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superior antibody and membrane protein‑specific T‑cell responses to CoronaVac by intradermal versus intramuscular routes in adolescents

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World journal of Pediatrics 2024年第4期20卷 353-370页

作者： jaime s.Rosa Duque samuel M.s.Cheng Carolyn A.Cohen Daniel Leung Xiwei Wang Xiaofeng Mu Yuet Chung Tsun Ming Lau Manni Wang Wenyue Zhang Yanmei Zhang Howard H.W.Wong Leo C.H.Tsang sara Chaothai Tsz Chun Kwan john K.C.Li Karl C.K.Chan Leo L.H.Luk jenson C.H.Ho Wing Yan Li Amos M.T.Lee jennifer H.Y.Lam sau Man Chan Wilfred H.s.Wong Issan Y.s.Tam Masashi Mori sophie A.Valkenburg malik peiris Wenwei Tu Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina school of Public Health The University of Hong KongHong KongChina HKU‑Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Microbiology and Immunology Peter Doherty Institute for Infectionand ImmunityUniversity of MelbourneMelbourneVICAustralia Center for Immunology and Infection C2i Hong KongChina

Background Optimising the immunogenicity of COVID-19 vaccines to improve their protection against disease is *** dosing by intradermal(ID)administration has been shown to be equally immunogenic as intramuscular(IM)administration for several vaccines,but the immunogenicity of ID inactivated whole severe acute respiratory syndrome coronavirus 2(sARs-CoV-2)at the full dose is *** study(NCT04800133)investigated the superiority of antibody and T-cell responses of full-dose CoronaVac by ID over IM administration in *** Participants aged 11–17 years received two doses of IM or ID vaccine,followed by the 3rd dose 13–42 days *** and cellular immunogenicity outcomes were measured post-dose 2(IM-CC versus ID-CC)and post-dose 3(IMCCC versus ID-CCC).Doses 2 and 3 were administered to 173 and 104 adolescents,*** spike protein(s)immunoglobulin G(IgG),s-receptor-binding domain(RBD)IgG,s IgG Fcγreceptor IIIa(FcγRIIIa)-binding,sNM[sum of individual(s),nucleocapsid protein(N),and membrane protein(M)peptide pool]-specific interleukin-2(IL-2)+CD4^(+),sNM-specific IL-2^(+)CD8^(+),s-specific IL-2^(+)CD8^(+),N-specific IL-2^(+)CD4^(+),N-specific IL-2^(+)CD8^(+)and M-specific IL-2^(+)CD4^(+)responses fulfilled the superior and non-inferior criteria for ID-CC compared to IM-CC,whereas IgG avidity was *** ID-CCC,s-RBD IgG,surrogate virus neutralisation test,90%plaque reduction neutralisation titre(PRNT90),PRNT50,s IgG avidity,s IgG FcγRIIIa-binding,M-specific IL-2^(+)CD4^(+),interferon-γ+CD8^(+)and IL-2^(+)CD8^(+)responses were superior and non-inferior to *** estimated vaccine efficacies were 49%,52%,66%and 79%for IM-CC,ID-CC,IM-CCC and ID-CCC,*** ID groups reported more local,mild adverse *** This is the first study to demonstrate superior antibody and M-specific T-cell responses by ID inactivated sARsCoV-2 vaccination and serves as the basis for future research to improve the

关键词： CoronaVac Coronavirus disease 2019 Immunity Intradermal Vaccine

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感染人类的禽流感病毒A(H5N1)研究进展

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中华肺部疾病杂志（电子版） 2009年第1期2卷 47-56页

作者： Abdel-Nasser Abdel-Ghafar Tawee Chotpitayasunondh Zhancheng Gao Frederick G. Hayden Nguyen Duc Hien Menno D. de jong Azim Naghdaliyev j.s. malik peiris Nahoko shindo santoso soeroso Timothy M. Uyeki 钟晓琴（译）王关嵩（校） the Ministry of Health and Population Cairo the Queen sirikit Na-tional Institute of Child Health Bangkok Thailand the Peking University People＇s Hospital Beijing the Global Influenza Program World Health Organization Geneva the University of Virginia Charlottesville the National Institute for Infectious and Tropical Diseases Hanoi the Oxford University Clinical Research Unit Hospital for Tropical Diseases Ho Chi Minh City Vietnam the Abulfaz Karayev Children＇sHospital No. 2 Baku Azerbaijan the University of Hong Kong Hong Kong the National Infectious Diseases Hospital Jakarta Indonesia the Centers for Disease Control and Prevention Atlanta 不详

禽流感病毒A(H5N1)[avian influenza(H5N1)viruses]之前一直存在于鸟类,但却能导致人类疾病,并且具有高致死性和广泛流行的威胁.本文在综合了第二届世界卫生组织(World Health Organization,WHO)感染人类禽流感病毒A(H5N1)临床诊断咨询... 详细信息

禽流感病毒A(H5N1)[avian influenza(H5N1)viruses]之前一直存在于鸟类,但却能导致人类疾病,并且具有高致死性和广泛流行的威胁.本文在综合了第二届世界卫生组织(World Health Organization,WHO)感染人类禽流感病毒A(H5N1)临床诊断咨询会议公布的研究信息[2]基础上,对2005年的报告[1]进行了更新.

关键词：人类禽流感病毒 A(H5N1) 感染人类世界卫生组织人类疾病临床诊断致死性

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Conservation of T cell epitopes between seasonal influenza viruses and the novel influenza A H7N9 virus

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Virologica sinica 2014年第3期29卷 170-175页

作者： Huawei Mao Hui-Ling Yen Yinping Liu Yu-Lung Lau j.s.malik peiris Wenwei Tu Department of Paediatrics The University of Hong Kong Shenzhen Hospital Department of Paediatrics & Adolescent Medicine The University of Hong Kong Centre of Influenza Research School of Public Health The University of Hong Kong

A novel avian influenza A(H7N9) virus recently emerged in the Yangtze River delta and caused diseases, often severe, in over 130 people. This H7N9 virus appeared to infect humans with greater ease than previous avian influenza virus subtypes such as H5N1 and H9N2. While there are other potential explanations for this large number of human infections with an avian influenza virus, we investigated whether a lack of conserved T-cell epitopes between endemic H1N1 and H3N2 influenza viruses and the novel H7N9 virus contributes to this observation. Here we demonstrate that a number of T cell epitopes are conserved between endemic H1N1 and H3N2 viruses and H7N9 virus. Most of these conserved epitopes are from viral internal proteins. The extent of conservation between endemic human seasonal influenza and avian influenza H7N9 was comparable to that with the highly pathogenic avian influenza H5N1. Thus, the ease of inter-species transmission of H7N9 viruses(compared with avian H5N1 viruses) cannot be attributed to the lack of conservation of such T cell epitopes. On the contrary, our findings predict significant T-cell based cross-reactions in the human population to the novel H7N9 virus. Our findings also have implications for H7N9 virus vaccine design.

关键词：禽流感病毒 T细胞表位季节性保护 H5N1病毒高致病性禽流感人类感染甲型

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A novel luciferase immunosorbent assay performs better than a commercial enzyme-linked immunosorbent assay to detect MERs-CoV specific IgG in humans and animals

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Biosafety and Health 2019年第3期1卷 134-143页

作者： Wenling Wang Tianyu Wang Yao Deng Peihua Niu Ruhan A jincun Zhao malik peiris shixing Tang Wenjie Tan MHC Key Laboratory of Biosafety National Institute for Viral Disease Control and PreventionChina CDCBeijing 102206China Guangdong Provincial Key Laboratory of Tropical Disease Research Department of EpidemiologySchool of Public HealthSouthern Medical UniversityGuangzhou 510515China state Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory DiseaseThe first Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510182China school of Public Health Li Ka Shing Faculty of MedicineThe University of Hong KongPokfulamHong Kong Special Administrative Region Center for Biosafety Mega-science Chinese Academy of SciencesBeijing 100101China

The Middle East respiratory syndrome(MERs)is a lethal zoonosis caused by MERs coronavirus(MERs-CoV)and poses a significant threat to public health ***,a rapid,sensitive,and specific serologic test for detecting anti-MERs-CoV antibodies in both humans and animals is urgently needed for the successful management of this ***,we evaluated various novel luciferase immunosorbent assays(LIsA)based on nucleocapsid protein(NP)as well as fragments derived from spike protein(s)including subunit 1(s1),N terminal domain(NTD),receptorbinding domain(RBD)and subunit 2(s2)of s for the detection of MERs-CoV-specific *** proteins,including nanoluciferase(NLuc)and various fragments derived from the NP or s protein of MERs-CoV,were expressed in human embryonic kidney 293 T *** that detected anti-MERs-CoV IgG were further developed using cell lysates expressing various fusion *** of human or animal samples infected with MERs-CoV were used to analyze the sensitivity and specificity of various LIsAs in reference to a MERs-CoV RT-PCR,commercial s1-based ELIsA,and pseudovirus particle neutralization test(ppNT).Our results showed that the s1-,RBD-,and NP-LIsAs were more sensitive than the NTD-and s2-LIsAs for the detection of anti-MERs-CoV ***,the s1-,RBD-,and NP-LIsAs were more sensitive(by at least 16-fold)than the commercially available ***,the s1-,RBD-,and NPLIsA specifically recognized anti-MERs-CoV IgG and did not cross-react with samples derived from other human CoV(OC43,229E,HKU1,NL63)-infected *** importantly,these LIsAs proved their applicability and reliability for detecting anti-MERs-CoV IgG in samples from camels,monkeys,and mice,among which the RBD-LIsA exhibited excellent *** results of this study suggest that the novel MERs-CoV RBD-and s1-LIsAs are highly effective platforms for the rapid and sensitive detection of anti-MERs-CoV IgG in human and animal *** assays have

关键词： Luciferase immunosorbent assay(LIsA) MERs-CoV serological IgG detection samples of humans and animals

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In vitro and in vivo anticancer potential and molecular targets of the new colchicine analog ⅢM-067

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journal of Integrative Medicine 2023年第1期21卷 62-76页

作者： sumera malik Mubashir j.Mintoo Chilakala Nagarjuna Reddy Raj.s. Kumar Pankul Kotwal sandip BBharate Utpal Nandi Dilip M.Mondhe sanket K.shukla Pharmacology Division Council of Scientific and Industrial Research(CSIR)-Indian Institute of Integrative MedicineJammu 180001Jammu&KashmirIndia Academy of scientific&Innovative Research(AcsIR) Ghaziabad 201002Uttar PradeshIndia Medicinal Chemistry Division CSIR-Indian Institute of Integrative MedicineJammu 180001Jammu&KashmirIndia Pharmacokinetics-Pharmacodynamics(PK-PD) Toxicology DivisionCSIR-Indian Institute of Integrative MedicineJammu 180001Jammu&KashmirIndia

Objective: The current study evaluated various new colchicine analogs for their anticancer activity and to study the primary mechanism of apoptosis and in vivo antitumor activity of the analogs with selective anticancer properties and minimal toxicity to normal ***: sulforhodamine B(sRB) assay was used to screen various colchicine analogs for their in vitro cytotoxicity. The effect of N-[(7s)-1,2,3-trimethoxy-9-oxo-10-(pyrrolidine-1-yl)5,6,7,9-tetrahydrobenzo[a] heptalene-7-yl] acetamide(ⅢM-067) on clonogenicity, apoptotic induction, and invasiveness of A549 cells was determined using a clonogenic assay, scratch assay, and staining with 4’,6-diamidino-2-phenylindole(DAPI) and annexin V/propidium iodide. Mitochondrial membrane potential(MMP) and reactive oxygen species(ROs) levels were observed using fluorescence microscopy. Western blot analysis was used to quantify expression of proteins involved in apoptosis, cell cycle, and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)/mammalian target of rapamycin(mTOR) signaling. Pharmacokinetic and in vivo efficacy studies against Ehrlich ascites carcinoma(EAC) and Ehrlich solid tumor models were conducted using swiss albino ***: ⅢM-067 showed potent cytotoxicity and better selectivity than all other colchicine analogs screened in this study. The selective activity of ⅢM-067 toward A549 cells was higher among other cancer cell lines,with a selectivity index(sI) value of 2.28. ⅢM-067 demonstrated concentration-and time-dependent cytotoxicity against A549 cells with half-maximal inhibitory concentration values of 0.207, 0.150 and0.106 μmol/L at 24, 48 and 72 h, respectively. It also had reduced toxicity to normal cells(sI > 1) than the parent compound colchicine(sI = 1). ⅢM-067 reduced the clonogenic ability of A549 cells in a dose-dependent manner. ⅢM-067 enhanced ROs production from 24.6% at 0.05 μmol/L to 82.1% at 0.4 μmol/L and substantially decreased the MMP(100% in control to 5.6% at 0.4 μmol

关键词： Colchicine 1IIM-067 Apoptosis Antineoplastic agents Carcinoma Ehrlich tumor A549 cells

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Antibody and T cell responses against wild-type and Omicron sARs-CoV-2 after third-dose BNT162b2 in adolescents

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signal Transduction and Targeted Therapy 2023年第1期8卷 266-275页

作者： Xiaofeng Mu Carolyn A.Cohen Daniel Leung jaime s.Rosa Duque samuel M.s.Cheng Yuet Chung Howard H.W.Wong Amos M.T.Lee Wing Yan Li Issan Y.s.Tam jennifer H.Y.Lam Derek H.L.Lee sau Man Chan Leo C.H.Tsang Karl C.K.Chan john K.C.Li Leo L.H.Luk sara Chaothai Kelvin K.H.Kwan Nym Coco Chu Masashi Mori Trushar jeevan Ahmed Kandeil Richard jWebby Wenwei Tu sophie A.Valkenburg malik peiris Yu Lung Lau Department of Paediatrics and Adolescent Medicine The University of Hong KongHong KongChina HKU-Pasteur Research Pole School of Public HealthThe University of Hong KongHong KongChina school of Public Health The University of Hong KongHong KongChina Research Institute for Bioresources and Biotechnology Ishikawa Prefectural UniversityNonoichiJapan Department of Infectious Diseases St Jude Children’s Research HospitalMemphisTNUSA Department of Microbiology and Immunology Peter Doherty Institute for Infection and ImmunityUniversity of MelbourneMelbourneVICAustralia Centre for Immunology&Infection C2i Hong KongChina

The high effectiveness of the third dose of BNT162b2 in healthy adolescents against Omicron BA.1 has been reported in some studies,but immune responses conferring this protection are not yet *** this analysis,our study(NCT04800133)aims to evaluate the humoral and cellular responses against wild-type and Omicron(BA.1,BA.2 and/or BA.5)sARs-CoV-2 before and after a third dose of BNT162b2 in healthy *** 5 months after 2 doses,s IgG,s IgG Fc receptor-binding,and neutralising antibody responses waned significantly,yet neutralising antibodies remained detectable in all tested adolescents and s IgG avidity increased from 1 month after 2 *** antibody responses and s-specific IFN-γ^(+)and IL-2^(+)CD8^(+)T cell responses were significantly boosted in healthy adolescents after a homologous third dose of *** to adults,humoral responses for the third dose were non-inferior or superior in *** s-specific IFN-γ^(+)and IL-2^(+)CD4^(+)and CD8^(+)T cell responses in adolescents and adults were comparable or ***,after 3 doses,adolescents had preserved s IgG,s IgG avidity,s IgG FcγRIIIa-binding,against Omicron BA.2,as well as preserved cellular responses against BA.1 s and moderate neutralisation levels against BA.1,BA.2 and *** from 100 and 96%of adolescents tested at 1 and 5 months after two doses could also neutralise *** study found high antibody and T cell responses,including potent cross-variant reactivity,after three doses of BNT162b2 vaccine in adolescents in its current formulation,suggesting that current vaccines can be protective against symptomatic Omicron disease.

关键词： doses neutral protective

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Densities and excess molar volumes of mixtures containing diesel,biodiesel and alkanols at temperatures from 288.15 to 313.15 K

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Chinese journal of Chemical Engineering 2021年第6期34卷 198-207页

作者： sunita malik Poonam jangra Darolia s.K.Garg V.K.sharma Department of Chemistry Maharshi Dayanand UniversityRohtakHaryanaIndia j.C.Bose University of science and Technology YMCAFaridabadHaryanaIndia

The present work is focusing on the synthesization and physico-chemical properties of jatropha curcas biodiesel with diesel and *** densities of binary diesel(2)+1-alkanols(C_(3) or C_(4))(3)and ternary jatropha curcas biodiesel(1)+diesel(2)+1-alkanols(C_(3)or C_(4))(3)blends have been reported over full range of composition at temperatures within range 288.15 to 313.15 *** densities of jatropha curcas biodiesel(1)+diesel or 1-alkanols(C_(3) or C_(4))(2)blends have been measured at 313.15 *** molar volumes,V^E,V^E_(123)of binary and ternary blends were calculated from the measured data and the derived properties were correlated to composition using Redlich-Kister equation.A reasonable agreement was found between the measured and estimated ***,densities and excess molar volumes data were reasoned to discuss molecular interactions taking into consideration effect of composition and temperature.

关键词： jatropha curcas biodiesel Diesel Alcohols Blends Density Excess molar volumes

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Effects of acut and chronic doses of methoxy acetic acid on hamster sperm fertilising ability

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Asian journal of Andrology 2001年第3期3卷 209-216页

作者： L.D.C.peiris H.D.M.Moore Dept. of Zoology University of Colombo Colombo 03 Sri LankaDept. of Molecular Biology and Biotechnology University of Sheffield Sheffield S10 2TN UK

Aim: To evaluate the effects of acute and chronic doses of methoxy acetic acid (MAA) on in vitro fertilisation byhamster sperm and to correlate the data with the testicular damage. Methods: Adult male hamsters were gavagedwith 3 single doses (0, 80, 160 and 650 mg/kg) and 3 chronic doses (0, 8, 32 and 64 mg/kg daily for 5 weeks) ofMAA in distilled water. After treatment hamsters were killed at weekly intervals and spermatozoa recovered from thedistal cauda epididymides were used to assess the fertilising capacity in vitro. The testes were processed for histologicalexamination. Results; Acute doses showed a significant reduction in sperm fertilising ability from week 3 and 4 af-ter treatment and with the chronic doses, the effects were more extensive and persistent. The results were in correpon-dence with the testicular damages observed. Conclusion: It is evident that both acute and chronic doses of MAAcan impair the sperm function by damaging one or more cell populations in the testis.(Asian j Androl 2001 sep; 3: 209- 216)

关键词： methoxy acetic acid fertilisation in vitro testis spermatozoa fertility

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Impediments of Cognitive system Engineering in Machine-Human Modeling

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Computers, Materials & Continua 2023年第3期74卷 6689-6701页

作者： Fayaz Ahmad Fayaz Arun malik Isha Batra Akber Abid Gardezi syed Immamul Ansarullah shafiq Ahmad Mejdal Alqahtani Muhammad shafiq National Institute of Electronics and Information Technology(NIELIT) J&K191132India Department of Computer science&Engineering Lovely Professional University(LPU)Punjab144402India Department of Computer science COMSATS University IslamabadIslamabad45550Pakistan Department of Computer science Govt.Degree College Sumbal Kashmir193501India Industrial Engineering Department College of EngineeringKing Saud UniversityP.O.Box 800Riyadh11421Saudi Arabia Department of Information and Communication Engineering Yeungnam UniversityGyeongsan38541Korea

A comprehensive understanding of human intelligence is still an ongoing process,i.e.,human and information security are not yet perfectly *** understanding cognitive processes,designers can design humanized cognitive information systems(CIs).The need for this research is j.s.ified because today’s business decision makers are faced with questions they cannot answer in a given amount of time without the use of cognitive information *** researchers aim to better strengthen cognitive information systems with more pronounced cognitive thresholds by demonstrating the resilience of cognitive resonant frequencies to reveal possible responses to improve the efficiency of human-computer interaction(HCI).Apractice-oriented research approach included research analysis and a review of existing articles to pursue a comparative research model;thereafter,amodel development paradigm was used to observe and monitor the progression of CIs during *** scope of our research provides a broader perspective on how different disciplines affect HCI and how human cognitive models can be enhanced to enrich *** have identified a significant gap in the current literature on mental processing resulting from a wide range of theory and practice.

关键词： Cognitive-IoT human-computer interaction decision making

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Homotopy solution for stagnation-Point Flow

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Communications in Theoretical Physics 2012年第2期57卷 290-294页

作者： T.Hayat Ansa Rafique M.Y.malik s.A.shehzad s.Obaidat Department of Mathematics Quaid-i-Azam University 45320Islamabad 44000Pakistan Department of Mathematics College of SciencesKing Saud UniversityP.O.Box 2455Riyadh 1145Saudi Arabia

This article reports the homotopy solution for stagnation point flow of a non-Newtonian *** incompressible second grade fluid impinges on the wall either orthogonally or *** resulting nonlinear problems have been solved by a homotopy analysis method(HAM).Convergence of the series solutions is *** solutions are compared with the numerical solutions presented in a study [***-Linear Mech.43(2008) 941].Excellent agreement is noted between the numerical and series solutions.

关键词：同伦分析滞点不可压缩流体非线性问题非线性力学流体流动数值解 HAM

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