以拔节期和孕穗期相对较低,灌浆初期显著升高,黄熟期又明显下降(但仍然维持在较高的表达量)。KMD1茎和叶片中Cry1Ab蛋白在3种水稻土即青紫泥田、黄松田和黄筋泥田中的降解,均以前期(处理后12d内)较快,但中后期明显较慢;处理后42d,这两种组织中的Cry1Ab残留量均稳定在μg级水平,分别仅有0.20～0.85μg/g干重和0.35～1.81μg/g干重。KMD1茎和叶片中Cry1Ab蛋白降解动态与土壤类型密切相关,青紫泥田中降解最快,黄筋泥田中次之,黄松田中最慢。淹水可加快土壤中茎和叶的腐解,从而促进其中Cry1Ab蛋白的降解。KMD2粉碎叶中Cry1Ab蛋白在有菌水稻土中的降解快于无菌土中,表明土壤微生物存在可加快Cry1Ab蛋白的降解。在各种土壤淹水与非淹水、灭菌与非灭菌处理条件下的Cry1Ab蛋白降解过程均可用指数方程进行拟合,算得其消减半衰期为2.2～11.6d不等。讨论了土壤类型、淹水等影响Cry1Ab蛋白降解的可能机制,认为土壤微生物是其中起关键作用的一个因子。

关键词： Bt水稻 Cry1Ab杀虫蛋白表达水稻土降解

苄基四氢巴马汀细胞内用药对豚鼠乳头状肌动作电位和心室肌细胞延迟整流钾电流的作用

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药学学报 2000年第8期35卷 576-579页

作者：李泱姚伟星夏国瑾江明性同济医科大学基础医学院药理教研室湖北武汉430030

目的　研究将苄基四氢巴马汀 (BTHP)导入细胞内对豚鼠乳头状肌动作电位及单个心室肌细胞延迟整流钾电流的影响。方法　利用外加电压脉冲将药物导入乳头状肌细胞内 ,并用标准微电极方法测定动作电位 ;利用浓度差扩散方式使药物进入单个... 详细信息

目的　研究将苄基四氢巴马汀 (BTHP)导入细胞内对豚鼠乳头状肌动作电位及单个心室肌细胞延迟整流钾电流的影响。方法　利用外加电压脉冲将药物导入乳头状肌细胞内 ,并用标准微电极方法测定动作电位 ;利用浓度差扩散方式使药物进入单个心室肌细胞内 ,采用全细胞膜片钳技术记录延迟整流钾电流 (IK)。结果　 10 0 μmol·L-1BTHP使APD2 0 和APD90 分别延长 13 5 %和 2 0 5 %。 30 μmol·L-1BTHP使IK 和IK ,tail分别从 (14 1± 2 2 )pA·pF-1和 (4 0± 0 6 ) pA·pF-1降至 (9 4± 1 3) pA·pF-1和 (2 1± 1 0 ) pA·pF-1,下降率分别为 33 2 %和 35 3%。该药使IK 和IK ,tail的I V曲线幅度降低 ,对曲线形状影响不明显。结论　BTHP入细胞内后可阻滞延迟整流钾电流和延长动作电位时程。

关键词：苄基四氢巴马汀动作电位延迟整流钾电流