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Spatiotemporal microRNA profile in peripheral nerve regeneration:miR-138 targets vimentin and inhibits Schwann cell migration and proliferation

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Neural Regeneration Research 2018年第7期13卷 1253-1262页

作者： Travis B.Sullivan Litchfield C.Robert Patrick A.Teebagy Shannon E.Morgan Evan W.Beatty bryan j.Cicuto Peter K.Nowd Kimberly M.Rieger-Christ David j.bryan Department of Translational Research Lahey Hospital & Medical Center Tissue Engineering Laboratory Lahey Hospital & Medical Center Department of Plastic and Reconstructive Surgery Lahey Hospital & Medical Center

While the peripheral nervous system has regenerative ability,restoration of sufficient function remains a *** has been shown to be localized in axonal growth fronts and associated with nerve regeneration,including myelination,neuroplasticity,kinase signaling in nerve axoplasm,and cell migration;however,the mechanisms regulating its expression within Schwann cell（SC） remain *** aim of this study was to profile the spatial and temporal expression profile of micro RNA（mi RNA） in a regenerating rat sciatic nerve after transection,and explore the potential role of mi R-138-5 p targeting vimentin in SC proliferation and migration.A rat sciatic nerve transection model,utilizing a polyethylene nerve guide,was used to investigate mi RNA expression at 7,14,30,60,and 90 days during nerve *** levels of mi RNA expression were determined using microarray analysis and subsequently validated with quantitative real-time polymerase chain *** vitro assays were conducted with cultured Schwann cells transfected with mi RNA mimics and assessed for migratory and proliferative *** top seven dysregulated mi RNAs reported in this study have been implicated in cell migration elsewhere,and GO and KEGG analyses predicted activities essential to wound *** of one of these,mi RNA-138-5 p,into SCs reduced cell migration and *** R-138-5 p has been shown to directly target vimentin in cancer cells,and the luciferase assay performed here in rat Schwann cells confirmed *** results detail a role of mi R-138-5 p in rat peripheral nerve regeneration and expand on reports of it as an important regulator in the peripheral nervous system.

关键词： non-coding RNA neural regeneration nerve guide sciatic nerve transection peripheral nerve injury wound healing Gene Ontology processes Kyoto Encyclopedia of Genes and Genomes pathways microarray luciferase assay

Synthesis and Structures of A Series of Novel Rare Earth Transition Metal Sulfates

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journal of Rare Earths 2006年第6期24卷 659-662页

作者：王希渠刘鲁美 Allan j.jacobson Department of Chemistry and Texas Center for Superconductivity University of Houston Houston TX 77204-5003USA

Seven new rare earth transition metal sulfates were synthesized by hydrothermal reactions under conditions slightly above the critical point of water. Their crystal structures were determined from single crystal X-ray data. The compositions of the new compounds can be represented by two general formulae ： REM （OH） 3 （SO4） and RE2M （OH） 3 （SO4） 2F （H2O） with RE = Gd, Tb, Dy ; M = Ni, Cu. Three different crystal structure types were found for the formula REM （OH） 3 （SO4）. The structures of the new compounds all feature infinite chains of REOn coordination polyhedra, which are connected to chains of CuO6 or NiO6 octabedra. The limited size range of the rare earth cations observed in these compounds is most likely because of interactions between the octabedral chains and the chains of REOn polyhedra. The new compounds are closely related to the known yttrium transition metal sulfates.

关键词： rare earth sulfate transition metal sulfate hydrothermal synthesis crystal structure

Evaluation of natural goethite on the removal of arsenate and selenite from water

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journal of Environmental Sciences 2019年第2期31卷 133-141页

作者： Andrew T.jacobson Maohong Fan Department of Chemical Engineering University of Wyoming

Elevated arsenic and selenium concentrations in water cause health problems to both humans and wildlife. Natural and anthropogenic activities have caused contamination of these elements in waters worldwide, making the development of efficient cost-effective methods in their removal essential. In this work, removal of arsenate and selenite from water by adsorption onto a natural goethite(α-FeO OH) sample was studied at varying conditions. The data was then compared with other arsenate, selenite/goethite adsorption systems as much of literature shows discrepancies due to varying adsorption conditions. Characterization of the goethite was completed using inductively coupled plasma mass spectrometry, X-ray diffraction, Fouriertransform infrared spectroscopy, scanning electron microscopy, and Brunauer–Emmett–Teller surface area analysis. Pseudo-first order(PFO) and pseudo-second order(PSO) kinetic models were applied; including comparisons of different regression methods. Various adsorption isotherm models were applied to determine the best fitting model and to compare adsorption capacitates with other works. Desorption/leaching of arsenate and selenite was studied though the addition of phosphate and hydroxyl ions. Langmuir isotherm modeling resulted in maximum adsorption capacities of 6.204 and 7.740 mg/g for arsenate and selenite adsorption,respectively. The PSO model applied with a non-linear regression resulted in the best kinetic fits for both adsorption and desorption of arsenate and selenite. Adsorption decreased with increasing pH. Phosphate induced desorption resulted in the highest percentage of arsenate and selenite desorbed, while hydroxide induced resulted in the fastest desorption kinetics.

关键词： Arsenic Selenium Adsorption Desorption FeOOH Water treatment

The role of cysteine-rich secretory proteins in male fertility

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Asian journal of Andrology 2011年第1期13卷 111-117页

作者： Adam j Koppers Thulasimala Reddy Moira K O＇bryan Department of Anatomy and Developmental Biology School of Biomedical Sciences Monash University Clayton Vic. 3800 Australia

The cysteine-rich secretory proteins （CRISPs） are a subgroup of the CRISP, antigen 5 and Pr-1 （CAP） protein superfamily, and are found only in vertebrates. They show a strong expression bias to the mammalian male reproductive tract and the venom of poisonous reptiles. Within the male reproductive tract CRISPs have been implicated in many aspects of male germ cell biology spanning haploid germ cell development, epididymal maturation, capacitation, motility and the actual processes of fertilization. At a structural level, CRISPs are composed of two domains, a CAP domain, which has been implicated in cell-cell adhesion, and a CRISP domain, which has been shown to regulate several classes of ion channels across multiple species. Herein, we will review the current literature on the role of CRISPs in male fertility, and by inference to related non-mammalian protein, infer potential biochemical functions.

关键词： CRISPs epididymis fertility spermatozoa testis

Intestinal stem cells and the colorectal cancer microenvironment

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World journal of Gastroenterology 2014年第8期20卷 1898-1909页

作者： bryan A Ong Kenneth j Vega Courtney W Houchen Department of Digestive Diseases University of Oklahoma Health Sciences CenterOklahoma CityOK 73104United States

Colorectal cancer (CRC) remains a highly fatal condition in part due to its resilience to treatment and its propensity to spread beyond the site of primary occurrence. One possible avenue for cancer to escape eradication is via stem-like cancer cells that, through phenotypic heterogeneity, are more resilient than other tumor constituents and are key contributors to cancer growth and metastasis. These proliferative tumor cells are theorized to possess many properties akin to normal intestinal stem cells. Not only do these CRC “stem” cells demonstrate similar restorative ability, they also share many cell pathways and surface markers in common, as well as respond to the same key niche stimuli. With the improvement of techniques for epithelial stem cell identification, our understanding of CRC behavior is also evolving. Emerging evidence about cellular plasticity and epithelial mesenchymal transition are shedding light onto metastatic CRC processes and are also challenging fundamental concepts about unidirectional epithelial proliferation. This review aims to reappraise evidence supporting the existence and behavior of CRC stem cells, their relationship to normal stem cells, and their possible dependence on the stem cell niche.

关键词： Colon cancer stem cells DCLK1 protein Stem cell niche Cell dedifferentiation

Towards personalised public warnings: harnessing technological advancements to promote better individual decision-making in the face of disasters

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International journal of Digital Earth 2017年第12期10卷 1231-1252页

作者： Yinghui Cao bryan j.Boruff Ilona M.McNeill School of Earth and Environment The University of Western AustraliaCrawleyWAAustralia Melbourne School of Psychological Sciences The University of MelbourneParkvilleVICAustralia

Official warnings are essential for informing the public of impending hazards and promoting their responses before a disaster ***,research has identified that traditional public warnings,such as generic text messages based on large geographic regions,often fail to promote appropriate responses by at-risk ***,there has been an increased focus on using map-based approaches for communicating public ***,a systematic framework to guide the design of effective mapping instruments for this purpose is *** researchers sought to fill this gap by merging the scholarly understanding of factors influencing warning effectiveness with the contemporary spatial capacities of the emergency management *** current paper presents the conceptual framework resulting from this merger,which can be used to direct the design and implementation of map-based warnings that offer personalised risk visualisation and provide personalised decision support to motivate appropriate *** example is then provided to illustrate how this framework can be applied for the development of personalised bushfire warnings in an Australian *** by webGIS technologies,the proposed framework shows a potentially ground-breaking approach to improve public warning communication by fostering more efficient and effective risk personalisation and response related decision-making by individuals.

关键词： Public warning risk communication map GIS wildfire

加拿大Algoma公司新建的2^#无缝钢管机组

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湖南冶金 1989年第2期17卷 56-59页

作者： j.bryan Code 李长穆

Algonla钢公司新建一套无缝钢管机组是1979年开始筹划的,其目的是将石油管的直径范围下限扩大到48.3毫米(1.9英寸)。该公司原有的1~#机组虽然可以生产小到114亳米的钢管,但它破适宜的产品直径是177.8～324毫米。新建的这套机组将生产... 详细信息

Algonla钢公司新建一套无缝钢管机组是1979年开始筹划的,其目的是将石油管的直径范围下限扩大到48.3毫米(1.9英寸)。该公司原有的1~#机组虽然可以生产小到114亳米的钢管,但它破适宜的产品直径是177.8～324毫米。新建的这套机组将生产尺寸范围为48.3～177.8毫米的产品。

关键词：加拿大 Algoma公司无缝钢管机组

The effect of EMG magnitude on the masseter vestibular evoked myogenic potential(mVEMP)

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journal of Otology 2022年第4期17卷 203-210页

作者： Daniel j.Romero Gary P.jacobson Richard A.Roberts Department of Hearing and Speech Sciences Division of Vestibular SciencesVanderbilt University Medical Center121521st Ave.South6308 Medical Center EastNashvilleTN37232-8718USA

Introduction:The masseter vestibular evoked myogenic potential(mVEMP)is a bilaterally generated,electromyographically(EMG)-mediated response innervated by the trigeminal *** purpose of the present investigation was to 1)determine whether subjects could accurately achieve and maintain a range of EMG target levels,2)to examine the effects of varied EMG levels on the latencies and amplitudes of the mVEMP,and 3)to investigate the degree of side-to-side asymmetry and any effects of EMG ***:Subjects were nine neurologically and otologically normal young adults.A high-intensity tone burst was presented monaurally while subjects were seated upright and asked to match a range of EMG target levels by clenching their *** were made from the ipsilateral and contralateral masseter muscles referenced to the ear being monaurally ***:We found that the tonic EMG target had no effect on mVEMP ***,although mVEMP amplitudes“scaled”to the EMG target,there was a tendency for the subjects’EMG level to“undershoot”the EMG target levels greater than 50 *** some individuals did generate differences in EMG activation between sides,there were no significant differences on average EMG activation between ***,while average corrected amplitude asymmetry was similar across EMG targets,some individuals demonstrated large,corrected amplitude asymmetry ***:The results of this investigation suggest that,as with cVEMP recordings,the underlying EMG activation may vary between subjects and could impact mVEMP amplitudes,yet could be mitigated by amplitude correction *** it is important to be aware that even young normal subjects have difficulty maintaining large,tonic EMG activity during the mVEMP recording.

关键词： Vestibular evoked myogenic potentials (VEMPs) Masseter VEMPs Electromyography(EMG) Vestibulomasseteric reflex(VMR) EMG target Level

Characterization and evolutionary dynamics of complex regions in eukaryotic genomes

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Science China(Life Sciences) 2019年第4期62卷 467-488页

作者： josé Ranz bryan Clifton Department of Ecology and Evolutionary Biology University of California Irvine

Complex regions in eukaryotic genomes are typically characterized by duplications of chromosomal stretches that often include one or more genes repeated in a tandem array or in relatively close proximity. Nevertheless, the repetitive nature of these regions,together with the often high sequence identity among repeats, have made complex regions particularly recalcitrant to proper molecular characterization, often being misassembled or completely absent in genome assemblies. This limitation has prevented accurate functional and evolutionary analyses of these regions. This is becoming increasingly relevant as evidence continues to support a central role for complex genomic regions in explaining human disease, developmental innovations, and ecological adaptations across phyla. With the advent of long-read sequencing technologies and suitable assemblers, the development of algorithms that can accommodate sample heterozygosity, and the adoption of a pangenomic-like view of these regions, accurate reconstructions of complex regions are now within reach. These reconstructions will finally allow for accurate functional and evolutionary studies of complex genomic regions, underlying the generation of genotype-phenotype maps of unprecedented resolution.

关键词： complex genomic regions sequencing technologies genome assembly structural variation tandem gene duplicates,evolutionary novelty