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sh(D)和OH(D)自由基基态的结构与势能函数

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物理学报 2009年第5期58卷 3047-3051页

作者：朱吉亮任廷琦王庆美曲阜师范大学物理工程学院曲阜273165

采用QCISD(T)/6-311++G(3df,2pd)和QCISD/6-311++G(3df,2pd)方法计算优化了sh(D)和OH(D)自由基分子基态X2Π的分子结构和离解能.并采用最小二乘法拟合Murrell-Sorbie函数得到了相应的势能函数,由此计算的振转常数与实验光谱数据符合得... 详细信息

采用QCISD(T)/6-311++G(3df,2pd)和QCISD/6-311++G(3df,2pd)方法计算优化了sh(D)和OH(D)自由基分子基态X2Π的分子结构和离解能.并采用最小二乘法拟合Murrell-Sorbie函数得到了相应的势能函数,由此计算的振转常数与实验光谱数据符合得相当好.

关键词： sh和OH自由基分子基态(X2Π) Murrell-Sorbie函数势能函数

抑制蛋白激酶C对口腔鳞癌细胞迁移和侵袭的影响

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上海口腔医学 2017年第2期26卷 171-174页

作者：陈钟徐勇纪晴厦门医学院福建厦门361023

目的 :利用舌癌细胞系HN4,探讨蛋白激酶C(protein kinase C,PKC)、P120-连环蛋白(P120-catenin,P120ctn)和E-钙黏蛋白(E-cadherin,E-cad)在口腔鳞癌中的相互关系及其在肿瘤细胞侵袭和转移中的作用机制。方法:采用质粒p GFP-V-RS-P120ctn sh RNA转染HN4,使HN4中P120ctn的表达显著降低,再使用星孢菌素(staurosporine,STS)抑制PKC的表达,进行PKC、P120ctn和E-cad m RNA和蛋白水平的检测;通过Transwell细胞侵袭及细胞迁移试验等方法,检测PKC被抑制前、后肿瘤细胞迁移和侵袭能力的变化。采用SPSS18.0软件包对数据进行统计学分析。结果:当PKC被STS抑制时,HN4/sh P120ctn细胞中P120ctn的表达显著升高,E-cad的表达也随之升高,肿瘤细胞的迁移和侵袭能力显著降低(P均<0.05)。结论:PKC可能参与了P120ctn和E-cad表达的调节,与细胞黏附的调控有关,进而在HN4细胞的迁移和侵袭过程中发挥作用。

关键词： P120-连环蛋白蛋白激酶C 口腔鳞状细胞癌

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筛选差异表达基因和蛋白质的方法进展

生物化学与生物物理进展 2001年第1期28卷 33-36页

作者：王吉村药立波赵忠良第四军医大学生物化学与分子生物学教研室西安710032

分离和鉴定差异表达基因和蛋白质不仅有助于发现基因和蛋白质的功能 ,更有助于揭示某些疾病的发生机理 .目前筛选差异表达基因的方法主要有差异显示PCR方法 (differentialdisplayRT PCR ,DDRT PCR)、消减杂交法 (subtractivehybridizati... 详细信息

分离和鉴定差异表达基因和蛋白质不仅有助于发现基因和蛋白质的功能 ,更有助于揭示某些疾病的发生机理 .目前筛选差异表达基因的方法主要有差异显示PCR方法 (differentialdisplayRT PCR ,DDRT PCR)、消减杂交法 (subtractivehybridization ,sh )、基因芯片技术 (DNAchiptechnique)和基因表达的系统分析 (serialanalysisofgeneexpression ,SAGE)等 ,其中消减杂交法中又先后建立了代表性差异分析技术 (representationaldifferenceanalysis ,RDA)、抑制消减杂交法 (suppressionsubtractivehybridization ,Ssh)和获得全长基因的消减杂交法 (full length gene obtainablesubtractivehybridization) .筛选差异表达蛋白质的方法主要有双向电泳技术(two dimentionalgelelectrophoresis)和噬菌体全套抗体库技术 (phagedisplayantibodyrepertoirelibrarytechnique) .这些方法各有特点 ,各有利弊 ,研究者可根据自己的需要选择适合于自己的方法 .

关键词：筛选差异表达基因差异表达蛋白质

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新医改下医疗服务质量控制体系运行效益研究

经济问题 2013年第5期 109-112页

作者：伍吉云肖凤井冈山大学医学院江西吉安343000

采用调查和文献研究法分析了新医改下医疗服务质量控制体系的运行效益。研究发现,该体系在减轻患者经济负担、提高患者满意度及促进医疗市场良性运转方面取得了初步成效,医院的病床一直处于满负荷使用状态,诊疗人次与出院人数显著增长,... 详细信息

采用调查和文献研究法分析了新医改下医疗服务质量控制体系的运行效益。研究发现,该体系在减轻患者经济负担、提高患者满意度及促进医疗市场良性运转方面取得了初步成效,医院的病床一直处于满负荷使用状态,诊疗人次与出院人数显著增长,医疗服务的可及性大为增强,医院的技术效率、规模效率和社会收益逐年提升。体现了管理模式的创新可提高医疗服务的质量和效益,但还存在医院内部管理机制不完善等问题,需进一步探索调动医务者积极性、强化长效监管和加强医院经济管理的对策和方法。

关键词：医疗服务质量医院管理新医改

论20世纪30年代中国话剧与电影的双向互动

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文化艺术研究 2017年第3期10卷 69-74页

作者：计敏上海戏剧学院上海200040

中国的话剧与电影在20世纪30年代发生了前所未有的交集与碰撞。在双向互动中,电影的发展产生了重要转折,即从缺乏现代意识,落后于时代和大众的纯商业产品,转型为具有启蒙精神和现实主义品格的新兴艺术;而话剧在影响电影创作的同时也得... 详细信息

中国的话剧与电影在20世纪30年代发生了前所未有的交集与碰撞。在双向互动中,电影的发展产生了重要转折,即从缺乏现代意识,落后于时代和大众的纯商业产品,转型为具有启蒙精神和现实主义品格的新兴艺术;而话剧在影响电影创作的同时也得到了观念、方法、技巧上的反哺,两种艺术样式从此开始了紧密的同步的发展。

关键词：话剧电影左翼戏剧软硬电影之争观念互动

The Relationship between Thyroid Hormone Levels and Corrected QT Interval and QT Dispersion in Non-Diabetic Hemodialysis Patients

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Open Journal of Nephrology 2014年第1期4卷 13-19页

作者： Heo-Yeong Kim ji Soo Kim Seung Eun Suh Yu Kyung Hyun Kyeong Mi Park Hyung-Jong Kim Department of Internal Medicine Bundang CHA Medical Center CHA University Seongnam South Korea

Background: Cardiovascular disease and sudden cardiac death are common in hemodialysis patients. These cardiac complications are often associated with prolonged QTc interval (QTc) and QTc dispersion (QTcd). Subclinical hypothyroidism (sh) can alter autonomic modulation of heart rate and cause increased inhomogeneity of ventricular recovery time. We aimed to evaluate the relationship between thyroid hormone levels and QTc and QTcd in non-diabetic hemodialysis patients. Methods: We enrolled 29 non-diabetic hemodialysis patients without thyroid disease. After each hemodialysis session, a 12-lead ECG was recorded. Before each hemodialysis session, routine laboratory tests and measurement of thyroid hormone levels were performed. Patients were divided into 2 groups according to QTc (group 1 QTc ms, group 2 QTc ≥ 430 ms). We examined the relationship between QTc or QTcd and thyroid hormone in the respective groups and then compared the results from the 2 groups. Results: The mean age was 54.06 ± 14.72 years and the means of QTc and QTcd were 433.82 ± 22.03 ms, 59.10 ± 28.29 ms, respectively. Homocysteine levels were significant higher in group 2 than group 1 (p 0.05) and QTcd was comparable between groups. In group 1, QTc and QTcd were not significant correlated with Tsh, T3, fT4 and biochemical parameters. In group 2, QTc was significant positively correlated with Tsh (p 0.05) and QTcd was not significant correlated with thyroid hormone levels. Conclusion: The results of this study showed that Tsh is associated with prolonged QTc interval and hyperhomocysteinemia in non-diabetic hemodialysis patients. Moreover, we suggest that sh may be associated with prolonged QTc in non-diabetic hemodialysis patients. However, further studies are required to elucidate the role of the L-thyroxine doses and Tsh target levels in hemodialysis patients.

关键词： Thyroid Hormone Hemodialysis Cardiovascular Disease

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裸眼井和套管井中偶极声场的辐射和接收

中国石油大学学报（自然科学版） 2013年第5期37卷 57-64页

作者：唐晓明曹景记魏周拓中国石油大学地球科学与技术学院 COSL-UPC声学测井联合实验室山东青岛266580

运用弹性波互易原理,指出并验证井中声场辐射和接收存在的互易性,使得井的接收响应可以由井的辐射因子计算得到,并结合辐射声场的远场渐近解,使得远探测声场的计算效率显著提高;模拟结果与三维有限差分数值模拟结果吻合。由于井对声场... 详细信息

运用弹性波互易原理,指出并验证井中声场辐射和接收存在的互易性,使得井的接收响应可以由井的辐射因子计算得到,并结合辐射声场的远场渐近解,使得远探测声场的计算效率显著提高;模拟结果与三维有限差分数值模拟结果吻合。由于井对声场辐射和接收的调制作用,偶极横波远探测测井数据sh型横波的贡献远大于SV型横波的贡献,这一结果为数据的处理和解释提供了理论基础;模拟数据及其成像处理结果还证明了横波远探测技术在套管井中的可行性。所提的理论和方法可以快速模拟偶极横波远探测声场。

关键词：横波反射成像偶极声源井中辐射井中接收弹性波互易原理套管井

Therapeutic effect of cross-shaped moxibustion on the conception vessel and belt vessel for 23 cases of dysmenorrhea due to qi and blood deficiency

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World Journal of Acupuncture-Moxibustion 2020年第2期30卷 138-140页

作者： Xu-hao LI Lin DING Xin MA Xi-qing XUE ji-guo YANG College of Acupuncture and Massage Shandong University of Traditional Chinese MedicineJinan 250355China

Objective:Objective:To observe the therapeutic effect of cross-shaped moxibustion on the conception vessel and belt vessel for dysmenorrhea due to qi and blood ***:Taking shenque(神阙CV8)as the center,up to Zhongwan(中脘CV12),down to Zhongji(中极CV3),and both sides to Daimai(带脉GB26),the above mentioned were treated *** patient’s umbilicus was filled with the herbal powder for moxibustion and the powder was also scattered in a straight line along the treated *** two sheets of mulberry paper were put in a cross way on the herbal powder for moxibustion,and the cross-shaped moxibustion box was put on the *** ginger paste was put into the box to a height of 3 cm and pressed tightly with a tongue depressor,the margin of the ginger paste should be clinged to the moxibustion *** moxa cones were put end to end in the groove on the median of the ginger paste and ignited *** the cones were burnt completely,the new cones were used and *** cones were adopted in succession in each *** treatment was given once a week,and three treatments as one course,the therapeutic effect was evaluated after two courses of ***:There were 16 cases cured,5 cases effective and 2 cases non-effective in *** total effective rate was 91.3%.Conclusion:Coss-shaped moxibustion on the conception vessel and belt vessel has sound effect for dysmenorrhea due to qi and blood deficiency.

关键词： Cross-shaped moxibustion Conception vessel Belt vessel Qi and blood deficiency Dysmenorrhea

Oncogenic potential of IDH1R132C mutant incholangiocarcinoma development in mice

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World Journal of Gastroenterology 2016年第6期22卷 2071-2080页

作者： Ning Ding Li Che Xiao-Lei Li Yan Liu Li-jie jiang Biao Fan Jun-Yan Tao Xin Chen jia-Fu ji Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education) Department of Gastrointestinal SurgeryPeking University Cancer Hospital and InstituteBeijing 100142China Department of Bioengineering and Therapeutic Sciences Liver CenterUniversity of CaliforniaSan FranciscoCA 94143United States Department of Hepatobiliary Surgery Xijing HospitalThe Fourth Military Medical UniversityXi’an 710032Shanxi ProvinceChina School of Pharmacy Hubei University of Chinese MedicineWuhan 430065Hubei ProvinceChina

AIM: To investigate whether IDH1R132 C mutant in combination with loss of p53 and activated Notch signaling promotes intrahepatic cholangiocarcinoma(ICC) ***: We applied hydrodynamic injection and sleeping beauty mediated somatic integration to induce loss of p53(via sh P53), activation of Notch [via intracellular domain of Notch1(NICD)] and/or overexpression of IDH1R132 C mutant together with the sleeping beauty transposase into the mouse liver. Specifically, we co-expressed sh P53 and NICD(sh P53/NICD, n = 4), sh P53 and IDH1R132C(sh P53/IDH1R132 C, n = 3), NICD and IDH1R132C(NICD/IDH1R132 C, n = 4), as well as NICD, sh P53 and IDH1R132C(NICD/sh P53/IDH1R132 C, n = 9) in mice. Mice were monitored for liver tumor development and euthanized at various time points. Liver histology was analyzed by hematoxylin and eosin staining. Molecular features of NICD/sh P53/IDH1R132 C ICC tumor cells were characterized by Myc tag, Flag tag, Ki-67, p-Erk and p-AKT immunohistochemical staining. Desmoplastic reaction in tumor tissues was studied by Picro-Sirius red ***: We found that co-expression of sh P53/NICD, sh P53/IDH1R132 C or NICD/IDH1R132 C did not lead to liver tumor formation. In striking contrast, coexpression of NICD/sh P53/IDH1R132 C resulted in ICC development in mice(P sh P53/IDH1R132 C liver tumors shared multiple histological features of human ICCs, including hyperplasia of irregular glands. Importantly, all tumor cells were positive for the biliary epithelial cell marker cytokeratin 19. Extensive collagen fibers could be visualized in tumor tissues using Sirus red staining, duplicating the desmoplastic reaction observed in human ICC. Tumors were highly proliferative and expressed ectopically injected genes. Toget

关键词： IDH1 mutant Notch pathway Intrahepaticcholangiocarcinoma Mouse liver cancer p53