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Unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neuropathology and behavioral deficits in parkinsonian rats withα-synucleinopathy

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Neural Regeneration Research 2024年第9期19卷 2057-2067页

作者： Bismark Gatica-Garcia Michael j.Bannon Irma Alicia Martínez-Dávila Luis O.Soto-Rojas David Reyes-corona Lourdes Escobedo Minerva Maldonado-Berny ME Gutierrez-Castillo Armando j.Espadas-Alvarez Manuel A.Fernandez-Parrilla juan U.Mascotte-Cruz CP Rodríguez-Oviedo Irais E.Valenzuela-Arzeta Claudia Luna-Herrera Francisco E.Lopez-Salas jaime Santoyo-Salazar Daniel Martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Laboratorio de Patogénesis Molecular Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Red de Medicina para la Educación y Desarrollo y la Investigación Científica de Iztacala(Red MEDICI) Carrera Médico CirujanoFacultad de Estudios Superiores IztacalaUniversidad Nacional Autónoma de MéxicoTlalnepantla de BazMéxico Nanoparticle Therapy Institute AguascalientesMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Escuela Nacional de Medicina y Homeopatía Instituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasInstituto Politécnico NacionalCiudad de MéxicoMéxico Departamento de Biología Molecular y Biotecnología Instituto de Investigaciones BiomédicasUniversidad Nacional Autónoma de MéxicoCiudad de MéxicoMéxico Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa de Nanociencias y Nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Parkinsonism by unilateral,intranigralβ-sitosterolβ-D-glucoside administration in rats is distinguished in that theα-synuclein insult begins unilaterally but spreads bilaterally and increases in severity over time,thus replicating several clinical features of Parkinson’s disease,a typicalα-synucleinopathy.As Nurr1 repressesα-synuclein,we evaluated whether unilateral transfected of rNurr1-V5 transgene via neurotensin-polyplex to the substantia nigra on day 30 after unilateralβ-sitosterolβ-D-glucoside lesion could affect bilateral neuropathology and sensorimotor deficits on day 30 post-transfection.This study found that rNurr1-V5 expression but not that of the green fluorescent protein(the negative control)reducedβ-sitosterolβ-D-glucoside-induced neuropathology.Accordingly,a bilateral increase in tyrosine hydroxylase-positive cells and arborization occurred in the substantia nigra and increased tyrosine hydroxylase-positive ramifications in the striatum.In addition,tyrosine hydroxylase-positive cells displayed less senescence markerβ-galactosidase and more neuron-cytoskeleton markerβIII-tubulin and brain-derived neurotrophic factor.A significant decrease in activated microglia(positive to ionized calcium-binding adaptor molecule 1)and neurotoxic astrocytes(positive to glial fibrillary acidic protein and complement component 3)and increased neurotrophic astrocytes(positive to glial fibrillary acidic protein and S100 calcium-binding protein A10)also occurred in the substantia nigra.These effects followed the bilateral reduction inα-synuclein aggregates in the nigrostriatal system,improving sensorimotor behavior.Our results show that unilateral rNurr1-V5 transgene expression in nigral dopaminergic neurons mitigates bilateral neurodegeneration(senescence and loss of neuron-cytoskeleton and tyrosine hydroxylase-positive cells),neuroinflammation(activated microglia,neurotoxic astrocytes),α-synuclein aggregation,and sensorimotor deficits.Increased neurotrophic astrocytes and brain-derived neurotrophic factor can mediate t

关键词： A1 astrocytes A2 astrocytes gene therapy microglia motor deficits nanoparticles neurodegeneration neuroinflammation senescence α-synuclein aggregates

Embryo and Endosperm Phytochemicals from Polyembryonic Maize Kernels and Their Relationship with Seed Germination

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Phyton-International journal of Experimental Botany 2022年第5期91卷 929-941页

作者： j.David García-ortíz Rebeca González-Centeno María Alejandra Torres-Tapia j.A.Ascacio-Valdés joséEspinoza-Velázquez Raúl Rodríguez-Herrera Food Research Department School of ChemistryAutonomous University of CoahuilaSaltillo25280México Plant Breeding Department Universidad Autonoma Agraria Antonio Narro(UAAAN)Saltillo25315México

Because of the growing worldwide demand for maize grain,new alternatives have been sought for breeding of this cereal,e.g.,development of polyembryonic varieties,which agronomic performance could positively impact the grain yield per unit area,and nutritional quality.The objectives of this study were to(1)determine the phytochemicals present in the embryo and endosperm of grain from maize families with high,low,and null polyembryony frequency,which were planted at different locations,and(2)state the relationship between these compounds and seed germination.The extracted phytochemicals from corn were identified by HPLC-MS.The results showed that the genotype with the highest presence of phytochemicals was the brachytic population with high polyembryony called“BAP”,which also required less water during the germination process.The number of phytochemicals in both embryo and endosperm tissues was not related to the sowing location where they came from or the type of polyembryony.The number of different phytochemicals depended on the grain tissue from where they were extracted.The chemical compounds found in the different maize tissues were related to the development of the plant,either in roots or nibs because these are mainly associated with the lignin synthesis.

关键词： zea mays L HPLC-MS locations kernels phytochemicals

Mechanical and histological properties of an electrospun scaffold with a modified surface by plasma polymerization implanted in an in vivo model

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BIOCELL 2022年第3期46卷 829-836页

作者： MARÍA G.FLORES-SÁNCHEz ROBERTO OLAYO j.MORALES-corona ATLÁNTIDA M.RAYA-RIVERA DIEGO R.ESQUILIANO-RENDÓN Research Vice-Rectory Faculty of EngineeringUniversity La Salle México06140México Department of Physics Metropolitan Autonomous University09340México Department of Tissue Engineering Child Hospital of México Federico Gómez06720México

This article presents the construction of scaffolds composed of polylactic acid(PLA)with different concentrations of hydroxyapatite(HA)by electrospinning,which were superficially modified with polypyrrole(PPy/I)by plasma polymerization.A preliminary study was conducted of the biological and mechanical behavior of the scaffolds when they were implanted in the back of rabbits for 30 days;bone cells differentiated from mesenchymal stem cells(MSCs)were used.The bone cell and scaffold structures were characterized by histological,immunohistochemical,and mechanical stress tests.Hematoxylin–eosin staining showed good tissue conformation.The immunohistochemical tests highlighted the presence of the main bone tissue proteins,such as collagen,osteocalcin,and osteopontin.The PLA/HA scaffolds were observed to exhibit cell adhesion and proliferation properties;however,the response was much better in the scaffolds that had a higher concentration of HA and that were coated with PPy/I.The results of the mechanical tests of the scaffolds indicated that the plasma treatment improved the adhesion and cell proliferation properties and contributed to the mechanical support,allowing the formation of neotissues with good viability of cell growth.

关键词： Hydroxyapatite Polylactic acid pyrrole Electrospinning Surface activation

Cerebral dopamine neurotrophic factor transfection in dopamine neurons using neurotensin-polyplex nanoparticles reverses 6-hydroxydopamine-induced nigrostriatal neurodegeneration

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Neural Regeneration Research 2022年第4期17卷 854-866页

作者： Manuel A.Fernandez-Parrilla David Reyes-corona Yazmin M.Flores-Martinez Rasajna Nadella Michael j.Bannon Lourdes Escobedo Minerva Maldonado-Berny jaime Santoyo-Salazar Luis O.Soto-Rojas Claudia Luna-Herrera jose Ayala-Davila juan A.Gonzalez-Barrios Gonzalo Flores Maria E.Gutierrez-Castillo Armando jEspadas-Alvarez Irma A.Martínez-Dávila Porfirio Nava Daniel Martinez-Fong Departamento de Fisiología Biofísica y NeurocienciasCentro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Programa Institucional de Biomedicina Molecular Escuela Nacional de Medicina y HomeopatíaInstituto Politécnico NacionalCiudad de MéxicoMéxico Department of Biosciences IIIT-SrikakulamRajiv Gandhi University of Knowledge Technologies(RGUKT)Andhra PradeshIndia Department of Pharmacology Wayne State University School of MedicineDetroitMIUSA Departamento de Física Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico Facultad de Estudios Superiores Iztacala Universidad Nacional Autónoma de MéxicoTlalnepantla de BazEdo.de MéxicoMéxico Departamento de Fisiología Escuela Nacional de Ciencias BiológicasCiudad de MéxicoMéxico Laboratorio de Medicina Genómica Hospital Regional“1°de Octubre”ISSSTECiudad de MéxicoMéxico Laboratorio de Neuropsiquiatría Instituto de FisiologíaBenemérita Universidad Autónoma de PueblaPueblaPueblaMéxico Departamento de Biociencias e Ingeniería Centro Interdisciplinario de Investigaciones y Estudios sobre Medio Ambiente y DesarrolloInstituto Politécnico NacionalCiudad de MéxicoMéxico Programa de Nanociencias y nanotecnología Centro de Investigación y de Estudios AvanzadosCiudad de MéxicoMéxico

Overexpression of neurotrophic factors in nigral dopamine neurons is a promising approach to reverse neurodegeneration of the nigrostriatal dopamine system,a hallmark in Parkinson's disease.The human cerebral dopamine neurotrophic factor(h CDNF)has recently emerged as a strong candidate for Parkinson's disease therapy.This study shows that h CDNF expression in dopamine neurons using the neurotensinpolyplex nanoparticle system reverses 6-hydroxydopamine-induced morphological,biochemical,and behavioral alterations.Three independent electron microscopy techniques showed that the neurotensin-polyplex nanoparticles containing the h CDNF gene,ranging in size from 20 to 150 nm,enabled the expression of a secretable h CDNF in vitro.Their injection in the substantia nigra compacta on day 21 after the 6-hydroxydopamine lesion resulted in detectable h CDNF in dopamine neurons,whose levels remained constant throughout the study in the substantia nigra compacta and striatum.Compared with the lesioned group,tyrosine hydroxylase-positive(TH^(+))nigral cell population and TH+fiber density rose in the substantia nigra compacta and striatum after h CDNF transfection.An increase inβIII-tubulin and growth-associated protein 43 phospho-S41(GAP43 p)followed TH^(+)cell recovery,as well as dopamine and its catabolite levels.Partial reversal(80%)of drugactivated circling behavior and full recovery of spontaneous motor and non-motor behavior were achieved.Brain-derived neurotrophic factor recovery in dopamine neurons that also occurred suggests its participation in the neurotrophic effects.These findings support the potential of nanoparticle-mediated h CDNF gene delivery to develop a disease-modifying treatment against Parkinson's disease.The Institutional Animal Care and Use Committee of Centro de Investigación y de Estudios Avanzados approved our experimental procedures for animal use(authorization No.162-15)on june 9,2019.

关键词： axonal growth brain-derived neurotrophic factor gene delivery nanoparticles neuritogenesis neuronal cytoskeleton neuroregeneration neurorestoration neurotrophic therapy Parkinson's disease reinnervation substantia nigra

An egg-shell bifunctional CeO_(2)-modified NiPd/Al_(2) O_(3) catalyst for petrochemical processes involving selective hydrogenation and hydroisomerization

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journal of Rare Earths 2021年第11期39卷 1382-1388,I0003页

作者： Franklin j.Mendez javier A.Alves Yahse Rojas-Challa Oscar corona Yanet Villasana julia Guerra German Garcia-Colli Osvaldo M.Martinez joaquin L.Brito Centro de Quimica Instituto Venezolano de Invesdgaciones CientfficasCarretera PanamericanaKm 11 Altos de PipeCaracas1020AVenezuela Departamento de Estado Solido Instituto de FisicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaCiudad de Mexico04510Mexico Departamento de Ingenieria Quimica Facultad de Ingenieria(UNLP)and Centro de Investigacion y Desarrollo en Ciencias Aplicadas"Dr.J.J.Ronco"(CINDECA)CCT-La Plata-CONICET-UNLPLa Plata1900Argentina Departamento de Quimica Analitica Facultad de QufmicaUniversidad Nacional Autonoma de MexicoCiudad UniversitariaCiudad de Mexico04510Mexico Instituto Tecnoldgico Venezolano Del Petroleo SA. Petrole os de Venezuela SA.Urb.Santa Rosa-Sector El TamborLos Teques1201Venezuela Instituto Superior de Formacion Docente Salome Urena Esquina Leonardo D'VinciSanto Domingo10114Republica Dominicana Biomass to Resources Group Universidad Regional Amazonica IkiamKm 7 via MuyunaTena150150Ecuador Departamento de Termodinamica y Fenomenos de Transferencia Universidad Simon BolivarValle de SartenejasCaracas1086Venezuela

The catalytic performance during the 1-butyne hydrogenation using two reduced Al_(2) O_(3)-supported Pdbased catalysts was carried out in a total recirculation system with an external fixed-bed reactor.The lab-prepared egg-shell NiPd/CeO_(2)-Al_(2) O_(3) catalyst(NiPdCe) with Pd loading=0.5 wt%,Ni/Pd atomic ratio=1 and CeO_(2) loading=3 wt% was synthesized and characterized,and it was compared with an egg-shell Al_(2) O_(3)-supported Pd based commercial catalyst(PdCC).The reduced catalysts were characterized by X-ray diffraction,X-ray photoelectron spectroscopy,and high-resolution transmission electron microscopy.The textural characteristics and ammonia temperature-programmed desorption profiles of the fresh(unreduced) catalysts were also obtained.Both catalysts show high 1-butyne conversion and selectivity to 1-butene,but the catalysts also present important differences between hydroisomerizing and hydrogenating capabilities.NiPdCe catalyst shows higher capability for hydroisomerization reactions,while the PdCC catalyst exhibits higher hydrogenating capability.The observed catalytic performances can be interesting for some industrial processes and can provide a guideline for the development of a Pd-based catalyst with specific catalytic properties.

关键词： 1-Butyne Bifunctional catalyst Egg-shell Hydrogenation Hydroisomerization NiPd catalyst

Data-driven Simulations of Magnetic Field Evolution in Active Region 11429:Magneto-frictional Method Using PENCIL CODE

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Research in Astronomy and Astrophysics 2024年第2期24卷 74-82页

作者： P.Vemareddy jorn Warnecke Ph.A.Bourdin Indian Institute of Astrophysics Sarjapur roadII BlockKoramangalaBengaluru-560034India Max-Planck-Institut für Sonnensystemforschung Justus-von-Liebig-Weg 3D-37077 GöttingenGermany Institute of Physics University of GrazUniversitätsplatz 5A-8010 GrazAustria

coronal magnetic fields evolve quasi-statically over long timescales and dynamically over short timescales.As of now there exist no regular measurements of coronal magnetic fields,and therefore generating the coronal magnetic field evolution using observations of the magnetic field at the photosphere is a fundamental requirement to understanding the origin of transient phenomena from solar active regions(ARs).Using the magneto-friction(MF)approach,we aim to simulate the coronal field evolution in the solar AR 11429.The MF method is implemented in the open source PENCIL CODE along with a driver module to drive the initial field with different boundary conditions prescribed from observed vector magnetic fields at the photosphere.In order to work with vector potential and the observations,we prescribe three types of bottom boundary drivers with varying free-magnetic energy.The MF simulation reproduces the magnetic structure,which better matches the sigmoidal morphology exhibited by Atmospheric Imaging Assembly(AIA)images at the pre-eruptive time.We found that the already sheared field further driven by the sheared magnetic field will maintain and further build the highly sheared coronal magnetic configuration,as seen in AR 11429.Data-driven MF simulation is a viable tool to generate the coronal magnetic field evolution,capturing the formation of the twisted flux rope and its eruption.

关键词： Sun corona-Sun evolution-Sun magnetic fields-Sun photosphere

Thermal Comparison of an Organic Rankine Cycle Activated by a Low-Temperature Geothermal Heat Source

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journal of Mechanics Engineering and Automation 2018年第7期8卷 309-315页

作者： Vera-Romero Iván corona-Ruíz Silvia L. Martínez Reyes j. Moreno Nava I. Ordaz Murillo O. Méndez á brego Víctor M Department of Energy Engineering University of the Ciénega of Michoacán State Sahuayo 59103 Mexico Department of Multicultural Studies University of the Ciénega of Michoacán State Sahuayo 59103 Mexico

This study compares the results from the thermodynamic analysis at an Organic Rankine Cycle power plant,for first and second law efficiency,operating with 14 different working fluids.This plant—located in the town of Los Negritos,in the municipality of Villamar,in the State of Michoacán,Mexico—uses heat from a low-temperature geothermal source with the purpose of identifying the working fluids that are best suited for the operating conditions at hand.REFPROP v.8.0 software was used to collect the thermodynamic properties.Of all the thermodynamic parameters that were analyzed,the fluid that performed the best had an overall energy efficiency of 5.87%,an overall exergy efficiency of 43.07%,and a net output power of 13.04 kWe.

关键词： Organic Rankine cycle geothermal energy Los Negritos thermodynamic analysis exergy

Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test

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Advances in Microbiology 2016年第13期6卷 959-964页

作者： josé L. corona-Vargas Myrna A. Vicencio-Mallén Frida Salmerón-Sosa Erika M. Carrillo-Casas Francisco j. Trigo-Tavera Rosa E. Miranda-Morales Departamento de Microbiología e Inmunología Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México Circuito Exterior s/n Ciudad Universitaria Coyoacán México Departamento de Biología Molecular e Histocompatibilidad Dirección de Investigación Hospital General Dr. Manuel Gea González Calz Tlalpan México Departamento de Patología Facultad de Medicina Veterinaria y ZootecniaUniversidad Nacional Autónoma de México Circuito Exterior s/n Ciudad Universitaria Coyoacán México

Mycoplasma mycoides subsp. capri is the causative agent of severe and acute respiratory problems in goats, which spreads rapidly and represents high mortality. The serological profile of the goat population, from nine regions in seven states of Mexico, was screened by the Complement Fixation test (CF) in sera from asymptomatic goats and animals with mild respiratory symptoms. Sera and nasal swabs of 827 goats were collected for the isolation of the organism. An antiserum was prepared against a previously isolated field strain of Mycoplasma mycoides subsp. capri. CF Antibody titers were associated with the results of the isolates to determine the cutoff point. The CF was considered as positive if its result was ≥1/16. The CF registered 251 positive goats (30.35%) and 576 (69.65%) negative;the test showed high sensitivity (93.33%) and specificity (72.27%). In the specific case of diagnosis for mycoplasmosis associated with respiratory problems in goats, the CF proved to be a good diagnosis test, this study determined that 30% of the goat population showed antibody titers against Mycoplasma mycoides subsp. capri and revealed those animals who have had contact with this microorganism during their lives regardless of the presence or absence of respiratory symptoms.

关键词： Complement Fixation Indirect Agglutination Mycoplasmosis Mycoplasma mycoides Mycoplasma mycoides subsp. capri

Group B Streptococcal Chorioamnionitis with Intact Amniotic Membranes. A Rare?

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Open journal of Obstetrics and Gynecology 2020年第6期10卷 809-814页

作者： j. L. Gallo Vallejo C. Rodríguez-Rubio corona M. F. Hurtado Sánchez A. Alkourdi Martínez L. Revelles Paniza Obstetrics and Gynecology Service Virgen de las Nieves University Hospital Granada Spain

Group B streptococcus infection (GBS) is an important cause of perinatal infection. Maternal colonization by GBS can cause amniotic infection and thus fetal infection, sometimes causing fetal death. This infection generally occurs in association with premature rupture of membranes. We present here an infrequent case of GBS chorioamnionitis in full-term gestation with intact amniotic membranes. The curiosity of the case lies in the integrity of the amniotic membranes, the asymptomatic clinical presentation, and the adverse result (term fetal death).

关键词： Chorioamnionitis Group B Streptococcus Intact Amniotic Membranes