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Open journal of Obstetrics and Gynecology（妇产科期刊） 2023年第9期

作者： Muela Andy Mbangama Otem Christian Ndesanzim Ambis joëlle Lumaya Nkongolo Freddy Muamba Mubalamata Eugène-patrick Lukusa Nkashama Bienvenu Kazadi Ndombasi Nelda Lemba Banza jésual Lotoy Feruzi Michel Mangala

Rationale of a Cross Sectional Descriptive Study on Knowledge and Practices of Healthcare Providers on Postpartum Hemorrhage Management in Kinshasa, the Democratic Republic of the Congo

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Open journal of Obstetrics and Gynecology（妇产科期刊） 2023年第9期

作者： Muela Andy Mbangama Mubalamata Eugène-patrick Lukusa Ambis joëlle Lumaya Nkongolo Freddy Muamba Banza jésual Lotoy Nkashama Bienvenu Kazadi Ndombasi Nelda Lemba Otem Christian Ndesanzim Feruzi Michel Mangala

Cohort Study on Benefit of Martial Supplementation with EDTA Sodium Iron in Management of Gravidic Anemia at the University Clinics of Kinshasa

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Open journal of Obstetrics and Gynecology（妇产科期刊） 2023年第10期

作者： Andy Muela Mbangama Ndombasi Nelda Lemba Ambis joëlle Lumaya Nkongolo Freddy Muamba Banza jésual Lotoy Mubalamata Eugène-patrick Lukusa Nkashama Bienvenu Kazadi Otem Christian Ndesanzim Feruzi Michel Mangala

Rationale of Longitudinal Cohort Study on Obstetrical Outcomes of Trichomonas vaginalis Infection in Kinshasa, DR Congo

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Open journal of Obstetrics and Gynecology（妇产科期刊） 2023年第9期

作者： Muela Andy Mbangama Banza jésual Lotoy Ambis joëlle Lumaya Nkongolo Freddy Muamba Mubalamata Eugène-patrick Lukusa Nkashama Bienvenu Kazadi Ndombasi Nelda Lemba Otem Christian Ndesanzim Feruzi Michel Mangala

Historic accounts of Mansonella parasitaemias in the South Pacific and their relevance to lymphatic filariasis elimination efforts today

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Asian Pacific journal of Tropical Medicine 2016年第3期9卷 201-207页

作者： j.Lee Crainey Tullio Romao Ribeiro da Silva Sergio Luiz Bessa Luz Ecologia de Doencas Transmissiveis na Amazonia Instituto Leonidas e Maria Deane-Fiocruz Amazonia Rua Terezina

There are two species of filarial parasites with sheathless microfilariae known to commonly cause parasitaemias in humans:Mansonella perstans and Mtinsonella *** most contemporary accounts of the distribution of these parasites,neither is usually considered to occur anywhere in the Eastern ***,Sir patrick Manson,who first described both parasite species,recorded the existence of sheathless sharp-tailed Mansonella ozzardilike parasites occurring in the blood of natives from New Guinea in each and every version of his manual for tropical disease that he wrote before his death in ***'s reports were based on his own identifications and were made from at least two independent blood sample collections that were taken from the *** region Mansonella perstans parasitaemias were also later(in 1923) reported to occur in New Guinea and once before this(in 1905) in *** Mansonella-parasilaernias are generally regarded as benign,they are thought to be of public health importance because they can affect the epidemiological monitoring of other filarial *** this article,we reviewed the historic literature concerning Pacific-origin Mansonella-parasitaemias in an attempt to explain how,despite repeated reports of Pacificregion Mansonella-parasilaemias,by as early as the 1970 s,the WHO had arrived at the presentday view that Wuchereria bancrofti is the only cause of filarial parasitaemias in Papua New *** have also evaluated the evidence supporting the contemporary existence of Pacificarea parasitaemia-causing Mansonella parasites and assessed the relevance such parasites could have for present-day lymphatic filariasis elimination efforts in the region.

关键词： Mansonella ozzardi Mansonella perstans Wuchereria bancrofti Papua New Guinea Lymphatic filariasis Global Programme to Eliminate Lymphatic Filariasis

Plant Biosystems Design Research Roadmap 1.0

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BioDesign Research 2020年第1期2020卷 53-90页

作者： Xiaohan Yang june I.Medford Kasey Markel patrick M.Shih Henrique C.De Paoli Cong T.Trinh Alistair j.McCormick Raphael Ployet Steven G.Hussey Alexander A.Myburg Poul Erik jensen Md Mahmudul Hassan jin Zhang Wellington Muchero Udaya C.Kalluri Hengfu Yin Renying Zhuo Paul E.Abraham jin-Gui Chen David j.Weston Yinong Yang Degao Liu Yi Li jessy Labbe Bing Yang jun Hyung Lee Robert W.Cottingham Stanton Martin Mengzhu Lu Timothy j.Tschaplinski Guoliang Yuan Haiwei Lu Priya Ranjan julie C.Mitchell Stan D.Wullschleger Gerald A.Tuskan Biosciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA The Center for Bioenergy Innovation Oak Ridge National LaboratoryOak RidgeTN 37831USA Department of Biology Colorado State UniversityFort CollinsCO 80523USA Department of Plant Biology University of CaliforniaDavisDavisCAUSA Feedstocks Division Joint BioEnergy InstituteEmeryvilleCAUSA Department of Biodesign Biological Systems and Engineering DivisionLawrence Berkeley National LaboratoryBerkeleyCAUSA Department of Chemical and Biomolecular Engineering University of TennesseeKnoxvilleTN 37996USA SynthSys and Institute of Molecular Plant Sciences School of Biological SciencesUniversity of EdinburghEdinburgh EH93BFUK Department of Biochemistry Genetics and MicrobiologyForestry and Agricultural Biotechnology Institute(FABI)University of PretoriaPretoria 0002South Africa Department of Food Science University of CopenhagenRolighedsvej 26DK-1858FrederiksbergCopenhagenDenmark State Key Laboratory of Subtropical Silviculture School of Forestry and BiotechnologyZhejiang A&F UniversityHangzhouZhejiang 311300China State Key Laboratory of Tree Genetics and Breeding Research Institute of Subtropical ForestryChinese Academy of ForestryHangzhouZhejiang 311400China Department of Plant Pathology and Environmental Microbiology and the Huck Institute of the Life Sciences The Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Genetics Cell Biology and DevelopmentCenter for Precision Plant Genomics and Center for Genome EngineeringUniversity of MinnesotaSaint PaulMN 55108USA Department of Plant Science and Landscape Architecture University of ConnecticutStorrsCT 06269USA Division of Plant Sciences Bond Life Sciences CenterUniversity of MissouriColumbiaMOUSA Donald Danforth Plant Science Center St.LouisMOUSA Environmental Sciences Division Oak Ridge National LaboratoryOak RidgeTN 37831USA

Human life intimately depends on plants for food,biomaterials,health,energy,and a sustainable *** plants have been genetically improved mostly through breeding,along with limited modification via genetic engineering,yet they are still not able to meet the ever-increasing needs,in terms of both quantity and quality,resulting from the rapid increase in world population and expected standards of living.A step change that may address these challenges would be to expand the potential of plants using biosystems design *** represents a shift in plant science research from relatively simple trial-and-error approaches to innovative strategies based on predictive models of biological *** biosystems design seeks to accelerate plant genetic improvement using genome editing and genetic circuit engineering or create novel plant systems through de novo synthesis of plant *** this perspective,we present a comprehensive roadmap of plant biosystems design covering theories,principles,and technical methods,along with potential applications in basic and applied plant biology *** highlight current challenges,future opportunities,and research priorities,along with a framework for international collaboration,towards rapid advancement of this emerging interdisciplinary area of ***,we discuss the importance of social responsibility in utilizing plant biosystems design and suggest strategies for improving public perception,trust,and acceptance.

关键词： Plant utilizing breeding

Light intensity and hydrogel soil amendment differentially affect growth and photosynthesis of successional tree species

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林业研究（英文版） 2023年第1期

作者： Léo Vieira Leonel Fabrício de Oliveira Reis Fábio Afonso Mazzei Moura de Assis Figueiredo Tiago Massi Ferraz Sebasti?o de Oliveira Maia júnior patrick Costa Silva jailma Ribeiro de Andrade

Rationale of Cross-Sectional Descriptive Study on Clinical Effectiveness of Oral Combination Therapy Based on Secnidazole, Azithromycin and Fluconazole in Syndromic Treatment of Abnormal Vaginal Discharge in Kinshasa/DR Congo

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Open journal of Obstetrics and Gynecology（妇产科期刊） 2023年第11期

作者： Muela Andy Mbangama Feruzi Michel Mangala Di-Mosi-Nkoyi Roger Wumba Ambis joelle Lumaya Nkongolo Freddy Muamba Mubalamata Eugene-patrick Lukusa Nkashama Bienvenu Kazadi Ndombasi Nelda Lemba Banza jésual Lotoy Oteme Christian Ndesanzim Mwimba Roger Mbungu

双频双脉冲(ND:YAG)激光机和钬(Ho:YAG)激光机在结石上移和碎石效率方面的体外比较

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国外医学（泌尿系统分册） 2005年第6期25卷 822-824页

作者： Charles G.Marguet jeff C.Sung W.patrick Springhart james O.F.L'esperance Songlin Zhou Per Zhong David M.Albala Glenn M.Preminger 廖国强(译) 袁涛(校) 不详 Duke大学医学中心外科系(综合性肾结石中心泌尿分部)美国北卡罗莱那州达累姆市 Duke大学医学中心机械工程系美国北卡罗莱那州达累姆市 Duke大学医学中心材料学系美国北卡罗莱那州达累姆市上海同济大学附属同济医院泌尿外科

目的为了比较FREDDY激光机、钬激光机和气压弹道碎石机在结石上移和碎石方面的临床效果。方法上移的水下试验装置包括一个水平方向放置的硅树脂导管(直径为1.3cm),和一个固定支架,使激光机的石英光纤和气压弹道碎石机的撞针可以直接接... 详细信息

目的为了比较FREDDY激光机、钬激光机和气压弹道碎石机在结石上移和碎石方面的临床效果。方法上移的水下试验装置包括一个水平方向放置的硅树脂导管(直径为1.3cm),和一个固定支架,使激光机的石英光纤和气压弹道碎石机的撞针可以直接接触石头。将预先称过重的圆柱状Bego石头(BegoUSA公司提供)放于该装置中,分别由FREDDY激光机、钬激光机和气压弹道碎石机进行碎石。FREDDY激光机和钬激光机都采用相近的脉冲能量和频率。为了具有可比性,气压弹道碎石机采用了半硬性撞针和单脉冲,在100、200、300kPa各档压力设置下分别对石头进行30次撞击。“平均后退距离”定义为直接测得的石头最终静止点到原点的距离(每档设置都记录测试结果)。另外,两台激光机分别对称过重的石头(熟石膏成分)进行碎石,以比较碎石效果。石头固定于一个水下装置(由一个倒置的硅注射器和一个固定装置组成)中。激光光纤(钬激光和FREDDY激光的光纤内径分别是365μm和280μm)穿过注射器的针头出来抵住石头。总共24颗石头被分成4组,每组6颗,其中两组用FREDDY激光分别以300j和400j的总能量碎石,另两组用钬激光分别以300j和480j的总能量碎石。“碎石率”定义为石头重量缺失的百分比。结果FREDDY激光机在能量设置为160mj时,分别以5、10和15Hz的频率碎石,石头的平均后退距离为7.6、8.1和6.8cm;钬激光机在能量设置为0.8j时,分别以5和10Hz的频率碎石,石头的平均后退距离为3.3和4.9cm;气压弹道碎石机在压力设置分别为100、200、300kPa时,石头的平均后退距离为8.5、9.9和13.8cm。一般来说,FREDDY激光机造成的石头后退距离都要少于气压弹道碎石机,尽管这只有在后者处于最高压力设置时才有统计意义(pj和400j的总能量碎石时,石头重量的缺失率分别为44.9%和86.8%,都有统计意义(pj和480j的总能量碎石时,石头重量的缺失率分别为3.3%和7.1%,都有统计意义(p<0.05)。结论FREDDY激光机造成的石头后退距离要明显大于钬激光机(在低频设置时),但又明显少于气压弹道碎石机(在所有设置时)。因此我们建议,在进行腔内输尿管镜碎石术,或在输尿管肾盂连接处进行腔内激光碎石术时,于接近结石处使用阻断装置,如StoneCone-石椎(BostonScientific公司提供)。FREDDY激光机在体外碎石的效率要明显好于钬激光机,对于结石病人来说,前者是个更优、更经济的选择。

关键词：尿路结石碎石术激光钕钬

If this is true, what does it imply? How end-user antibody validation facilitates insights into biology and disease

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Asian journal of Urology 2019年第1期6卷 10-25页

作者： Karen S.Sfanos Srinivasan Yegnasubramanian William G.Nelson Tamara L.Lotan Ibrahim Kulac jessica L.Hicks Qizhi Zheng Charles j.Bieberich Michael C.Haffner Angelo M.De Marzo Department of Pathology Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Oncology The Johns Hopkins University School of MedicineBaltimoreMDUSA Department of Pathology Koc Universitesi Tip FakultesiIstanbulTurkey Department of Biological Sciences University of Maryland Baltimore CountyBaltimoreMDUSA

Antibodies are employed ubiquitously in biomedical sciences,including for diagnostics and *** of the most important uses is for immunohistochemical(IHC)staining,a process that has been improving and evolving over *** is useful when properly employed,yet misuse of the method is widespread and contributes to the“reproducibility crisis”in *** report some of the common problems encountered with IHC assays,and direct readers to a wealth of literature documenting and providing some solutions to this *** also describe a series of vignettes that include our approach to analytical validation of antibodies and IHC assays that have facilitated a number of biological insights into prostate cancer and the refutation of a controversial association of a viral etiology in *** postulate that a great deal of the problem with lack of accuracy in IHC assays stems from the lack of awareness by researchers for the critical necessity for end-users to validate IHC antibodies and assays in their laboratories,regardless of manufacturer claims or past *** suggest that one reason for the pervasive lack of end-user validation for research antibodies is that researchers fail to realize that there are two general classes of antibodies employed in ***,there are antibodies that are“clinical grade”reagents used by pathologists to help render diagnoses that influence patient *** diagnostic antibodies,which tend to be highly validated prior to clinical implementation,are in the vast minority(e.g.3800000),which are often not extensively validated prior to *** increased awareness of the problem,both the United States,National Institutes of Health and some journals are requiring investigators to provide evidence of specificity of their antibody-based assays.

关键词： Prostate cancer Antibodies Immunohistochemistry