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Cloning and Expression of One Fibrinolytic Enzyme from Bacillus sp. zlw-2

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Agricultural Sciences in China 2009年第5期8卷 591-596页

作者： HAN Xin-mian GUO Run-fang YU Hong-wei jia ying-min Department of Biology Engineering Agricultural University of Hebei Baoding 071000 P.R.China

The gene encoding fibrinolytic enzyme from Bacillus sp. zlw-2 was cloned and sequenced （accession no. EU734749）, which was 1146 bp, encoded 381 amino acids and had 99% homology with Nattokinase YF308 and NAT. The genes encoding pre-pro-fibrinolytic enzyme （including signal peptide, propeptide, and mature peptide） and fibrinolytic enzyme （including mature peptide） were cloned into pET28a vector respectively and then transformed into Escherichia coli BL21 （DE3）. The recombinant ofpre-pro-fibrinolytic enzyme showed enzyme activity of 183 U mL^-1, while no detectable enzyme activity could be found from the recombinant of the mature peptide.

关键词： fibrinolytic enzyme Nattokinase clone recombinant expression

Medicine in Future and Advantages of Integrated Chinese and Western Medicine

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Chinese Journal of Integrative Medicine 2019年第2期25卷 87-90页

作者： ZHANG jiang-hua WU Mi-shan WANG Yue-fen jia ying-min LI En School of Integrated Traditional Chinese and Western Medicine Hebei Medical University Department of Calorimetry Hebei College of Traditional Chinese Medicine Department of Nephropathy Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University Department of Nephrology Shunyi BranchBeijing Hospital of Traditional Chinese Medicine

The history of medical development shows that oriental medicine, or traditional medicine, was born through medical practice during the times when science and technology were immature and underdeveloped, whereas with the development of science and technology, Western medicine, or modern medicine, was born through experimental analysis and research. With the development of medicine, the pros and cons of both medical systems become increasingly evident. How to integrate them and learn from each other will be the direction of future development of medicine. The formation and development of integrated medicine will, inevitably, usher in a new era for medicine.

关键词： Chinese medicine Western medicine integrated Chinese and Western medicine advantage thinking of model

Improved Robust Adaptive Control of a Fluidized Bed Combustor for Sewage Sludge

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自动化学报 2005年第4期31卷 562-566页

作者： MENG Hong-Xia jia ying-min The Seventh Research Division Beijing University of Aeronautics and AstronauticsBeijing 100083

This paper presents a robust model reference adaptive control scheme to deal with un-certain time delay in the dynamical model of a ?uidized bed combustor for sewage sludge. Thetheoretical analysis and simulation results show that the proposed scheme can guarantee not onlystability and robustness, but also the adaptive decoupling performance of the system.

关键词： Robust adaptive control fluidized bed combustor sewage sludge combustion time delay

An Improved Elastic Net for Cancer Classification and Gene Selection

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自动化学报 2010年第7期36卷 976-981页

作者： LI Jun-Tao jia ying-min The Seventh Research Division Beihang University (BUAA) Beijing 100191 P.R.China

This paper presents an improved elastic net to identify relevant genes for cancer *** introducing the data-driven weight coe-cients, the improved elastic net can adaptively select genes in groups and reduce the shrinkage bias for the coe-cients of significant genes. Moreover, the irrelevant observations on the augmented dataset are removed and the computational complexity is largely reduced. Experiment results on the acute leukaemia data are provided to verify the proposed method.

关键词：癌症弹性网络基因组计算方法

Huberized Multiclass Support Vector Machine for Microarray Classification

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自动化学报 2010年第3期36卷 399-405页

作者： LI Jun-Tao jia ying-min The Seventh Research Division Beihang University Beijing100191 P. R. China

This paper proposes a new multiclass support vector machine(SVM)for simultaneous gene selection and microarray classification. Combining the huberized hinge loss function and the elastic net penalty, the proposed SVM can perform automatic gene selection and encourages a grouping effect. The coefficient paths of the proposed SVM are shown to be piecewise linear with respect to the single regularization parameter, based on which the solution path algorithm is developed with low computational complexity. Experiments performed on the leukemia data set are provided to verify the obtained results

关键词：基因支持向量机计算方法路径系数

Cloning and Sequence Analysis of a Novel Cold-Adapted Lipase Gene from Strain lip35 (Pseudomonas sp.)

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Agricultural Sciences in China 2008年第10期7卷 1216-1221页

作者： WANG Cai-hong GUO Run-fang YU Hong-wei jia ying-min Department of Biology Engineering Agricultural University of Hebei Baoding 071000 P.R.China

A combination method of the usual-PCR and reverse-PCR for the cloning of a novel lipase gene directly from the total genomic DNA of strain lip35 （Pseudomonas sp.） is described, whereby a lipase gene （lip） was cloned directly from genomic DNA. The sequence data have been deposited in the GenBank and EMBL data bank with the accession number EU414288. The nucleotide sequence showed a major open reading frame encoding a 59-kDa protein of 566 amino acid residues, which contained a lipase consensus sequence GXSXG. The lipase lip had 74 and 70% homologies with the lipases of an uncultured bacterium and P. fluorescens PfO-1, respectively, but it did not show any overall homology with lipases from other origins. The functional lipase was obtained when the lip gene was expressed in Pichia pastoris GS115.

关键词： PCR reverse-PCR lipase gene clone sequence analysis

Improved Robust Adaptive Control of a Fluidized Bed Combustor for Sewage Sludge

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自动化学报 2005年第4期 70-74页

作者： MENG Hong-Xia jia ying-min(The Seventh Research Division, Beijing University of Aeronautics and Astronautics, Beijing 100083)

关键词： Robust adaptive control ?uidized bed combustor sewage sludge combustion timedelay

Stability Analysis of Continuous-time Iterative Learning Control Systems with Multiple State Delays

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自动化学报 2010年第5期36卷 696-703页

作者： MENG De-Yuan jia ying-min DU Jun-Ping YU Fa-Shan The Seventh Research Division Beihang University (BUAA) Beijing 100083 P. R. China Beijing Key Laboratory of Intelligent Telecommunications Software and Multimedia School of Computer Science and Technology Beijing University of Posts and Telecommunications Beijing 100876 P.R. China School of Electrical Engineering and Automation Henan Polytechnic University Jiaozuo 454000 P. R. China

This paper presents a stability analysis of the iterative learning control(ILC)problem for continuous-time systems with multiple state delays, especially when system parameters are subject to polytopic-type uncertainties. Using the two-dimensional(2-D) analysis approach to ILC, the continuous-discrete Roesser s type linear systems are employed to describe the entire learning dynamics of time-delay systems(TDS)with the development of an expanding operator. Based on such Roesser systems, the 2-D system theory is first used to develop a necessary and suffcient condition for the asymptotic stability of ILC, and then the robust H∞control theory is combined to provide a suffcient condition in terms of linear matrix inequalities(LMIs)for the monotonic convergence of *** is shown that learning gains can be determined by solving LMIs, which ensure the control input error converges monotonically to zero as a function of iteration. Simulation results show that a robust asymptotically stable ILC scheme can become robustly monotonically convergent by adding the P-type learning gains that satisfy a set of LMIs, which can also improve the convergence rate greatly

关键词：连续系统稳定性自动化 TDS

非合作目标绕飞任务的航天器鲁棒姿轨耦合控制

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控制理论与应用 2018年第10期35卷 1405-1414页

作者：黄艺贾英民北京航空航天大学第七研究室北京100191

本文研究了非合作目标强迫绕飞过程中存在外部干扰和参数不确定性以及控制输入饱和约束下的航天器鲁棒姿轨耦合控制问题.首先,根据视线坐标系下的轨道动力学方程和本体坐标系下的姿态动力学方程,建立了满足视线指向要求的航天器相对姿... 详细信息

本文研究了非合作目标强迫绕飞过程中存在外部干扰和参数不确定性以及控制输入饱和约束下的航天器鲁棒姿轨耦合控制问题.首先,根据视线坐标系下的轨道动力学方程和本体坐标系下的姿态动力学方程,建立了满足视线指向要求的航天器相对姿轨耦合动力学模型.其次,针对姿轨耦合模型,基于反步法设计了具有抗饱和能力的鲁棒自适应姿轨耦合控制律,其中利用抗饱和技术设计了饱和补偿器,并结合自适应方法对未知参数和扰动上界进行估计,并基于Lyapunov方法给出了闭环系统的稳定性证明.最后,将提出的控制方案进行了数值仿真和比较,验证了其有效性.

关键词：非合作绕飞视线指向姿轨耦合输入饱和鲁棒控制