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Science China Chemistry 2025年第1期 360-368页

作者： Zheng liu Changyu Zhang Zipeng Li Wanying Ma jia liu Xiang Xia Ning Xu Wen Sun jianjun Du jiangli Fan Xiaojun Peng State Key Laboratory of Fine Chemicals Dalian University of Technology Ningbo Institute of Dalian University of Technology

Endoplasmic reticulum(ER) viscosity has emerged as a new potential biomarker for hepatic fibrosis(HF). Herein, we report the first ER-targeting viscosity-responsive near-infrared(NIR) fluorescent probe HBT-PP for investigations of HF *** twisted intramolecular charge transfer(TICT) and the excited state intramolecular proton transfer(ESIPT) are combined to endow HBT-PP with the selective response towards viscosity(～62-fold) and a super large stokes shift(～320 nm). HBT-PP mainly targets organelle ER and is able to image the drug-or alcohol-induced viscosity elevation in living cells. Significantly,HBT-PP was successfully applied to visualize the gender difference in HF and the protective role of estrogen in restraining HF progression. Of note, after 10 min of stain with HBT-PP, HF lesions showed a stage-based response, indicating the potential of this probe in fast and early diagnosis of HF. These results clearly demonstrated the promising application of HBT-PP as a new tool for exploring ER viscosity-involved physiological and pathological processes.

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Decreased levels of phosphorylated synuclein in plasma are correlated with poststroke cognitive impairment

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Neural Regeneration Research 2025年第9期20卷 2598-2610页

作者： Yi Wang Yuning Li Yakun Gu Wei Ma Yuying Guan Mengyuan Guo Qianqian Shao Xunming Ji jia liu Department of Clinical Laboratory Beijing Bo’ai HospitalChina Rehabilitation Research CenterCapital Medical UniversityBeijingChina Beijing Institute of Brain Disorders Laboratory of Brain DisordersMinistry of Science and TechnologyCollaborative Innovation Center for Brain DisordersBeijing Advanced Innovation Center for Big Data-based Precision MedicineCapital Medical UniversityBeijingChina Department of Neurosurgery Xuanwu HospitalCapital Medical UniversityBeijingChina

Poststro ke cognitive impairment is a major secondary effect of ischemic stroke in many patients;however,few options are available for the early diagnosis and treatment of this *** aims of this study were to(1)determine the specific relationship between hypoxic andα-synuclein during the occur of poststroke cognitive impairment and(2)assess whether the serum phosphorylatedα-synuclein level can be used as a biomarker for poststro ke cognitive *** found that the phosphorylatedα-synuclein level was significantly increased and showed pathological aggregation around the cerebral infa rct area in a mouse model of ischemic *** addition,neuronalα-synuclein phosphorylation and aggregation were observed in the brain tissue of mice subjected to chronic hypoxia,suggesting that hypoxia is the underlying cause ofα-synuclein-mediated pathology in the brains of mice with ischemic *** phosphorylatedα-synuclein levels in patients with ischemic stroke were significantly lower than those in healt hy subjects,and were positively correlated with cognition levels in patients with ischemic ***,a decrease in serum high-density lipoprotein levels in stroke patie nts was significantly correlated with a decrease in phosphorylatedα-synuclein *** ischemic stroke mice did not show significant cognitive impairment or disrupted lipid metabolism 14 days after injury,some of them exhibited decreased cognitive function and reduced phosphorylatedα-synuclein *** together,our results suggest that serum phosphorylatedα-synuclein is a potential biomarker for poststroke cognitive impairment.

关键词： biomarker high-density lipoprotein ischemic stroke phosphorylatedα-synuclein poststroke cognitive impairment

负载骨形态发生蛋白2水凝胶诱导牙髓干细胞的成骨分化

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中国组织工程研究 2025年第16期29卷 3301-3310页

作者：伊斯拉尔古丽·麦麦提贾森刘佳新疆医科大学第一附属医院(附属口腔医院)儿童口腔科-口腔预防科新疆维吾尔自治区乌鲁木齐市830054 西安医学院口腔医学院陕西省西安市710021 新疆维吾尔自治区口腔医学研究所新疆维吾尔自治区乌鲁木齐市830054

背景:前期研究证明,甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶支架可促进人牙髓干细胞的增殖及分化。水凝胶负载骨形态发生蛋白2被认为是骨修复中有前景的材料。目的:观察负载不同质量浓度骨形态发生蛋白2的甲基丙烯酸酐改性明... 详细信息

背景:前期研究证明,甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶支架可促进人牙髓干细胞的增殖及分化。水凝胶负载骨形态发生蛋白2被认为是骨修复中有前景的材料。目的:观察负载不同质量浓度骨形态发生蛋白2的甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶对人牙髓干细胞成骨向分化的诱导作用。方法:制备含0,50,100,200μg/mL骨形态发生蛋白2的甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶,分别记为GelMA/TDM、BMP-2(50 ng/mL)GelMA/TDM、BMP-2(100 ng/mL)GelMA/TDM、BMP-2(200 ng/mL)GelMA/TDM,检测复合水凝胶对骨形态发生蛋白2的体外缓释性能。采用改良组织块酶消化法提取人牙髓干细胞,分别接种于4种水凝胶表面,采用CCK-8法检测细胞增殖,DAPI染色检测细胞黏附;对各组水凝胶表面的人牙髓干细胞进行成骨诱导,进行碱性磷酸酶染色、碱性磷酸酶活性检测与茜素红染色,采用RT-PCR法检测相关成骨基因(Runx2、骨形态发生蛋白2、骨桥蛋白、骨钙素、Ⅰ型胶原)表达。结果与结论:①甲基丙烯酸酐改性明胶/经处理牙本质基质复合水凝胶可持续释放骨形态发生蛋白2长达21 d,在第3-6天释放较快,第6天之后释放趋于平稳;②4种水凝胶均可促进人牙髓干细胞的增殖,其中以BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶的作用最明显;相较于GelMA/TDM复合水凝胶,BMP-2-GelMA/TDM复合水凝胶可促进人牙髓干细胞的黏附,其中以BMP-2(200 ng/mL)-GelMA/TDM复合水凝胶的作用最明显;③相较于GelMA/TDM复合水凝胶,BMP-2-GelMA/TDM复合水凝胶可提升碱性磷酸酶活性、钙结节含量与相关成骨基因表达,综合分析显示BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶的作用更明显;④结果表明,BMP-2(100 ng/mL)-GelMA/TDM复合水凝胶促进牙髓干细胞成骨向分化的能力更明显。

关键词：人牙髓干细胞骨形态形成蛋白2 细胞增殖细胞分化组织工程牙髓-牙本质再生

光交联复合水凝胶支架中人牙髓干细胞的成骨/成牙分化能力

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中国组织工程研究 2025年第19期29卷 4022-4028页

作者：杨杜娟程梦可刘佳新疆医科大学第一附属医院(附属口腔医院)儿童口腔科-口腔预防科新疆维吾尔自治区乌鲁木齐市830054 新疆维吾尔自治区口腔医学研究所新疆维吾尔自治区乌鲁木齐市830054

背景:甲基丙烯酸酐改性明胶(gelatin-methacryloyl,Gel-MA)与经处理牙本质基质(treated dentin matrix,TDM)在一定比例下经过紫外线交联后形成的复合水凝胶支架具有良好的孔隙率、机械性能、溶胀性能及生物降解率,这为临床上年轻恒牙的... 详细信息

背景:甲基丙烯酸酐改性明胶(gelatin-methacryloyl,Gel-MA)与经处理牙本质基质(treated dentin matrix,TDM)在一定比例下经过紫外线交联后形成的复合水凝胶支架具有良好的孔隙率、机械性能、溶胀性能及生物降解率,这为临床上年轻恒牙的牙髓再生提供了新的思路和方法。目的:探究1∶2质量比Gel-MA/TDM复合光交联水凝胶支架对人牙髓干细胞增殖能力及成骨/成牙本质分化能力的影响。方法:将第3代牙髓干细胞接种至1∶2质量比Gel-MA/TDM复合水凝胶支架中,采用CCK-8实验检测人牙髓干细胞在复合水凝胶支架中的增殖能力。将第3代牙髓干细胞接种至1∶2质量比Gel-MA/TDM复合水凝胶支架中并进行成骨诱导,采用碱性磷酸酶染色及茜素红染色观察矿化结节的形成情况,采用RT-PCR检测成牙相关因子(牙本质基质蛋白1、牙本质涎磷蛋白)和成骨相关因子(骨钙素、Runt相关转录因子2)的基因表达。结果与结论:①CCK-8检测结果显示,前7 d的牙髓干细胞增殖能力明显升高,第10天时速度减缓;②碱性磷酸酶染色及茜素红染色结果显示,Gel-MA/TDM复合水凝胶组牙髓干细胞的碱性磷酸酶活性及矿化结节生成强于单纯Gel-MA水凝胶组(P<0.05);③RT-PCR结果显示,Gel-MA/TDM复合水凝胶组牙髓干细胞中牙本质基质蛋白1、牙本质涎磷蛋白、骨钙素、Runt相关转录因子2的基因表达量明显高于单纯Gel-MA水凝胶组(P<0.05),且14 d基因表达量明显高于7 d(P<0.05)。结果表明,培养在1∶2 Gel-MA/TDM复合水凝胶支架上的牙髓干细胞表现出较好的增殖能力,能够强化牙髓干细胞的成骨、成牙本质分化能力。

关键词：牙髓再生甲基丙烯酸酐改性明胶牙本质基质复合支架牙髓干细胞成骨成牙本质

基于超声反冲洗过滤器过滤含油污水的试验研究

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煤炭技术 2025年第1期44卷 269-272页

作者：贾利军刘佳新疆天业汇合新材料有限公司新疆石河子832000 宁波安泰环境化工工程设计有限公司浙江宁波315048

针对含油污水处理,进行了基于超声波辅助反冲洗过滤的试验研究,试验考察了滤芯初试压差、无超声/超声反冲洗时间对滤芯压差的影响、超声功率密度对滤芯反冲洗压差的影响、不同反冲流量下,超声反冲时间对反冲过滤液中含油量的影响、滤料... 详细信息

针对含油污水处理,进行了基于超声波辅助反冲洗过滤的试验研究,试验考察了滤芯初试压差、无超声/超声反冲洗时间对滤芯压差的影响、超声功率密度对滤芯反冲洗压差的影响、不同反冲流量下,超声反冲时间对反冲过滤液中含油量的影响、滤料超声反冲洗再生能力、无超声/超声过滤滤芯反冲洗含油量对比等因素。通过该实验研究结果表明,超声辅助反冲洗过滤含油和聚合物污水效果良好,在反冲洗流量为65 L/min、反冲洗超声功率密度为1.7 W/cm^(2)、反冲时间17 min时,超声辅助反冲洗滤芯过滤效率较无超声作用滤芯过滤效率高,其滤芯过滤效率分别为93.81%、43.50%。

关键词：含油污水超声波反冲洗过滤器

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人因工程学影响下的办公街区设计实践

城市建筑 2025年第01期22卷 60-65页

作者：陈冰刘佳浙江大学建筑设计研究院有限公司

随着城市化进程的加速推进，以及办公环境对员工舒适性和效率要求的不断提高，办公街区设计成为一个备受关注的领域。人因工程学作为一门研究人类与技术、环境之间相互作用关系的学科，可以为办公街区设计提供重要的指导和支持。文章通... 详细信息

随着城市化进程的加速推进，以及办公环境对员工舒适性和效率要求的不断提高，办公街区设计成为一个备受关注的领域。人因工程学作为一门研究人类与技术、环境之间相互作用关系的学科，可以为办公街区设计提供重要的指导和支持。文章通过对人因工程学在办公街区设计实践中的应用综述，分析了人因工程学对办公街区各个尺度设计的影响。合理运用人因工程学原理进行嘉兴国际金融广场的项目实践，为打造嘉兴金融区提供有益思路。

关键词：人因工程学办公街区尺度非正式交流空间共享空间多维空间

Spreading Speeds of Time-Dependent Partially Degenerate Reaction-Diffusion Systems

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Chinese Annals of Mathematics,Series B 2022年第1期43卷 79-94页

作者： jia liu School of Science Chang'an UniversityXi'an 710064China

This paper is concerned with the spreading speeds of time dependent partially degenerate reaction-diffusion systems with monostable *** using the principal Lyapunov exponent theory,the author first proves the existence,uniqueness and stability of spatially homogeneous entire positive solution for time dependent partially degenerate reaction-diffusion *** the author shows that such system has a finite spreading speed interval in any direction and there is a spreading speed for the partially degenerate system under certain *** author also applies these results to a time dependent partially degenerate epidemic model.

关键词： Partially degenerate Reaction-diffusion system Time dependent Spreading speed

A Dynamic Multi-Player Bargaining Game with Veto Players

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Journal of Systems Science & Complexity 2021年第2期34卷 673-691页

作者： liu jia WANG Xianjia School of Economics and Management Wuhan UniversityWuhan 430072China Institute of Systems Engineering Wuhan UniversityWuhan 430072China

This paper studies the effect of veto right on players’income in multi-player dynamic bargaining *** on a basic multi-person dynamic bargaining model generalized by the Rubinstein’s two-person alternating-offer bargaining model,the authors construct a dynamic multi-player bargaining game with veto players by adding a constraint to its negotiation process,which is obtained by studying the influence of exercising the veto right exercised by veto *** authors emphatically describe the strategic game form of this dynamic bargaining game and study its equilibrium,then we analyze the relationship between the minimum acceptable payoff of the veto players and the equilibrium *** research shows that veto right may increase the benefits of veto players and decrease the benefits of non-veto *** players will not affect the players’benefits and the form of equilibrium when the minimum acceptable payoff of every veto player is relatively *** the minimum acceptable payoff of the veto player is high enough,he can only get the minimum acceptable payoff,and his benefit increases as his minimum acceptable payoff *** this case,the veto player has intention to obtain more resources by presenting a higher minimum acceptable payoff.

关键词： Dynamic bargaining game game theory multi-player game veto players

Analytical Solution of Thermocapillary Flow in Cylindrical Annuli with Curved Free Surface

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Journal of Thermal Science 2020年第1期29卷 260-267页

作者： liu jia PENG Lan Key Laboratory of Low-Grade Energy Utilization Technologies and Systems of Ministry of Education College of Energy and Power Engineering Chongqing University

In this paper we consider thermocapillary flow with interface deformation in cylindrical annuli subjected to lateral temperature *** on the assumption that Reynolds,Marangoni and capillary numbers are very small,we expanded the governing equations in terms of a small parameter,and the zeroth-order one is the Stokes problem,which can be analytically solved by use of separation of variables *** are obtained for the zeroth-order fluid and thermal fields as well as the first-order interfacial ***,temperature distributions and interface shapes are presented and the influence of aspect ratios is investigated.

关键词： thermocapillary flow analytical solution interface deformation

An electrochemical machining method for aeroengine blades based on four-directional synchronous feeding of cathode tools

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Chinese Journal of Aeronautics 2023年第9期36卷 380-391页

作者： jia liu Libing HUI Dongqian jia Yan liu Di ZHU College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China

Blades are critical components of modern *** the many characteristic structures of the blade,the leading/trailing edges are key structures that have the greatest influence on the aerodynamic effect and power conversion of the *** machining(ECM)is regarded as one of the most important techniques in blade manufacturing due to its process characteristics of high material removal rates,virtually no tool wear,and no areas of thermal or mechanical damage to the workpiece rim ***,an ECM method based on the fourdirectional synchronous feeding of four cathode tools is proposed to improve the machining accuracy of the leading and trailing edges of the *** blade ECM using this method,four cathode tools feed toward the basin/back surfaces and leading/trailing edges *** dynamic processing simulation and flow field simulation results of the ECM process show that the proposed method eliminates the sharp changes in the electric field and electrolyte flow field at the leading and trailing edges seen in traditional machining ***,the electric field and flow field stability of the leading and trailing edges at the final stage of machining is greatly *** comparison of the conventional and proposed ECM methods showed that four-directional synchronous feeding results in improved profile accuracy over repeated machining processes and good surface quality.

关键词： Aero-engine Blade Dynamic simulation Electrochemical machining Experiment