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Metastatic suppressor genes inactivated by aberrant methylation in gastric cancer

World Journal of Gastroenterology 2007年第43期13卷 5692-5698页

作者： jian-feng wang Dong-Qiu Dai Department of Surgical Oncology the FirstAffiliated Hospital China Medical University Dalian UniversityAffiliated Xinhua Hospital Shenyang 110001 Liaoning ProvinceChina Department of Surgical Oncology the FirstAffiliated Hospital China Medical University Shenyang 110001Liaoning Province China

AIM: To screen out the differentially methylated DNA sequences between gastric primary tumor and metastatic lymph nodes, test the methylation difference of gene PTPRG between primary gastric tumor and metastatic lymph nodes, and test the regulatory function of 5-aza- 2-deoxycytidine which is an agent with suppression on methylation and the level of methylation in gastric cancer cell line. METHODS: Methylated DNA sequences in genome were enriched with methylated CpG islands amplification (MCA) to undergo representational difference analysis (RDA), with MCA production of metastatic lymph nodes as tester and that of primary tumor as driver. The obtained differentially methylated fragments were cloned and sequenced to acquire the base sequence, which was analyzed with bioinformatics. With methylation-specific PCR (MSP) and RT-PCR, methylation difference of gene PTPRG was detected between primary tumor and metastatic lymph nodes in 36 cases of gastric cancer. Methylation of gene PTPRG and its regulated expression were observed in gastric cancer cell line before and after being treated with methylation-suppressive agent. RESULTS: Nineteen differentially methylated sequences were obtained and located at 5' end, exons, introns and 3' end, in which KL59 was observed to be located at 9p21 as the first exon of gene p16 and KL22 to be located at promoter region of PRPRG . KL22, as the probes, was hybridized with driver, tester and 3-round RDA products respectively with all positive signals except with the driver. Significant difference was observed in both methylation rate of gene PTPRG and PTPRG mRNA expression rate between primary tumor and metastatic lymph nodes. Demethylation of gene PTPRG, with recovered expression of PTPRG mRNA, was observed after gastric cancer cell line being treated with methylation-suppressive agent. CONCLUSION: Difference exists in DNA methylation between primary tumor and metastatic lymph nodes of gastric cancer, with MCA-RDA as one of the good anal

关键词： Gastric cancer Methylated CpG islandsamplification Representational difference analysis DNA methylation gene PTPRG

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DNA barcoding of genus Toxoptera Koch （Hemiptera： Aphididae） Identification and molecular phylogeny inferred from mitochondrial COl sequences

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Insect Science 2009年第6期16卷 475-484页

作者： jian-feng wang Ge-Xia Qiao Institute of Zoology Chinese Academy of Sciences Beijing College of Biological and Environmental Engineering Shenyang University Shenyang China

Identification of aphid species is always difficult due to the shortage of easily distinguishable morphological characters. Aphid genus Toxoptera consists of species with similar morphology and similar to Aphis in most morphological characters except the stridulatory apparatus. DNA barcodes with 1 145 bp sequences of partial mitoehondrial cytochrome-coxidase I （COI） genes were used for accurate identification of Toxoptera. Results indicated mean intraspecific sequence divergences were 1.33%, whereas mean interspecific divergences were greater at 8.29% （0.13% and 7.79% if T. aurantii 3 and T. aurantii 4 are cryptic species）. Sixteen samples were distinguished to four species correctly by COI barcodes, which implied that DNA barcoding was successful in discrimination of aphid species with similar morphology. Phylogenetic relationships among species of this genus were tested based on this portion of COI sequences. Four species of Toxoptera assembled a clade with low support in maximum-parsimony （MP） analysis, maximumlikelihood （ML） analysis and Bayesian phylogenetic trees, the genus Toxoptera was not monophyletic, and there were two sister groups, such as T. citricidus and T. victoriae, and two clades of T. aurantii which probably presented cryptic species in the genus.

关键词： Aphididae barcoding citrus aphid COI molecular phylogeny Toxoptera

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A Berry-Esseen Theorem and a Law of the Iterated Logarithm for Asymptotically Negatively Associated Sequences

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Acta Mathematica Sinica,English Series 2007年第1期23卷 127-136页

作者： jian feng wang Li Xin ZHANG Department of Statistics Zhejiang Gongshang University Hangzhou 310035 P R China Department of Mathematics Zhejiang Unversity Hangzhou 310028 P. R. China

Negatively associated sequences have been studied extensively in recent years, Asymptotically negative association is a generalization of negative association, In this paper a Berry Esseen theorem and a law of the ite... 详细信息

关键词： Berry-Esseen theorem law of the iterated logarithm negative association asymptotically negative association

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Stray light analysis of the Xinglong 2.16-m telescope

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Research in Astronomy and Astrophysics 2020年第3期20卷 50-61页

作者： Tao-Ran Li jian-feng wang Xiao-Ming Zhang Yong Zhao jian-feng Tian Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

An effort towards understanding the problems associated with stray light related to the Xinglong2.16-m telescope is presented to estimate the stray light performance of the telescope itself and provide a method for improving stray light *** light analysis for the 2.16-m telescope model,which consists of an onion shaped dome,telescope structure,equatorial mount and telescope optics,has been developed in two cases(1)pointing to 60?and(2)pointing to zenith,in both azimuth and elevation *** Point Source Normalized Irradiance Transmittance(PSNIT),which is generally used for assessing stray light and is uncorrelated to entrance aperture,is calculated with a series of off-axis *** shows that the PSNIT values are less than 10-7 when off-axis angles are larger than±20?.The dominant contributors to stray light(primary and secondary mirror,telescope structure and dome)are identified to guide performance *** analyses indicate that significant benefit can be realized by adding only five vanes inside the bottom portion of the secondary *** the case of pointing to zenith,the PSNIT values will decrease about 40%on average.

关键词： telescope scattering methods:data analysis

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The Toughening Behavior of the PP/POE Alternating Multilayered Blends under EWF and Impact Tensile Methods

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Chinese Journal of Polymer Science 2015年第10期33卷 1477-1490页

作者： Chun-hai Li jian-feng wang Ji-wei Guo 吴宏郭少云 The State Key Laboratory of Polymer Materials Engineering Polymer Research Institute of Sichuan University

The multilayered polypropylene （PP） and poly（ethylene-co-octene）（POE） sheets were prepared by the microlayered co-extrusion system. The essential work of fracture （EWF） and the impact tensile methods have been successfully used to evaluate the toughening behaviors of the PP/POE multilayered blends under quasi-static and dynamic uniaxial tensile stress, respectively. The experimental results indicate that the multilayered structure plays a key role in the toughening behaviors. On increasing the layer number of the multilayered blends, the specific essential work of fracture, we, increases obviously. As for the flwp, there is no obvious variation in the multilayered blends with low POE content （6.79%）, however, obvious enhancement is observed with increasing the layer number of the high POE content multilayered blends （16.57%）. Compared with the conventional blends, the multilayered blends with 6.79% POE content are effective to increase the value of we. Additionally, the multilayered blends with high layer numbers present absolute advantage in improving the impact tensile values.

关键词： Multilayer EWF Impact tensile PP POE

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The Complete Classification of Graphs whose Second Largest Eigenvalue of the Eccentricity Matrix is Less Than 1

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Acta Mathematica Sinica,English Series 2024年第7期40卷 1741-1766页

作者： jian feng wang Xing Yu LEI Shu Chao LI Zoran STANIC School of Mathematics and Statistics Shandong University of TechnologyZibo255049P.R.China Faculty of Mathematics and Statistics Central China Normal UniversityWuhan430079P.R.China Faculty of Mathematics University of BelgradeStudentski trg 1611000BelgradeSerbia

The eccentricity matrix of a graph is obtained from the distance matrix by keeping the entries that are largest in their row or column,and replacing the remaining entries by *** matrix can be interpreted as an opposite to the adjacency matrix,which is on the contrary obtained from the distance matrix by keeping only the entries equal to *** the paper,we determine graphs having the second largest eigenvalue of eccentricity matrix less than 1.

关键词： Eccentricity matrix eigenvalue diameter principal submatrix forbidden subgraph

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Potential role of the compound Eucommia bone tonic granules in patients with osteoarthritis and osteonecrosis: A retrospective study

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World Journal of Clinical Cases 2020年第1期8卷 46-53页

作者： Cheng-Xia Hu Ke-Yao Hu jian-feng wang Department of Pain Haiyang People’s HospitalYantai 265100Shandong ProvinceChina Department of Urology Yantaishan HospitalYantai 264001Shandong ProvinceChina Department of Pain Qilu Hospital of Shandong UniversityJinan 250012Shandong ProvinceChina

BACKGROUND Osteoarthritis is a major source of pain,disability,and socioeconomic cost *** is a disabling disorder that frequently occurs in the younger population aged from 20-50 *** compound Eucommia bone tonic granules,a traditional Chinese medicine,can alleviate the damage of osteoarthritis and *** To investigate the potential role of the compound Eucommia bone tonic granules(Eucommia)in the treatment of patients with osteoarthritis and *** One-hundred forty osteoarthritis and osteonecrosis cases admitted to our hospital from January 2013 to December 2017 were *** were divided into two groups:Eucommia-meloxicam group and meloxicam *** efficacy and the Western Ontario and McMaster Universities Arthritis Index(WOMAC)score were evaluated according to the evaluation criteria of orthopedic *** levels of bone-GLA protein,interleukin-17,recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin in peripheral blood were *** The total effective rate in the two osteoarthritis groups was not different,but the total effective rate in the two osteonecrosis groups was significantly *** overall efficacy of Eucommia-meloxicam group was superior to that of the meloxicam *** showed that pain,stiffness,and dysfunction in the two groups of osteoarthritis and osteonecrosis before and after treatment were significantly *** concentration of recombinant human S100 calcium binding protein A12,sphingosine 1-phosphate,cystatin C,creatinine,and hemoglobin before and after treatment in the Eucommia-meloxicam group and meloxicam group of osteoarthritis and osteonecrosis were significantly different,and the two treatment groups were significantly different from each other for *** Our findings indicate that Eucommia can effectively enhance the curative effect of meloxicam,and the combinat

关键词： Osteoarthritis Osteonecrosis Eucommia Patients Meloxicam Drug

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Effects of magnesium and copper additions on tensile properties of Al-Si-Cr die casting alloy under as-cast and T5 conditions

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China Foundry 2023年第1期20卷 12-22页

作者： Hong-yi Zhan Yi-wu Xu Pan wang jian-feng wang Jin-ping Li Le-peng Zhang General Motors Global Research&Development China Science LaboratoryShanghai 201206China School of Materials Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China General Motors(China)Investment Co. Ltd.Shanghai 201206China China International Intellectech Corporation(CIIC) Beijing 100004China

Aluminum high pressure die casting(HPDC)technology has evolved in the past decades,enabling stronger and larger one-piece casting with significant part *** also offers a higher design freedom for more mass-efficient thin-walled body *** body structures that require excellent ductility and fracture toughness to be joined with steel sheet via self-piercing riveting(for instance,shock towers and hinge pillars,etc.),a costly T7 heat treatment comprising a solution heat treatment at elevated temperatures(450℃-500℃)followed by an over-ageing heat treatment is needed to optimize microstructure for meeting product *** enable cost-efficient mass production of HPDC body structures,it is important to eliminate the expensive T7 heat treatment without sacrificing mechanical *** die cast alloy chemistry is a potential solution to improve fracture toughness and ductility of the HPDC *** present study intends to tailor the Mg and Cu additions for a new Al-Si-Cr type die casting alloy(registered as A379 with The Aluminum Association,USA)to achieve the desired tensile properties without using T7 heat *** was found that Cu addition should be avoided,as it is not effective in enhancing strength while degrades tensile *** addition is very effective in improving strength and has minor impact on tensile *** investigated Al-Si-Cr alloy with a nominal composition of Al-8.5wt.%Si-0.3wt.%Cr-0.2wt.%Fe shows comparable tensile properties with the T7 treated AlSi10MnMg alloy which is currently used for manufacturing shock towers and hinge pillars.

关键词： Al-Si alloy intermetallics high pressure die casting tensile property T7 heat treatment

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Research on performances of back-illuminated scientific CMOS for astronomical observations

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Research in Astronomy and Astrophysics 2021年第10期21卷 268-278页

作者： Peng Qiu Yong Zhao Jie Zheng jian-feng wang Xiao-Jun jiang CAS Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of ScienceBeijing 100101China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China

To evaluate performances of a back-illuminated scientific CMOS(sCMOS)camera for astronomical observations,comparison tests between Andor Marana s CMOS and Andor iKon-L 936 CCD cameras were conducted in a laboratory and on a *** laboratory tests showed that the readout noise of the sCMOS camera is about half lower,the dark current is about 17 times higher,the dynamic range is lower in the 12-bit setting and higher in the 16-bit setting,and the linearity and bias stability are comparable relative to those of the CCD *** field tests,we observed the open cluster M67 with the sCMOS and CCD cameras on a 60 cm *** the CCD camera,the sCMOS camera has a dual-amplifier *** a 16-bit image of the sCMOS camera is composed of two 12-bit images sampled with 12-bit high gain and low gain amplifiers simultaneously,it is not real 16-bit output *** evaluation tests indicated that the dual-amplifier architecture of the s CMOS camera leads to a decline of photometric stability by about six times around specific pixel *** photometry of bright objects with similar magnitudes that require high frame rates,the s CMOS camera under 12-bit setting is a good ***,the sCMOS camera is fitted with survey observations of variable objects requiring short exposure times,mostly less than 1 s,and high frame *** also satisfies the requirements for an offset guiding instrument owing to its high sensitivity,high temporal resolution and high stability.

关键词： detectors photometric observational

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Charge-controlled compositional/microstructural regulations and electrochemical actuation performance of bulk nanoporous Ni-Pd alloys

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Rare Metals 2024年第10期43卷 5305-5317页

作者： Fu-Quan Tan Qing-Guo Bai Bin Yu jian-feng wang Zhong-Hua Zhang Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials(Ministry of Education) School of Materials Science and EngineeringShandong UniversityJinan 250061China School of Applied Physics and Materials Wuyi UniversityJiangmen 529020China Ira A.Fulton School of Engineering Arizona State UniversityTempeAZ85287USA

Bulk nanoporous(np)metallic actuators have attracted increasing attention due to their large strain and low stimulation ***,studies focusing upon the combined effect of composition and structure on the actuation performance of metallic actuators are relatively scarce,and its underlying mechanism needs to be clarified Herein,a series of bulk np-NiPd samples with differen compositions and microstructures were fabricated using a dealloying-coarsening-dealloying strategy and chargecontrolled electrochemical dealloying,and the process involves only one component of precursor *** has been found that the np-NiPd cubes show a composition/structure-dependent mechanical property and electrochemical actuation ***,the npNi_(70)Pd_(30)sample with a homogeneously porous structure and good network connectivity exhibits significantly larger strain amplitude and faster strain rate than other hierarchically porous NiPd samples(np-Ni_(50)Pd_(50)and npNi_(20)Pd_(80)).Moreover,the np-Ni_(70)Pd_(30)sample demonstrates good actuation stability with high strain retention after hundreds of ***,the maximum strain amplitude(1.17%)is even comparable to that of advanced leadfree piezoceramic,and the maximum strain rate exceeds those of many reported metallic actuator *** work indicates that good network connectivity plays a vita role in facilitating large/fast strain response in metallic actuators.

关键词： Metallic actuators Bulk nanoporous NiPd Electrochemical dealloying Actuation stability Good network connectivity

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