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2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者：孙羽灵 YANG Yan QIAN Lin-nan GUAN Dong-lang jian tun-yu TANG Zong-xiang School of Medicine ·Holistic Integrative Medicine Nanjing University of Chinese Medicine

Backgrounds:Raw lacquer is an important chemical raw material needed in production and life,but the application and promotion of lacquer was limited by its high *** main component of lacquer is urushiol, and it also has strong sensitization as raw *** who are allergic to urushiol may develop a wart-like or wheal-like rash, with symptoms of burning, swelling,and ***, the mechanism of the intense itching induced by urushiol and the corresponding clinical treatment is still not *** hope to explore the mechanism and search therapeutic ***: 1) To explore the mechanism of urushiol-induced allergic pruritus related with mast cells.2) To explore how aloe-emodin can reduce urushiol-induced itching by inhibiting degranulation of mast cells.3) To search the pruritus substances and the signaling pathway released during the degranulation of mast ***:1) We extracted urushiol from fresh raw lacquer using suspension drop method.2)Scratching numbers of mice were used to evaluate the degree of pruritus induced by urushiol and reduceded by aloe-emodin in behavior tests.3) The difference in the number of mast cells and the number of degranulation of mast cells in skin sections was determined by hematoxylin-eosin staining and toluidine blue staining.4) qRT-PCR, Western Blot, ELISA and *** used to determine the pruritus substance which released by the degranulation of mast cells;5) We cultured DRG neurons from wildtype mice to find receptors and signaling pathways of pruritus substances using calcium imaging ***:1) 2% urushiol can cause acute scratching *** with the acetone control group, the scratching numbers was significantly increased during the first hour after 2% urushiol was applied to the neck of the mouse;Four hours later, the scratching numbers of the mouse decreased significantly compared with the first hour, but there was still a significant difference compared with the control g

关键词： urushiol mast cell degranulation aloe-emodin itch

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山药中的致痒物质及致痒机制研究（英文）

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生物化学与生物物理进展 2019年第10期46卷 1012-1019页

作者：杨雁孙羽灵刘培钱林楠关东朗简暾昱朱婵于光王长明朱静李德峰唐宗湘南京中医药大学医学与生命科学学院精神疾病与中医药防治重点实验室南京210023 南京中医药大学药学院南京210023 江苏省植物研究所南京210014 )中国科学院微生物研究所北京100101

山药是一种传统中药,对人体有许多益处,如抗腹泻、抗炎、抗糖尿病、低胆固醇血症、抗氧化、抗肿瘤和免疫调节.山药黏液接触皮肤,常引起严重瘙痒.而山药中存在的致痒物质成分尚不清楚.我们采用乙醇提取、膜过滤、离子交换色谱、悬浮液滴... 详细信息

山药是一种传统中药,对人体有许多益处,如抗腹泻、抗炎、抗糖尿病、低胆固醇血症、抗氧化、抗肿瘤和免疫调节.山药黏液接触皮肤,常引起严重瘙痒.而山药中存在的致痒物质成分尚不清楚.我们采用乙醇提取、膜过滤、离子交换色谱、悬浮液滴法从新鲜山药中提取尿囊素晶体.从河南焦作山药中提取的尿囊素含量约为3.567 mg/g.活性实验结果表明,尿囊素引起的小鼠抓挠反应次数显著地高于对照组.尿囊素直接激活背根神经节神经元,诱导钙流入,还可以诱导神经元内向电流产生.我们首次在细胞水平证明尿囊素能够激活神经细胞,诱导痒觉信号传导.

关键词：山药尿囊素钙离子内流背根神经节

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菱角壳中多酚的纯化及α-糖苷酶抑制活性研究

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时珍国医国药 2017年第11期28卷 2628-2630页

作者：吕寒简暾昱陈剑马丽任冰如李维林江苏省中国科学院植物研究所江苏省抗糖尿病药物筛选服务中心江苏南京210014

目的优选菱角壳多酚的纯化工艺条件,考察菱角壳多酚的药理活性。方法考察11种不同型号大孔树脂吸附洗脱能力,优化富集纯化菱角壳多酚的工艺参数。对菱角壳多酚提取物进行α-糖苷酶抑制活性试验。结果 LS-300B树脂对菱角壳多酚的吸附性... 详细信息

目的优选菱角壳多酚的纯化工艺条件,考察菱角壳多酚的药理活性。方法考察11种不同型号大孔树脂吸附洗脱能力,优化富集纯化菱角壳多酚的工艺参数。对菱角壳多酚提取物进行α-糖苷酶抑制活性试验。结果 LS-300B树脂对菱角壳多酚的吸附性能最好,最佳工艺为LS-300B树脂每1g上样相当于3.0g原药材干品粉末的提取液,水、50%乙醇,依次洗脱50%乙醇洗脱液浓缩、干燥,得总多酚提取物,纯度为91.7%。用最佳工艺得到的菱角壳多酚提取物α-葡萄糖苷酶抑制活性IC50=(0.0085±0.00031)μg·ml^(-1)。结论 LS-300B树脂吸附是富集纯化菱角壳多酚类成分的理想方法。菱角壳多酚类成分有良好的α-葡萄糖苷酶抑制活性。

关键词：菱角壳多酚大孔吸附树脂 α-葡萄糖苷酶抑制

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Loss-of-function mutations of microRNA-142-3p promote ASH1L expression to induce immune evasion and hepatocellular carcinoma progression

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World Journal of Gastroenterology 2025年第1期31卷 126-145页

作者： Xing-Hui yu Yan Xie jian yu Kun-Ning Zhang Zhou-Bo Guo Di Wang Zhao-Xian Li Wei-Qi Zhang yu-Ying Tan Li Zhang Wen-Tao jiang School of Medicine Nankai UniversityTianjin 300192China Tianjin Key Laboratory of Molecular Diagnosis and Treatment of Liver Cancer Tianjin First Center HospitalTianjin 300192China Department of Liver Transplantation Tianjin First Center HospitalTianjin 300192China First Central Clinical School Tianjin Medical UniversityTianjin 300192China

BACKGROUND Hepatocellular carcinoma(HCC)has been a pervasive malignancy throughout the world with elevated *** therapeutic targets are beneficial to treat and predict the ***,the exact molecular mechanisms leading to the progression of HCC are still *** has shown that the microRNA-142-3p level decreases in HCC,whereas bioinformatics analysis of the cancer genome atlas database shows the ASH1L expression increased among liver tumor *** this paper,we will explore the effects and mechanisms of microRNA-142-3p and ASH1L affect the prognosis of HCC patients and HCC cell bioactivity,and the association between *** To investigate the effects and mechanisms of microRNA-142-3p and ASH1L on the HCC cell bioactivity and prognosis of HCC *** In this study,we grouped HCC patients according to their immunohistochemistry results of ASH1L with pathological tissues,and retrospectively analyzed the prognosis of HCC ***,explored the roles and mechanisms of microRNA-142-3p and ASH1L by cellular and animal experiments,which involved the following experimental methods:Immunohistochemical staining,western blot,quantitative real-time-polymerase chain reaction,flow cytometric analysis,tumor xenografts in nude mice,*** statistical methods involved in this study contained t-test,one-way analysis of variance,theχ^(2)test,the Kaplan-Meier approach and the log-rank *** In this study,we found that HCC patients with high expression of ASH1L possess a more recurrence rate as well as a decreased overall survival ***1L promotes the tumorigenicity of HCC and microRNA-142-3p exhibits reduced expression in HCC tissues and interacts with ASH1L through targeting the ASH1L 3′untranslated ***,microRNA-142-3p promotes apoptosis and inhibits proliferation,invasion,and migration of HCC cell lines in vitro via *** the exploration mechanism,we found ASH1L may promote an immunosuppressive mi

关键词： Hepatocellular carcinoma MicroRNA-142-3p ASH1L Immune evasion Tumor immune microenvironment Apoptosis

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Isolation and Purification of Pruritic Compounds from Yam(Dioscoreae Rhizoma)

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神经药理学报 2019年第4期 16-16页

作者： SUN yu-ling YANG Yan QIAN Lin-nan GUAN Dong-lang jian tun-yu TANG Zong-xiang School of Medicine and Life Sciences Nanjing University of Chinese Medicine

Backgrounds:Chinese yam is a traditional Chinese medicine. It has many benefits for people,such as antidiarrhea,anti-inflammation,antidiabetic,hypocholesterolemia,antioxidation,antitumor,and immunomodulation. The mucus of the fresh yam could induce itch in the skin,however,the pruritus compound in yam is very unclear. Objective:1) To isolate and purify the pruritus compounds from fresh Chinese yam;2) To test the activity of the pruritus compound-allantoin;3) To study the cellular mechanism of allantoin-induced itching. Methods:We extracted allantoin crystal from fresh Chinese yam using suspension drop method. Multidimensional chromatographic separation methods including membrane filtration,ion exchange chromatography,C18 reverse phase chromatography,silica column and preparation liquid phase,mass spectrometry analysis were used to purify the active compounds. Scratch numbers of mice were used to evaluate the degree of pruritus induced by allantoin in behavior tests. We cultured DRG neurons from Pirt-GCaMP3 heterozygotes examined their responses to allantoin using calcium imaging techniques. We also examined the electrophysiological characteristics of the neurons induced by allantoin using whole cell recording. Results:We got the allantoin crystal by simple ethanol extraction. The content of allantoin extracted from yam( origin from Jiaozuo,Henan) is about 3.567 mg·g-1. Our results show that allantoin could induce more scratch numbers in mice than control group. Allantoin also directly activated DRG neurons to induce calcium influx and inward current. Conclusion:This is the first time to obtain allantoin crystal from fresh yam. Allantoin is widely found in many natural plants,but the effect of allantoin on DRG neurons and animal itching behavior was the first time. Our research shows that allantoin not only activated small DRG neurons,but also caused itch in mice. Allantoin could induce the itching behavior of mice,which not only help us understand the cause of yam t

关键词： allantoin calcium influx inward current dorsal horn ganglion itch

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Perilipin-2 mediates ferroptosis in oligodendrocyte progenitor cells and myelin injury after ischemic stroke

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Neural Regeneration Research 2025年第7期20卷 2015-2028页

作者： jian Yang jiang Wu Xueshun Xie Pengfei Xia Jinxin Lu Jiale Liu Lei Bai Xiang Li Zhengquan yu Haiying Li Department of Neurosurgery&Brain and Nerve Research Laboratory The First Affiliated Hospital of Soochow UniversitySuzhouJiangsu ProvinceChina Institute of Stroke Research Soochow UniversitySuzhouJiangsu ProvinceChina

Differentiation of oligodendrocyte progenitor cells into mature myelin-forming oligodendrocytes contributes to *** of remyelination due to oligodendrocyte progenitor cell death can result in severe nerve *** is an iron-dependent form of regulated cell death caused by membrane rupture induced by lipid peroxidation,and plays an important role in the pathological process of ischemic ***,there are few studies on oligodendrocyte progenitor cell *** analyzed transcriptome sequencing data from GEO databases and identified a role of ferroptosis in oligodendrocyte progenitor cell death and myelin injury after cerebral *** analysis suggested that perilipin-2(PLIN2)was involved in oligodendrocyte progenitor cell ***2 is a lipid storage protein and a marker of hypoxia-sensitive lipid droplet *** further investigation,we established a mouse model of cerebral ischemia/*** found significant myelin damage after cerebral ischemia,as well as oligodendrocyte progenitor cell death and increased lipid peroxidation levels around the infarct *** ferroptosis inhibitor,ferrostatin-1,rescued oligodendrocyte progenitor cell death and subsequent myelin *** also found increased PLIN2 levels in the peri-infarct area that co-localized with oligodendrocyte progenitor ***2 knockdown rescued demyelination and improved neurological *** findings suggest that targeting PLIN2 to regulate oligodendrocyte progenitor cell ferroptosis may be a potential therapeutic strategy for rescuing myelin damage after cerebral ischemia.

关键词： bioinformatics bulk RNA sequencing ferroptosis ischemic stroke myelin injury oligodendrocyte progenitor cell perilipin-2 single-cell RNA sequencing

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Equivalence checking between SLM and TLM using coverage directed simulation

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Frontiers of Computer Science 2015年第6期9卷 934-943页

作者： jian HU tun LI Sikun LI School of Computer National University of Defense Technology Changsha 410073 China

The increasing complexity of digital systems makes designers begin to design using abstract system level modeling （SLM）. However, SLM brings new challenges for verification engineers to guarantee the functional equivalence between SLM specifications and lower-level implementa- tions such as those of transaction level modeling （TLM）. This paper proposes a novel method for equivalence checking be- tween SLM and TLM based on coverage directed simulation. Our method randomly simulates an SLM model and uses an satisfiability modulo theories （SMT） solver to generate stimuli for the uncovered area with the direction of a com- posite coverage metric （code coverage and functional cover- age）. Then we run all the generated stimuli （random stimuli and direct stimuli） on both SLM and TLM designs. At the same time, the selected observation variables are compared to evaluate the equivalence between SLM and TLM. Promising experimental results show that our equivalence checking method is more efficient with lower simulation cost.

关键词： system level modeling transaction level model- ing equivalence checking composite coverage SMT

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Application Specified Soft-Error Failure Rate Analysis Using Sequential Equivalence Checking Techniques

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Tsinghua Science and Technology 2020年第1期25卷 103-116页

作者： tun Li Qinhan yu Hai Wan Sikun Li School of Computer Science National University of Defense TechnologyChangsha 410073China the Laboratory of Software Engineering for Complex Systems National University of Defense TechnologyChangsha 410073China. School of Software Tsinghua UniversityBeijing 100084China

Soft errors have become a critical challenge as a result of technology scaling. Existing circuit-hardening techniques are commonly associated with prohibitive overhead of performance, area, and power. However,evaluating the influence of soft errors in Flip-Flops(FFs) on the failure of circuit is a difficult verification ***, we proposed a novel flip-flop soft-error failure rate analysis methodology using a formal method with respect to application behaviors. Approach and optimization techniques to implement the proposed methodology based on the given formula using Sequential Equivalence Checking(SEC) are introduced. The proposed method combines the advantage of formal technique-based approaches in completeness and the advantage of application behaviors in accuracy to differentiate vulnerability of components. As a result, the FFs in a circuit are sorted by their failure rates, and designers can use this information to perform optimal hardening of selected sequential components against soft errors. Experimental results of an implementation of a SpaceWire end node and the largest ISCAS’89 benchmark sequential circuits indicate the feasibility and potential scalability of our approach. A case study on an instruction decoder of a practical 32-bit microprocessor demonstrates the applicability of our method.

关键词： soft error failure rate analysis Sequential Equivalence Checking(SEC) application specified

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DAO媒介:驱动未来社会算法化与自组织的元媒介

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新疆师范大学学报（哲学社会科学版） 2025年第1期46卷 132-141页

作者：喻国明苏健威北京师范大学新闻传播学院北京师范大学传播创新与未来媒体实验平台北京100875

DAO媒介是以区块链算法等技术为基础,支持数字社群协同自治的媒介,具有区块链、智能合约与投票等功能特性和要素确权、技术信任、规则构架以及场景聚合等价值禀赋。对于数字生态显露的各种问题,“算法化”是重构数字生态、降解社会系统... 详细信息

DAO媒介是以区块链算法等技术为基础,支持数字社群协同自治的媒介,具有区块链、智能合约与投票等功能特性和要素确权、技术信任、规则构架以及场景聚合等价值禀赋。对于数字生态显露的各种问题,“算法化”是重构数字生态、降解社会系统复杂性的关键机制,DAO媒介是支撑“自组织式”算法化的元媒介。DAO媒介与数字文明时代喷发的微粒个体、微资源、微价值、微需求、微场景等相匹配,能够深度匹配人的个性与自我,形成有效的趣缘连接,进而极大地释放数字文明的生产力。DAO媒介将深刻影响社会系统的构造,将微粒个体与相关社会要素“包裹”为闭环子系统,支持其准开放的系统边界、有效的异质性整合、可持续的规则重构、子系统与父级系统的持续分化和快速进化,等等。在数字文明时代,DAO媒介将使“重新部落化”的“地球村”变为现实,其驱动构筑的DAO社会将更加多元、更加富有创造力,不断拓展人类群体实践的自由度,使人类在高度异质性聚合基础上更加和谐有序地整合协同。

关键词： DAO 数字媒介算法自组织社会系统

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Influence of Grain Boundary on Melting

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Chinese Physics Letters 2001年第9期18卷 1242-1244页

作者： WANG tun ZHOU Fu-Xin LIU yu-Wu State Key Laboratory of Nonlinear Mechanics Institute of MechanicsChinese Academy of SciencesBeijing 100080

The temperature behaviour of an Al bicrystal with surfaces consisting of (110) and (111) crystals is simulated using molecular dynamics. The result shows that the (110) crystal losses its crystalline order at 820K, whereas the disorder does not propagate through the (111) crystal at this temperature. Instead, some disordered atoms are recrystallized into the (111) crystal and the initial grain boundary changes into a stable order-disorder interface. Thus, it was discovered that at a temperature near its melting point, the (111) crystal grew and obstructed the propagation of disorder. Such an obstruction is helpfulfor understanding melting.

关键词： temperature. dynamics. crystal

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