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APPLICAtION OF HIGH PRESSURE LIQUID CHROMAtOGRAPHY AND MICROSEQUENCING MEtHODOLOGY IN tHE StRUCtURAL ANALYSIS OF HUMAN HEMOGLObIN VARIANtS

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Science China Chemistry 1983年第8期26卷 836-849页

作者：曾溢滔,黄淑帧,A.REYNOLDS,H.LAM,b.b.WEbbER,j.b.WILSON,t.H.j.HUISMAN Laboratory of Medical Genetics Shanghai Children's Hospital Shanghai People's Republic of China Laboratory of Medical Genetics Shanghai Children's Hospital Shanghai People's Republic of China Laboratory of Protein Chemistry Comprehensive Sickle Cell Center Department of Cell and Molecular biology Medical Collge of Georgia Augusta Ga 30912 U. S. A. Laboratory of Protein Chemistry Comprehensive Sickle Cell Center Department of Cell and Molecular biology Medical Collge of Georgia Augusta Ga 30912 U. S. A. Laboratory of Protein Chemistry Comprehensive Sickle Cell Center Department of Cell and Molecular biology Medical Collge of Georgia Augusta Ga 30912 U. S. A. Laboratory of Protein Chemistry Comprehensive Sickle Cell Center Department of Cell and Molecular biology Medical Collge of Georgia Augusta Ga 30912 U. S. A. Laboratory of Protein Chemistry Comprehensive Sickle Cell Center Department of Cell and Molecular biology Medical Collge of Georgia Augusta Ga 30912 U. S. A.

this paper reports the results of the analyses of 17 different Hb variants which were observed in 37 families. High pressure liquid chromatography (HPLC) was used to separate tryptic and thermolytic peptides of globin chains. the double coupling microsequencing technique can successfully be used for sequencing small or larger peptides.

关键词： APPLICAtION OF HIGH PRESSURE LIQUID CHROMAtOGRAPHY AND MICROSEQUENCING MEtHODOLOGY IN tHE StRUCtURAL ANALYSIS OF HUMAN HEMOGLObIN VARIANtS HPLC

NECAb family of neuronal calcium-binding proteins in health and disease

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Neural Regeneration Research 2025年第5期20卷 1236-1243页

作者： Diones bueno Michael K.E.Schafer Sudena Wang Michael j.Schmeisser Axel Methner Institute for Molecular Medicine University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Department of Anesthesiology University Medical Center of the Johannes Gutenberg-UniversityMainzGermany Institute of Anatomy University Medical Center of the Johannes Gutenberg-UniversityMainzGermany

the N-terminal EF-hand calcium-binding proteins 1–3(NECAb1–3) constitute a family of predominantly neuronal proteins characterized by the presence of at least one EF-hand calcium-binding domain and a functionally less well characterized C-terminal antibiotic biosynthesis monooxygenase domain. All three family members were initially discovered due to their interactions with other proteins. NECAb1 associates with synaptotagmin-1, a critical neuronal protein involved in membrane trafficking and synaptic vesicle exocytosis. NECAb2 interacts with predominantly striatal G-protein-coupled receptors, while NECAb3 partners with amyloid-β A4 precursor protein-binding family A members 2 and 3, key regulators of amyloid-β production. this demonstrates the capacity of the family for interactions with various classes of proteins. NECAb proteins exhibit distinct subcellular localizations: NECAb1 is found in the nucleus and cytosol, NECAb2 resides in endosomes and the plasma membrane, and NECAb3 is present in the endoplasmic reticulum and Golgi apparatus. the antibiotic biosynthesis monooxygenase domain, an evolutionarily ancient component, is akin to atypical heme oxygenases in prokaryotes but is not wellcharacterized in vertebrates. Prokaryotic antibiotic biosynthesis monooxygenase domains typically form dimers, suggesting that calcium-mediated conformational changes in NECAb proteins may induce antibiotic biosynthesis monooxygenase domain dimerization, potentially activating some enzymatic properties. However, the substrate for this enzymatic activity remains uncertain. Alternatively, calcium-mediated conformational changes might influence protein interactions or the subcellular localization of NECAb proteins by controlling the availability of protein–protein interaction domains situated between the EF hands and the antibiotic biosynthesis monooxygenase domain. this review summarizes what is known about genomic organization, tissue expression, intracellular localization, interac

关键词： calcium EF hand mitochondria nervous system signal transduction

A comprehensive study on the processing of Co:ZnO nanostructured ceramics:Defect chemistry engineering and grain growth kinetics

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journal of Materials Science & technology 2023年第7期138卷 221-232页

作者： R.t.da Silva j.M.Morbec G.Rahman H.b.de Carvalho Universidade Federal de Ouro Preto-UFOP Ouro PretoMG 35400-000Brazil School of Chemical and Physical Sciences Keele UniversityKeele ST55BGUnited Kingdom Department of Physics Quaid-i-Azam UniversityIslamabad 45320Pakistan Universidade Federal de Alfenas-UNIFAL Alfenas 37130-000Brazi

In this report,we present a systematic study on the preparation of Co:ZnO ceramics via a standard solid-state route from different Co precursors(Co_(3)O_(4),CoO,and metallic Co)and atmospheres(O_(2)and Ar).Particular emphasis is given to defect chemistry engineering and the sintering growth ***-principles calculations based on density functional theory are employed to determine the formation en-ergy of the main point defects in ZnO and Co:ZnO *** on the theoretical results,a set of chemical reactions is *** microstructural characterization is also performed to determine the degree of Co incorporation into the ZnO *** samples prepared in Ar atmosphere and metal-lic Co present the highest Co solubility limit(lower apparent Co incorporation activation energy)due to the incongruent ZnO *** of the parameters of the sintering growth kinetics reveals that Co_(3)O_(4)is the best sintering additive to achieve larger grain sizes,and possible higher densi-ties,in both sintering atmospheres,while metallic Co is the best to achieve the smallest grain size with higher Co concentration and homogeneous spatial distribution for a subsequent reduction of *** results show that the sintering in O_(2)effectively promotes zinc vacancies in the ZnO structure,while the sintering in Ar promotes zinc interstitial *** findings contribute to understanding the preparation of Co-doped ZnO ceramics and the sintering growth kinetics,which may improve the state of the art in processing the material at both bulk and nanometric scales.

关键词： Multifunctionality Zinc oxide Defect engineering Growth kinetics

Power management unit chip design for automobile active-matrix organic light-emitting diode display module

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journal of Central South University 2009年第4期16卷 621-628页

作者： KIM j H PARK j H KIM j H CAO t V LEE t Y bAN H j YANG K KIM H G HA P b KIM Y H Department of Electronic Engineering Changwon National University 9 Sarim-Dong Changwon 641-773 Korea Denso PS Electronics Corporation 47 Seongsan-Dong Changwon 641-315 Korea

A power management unit （PMU） chip supplying dual panel supply voltage, which has a low electro-magnetic interference （EMI） characteristic and is favorable for miniaturization, is designed. A two-phase charge pump circuit using external pumping capacitor increases its pumping current and works out the charge-loss problem by using bulk-potential biasing circuit. A low-power start-up circuit is also proposed to reduce the power consumption of the band-gap reference voltage generator. And the ring oscillator used in the ELVSS power circuit is designed with logic devices by supplying the logic power supply to reduce the layout area. the PMU chip is designed with MagnaChip＇s 0.25 μ high-voltage process. the driving currents of ELVDD and ELVSS are more than 50 mA when a SPICE simulation is done.

关键词： DC-DC converter AMOLED charge pumping power management unit (PMU) dual panel supply voltage

NONLINEAR FREE SURFACE ACtION WItH AN ARRAY OF VERtICAL CYLINDERS

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Acta Mechanica Sinica 2004年第3期20卷 247-262页

作者： HUANG j.b. Department of Engineering Science University of OxfordUK

Nonlinear diffraction of regular waves by an array of bottom-seated circular cylindersis investigated in frequency domain,based on a Stokes expansion approach.A complete semi-analyticalsolution is developed which allows an efficient evaluation of the second-order potentials in the entirefluid domain,and the wave forces on the *** are derived for the second-orderpotential in the vicinity of individual *** expressions have a simple form,thus providingan effective means for investigating the wave enhancement due to nonlinear interactions with *** on the present method,the wave run-up and free-surface elevations around an arrayof two,three and four cylinders are investigated numerically.

关键词： nonlinear wave diffraction water waves frequency analysis semi-analytical method

A corrected particle method with high-order taylor expansion for solving the viscoelastic fluid flow

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Acta Mechanica Sinica 2017年第1期33卷 20-39页

作者： t. jiang j. L. Ren W. G. Lu b. Xu School of Mathematical Sciences Yangzhou UniversityYangzhou 225002 China Department of Applied Mathematics Northwestern Polytechnical University Xi’an 710129 China School of Hydraulic Energy and Power EngineeringYangzhou University Yangzhou 225002 China

In this paper, a corrected particle method based on the smoothed particle hydrodynamics (SPH) method with high-order taylor expansion (CSPH-Ht) for solving the viscoelastic flow is proposed and investigated. the validity and merits of the CSPH-Ht method are first tested by solving the nonlinear high order Kuramoto-Sivishinsky equation and simulating the drop stretching, respectively. then the flow behaviors behind two stationary tangential cylinders of polymer melt, which have been received little attention, are investigated by the CSPH-Ht method. Finally, the CSPH-Ht method is extended to the simulation of the filling process of the viscoelastic fluid. the numerical results show that the CSPH-Ht method possesses higher accuracy and stability than other corrected SPH methods and is more reliable than other corrected SPH methods.

关键词： Smoothed particle hydrodynamics High-order taylor expansion Viscoelastic fluid Extended pom-pom model

StUDY ON bIOLEACHING OF PRIMARY CHALCOPYRItE ORE WItH tHERMOACIDOPHLIC ARCHAE

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Acta Metallurgica Sinica(English Letters) 2006年第5期19卷 341-346页

作者： P. Zou W.b. Zhang t. Lei j.K. Wang Kunming University of Science and technology Kunming 650093 China technology Center Yunnan Metallurgy Group Co. Ltd. Kunming 650031China

A high temperature-tolerating thermoacidophilic archae （tA） was isolated from water samples collected from a hot sulfur-containing spring in the Yunnan Province, China, and was used in bioleaching experiments of a low-grade chalcopyrite ore. the tA grow at temperatures ranging from 40 to 80℃, with 65℃ being the optimum temperature, and at pH values of l.5 to 4.0, with an optimum pH value of 2.0. the bioleaching experiments of the chalcvpyrite ore were conducted in both laboratory batch bioreactors and leaching columns. the results obtained from the bioreactor experiments showed that the tA bioleaching rate of copper reached 97% for a 12-day leaching period, while the bioleaching rate was 32.43% for thiobacillus ferrooxidans （tf） leaching for the same leaching time. In the case of column leaching, tests of a two-phase leaching （196 days）, that is, a two-month （56 days） tf leaching in the first phase, followed by a 140-day tA leaching in the second phase were performed. the average leaching rate of copper achieved for the 140-day tA leaching was 195mg/（L.d）, while for the control experiments, it was as low as 78mg/（L .d） for the tf leaching, indicating that the tA possesses a more powerful oxidizing ability to the chalcopyrite than tf therefore, it is suggested that the two-phase leaching process be applied to .for the heap leaching operations, whereas, the tA can be used in the second phase when the temperature inside the heap has increased, and the primary copper sulfide minerals have already been partially oxidized with tf beforehand in the first phase.

关键词： chalcopyrite biological oxidation thermoacidophilic archae mesophile batch stirred bioleaching column bioleaching

Analysis of the factors affecting LiDAR DtM accuracy in a steep shrub area

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International journal of Digital Earth 2011年第6期4卷 521-538页

作者： j.Estornell L.A.Ruiz b.Vela´zquez-Martı´ t.Hermosilla Departamento de Ingenierı´a Cartogra´fica Geodesia y Fotogrametrı´aUniversidad Polite´cnica de ValenciaCamino de Vera s/nValencia 46022Spain Departamento de Ingenierı´a Rural y Agroalimentaria Universidad Polite´cnica de ValenciaValenciaSpain

the creation of a quality Digital terrain Model(DtM)is essential for representing and analyzing the Earth in a digital *** continuous improvements in the acquisition and the potential of airborne Light Detection and Ranging(LiDAR)data are increasing the range of applications of this technique to the study of the Earth *** aim of this study was to determine the optimal parameters for calculating a DtM by using an iterative algorithm to select minimum elevations from LiDAR data in a steep mountain area with shrub *** parameters were:input data type,analysis window size,and height *** effects of slope,point density,and vegetation on DtM accuracy were also *** results showed that the lowest root mean square error(RMSE)was obtained with an analysis window size of 10 m,5 m,and 2.5 m,rasterized data as input data,and height thresholds equal to or greater than 1.5 *** parameters showed a RMSE of 0.19 *** terrain slope varied from 010%to 5060%,the RMSE increased by 0.11 *** RMSE decreased by 0.06 m when point density was increased from 4 to 8 points/m2,and increased by 0.05 m in dense vegetation areas.

关键词： LiDAR digital earth Digital terrain Model(DtM) filtering 3-D representation

Exact solutions to drift-flux multiphase flow models through Lie group symmetry analysis

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Applied Mathematics and Mechanics(English Edition) 2015年第8期36卷 1105-1112页

作者： b.bIRA t.R.SEKHAR Department of Mathematics National Institute of Science and Technology Department of Mathematics Indian Institute of Technology Kharagpur

In the present paper, Lie group symmetry method is used to obtain some exact solutions for a hyperbolic system of partial differential equations （PDEs）, which governs an isothermal no-slip drift-flux model for multiphase flow problem. those sym- metries are used for the governing system of equations to obtain infinitesimal transforma- tions, which consequently reduces the governing system of PDEs to a system of ODEs. Further, the solutions of the system of ODEs which in turn produces some exact solutions for the PDEs are presented. Finally, the evolutionary behavior of weak discontinuity is discussed.

关键词： multiphase flow drift-flux models Lie group analysis exact solution weakdiscontinuity