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Design and synthesis of synthetic promoters for consistency of gene expression across growth phases and scale in ***

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Synthetic and Systems Biotechnology 2024年第2期9卷 330-339页

作者： Kristin V.Presnell Omar Melhem Sarah M.coleman Nicholas j.Morse Hal S.Alper McKetta Department of chemical Engineering The University of Texas at Austin200E Dean Keeton St.Stop C0400AustinTX78712USA Institute for cellular and Molecular Biology The University of Texas at Austin2500 Speedway AvenueAustinTX78712USA

Metabolic engineering and synthetic biology endeavors benefit from promoters that perform consistently(or robustly)with respect to cellular growth phase(exponential and stationary)and fermentation scale(microtiter plates,tubes,flasks,and bioreactors).However,nearly all endogenous promoters(especially in Saccharomyces cerevisiae)do not perform in this *** this work,a hybrid promoter engineering strategy is leveraged to create novel synthetic promoters with robustness across these *** a multi-dimensional RNA-seq dataset,promoters with specific phase dependencies were *** enriched with functional transcription factors were identified using MEME *** motif-containing fragments could impart activity dependence in the opposing ***,we obtain two new promoters with high and consistent expression across both phases by increasing the exponential phase activity of the starting stationary-phase scaffold by 38 and 23-fold ***,we show that these promoters function consistently across various laboratory growth scales over time in a microtiter plate and in ***,this work presents and validates a new strategy for engineering promoters in *** with high levels of expression that are robust to cellular growth phase and the scale of the culture.

关键词： endogenous synthetic stationary

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黄河下游叶蜡正构烷烃的物源指示意义

中国科学：地球科学 2024年第6期54卷 2031-2049页

作者：张亮刘金召姜崇张飞安芷生西安交通大学人居环境与建筑工程学院西安710054 中国科学院地球环境研究所黄土与第四纪地质国家重点实验室西安710061 陕西黄土高原地球关键带国家野外科学观测研究站西安710061

黄河作为输沙量和有机碳输送通量最大的河流之一,在区域生物地球化学及全球碳循环过程中发挥了至关重要的作用.目前,关于黄河总颗粒有机碳的通量、组成、来源及其季节变化已开展了大量研究,但针对不同时空尺度上分子生物标志物的研究仍... 详细信息

黄河作为输沙量和有机碳输送通量最大的河流之一,在区域生物地球化学及全球碳循环过程中发挥了至关重要的作用.目前,关于黄河总颗粒有机碳的通量、组成、来源及其季节变化已开展了大量研究,但针对不同时空尺度上分子生物标志物的研究仍然较少.本文重点研究了黄河下游不同季节(汛期、非汛期),不同类型载体中叶蜡正构烷烃的分子组成及氢同位素比值.结果表明:岸边表土中叶蜡正构烷烃的分子指标反映了其上覆植被的输入;汛期悬浮物的正构烷烃分子指标体现出黄土高原不同地质时期土壤的混合特征,而受水动力条件影响,非汛期悬浮物表现出相应的季节性差异;另外,考虑气候因素影响,利用正构烷烃氢同位素对汛期悬浮物中单子叶植物的输入进行了半定量化估算.本研究一定程度上为黄河有机质的物源研究中叶蜡指标的应用提供了新的见解.

关键词：分子组成氢同位素叶蜡正构烷烃悬浮物黄河下游

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carbon Monoxide as c1 Building Block in Fine chemical Synthesis

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chinese journal of chemistry 2024年第2期42卷 199-221页

作者： Rui M.B.carrilho Mario j.F.calvete Gabor Mikle Laszlo Kollar Mariette M.Pereira University of coimbra Coimbra Chemistry CentreDepartment of ChemistryRua Larga3004-535 CoimbraPortugal Department of General and Inorganic chemistry and ELKH-PTE Research Groupfor Selective chemical Syntheses University of PecsIfjusag utja 6H-7624 PecsHungary

carbon monoxide(cO)has become one of the most relevant and versatile renewable c1 building blocks for chemical synthesis,especially in the fine chemicals industry,due to the development of efficient and selective catalysts for its *** this review,we present a comprehensive critical analysis of the last 10 years literature on the use of cO as a renewable feedstock for fine chemicals *** review is organized by type of catalytic reaction,namely alkene and alkyne carbonylation,hydroformylation,carbonylation of aryl halides,carbonylative cross-coupling and c-H *** examples of the synthesis of relevant building blocks and/or known pharmaceuticals are *** is placed on examples of utilizing cO as the c1 building block in one or more catalytic *** catalyst used and the reaction conditions are consistently presented throughout all of the examples.

关键词： carbon monoxide Fine chemicals carbonylation Hydroformylation c-H functionalization

HDPairFinder:A data processing platform for hydrogen/deuterium isotopic labeling-based nontargeted analysis of trace-level amino-containing chemicals in environmental water

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journal of Environmental Sciences 2024年第2期136卷 583-593页

作者： Tingting Zhao Kristin carroll caley B.craven Nicholas j.P.Wawryk Shipei Xing jian Guo Xing-Fang Li Tao Huan Department of chemistry Faculty of ScienceUniversity of British ColumbiaVancouver Campus2036 Main MallVancouverBritish ColumbiaV6T 1Z1Canada Division of Analytical and Environmental Toxicology Department of Laboratory Medicine and PathologyFaculty of Medicine and DentistryUniversity of AlbertaEdmontonAlberta T6G 2G3Canada

The combination of hydrogen/deuterium(H/D)formaldehyde-based isotopic methyl labeling with solid-phase extraction and high-performance liquid chromatography–high resolution mass spectrometry(HPLc-HRMS)is a powerful analytical solution for nontargeted analysis of trace-level amino-containing chemicals in water *** the huge amount of chemical information generated in HPLc-HRMS analysis,identifying all possible H/Dlabeled amino chemicals presents a significant challenge in data *** address this,we designed a streamlined data processing pipeline that can automatically extract H/D-labeled amino chemicals from the raw HPLc-HRMS data with high accuracy and ***,we developed a cross-correlation algorithm to correct the retention time shift resulting from deuterium isotopic effects,which enables reliable pairing of H-and D-labeled ***,we implemented several bioinformatic solutions to remove false chemical features generated by in-source fragmentation,salt adduction,and natural13c ***,we used a data mining strategy to construct the AMINES library that consists of over 38,000 structure-disjointed primary and secondary amines to facilitate putative compound ***,we integrated these modules into a freely available R program,*** rationale of each module was justified and its performance tested using experimental H/D-labeled chemical standards and authentic water *** further demonstrated the application of HDPairFinder to effectively extract N-containing contaminants,thus enabling the monitoring of changes of primary and secondary N-compounds in authentic water *** is a reliable bioinformatic tool for rapid processing of H/D isotopic methyl labeling-based nontargeted analysis of water samples,and will facilitate a better understanding of N-containing chemical compounds in water.

关键词： Isotope labeling Nontargeted analysis Reactive N-compounds HPLc-HRMS Source water

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2024年journal of Diabetes第8期中文导读

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中华内分泌代谢杂志 2024年第9期40卷 808-810页

作者： j Diabetes编辑部不详

论著/Original Article。三酰甘油-葡萄糖指数和超敏c反应蛋白对慢性冠脉综合征患者心血管结局的联合作用:多中心队列研究。The combined effect of triglyceride-glucose index and high-sensitivity c-reactive protein on cardiovasc... 详细信息

关键词：超敏c反应蛋白冠脉综合征三酰甘油

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早期负重与延迟负对踝关节骨折术后患者影响的临床研究

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中国临床药理学杂志 2024年第16期40卷 2343-2343页

作者： BRETHERTON c P AcHTEN j jOGARAH V 海峰

脚踝骨折手术后,患者通常被要求在6周内避免行走(延迟负重)。手术后2周步行(早期负重)可能是一种安全且可取的康复方案。本研究旨在对比早期负重康复方案与延迟负重康复方案的临床和成本效益本研究为一项随机、非劣效性对照的多中心临... 详细信息

脚踝骨折手术后,患者通常被要求在6周内避免行走(延迟负重)。手术后2周步行(早期负重)可能是一种安全且可取的康复方案。本研究旨在对比早期负重康复方案与延迟负重康复方案的临床和成本效益本研究为一项随机、非劣效性对照的多中心临床试验,研究对象为接受了不稳定踝关节骨折的急性手术的患者,被随机分为延迟负重组和早期负重组。

关键词：康复方案多中心临床试验踝关节骨折早期负重成本效益非劣效性骨折手术后急性手术

Temperature and structure measurements of heavy-ion-heated diamond using in situ X-ray diagnostics

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Matter and Radiation at Extremes 2024年第4期9卷 80-89页

作者： j.Lutgert P.Hesselbach M.Schorner V.Bagnoud R.Belikov P.Drechsel B.Heuser O.SHumphries P.Katrik B.Lindqvist c.Qu R.Redmer D.Riley G.Schaumann S.Schumacher A.Tauschwitz D.Varentsov K.Weyrich X.Yu B.Zielbauer Zs.Major P.Neumayer D.Kraus Institut fur Physik Universitat RostockAlbert-Einstein-Str.2318059 RostockGermany GSI Helmholtzzentrum fur Schwerionenforschung Planckstr.164291 DarmstadtGermany Institut für Angewandte Physik Goethe-Universität Frankfurt am MainMax-von-Laue-Str.160438 Frankfurt am MainGermany Institut fur Geowissenschaften Goethe-Universitat Frankfurt am MainAltenhoferallee 160438 Frankfurt am MainGermany Helmholtz-Zentrum Dresden-Rossendorf Bautzner Landstr.40001328 DresdenGermany European XFEL GmbH Holzkoppel 422869 SchenefeldGermany centre for Plasma Physics School of Mathematics and PhysicsQueen’s University BelfastUniversity RoadBelfast BT71NNUnited Kingdom Institut für Kernphysik Technische Universität DarmstadtSchlossgartenstr.964289 DarmstadtGermany State Key Laboratory of Nuclear Physics and Technology Institute of Heavy Ion PhysicsPeking UniversityBeijing 100871China Helmholtz Institut jena Frobelstieg 307743 JenaGermany

We present in situ measurements of spectrally resolved X-ray scattering and X-ray diffraction from monocrystalline diamond samples heatedwith an intense pulse of heavy *** this way,we determine the samples’heating dynamics and their microscopic and macroscopic structuralintegrity over a timespan of several *** the ratio of elastic to inelastic scattering with state-of-the-art density functionaltheory molecular dynamics simulations allows the inference of average temperatures around 1300 K,in agreement with predictions fromstopping power *** simultaneous diffraction measurements show no hints of any volumetric graphitization of the material,butdo indicate the onset of fracture in the diamond *** experiments pave the way for future studies at the Facility for Antiproton andIon Research,where a substantially increased intensity of the heavy ion beam will be available.

关键词： heated intense structure

Population Dynamics and Key Influencing Factors of Microthrix parvicella caused Sludge Bulking

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Huanjing Kexue/Environmental Science 2024年第11期45卷 6766-6774页

作者： Su G.-X. Qi R. Xiao S.-M. Dou D.-c. Guo Y.-X. Gao j. Tianjin Key Laboratory of Aquatic Science and Technology School of Environmental and Municipal Engineering Tianjin Chengjian University Tianjin 300384 China State Key Laboratory of Environmental Aquatic chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

Given the frequent occurrence of the sludge bulking phenomena caused by Microthrix parvicella（M. parvicella）overgrowth during the low-temperature period in the full-scale municipal wastewater treatment plant，the filamentous bacteria dynamics of the M. parvicella population were systematically investigated during the completed bulking period using the multi-dimensional integrated filamentous monitoring method combined with the molecular quantitative method. A lab-scale reactor was constructed and successfully simulated bulking phenomena under the low-temperature condition. The trend of dominant filamentous bacterial shift and the main influencing parameters were estimated during the bulking period，to verify the key factors for filament growth and extinction of the sludge bulking phenomenon in the full-scale plant. The results showed that the bulking-caused M. parvicella was characterized by significant changes in the diversity of filamentous bacteria，and the dominant filamentous bacterial succession was reflected in the competition between the skeleton filamentous bacteria Type 0092 and Type 0041 and M. parvicella. At lower temperatures，sludge loading was an important controlling factor for the competitive growth advantage of M. parvicella：when the temperature was lower（approximately 15°c），low sludge loading［0.05 kg·（kg·d）−1］could lead to the stabilized elevation of M. parvicella，and with the loading increased to 0.1 kg·（kg·d）−1 or more，M. parvicella populations showed a targeted decline despite the presence of the low temperature factor. © 2024 Science Press. All rights reserved.

关键词： filamentous bacteria Microthrix parvicella population dynamics sludge bulking sludge loading

Microglial glutaminase 1 mediates chronic restraint stress-induced depression-like behaviors and synaptic damages

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Signal Transduction and Targeted Therapy 2024年第1期9卷 40-43页

作者： Huili chen Shengyang Fu Xiangyu Li Meng Shi jiazhen Qian Shu Zhao Ping Yuan Lu Ding Xiaohuan Xia jialin c.Zheng center for Translational Neurodegeneration and Regenerative Therapy Tongji Hospital affiliated to Tongji University School of Medicine200065 ShanghaiChina Department of cardio-Pulmonary circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People’s Hospital affiliated to Tongji University School of Medicine200434 ShanghaiChina

Dear Editor,Major depressive disorder(MDD)is one of the most common psychiatric illnesses that significantly increase the risk of suicide.1 Stress-triggered dysfunctions of microglia have been identified as a commonly occurred pathological feature of MDD.1–3 Microglial dysfunction contributes to the pathogenesis of MDD via immunoresponses/neuroinflammation-mediated neural damage and pathological synapse loss-mediated neural circuit disruption.1 Although the involvement of microglia in MDD has been widely investigated,the molecular mechanisms underlying microglial dysfunction remain largely ***,we identified glutaminase 1(Gls1)as one key protein that participates in microglial dysfunction.4–6 Gls1 catalyzes the hydrolysis of glutamine to produce glutamate in the brain.4 Besides its well-known role in excitatory neurotoxicity,we found Gls1 up-regulation in microglia in animal models of Alzheimer’s disease and ischemic stroke.4,7 Gls1 activates microglia to overproduce cytokines and release inflammatory extracellular vesicles,therefore leading to neuroinflammation in animal models of Alzheimer’s disease and ischemic stroke.4–6 More importantly,Gls1 has been found to be up-regulated in the brains of MDD patients,and microglial Gls1 deficiency mitigated LPS-induced depression-like behaviors.8 However,LPS exposure is not an appropriate model to mimic MDD phenotypes,leaving the involvement of Gls1 in MDD an undetermined question.

关键词： involvement inflammation media