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ADP-dependent glucokinase controls metabolic fitness in prostate cancer progression

Military Medical Research 2024年第5期11卷 643-662页

作者： Hang Xu Yi-Fan Li Xian-Yan-Ling Yi xiao-Nan Zheng Yang Yang Yan Wang Da-Zhou Liao jia-peng Zhang Ping Tan Xing-Yu Xiong Xi Jin Li-Na Gong Shi Qiu De-Hong Cao Hong Li Qiang Wei Lu Yang jian-Zhong Ai Department of Urology West China HospitalSichuan UniversityChengdu610041China Institute of Urology West China HospitalSichuan UniversityChengdu610041China Animal Experimental Center West China HospitalSichuan UniversityChengdu610041China Research Core Facility West China HospitalSichuan UniversityChengdu610041China

Background Cell metabolism plays a pivotal role in tumor progression,and targeting cancer metabolism might effectively kill cancer *** aimed to investigate the role of hexokinases in prostate cancer(PCa)and identify a crucial target for PCa *** The Cancer Genome Atlas(TCGA)database,online tools and clinical samples were used to assess the expression and prognostic role of ADP-dependent glucokinase(ADPGK)in *** effect of ADPGK expression on PCa cell malignant phenotypes was validated in vitro and in *** proteomics,metabolomics,and extracellular acidification rate(ECAR)and oxygen consumption rate(OCR)tests were performed to evaluate the impact of ADPGK on PCa *** underlying mechanisms were explored through ADPGK overexpression and knockdown,co-immunoprecipitation(Co-IP),ECAR analysis and cell counting kit-8(CCK-8)*** ADPGK was the only glucokinase that was both upregulated and predicted worse overall survival(OS)in prostate adenocarcinoma(PRAD).Clinical sample analysis demonstrated that ADPGK was markedly upregulated in PCa tissues ***-PCa *** ADPGK expression indicates worse survival outcomes,and ADPGK serves as an independent factor of biochemical *** vitro and in vivo experiments showed that ADPGK overexpression promoted PCa cell proliferation and migration,and ADPGK inhibition suppressed malignant ***,proteomics,and ECAR and OCR tests revealed that ADPGK significantly accelerated glycolysis in ***,ADPGK binds aldolase C(ALDOC)to promote glycolysis via AMP-activated protein kinase(AMPK)*** was positively correlated with ADPGK,and high ALDOC expression was associated with worse survival outcomes in *** In summary,ADPGK is a driving factor in PCa progression,and its high expression contributes to a poor prognosis in PCa *** accelerates PCa glycolysis and progression by activating ALDOC-AMPK signaling,suggesting

关键词： Prostate cancer(PCa) ADP-dependent glucokinase(ADPGK) Aldolase C(ALDOC) AMPK Glycolysis

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Retinal vascular diameter changes assessed with a computer-assisted software after strabismus surgery

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International Journal of Ophthalmology(English edition) 2020年第4期13卷 620-624页

作者： Jin-Qiong Zhou Jing Fu Ji-peng Li xiao-Zhen Wang Wen-Ying Wang Bo-Wen Zhao Meng Qi Beijing Tongren Eye Center Beijing Tongren HospitalCapital Medical UniversityBeijing Ophthalmology&Visual Sciences Key LaboratoryBeijing 100730China

AIM: To quantitatively investigate the retinal vascular diameter changes, analyzing the early and long-term effects on the retinal circulation, with 6-month follow-up. METHODS: Patients underwent horizontal strabismus surger y were enrolled prospectively. Retinal vessel diameters on color fundus photographs were assessed before and 1, 7 d, 6 mo after surgery, using a computerassisted quantitative assessment software. To evaluate the retinal vascular caliber changes, retinal vascular diameters were calculated by means of the Parr–Hubbard formula as the central retinal arteriolar equivalent(CRAE) and central retinal venular equivalent(CRVE). The arteriovenous ratio(AVR) was calculated as CRAE divided by CRVE. RESULTS: A total of 154 eyes of 104 consecutive patients were included. Compared with the data before surgery(121.55±24.67), the mean CRAE(131.18±28.29) significantly increased 1 d af ter surger y(P=0.003), but went back to baseline level at 7 d(118.89±30.35, P=0.15), and 6 mo(123.22±15.32, P=0.60), so did the AVR(P<0.001, P=0.08, P=0.07). As for the mean CRVE, there was no significant difference between those four time points(172.43±33.25, 175.57±36.98, 174.03±40.18, 174.86±20.46, P=1.00). CONCLUSION: Strabismus surgery on both lateral and media rectus muscles, or single media rectus muscle may increase retinal blood flow during the early postoperative period, but would return to normal later. The number of transected anterior ciliary arteries rather might be the main cause of retinal hemodynamic changes early after strabismus surgery.

关键词： retinal blood flow retinal vascular diameter strabismus strabismus surgery

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航天器输入受限的鲁棒自适应姿态跟踪控制

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控制与决策 2021年第9期36卷 2297-2304页

作者：孙亮马佳鹏北京科技大学自动化学院北京100083 北京科技大学人工智能研究院北京100083

研究含模型不确定性的刚性航天器输入受限时的姿态跟踪控制设计问题.针对修改的罗德里格斯姿态参数描述的航天器姿态跟踪动力学模型,基于一种有界非线性连续函数和修改的罗德里格斯姿态参数自身有界性,设计鲁棒自适应状态反馈受限控制器... 详细信息

研究含模型不确定性的刚性航天器输入受限时的姿态跟踪控制设计问题.针对修改的罗德里格斯姿态参数描述的航天器姿态跟踪动力学模型,基于一种有界非线性连续函数和修改的罗德里格斯姿态参数自身有界性,设计鲁棒自适应状态反馈受限控制器,不确定参数的自适应更新律可保证在线估计参数的有界性.通过所提出的输入受限控制设计方法给出输入受限幅值、期望轨迹上界、模型不确定性上界与控制器增益之间的定量关系,并采用李亚普诺夫方法证明通过选择合适的控制器参数可以保证闭环系统角速度误差渐近收敛到零,且姿态跟踪误差收敛到原点小邻域,同时保证控制量始终在预先给定的受限范围内.仿真结果验证了所设计的控制器可在输入受限的情况下完成控制目标并有效抑制模型的不确定性影响.

关键词：航天器姿态跟踪控制输入受限状态反馈自适应控制稳定性

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海相沉积结构性软土一维大应变固结分析（英文）

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2020年第1期21卷 29-43页

作者： Chuan-xun LI Jin-yang xiao Wen-bing WU Guo-xiong MEI peng-peng NI Chin-jian LEO Department of Civil Engineering Jiangsu UniversityZhenjiang 212013China Engineering Research Centre of Rock-Soil Drilling&Excavation and Protection Ministry of EducationWuhan 430074China College of Civil Engineering and Architecture Guangxi UniversityNanning 530004China School of Computing Engineering and MathematicsUniversity of Western SydneySydney NSW2751Australia

目的:天然沉积的结构性软土分布广泛,但能考虑结构性影响的大应变固结理论鲜有报道。本文考虑结构屈服压力随初始有效应力的变化及土体结构性对压缩与渗透特性的影响,建立结构性软土的大应变固结模型。研究结构性及初始有效应力对大应... 详细信息

目的:天然沉积的结构性软土分布广泛,但能考虑结构性影响的大应变固结理论鲜有报道。本文考虑结构屈服压力随初始有效应力的变化及土体结构性对压缩与渗透特性的影响,建立结构性软土的大应变固结模型。研究结构性及初始有效应力对大应变固结性状的影响,并探讨结构性软土大、小应变固结性状的差异,以提高软土固结计算的精准度。创新点:1.建立考虑天然沉积软土结构性影响的一维大应变固结模型,且该模型能考虑结构屈服压力随初始有效应力的变化;2.分析天然结构性软土大、小应变固结性状的差异,为实际工程中的软土固结计算提供理论依据。方法:1.总结结构性软土对压缩和渗透特性的影响及结构屈服压力与初始有效应力间的关系;2.通过理论推导,构建考虑结构性影响的软体一维大应变固结模型(公式(15)和(16));3.通过对模型进行数值求解,分析软土结构性对大应变固结性状的影响,以及考虑结构性影响的大、小应变固结性状的差异。结论:1.大应变假定下结构性软土中超静孔压的消散速率要比小应变假定下快,且这种差异随着土层应变增大而增大;当应变值超过15%时必须采用大应变假定。2.如果土层的初始有效应力计算方法相同,则大、小应变不同假定下土层的最终沉降值是相同的。3.相同几何假定下,初始有效应力计算方法对超静孔压消散速率几乎无影响,但对沉降变形影响明显。4.大、小应变假定下固结性状间的差异随结构屈服压力的增大而减小。

关键词：结构性软土大应变固结结构屈服压力有限差分法

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Total and cause-specific mortality attributable to cold spells in China:A multicity and multicounty study

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Advances in Climate Change Research 2023年第6期14卷 827-835页

作者： He-jia SONG Yi-Bin CHENG Yu WANG Yu-Shu HUANG Yan WANG Xue-Mei SU Shilu TONG Yong-Hong LI xiao-Yuan YAO National Institute of Environmental Health Chinese Center for Disease Control and PreventionBeijing 100021China School of Public Health Institute of Environment and Population HealthAnhui Medical UniversityHefei 230032China Center for Global Health School of Public HealthNanjing Medical UniversityNanjing 211166China School of Public Health and Social Work Queensland University of TechnologyBrisbane 4000Australia Daxing Center for Disease Control and Prevention Beijing 102600China Department of Infectious Diseases Control and Prevention Jiaxing Center for Disease Control and PreventionJiaxing 314050China

Climate change has resulted in an increase in the frequency and intensity of extreme cold weather events,but few multicity or multicounty researches have explored the association between cold spells and mortality risk and *** collected daily data on climate,sociodemo-graphic factors and mortality in 18 cities/counties across 11 geographical regions for the period of November to March 2014-2018.A distributed lag nonlinear model was used to examine the association between cold spells and mortality after adjustment for confounding *** definitions of cold spells were ***-meta regression analysis was applied to pool the impacts over different *** spells were significantly associated with all-cause mortality at lag 0-21(CRR:1.38,95%CI:1.21,1.57).In addition to respiratory diseases and cir-culatory system diseases,digestive,endocrine and nervous system diseases and injury were also affected by cold *** magnitude of the impacts of cold spells on mortality varied among the diseases investigated,with the highest risk estimate found for influenza and pneumonia(CRR:2.00,95%CI:1.45,2.76)and the lowest estimate found for injury(CRR:1.26,95%CI:1.09,1.46).The fraction of all-cause mortality attributable to cold spells was 2.31%(95%CI:0.90%,3.46%).Among the regional differences,the attributable burden of all-cause mortality was higher in rural areas and subtropical monsoon climate zone,with attributable fractions of 2.85%(95%CI:1.23%,4.11%)and 3.36%(95%CI:0.55%,5.35%),*** spells increased mortality from a range of ***,older adults and residents of rural areas and subtropical monsoon climate zone were more vulnerable to cold spells *** findings may help to formulate preventive strategies and early warning response plans to reduce mortality burden of extreme cold events.

关键词： Cold spells Distributed lag nonlinear model Attributable fraction Mortality burden Sensitive diseases

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脉冲测量雷达卫星标校的实现

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系统工程与电子技术 2007年第10期29卷 1600-1602页

作者：黄家贵杨潇北京跟踪与通信技术研究所北京100094

针对轴系误差标定是脉冲测量雷达使用维护的重要内容,是确保其测量精度的主要手段。介绍了脉冲测量雷达轴系误差的卫星标定方法,给出了标校模型和一种基于最速下降法的迭代算法,结合某雷达的精度鉴定进行了应用研究。结果表明,卫星标定... 详细信息

针对轴系误差标定是脉冲测量雷达使用维护的重要内容,是确保其测量精度的主要手段。介绍了脉冲测量雷达轴系误差的卫星标定方法,给出了标校模型和一种基于最速下降法的迭代算法,结合某雷达的精度鉴定进行了应用研究。结果表明,卫星标定方法完全可行,可以避免常规标定方法所涉及的大量人工参与,标校结果更为客观、真实、可信,并分析了卫星标校方法的使用条件。

关键词：雷达卫星标定迭代

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Calpain-2 activity promotes aberrant endoplasmic reticulum stress-related apoptosis in hepatocytes

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World Journal of Gastroenterology 2020年第13期26卷 1450-1462页

作者： Ru-jia Xie xiao-Xia Hu Lu Zheng Shuang Cai Yu-Si Chen Yi Yang Ting Yang Bing Han Qin Yang Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases College of Basic Medical SciencesGuizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina Department of Pathophysiology College of Basic Medical SciencesGuizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina Department of Physiology College of Basic Medical SciencesGuizhou Medical UniversityGuiyang 550025Guizhou ProvinceChina

BACKGROUND Calpain-2 is a Ca^2+-dependent cysteine protease,and high calpain-2 activity can enhance apoptosis mediated by multiple *** To investigate whether calpain-2 can modulate aberrant endoplasmic reticulum(ER)stress-related apoptosis in rat hepatocyte BRL-3A *** BRL-3A cells were treated with varying doses of dithiothreitol(DTT),and their viability and apoptosis were quantified by 3-[4,5-dimethyl-2-thiazolyl]-2,5-diphenyl-2-H-tetrazolium bromide and flow *** expression of ER stress-and apoptosis-related proteins was detected by Western blot *** protease activity of calpain-2 was determined using a fluorescent substrate,*** Ca^2+content,and ER and calpain-2 co-localization were characterized by fluorescent *** impact of calpain-2 silencing by specific small interfering RNA on caspase-12 activation and apoptosis of BRL-3A cells was *** DTT exhibited dose-dependent cytotoxicity against BRL-3A cells and treatment with 2 mmol/L DTT triggered BRL-3A cell *** treatment significantly upregulated 78 kDa glucose-regulated protein,activating transcription factor 4,C/EBP-homologous protein expression by>2-fold,and enhanced PRKR-like ER kinase phosphorylation,caspase-12 and caspase-3 cleavage in BRL-3A cells in a trend of *** treatment also significantly increased intracellular Ca^2+content,calpain-2 expression,and activity by>2-fold in BRL-3A ***,immunofluorescence revealed that DTT treatment promoted the ER accumulation of ***,calpain-2 silencing to decrease calpain-2 expression by 85%significantly mitigated DTT-enhanced calpain-2 expression,caspase-12 cleavage,and apoptosis in BRL-3A *** The data indicated that Ca^2+-dependent calpain-2 activity promoted the aberrant ER stress-related apoptosis of rat hepatocytes by activating caspase-12 in the ER.

关键词： Calcium Calpain-2 Caspase-12 Endoplasmic reticulum stress Apoptosis Hepatocyte

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Green tea polyphenol, epigallocatechin-3-gallate, possesses the antiviral activity necessary to fight against the hepatitis B virus replication in vitro

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第6期15卷 533-539页

作者： Jing-yao PANG Kui-jun ZHAO jia-bo WANG Zhi-jie MA xiao-he xiao Clinical Pharmacy Laboratory Beijing Friendship Hospital Capital Medical University China Military Institute of Chinese Medicine 302 Military Hospital Integrative Medicine Center 302 Military Hospital

Although several antiviral drugs and vaccines are available for use against hepatitis B virus （HBV）, hepatitis caused by HBV remains a major public health problem worldwide, which has not yet been resolved, and new anti-HBV drugs are in great demand. The present study was performed to investigate the anti-HBV activity of epigallocatechin- 3-gallate （EGCG）, a natural-origin compound, in HepG2 2.2.15 cells. The antiviral activity of EGCG was examined by detecting the levels of HBsAg and HBeAg in the supematant and extracellular HBV DNA. EGCG effectively suppressed the secretion of HBsAg and HBeAg from HepG2 2.2.15 cells in a dose- and time-dependent manner, and it showed stronger effects at the level of 0.11-0.44 pmol/ml （50-200 μg/ml） than lamivudine （3TC） at 0.87 μmol/ml （200 pg/ml）. EGCG also suppressed the amount of extracellular HBV DNA. The data indicated that EGCG possessed anti-HBV activity and suggested the potential of EGCG as an effective anti-HBV agent with low toxicity.

关键词： Epigallocatechin-3-gallate Hepatitis B virus (HBV) Antiviral activity HepG2 2.2.15 Lamivudine

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多轴循环塑性双界面本构模型研究

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建筑结构学报 2020年第11期41卷 160-165,175页

作者：贾君玉肖魁同济大学土木工程学院上海200092 上海建筑设计研究院有限公司上海200041 上海建筑空间结构工程技术研究中心上海200041

地震作用具有很大的随机性和不确定性,为准确描述结构在强震作用下的弹塑性反应,基于循环塑性理论框架,提出了改进的双界面本构模型,对屈服面及边界面的平移运动和变形演化方程进行改进,并通过在塑性应变空间中引入一个记忆面来考虑材... 详细信息

地震作用具有很大的随机性和不确定性,为准确描述结构在强震作用下的弹塑性反应,基于循环塑性理论框架,提出了改进的双界面本构模型,对屈服面及边界面的平移运动和变形演化方程进行改进,并通过在塑性应变空间中引入一个记忆面来考虑材料对应变加载历史的硬化记忆效应。模型参数均可通过单轴拉伸及单轴滞回试验曲线标定。研究结果表明:该模型能准确地预测多轴任意路径加载下多晶体金属材料的屈服平台段、Bauschinger效应、循环硬化、棘轮效应、记忆效应等行为;将预测结果与各类金属及合金材料的试验结果进行对比,二者的最大正应力、剪应力的预测误差分别在2%、4%以内,验证了模型的适用性,其可为精细化弹塑性分析提供理论依据。

关键词：钢材铝合金本构模型循环塑性双界面模型

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地面沉降对地下水时空响应及其非工程控沉

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东北大学学报（自然科学版） 2021年第7期42卷 1032-1040页

作者：贾超张少鹏丁朋朋吴静山东大学海洋研究院山东青岛266237

针对潍北平原地面沉降问题,建立地面沉降流固耦合三维数值模型,从宏观的角度探讨潍北平原在未来一段时间内区域性地面沉降的发展规律,考虑到地下水开采与地面沉降的相互关系,量化地下水开采对其的负面影响.研究结果表明:潍北平原地区沉... 详细信息

针对潍北平原地面沉降问题,建立地面沉降流固耦合三维数值模型,从宏观的角度探讨潍北平原在未来一段时间内区域性地面沉降的发展规律,考虑到地下水开采与地面沉降的相互关系,量化地下水开采对其的负面影响.研究结果表明:潍北平原地区沉降漏斗中心主要分布在寿光市营里镇东部和广陵乡北部;保持2019年地下水开采量,潍北平原未来10年内的沉降仍呈继续发展和增加态势,2029年最大累积地面沉降量将高达565.42 mm,年均增长率为38.97 mm/a,沉降中心未发生转移;随着地下水开采量的减少,地面沉降发育程度明显得到缓解,随之持续减小,地面沉降的减缓趋势则会逐渐趋于平缓;当减采量控制在10%与30%之间时,地面沉降灾害的减缓效果较佳.

关键词：潍北平原地面沉降地下水开采数值模型演化趋势

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