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calcineurin-B-Like Protein cBL9 Interacts with Target Kinase cIPK3 in the Regulation of ABA Response in Seed Germination

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Molecular Plant 2008年第2期1卷 238-248页

作者： Girdhar K. pandey john j. Grant Yong Hwa cheong Beom-Gi Kim Le Gong Li Sheng Luan Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA Department of Plant Molecular Biology University of Delhi South Campus Benito Juarez Road New Delhi-110021 India Present address: Department of Bio-Environmental Science Sunchon National University Suncheon Jeonnam 540-742 Korea Present address: Department of Molecular Physiology and Biochemistry National institute of Agricultural Biotechnology Suwon 441-707 Korea

calcium plays a vital role as a second messenger in many signaling pathways in plants. The calcineurin B-like proteins （cBLs） represent a family of plant calcium-binding proteins that function in calcium signaling by interacting with their interacting protein kinases （cIPKs）. In our previous study, we have reported a role for one of the cBLs （cBL9） and one of the cIPKs （cIPK3） in ABA signaling. Here, we have shown that cBL9 and cIPK3 physically and functionally interact with each other in regulating the ABA responses. The cBL9 and cIPK3 proteins interacted with each other in the yeast two- hybrid system and when expressed in plant cells. The double mutant cbl9cipk3 showed the similar hypersensitive response to ABA as observed in single mutants （cbl9 or cipk3）. The constitutively active form of cIPK3 genetically complemented the cbl9 mutant, indicating that cIPK3 function downstream of cBL9. Based on these findings, we conclude that cBL9 and cIPK3 act together in the same pathway for regulating ABA responses.

关键词： ABA calcium cBL cIPK osmotic stress signal transduction.

A robust deep structured prediction model for petroleum reservoir characterization using pressure transient test data

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Petroleum Research 2022年第2期7卷 204-219页

作者： Rakesh Kumar pandey Anil Kumar Ajay Mandal Department of Petroleum and Energy Studies School of Engineering and TechnologyDIT UniversityDehradun248009India Data Science Research Group School of ComputingDIT UniversityDehradun248009India Department of Petroleum Engineering Indian Institute of Technology(Indian School of Mines)Dhanbad826004India

A robust deep learning model consisting of long short-term memory and fully connected neural net-works has been proposed to automatically interpret homogeneous petroleum reservoirs having infinite,no flow,and constant pressure outer boundary *** pressure change data recorded during the well test operation along with its derivative is input into the model to perform the classification for identifying the reservoir model and,further,regression to estimate output *** noise was added to analytical models while generating the synthetic training *** hyperparameters were regulated to perform model optimization,resulting in a batch size of 64,Adam optimization algorithm,learning rate of 0.01,and 80:10:10 data split ratio as the best choices of *** perfor-mance accuracy also increased with an increase in the number of samples during *** classification and regression metrics have been used to evaluate the performance of the *** paper also demonstrates the prediction performance of the optimized model using simulated and actual oil well pressure drawdown test *** proposed model achieved minimum and maximum relative errors of 0.0019 and 0.0308,respectively,in estimating output for the simulated test cases and relative error of 0.0319 for the real test case.

关键词： Well test Reservoir characterization Automatic interpretation Prediction model Hyperparameter tuning Performance indicator

Evaluation of reference evapotranspiration methods for the northeastern region of India

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International Soil and Water conservation Research 2016年第1期4卷 52-63页

作者： Pankaj K.pandey Parmendra PDabral Vanita pandey Department of Agricultural Engineering North Eastern Regional Institute of Science and Technology(NERIST)NirjuliItanagarArunachal PradeshIndia Department of Soil and Water Engineering College of Agricultural Engineering and Post-Harvest Technology(CAEPHT)RanipoolGangtokSikkimIndia

The study planed to identify a suitable alternative to the FAO 56 Penman-Monteith(FAO56PM)equation for calculating reference evapotranspiration(ET_(0))from chosen te mperature and radiation based models utilizing monthly meteorological data from 30 destinations in diverse agro-ecologial regions of the Northeast(NE)India ie,Assam Bengal Plain(ABP),eastern Himalaya(EH),and the northeastern hilly(NEH)***-based IRMAK3 most appropriate in the ABP(weighted root mean square deviation,WRMSD=0.17 mm d^(-1),r^(2)=0.98,for Nagrakata),and TURc model being in the first three rank of most of the sites,with the lowest error and highest correlation in NEH(WRMSD=0.10 mm d^(-1),r^(2)=0.92,for Shillong),and EH(WRMSD=0.23 mm d^(-1),r^(2)=-0.95,for Gangtok).Findings reveal that IRMAK3 and TURc models performed equally well and were observed to be the best among selected modets for the majority of stations followed by FAO24 Blaney-criddle(FAO24Bc),and 1957MAKK Pair-wise regession equations were developed for preferred FAO56PM ET_(0) estimates to ET_(0) estimates by alternative ***-correlation of eighteen chose methods demonstrated that the five equations(*** radiation-and one temperature-based)performed eceptionally well when contrasted with the FAO56PM model,thus being advised for assessing ET_(0)。under limiting data conditions as have yielded a better estimate of ET_(0) with a small error.

关键词： Reference evapotranspiration Performance statistics calibration and validation Empirical methods North East India

A Novel convolutional Neural Networks Based Spinach classification and Recognition System

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computers, Materials & continua 2022年第10期73卷 343-361页

作者： Sankar Sennan Digvijay pandey Youseef Alotaibi Saleh Alghamdi Department of computer Science and Engineering Sona College of TechnologySalem636005India Department of Technical Education Department of Electronics EngineeringInstitute of Engineering and TechnologyDr.A.P.J.Abdul Kalam Technical UniversityLucknowIndia Department of computer Science College of Computer and Information SystemsUmm Al-Qura UniversityMakkah21955Saudi Arabia Department of Information Technology College of Computers and Information TechnologyTaif UniversityTaif21944Saudi Arabia

In the present scenario,Deep Learning(DL)is one of the most popular research algorithms to increase the accuracy of data *** to intra-class differences and inter-class variation,image classification is one of the most difficult jobs in image *** or spinach recognition or classification is one of the deep learning applications through its *** is more critical for human skin,bone,and hair,*** provides vitamins,iron,minerals,and *** is beneficial for diet and is readily available in people’s *** researchers have proposed various machine learning and deep learning algorithms to classify plant images more accurately in recent *** paper presents a novel convolutional Neural Network(cNN)to recognize spinach more *** proposed cNN architecture classifies the spinach category,namely Amaranth leaves,Black nightshade,curry leaves,and Drumstick *** dataset contains 400 images with four classes,and each type has 100 *** images were captured from the agricultural land located at Thirumanur,Salem district,Tamil *** proposed cNN achieves 97.5%classification *** addition,the performance of the proposed cNN is compared with Support Vector Machine(SVM),Random Forest,Visual Geometry Group 16(VGG16),Visual Geometry Group 19(VGG19)and Residual Network 50(ResNet50).The proposed provides superior performance than other models,namely SVM,Random Forest,VGG16,VGG19 and ResNet50.

关键词： Accuracy convolutional deep learning plant neural networks spinach

Synergistic Effect of Metal Nanoparticles on the Antimicrobial Activities of Antibiotics against Biorecycling Microbes

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journal of Materials Science & Technology 2016年第6期32卷 524-532页

作者： chandni Khurana Purnima Sharma O.E pandey Bhupendra chudasama Laboratory of Nanomedicine School of Physics and Materials ScienceThapar UniversityPatiala 147004India Department of Applied Sciences Chandigarh UniversityMohali 160055India Department ofBiotechnology Thapar UniversityPatiala 147004India

Biorecycling microbes, which have critical functionalities in natural cycles, are essential to sustain eco-system of the earth. Any alterations in these cycles caused by the mutations of microbes could be a potential threat to life on earth. Antibiotics leached from pharmaceutical waste, animal food and agribusiness prod- ucts are accumulating in the environment. Metal nanoparticles are also accumulating in environment because of their extensive use as biocidal agent in domestic products. Interaction of antibiotics and metal nanoparticles with eco-ffiendly microorganisms has a potential to alter the ecosystem of the earth. In this article, we have studied the antibacterial activities of silver and copper nanoparticles and their for- mulations with antibiotics, tetracycline, and kanamycin against biorecycling microbes, Bacillus subtilis and Pseudomonas fluorescens. Strong synergistic effect of metal nanoparticles on the antimicrobial ac- tivities of commercial antibiotics has been observed. Antimicrobial activity of tetracycline improves by 286%-346% and 0%-28% when being tested in the presence of 250 ppm of silver and copper nanoparticles, respectively. For kanamycin, the improvement is 154%-289% for silver and 3%-20% for copper nanoparticles. Irrespective of the antibiotics and tested organisms, synergy is more prominent for silver nanoparticles even at their minimum active concentration （100 ppm）. This study demonstrates that the combination of metal nanoparticles with antibiotics could be more fatal to ecosystem than either the metal nanoparticles or the antibiotics alone.

关键词： Synergy Nanoparticles Antibiotics Biorecycling microbes Antibacterial activities

Recent development in upconversion nanoparticles and their application in optogenetics:A review

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journal of Rare Earths 2022年第6期40卷 847-861,I0001页

作者： Madhumita Patel Maninder Meenu jitender Kumar pandey Pawan Kumar Rajkumar Patel Department of chemistry and Nano Science Ewha Womans UniversitySeodaemun-guSeoul120-750South Korea college of Biosystems Engineering and Food Science Zhejiang UniversityHangzhou 310058China School of Basic and Applied Sciences Adamas UniversityKolkata700126India Institut National de la Recherche Scientifique-centreénergie Materiaux Télecommunications(INRS-EMT) VarennesQCCanada Department of chemistry and Biochemistry University of Oklahoma101 Stephenson ParkwayNormanOK73019United States Energy&Environmental Science and Engineering(EESE) Integrated Science and Engineering Division(ISED)Underwood International CollegeYonsei University85 Songdogwahak-roYeonsu-guIncheon21983South Korea

Ion channels present in the plasma membrane are responsible for integration and propagation of electric signals,which transmit information in nerve *** of these ion channels leads to many neurological ***,optogenetic technology has gained a lot of attention for the manipulation of neuronal *** is a neuromodulation approach that has been developed to control neuronal functions and activities using *** lanthanide-doped upconversion nanoparticles(UcNPs)absorb low energy photons in near-infrared(NIR) window and emit high energy photons in the visible spectrum region via nonlinear *** the last few decades,UcNPs have gained great attention in various bio-medical applications such as bio-imaging,drug delivery and *** near-infrared illumination is considered more suitable for optogenetics application,due to its lower degree of light attenuation and higher tissue penetration compared to visible ***,UcNPs have been considered as the new promising candidates for optogenetics *** nanoparticlemediated optogenetic systems provide a great opportunity to manipulate the ion channel in deep ***,we summarize the upconversion photoluminescence in lanthanide doped nanomaterials and its mechanisms and several approaches adopted to tune emission color or enhance upconversion *** advances of lanthanide-doped UcNPs design strategy and their mechanism are ***,we discuss the neural circuitry modulation using upconversion nanoparticles mediated ***,the future perspectives towards optogenetics are also included.

关键词： Lanthanide Upconversion Optogenetics Nanoparticles Neuronal modulation Rare earths

An exploration of frictional and vibrational behaviors of textured deep groove ball bearing in the vicinity of requisite minimum load

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Friction 2021年第6期9卷 1749-1765页

作者： K.E.ch.VIDYASAGAR R.K.pandey Dinesh KALYANASUNDARAM center for Biomedical Engineering Indian Institute of Technology DelhiNew Delhi 110016India Department of Biomedical Engineering College of EngineeringOsmania UniversityTelangana 500007India Department of Mechanical Engineering Indian Institute of Technology DelhiNew Delhi 110016India Department of Biomedical Engineering Indian Institute of Technology DelhiNew Delhi 110016India

In case of lightly loaded radial ball bearings,failure mechanisms other than fatigue such as smearing of raceways due to increased frictional torque and vibrations often ***,attempts have been made herein for reducing the frictional torque and minimizing the vibrations of a radial deep groove ball bearing employing surface textures at the inner *** pulsed laser was used to create texture(involving micro-dimples having different dimple area density)on the inner race of test *** an in-house developed test rig,frictional torque and vibrational parameters were measured at different speeds and light loads(*** vicinity of 0.01c,where c is dynamic load capacity of radial ball bearing).Significant reduction in frictional torque and overall vibrations were found in the presence of micro-dimples on inner race at light loads irrespective of operating *** without satisfying the minimum load needed criteria for the satisfactory operation,substantial reduction in smearing marks was found on the races of textured ball bearings in comparison to conventional cases.

关键词： ball bearing textured inner race grease lubricated frictional torque vibration

A detailed study on trust management techniques for security and privacy in IoT:challenges,trends,and research directions

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High-confidence computing 2023年第2期3卷 71-82页

作者： Himani Tyagi Rajendra Kumar Santosh Kr pandey Department of computer Science Jamia Millia IslamiaNew Delhi 110025India Department of Ec&IT MeityNew Delhi 110003India

The Internet of Things is a modern technology that is directed at easing human life by automating most of the things used in daily *** never-ending dependency on the network for communication is attracting adversaries to exploit the vulnerabilities of ***,this technology is facing some serious issues and challenges concerning security and *** issues and challenges are the real motivation behind considering this ***,this survey includes a discussion about security and privacy challenges as well as available solutions for IoT based wireless sensor *** systematic literature review(SLR)focuses particularly on a popular and applicable security approach known as Trust Management System(TMS).Firstly,all aspects of trust management,including trust indicators,trust properties,trust evaluation,trust building,trust models and the importance of those models for security and privacy,trust prediction methodologies,and ultimately trust-based attacks,are covered in this ***,trust management schemes are classified into four groups based on the methodology used for trust-based security solutions in the IoT:cryptography-based,computational and probabilistic-based,information theory-based,and ***,an understanding of the problems and difficulties with current methodologies is given,along with suggestions for further ***,the SLR is concluded by formulating the desirable characteristics of a trust management system in the IoT and proposing a trust model suitable for IoT networks.

关键词： Trust Trust management approaches IoT Security Privacy Reliability Attacks Trust attributes Systematic literature review cybersecurity

Whole genome phylogeny of Gallus: introgression and data-type effects

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Avian Research 2020年第1期11卷 1-15页

作者： George P.Tiley Akanksha pandey Rebecca T.Kimball Edward L.Braun j.Gordon Burleigh Department of Biology University of FloridaGainesvilleFL 32611USA Department of Biology Duke UniversityDurhamNC 27708USA

Background: Previous phylogenetic studies that include the four recognized species of Gallus have resulted in a number of distinct topologies, with little agreement. Several factors could lead to the failure to converge on a consistent topology, including introgression, incomplete lineage sorting, different data types, or insufficient ***: We generated three novel whole genome assemblies for Gallus species, which we combined with data from the published genomes of Gallus gallus and Bambusicola thoracicus(a member of the sister genus to Gallus). To determine why previous studies have failed to converge on a single topology, we extracted large numbers of orthologous exons, introns, ultra-conserved elements, and conserved non-exonic elements from the genome assemblies. This provided more than 32 million base pairs of data that we used for concatenated maximum likelihood and multispecies coalescent analyses of ***: All of our analyses, regardless of data type, yielded a single, well-supported topology. We found some evidence for ancient introgression involving specific Gallus lineages as well as modest data type effects that had an impact on support and branch length estimates in specific analyses. However, the estimated gene tree spectra for all data types had a relatively good fit to their expectation given the multispecies ***: Overall, our data suggest that conflicts among previous studies probably reflect the use of smaller datasets(both in terms of number of sites and of loci) in those analyses. Our results demonstrate the importance of sampling large numbers of loci, each of which has a sufficient number of sites to provide robust estimates of gene trees. Low-coverage whole genome sequencing, as we did here, represents a cost-effective means to generate the very large data sets that include multiple data types that enabled us to obtain a robust estimate of Gallus phylogeny.

关键词： Galliformes Incomplete lineage sorting junglefowl Multispecies coalescent Phasianidae Phylogenomics Species tree