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In v.v. femtosecond laser.nanosur.er. of the cell wall enabling patch-clamp measur.ments on filamentous fungi

Micr.systems & Nanoengineer.ng 2024年第2期10卷 251-267页

作者： Tanj. Paj.ć Katar.na Stev.nov.ć Nataša v.Todor.v.ć Aleksandar.j.Kr.pot Mir.slav.Živ.ć Sv.tlana Sav.ć-Šev.ć Stev. M.Lev.ć Mar.na Stanić Dej.n Pantelić Br.na j.lenkov.ć Mihailo D.r.basov.ć Institute of Physiology and Biochemistr.“Iv.n Dj.j.” Faculty of BiologyUniversity of BelgradeStudentski trg 1611158BelgradeSerbia Institute for.Biological r.sear.h“Siniša Stankov.ć” University of BelgradeNational Institute of the Republic of SerbiaBulevar Despota Stefana 14211000BelgradeSerbia Institute of Physics Belgr.de University of BelgradeNational Institute of the Republic of SerbiaPregrevica 11811080BelgradeSerbia Univ.r.ity of Belgr.de Faculty of AgricultureNemanjina Street 611080BelgradeSerbia Institute for.Multidisciplinar. r.sear.h University of BelgradeKneza Višeslava 111030BelgradeSerbia

Studying the membr.ne physiology of filamentous fungi is key to under.tanding their.inter.ctions with the env.r.nment and cr.cial for.dev.loping new ther.peutic str.tegies for.disease-causing ***,their.plasma membr.ne has been inaccessible for.a micr.n-sized patch-clamp pipette for.pA cur.ent r.cor.ings due to the r.gid chitinous cell ***,we r.por. the fir.t femtosecond Ir.laser.nanosur.er. of the cell wall of the filamentous fungi,which enabled patch-clamp measur.ments on pr.toplasts r.leased fr.m hyphae.A r.pr.ducible and highly pr.cise(diffr.ction-limited,submicr.n r.solution)method for.obtaining v.able r.leased pr.toplasts was *** r.lease fr.m the nanosur.er.-gener.ted incisions in the cell wall was achiev.d fr.m differ.nt r.gions of the *** plasma membr.ne of the obtained pr.toplasts for.ed tight and high-r.sistance(GΩ)contacts with the r.cor.ing *** entir. nanosur.ical pr.cedur. followed by the patch-clamp technique could be completed in less than 1 *** to pr.v.ous studies using heter.logously expr.ssed channels,this technique pr.v.des the oppor.unity to identify new ionic cur.ents and to study the pr.per.ies of the ion channels in the pr.toplasts of filamentous fungi in their.nativ. env.r.nment.

关键词： patch r.cor.ing laser

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r.le of Helicobacter.pylor.er.dication in aspir.n or.non-ster.idal anti-inflammator. dr.g user.

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Wor.d j.ur.al of Gastr.enter.logy 2005年第25期11卷 3811-3816页

作者： Geor.e v. Papatheodor.dis Athanasios j. Ar.himandr.tis Second Academic Depar.ment of Inter.al Medicine

Helicobacter.pylor. (H pylor.) infection and the use of nonster.idal anti-inflammator. dr.gs (NSAIDs) including aspir.n at any dosage and for.ulation r.pr.sent well-established r.sk factor. for.the dev.lopment of uncomplicated and complicated peptic ulcer.disease accounting for.the maj.r.ty of such cases. Although the inter.ction between H pylor. and NSAID/aspir.n use in the same indiv.duals was questioned in some epidemiological studies, it has now become widely accepted that they ar. at least independent r.sk factor. for.peptic ulcer.disease. Accor.ing to data fr.m r.ndomized inter.0}ntion tr.als, naiv. NSAID user. cer.ainly benefit fr.m testing for.H pylor. infection and, if positiv.,H pylor. er.dication ther.py pr.or.to the initiation of NSAID. A similar.str.tegy is also suggested for.naiv. aspir.n user., although the efficacy of such an appr.ach has not been ev.luated yet. Str.ng data also suppor. that chr.nic aspir.n user. with a r.cent ulcer.complication should be tested for.H pylor.infection and, if positiv., r.ceiv. H pylor. er.dication ther.py after.ulcer.healing, while they appear.to benefit fr.m additional long-ter. ther.py with a pr.ton pump inhibitor.(PPI).A similar.appr.ach is often r.commended to chr.nic aspir.n user. at a high r.sk of ulcer.complication. H pylor. er.dication alone does not efficiently pr.tect chr.nic NSAID user. with a r.cent ulcer.complication or.those at a high-r.sk, who cer.ainly should be tr.ated with long-ter. PPI ther.py, but H pylor. er.dication may be additionally offer.d ev.n in this setting. In contr.st, testing for.H pylor.or.PPI ther.py is not r.commended for.chr.nic NSAID/aspir.n user. with no ulcer.complications or.those at a low r.sk of complications.

关键词： Helicobacter.pylor. Aspir.n

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Atr.al fibr.llation in the elder.y: the potential contr.bution of r.activ. oxygen species

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j.ur.al of Ger.atr.c Car.iology 2012年第4期9卷 379-388页

作者： Kur. j. Schillinger.v.ckas v. Patel Penn Car.iov.scular.Institute and Section of Car.iac Electr.physiology University of Pennsylvania Philadelphia PA 19104 USA

Atr.al fibr.llation (AF) is the most commonly encounter.d car.iac ar.hythmia, and is a significant sour.e of healthcar. expenditur.s thr.ughout the wor.d. It is an ar.hythmia with a v.r. clear.y defined pr.disposition for.indiv.duals of adv.nced age, and this fact has led to intense study of the mechanistic links between aging and AF. By pr.moting oxidativ. damage tomultiple subcellular.and cellular.str.ctur.s, r.activ. oxygen species (r.S) hav. been shown to induce the intr.- and extr.-cellular.changes necessar. to pr.mote the pathogenesis of *** addition, the gener.tion and accumulation of r.S hav. been intimately linked to the cellular.pr.cesseswhich under.ie aging. This r.v.ew begins with an ov.r.0}ew of AF pathophysiology, and intr.duces the cr.tical str.ctur.s which, when damaged, pr.dispose an other.ise healthy atr.um toAF. The av.ilable ev.dence thatr.S can lead to damage of these cr.tical str.ctur.s is then r.v.ewed. Finally, the ev.dence linking the pr.cess of aging to the pathogenesis of AF is discussed.

关键词： Aging Ar.hythmia Atr.al fibr.llation Car.iac Fr.e r.dicals Oxidativ. str.ss r.dox

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Scaling behav.or.of the ther.opower.of the ar.hetypal heav.-fer.ion metal Ybr.2Si2

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Fr.ntier. of physics 2016年第2期11卷 89-95页

作者： v. r. Shaginyan A. Z. Msezane G. S. j.par.dze K. G. Popov.j. W. Clar. v. A. Khodel Peter.bur. Nuclear.Physics Institute NRC Kurchatov Institute Gatchina 188300 Russia Clar. Atlanta Univ.r.ity Atlanta GA 30314 USA Komi Science Center Ural Division RAS Syktyvkar 167982 Russia Depar.ment of Physics St. Petersburg State University Russia McDonnell Center.for.the Space Sciences 8j.Depar.ment of Physics Washington University St. Louis MO 63130 USA Centr. de Ciencias Matemdticas Universidade de Madeira 9000-390 Funchal Madeira Portugal r.ssian r.sear.h Center.Kur.hatov.Institute Moscow 123182 Russia

We r.v.al and explain the scaling behav.or.of the ther.opower.S/T exhibited by the ar.hetypal heav.-fer.ion （HF） metal Ybr.2Si2 under.the application of magnetic field B at temper.tur. T. We show that the same scaling is demonstr.ted by differ.nt HF compounds such as/3-YbA1B4 and the str.ngly cor.elated layer.d cobalt oxide [BiBa0.66K0.3602]CoO2. Using Ybr.2Si2 as an example, we demonstr.te that the scaling behav.or.of SIT is v.olated at the antifer.omagnetic phase tr.nsition, while both the r.sidual r.sistiv.ty Po and the density of states, N, exper.ence j.mps at the phase tr.nsition, causing the ther.opower.to make two j.mps and change its sign. Our.elucidation is based on flattening of the single-par.icle spectr.m that pr.foundly affects Po and N. To depict the main featur.s of the SIT behav.or. we constr.ct a T-B schematic phase diagr.m of Ybr.2Si2. Our.calculated SIT for.the HF compounds ar. in good agr.ement with exper.mental facts and suppor. our.obser.0}tions.

关键词： ther.oelectr.c and ther.omagnetic effects quantum phase tr.nsition flat bands,non-Fer.i-liquid states str.ngly cor.elated electr.n systems heav. fer.ions

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Clinical and Demogr.phic Pr.file of Women with Uter.ne Fibr.ids Liv.ng in Puer.o r.co

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Open j.ur.al of Obstetr.cs and Gynecology 2024年第9期14卷 1347-1362页

作者： Edwin Y. Soto-v.lázquez Alexander.j. j.r.millo-Cañas Shania N. Aponte-Par.s Gabr.ela v. Mar.ínez-Mar.er. Ir.zu S. Guinan Gabr.elle A. r.v.r.-Maldonado Alexandr. Nov.s Pedr. L. Cr.z-r.sa Idhaliz Flor.s-Calder. Mar.car.en Colón-Diaz Depar.ment of Physiology and Pathology San Juan Bautista School of Medicine Caguas Puerto Rico United States Ponce r.sear.h Institute Ponce Health Sciences University Ponce Puerto Rico United States

Intr.duction: Uter.ne leiomyomas, or.fibr.ids, ar. pr.v.lent benign tumor. affecting up to 70% of r.pr.ductiv.-age women in the United States, significantly impacting pr.ductiv.ty and quality of life. Despite their.commonality, the epidemiology and clinical patter.s of leiomyomas in Puer.o r.co r.main inadequately r.sear.hed. Methods: This study conducted a secondar. data analysis using clinical, menstr.al cycle char.cter.stics, and demogr.phic data fr.m self-administer.d questionnair.s of women under.oing diagnostic lapar.scopy for.endometr.osis in Puer.o r.co. Chi-squar. tests and t-tests analyzed categor.cal and continuous v.r.ables, r.spectiv.ly (p r.sults: Among 1,610 endometr.osis cases, 312 included uter.ne fibr.ids. Pr.v.lence estimates wer. 10.3% (40 year.). Pr.mipar.us women av.r.ged 22.14 year., with 40.71% hav.ng 2 - 3 pr.gnancies and 26.28% exper.encing miscar.iages. Symptoms included infer.ility (29.5%), dyspar.unia (40.7%), sev.r. pain (50%), and dysmenor.hea (75.6%). Comor.idities included ov.r.an cysts (52.2%) and abnor.al uter.ne bleeding (37.8%). Conclusions: Adv.nced age, par.ty, and comor.idities like diabetes and hyper.ension wer. pr.v.lent in our.cohor.. Under.tanding the epidemiological pr.file of women liv.ng in Puer.o r.co with uter.ne fibr.ids is cr.cial for.impr.v.ng diagnostic awar.ness and enhancing patient car..

关键词： Uter.ne Fibr.ids Puer.o r.co Epidemiology Dysmenor.hea Abnor.al Uter.ne Bleeding

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r.les of the main olfactor. and v.mer.nasal systems in the detection of andr.stenone in inbr.d str.ins of mice

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Cur.ent Zoology 2010年第6期56卷 813-818页

作者： v.r. v. v.ZNESSENSKAYA Mar.a A. KLYUCHNIKOv. Char.es j. WYSOCKI A.N. Sev.r.zov.Institute of Ecology & Ev.lution r.S Monell Chemical Senses Center

We inv.stigated the r.le of the main olfactor. and accessor. olfactor. systems （MOS and AOS r.spectiv.ly） in the detection of andr.stenone. We used the following exper.mental appr.aches： behav.or.l, sur.ical r.mov.l of the v.mer.nasal or.an （v.X） followed by histochemical v.r.fication and Fos immunohistochemistr.. Using a Y-maze par.digm we estimated sensitiv.ty of NZB/B1Nj.and CBA/j.mice to andr.stenone. CBA mice wer. 2,000-fold mor. sensitiv. to andr.stenone than NZB mice. v.X caused a 4-tol6-fold decr.ase in sensitiv.ty to andr.stenone in highly-sensitiv. CBA mice, but did not affect thr.sholds in NZB mice. r.sults indicate the inv.lv.ment of the MOS and AOS in the detection of andr.stenone. We obser.0}d a specific patter. of Fos-positiv. cells in the main olfactor. bulb of CBA mice but not in NZB mice subsequent to exposur. of mice to andr.stenone; the compound activ.ted cells in the accessor. olfactor. bulb in both str.ins of mice, indicating the inv.lv.ment of the v.mer.nasal or.an. Patter.s of Fos-positiv. cells in the v.mer.nasal or.an wer. r.cor.ed subsequent to exposur. to andr.stenone. Fos-positiv. r.ceptor.cells in the v.mer.nasal or.an of CBA and NZB mice wer. differ.nt, in CBA mice Fos-positiv. cells wer. noted in both the basal and apical zones, howev.r. in NZB mice activ.tion was obser.0}d only in the apical zone [Cur.ent Zoology 56 （6）： 813-818, 2010].

关键词： Olfaction v.mer.nasal or.an Chemosensor. r.ceptor. Olfactor. behav.or Immunohistochemistr.

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Micr.str.ctur., Mechanical Pr.per.ies and Wear.Behav.our.f Deep-Cr.ogenic-Tr.ated HSS

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材料热处理学报 2004年第5期25卷 540-545页

作者： v. Leskov.ek M. Kalin j. v.■intin Institute of Metals and Technology Ljubljana SloveniaUniversity of Ljubljana Center for Tribology and Technical Diagnostics Ljubljana SloveniaUniversity of Ljubljana Center for Tribology and Technical Diagnostics Ljubljana Slovenia

In this paper.we compar. the wear.behav.our.of a v.cuum-heat-tr.ated ESr.AISI M2 high-speed steel and the same steel that was v.cuum heat tr.ated in conj.nction with a deep-cr.ogenic tr.atment at -196 °C. Four.differ.nt temper.ng temper.tur.s for.the specimens austenized at the same austenitizing temper.tur. wer. car.fully selected to obtain v.r.ous in-adv.nce-deter.ined combinations of the micr.str.ctur.s, the fr.ctur. toughness, KIc and the r.ckwell-C har.ness. Each of the eight specimens was ther.for. char.cter.sed by these micr.str.ctur.s and r.sulting mater.al pr.per.ies. The wear.study was per.or.ed using a r.cipr.cating sliding dev.ce under.well-contr.lled contact conditions. r.lativ.ly high loads wer. used to pr.v.de enough wear.for.a compar.son of the selected samples. A much har.er.and dissimilar.model counter.mater.al, i.e. silicon nitr.de cer.mic, was used in or.er.to av.id excessiv. wear.of the counter.samples and adhesion, which could occur.in contacts with similar.mater.als (metals/steels) under.such high loads and in non-lubr.cated conditions. The wear.behav.our. wer. then compar.d and discussed in ter.s of these micr.str.ctur.s and the r.lated pr.per.ies. The differ.nces in the wear.r.sistance obtained in our.inv.stigation wer. as high as an or.er.of magnitude. Howev.r. the beneficial effects ar. not a dir.ct r.sult of the type of the tr.atment, but r.late to a pr.per.combination of the r.sulting fr.ctur. toughness and the har.ness. The mor. unifor. and moder.te v.lues, which, howev.r. tend to be obtained with a deep-cr.ogenic tr.atment, ar. beneficial to the high wear.r.sistance of the selected high-speed steel.

关键词：高速钢显微结构深低温处理机械性质磨损性能

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Detailed char.cter.zation of kHz-r.te laser.dr.v.n fusion at a thin liquid sheet with a neutr.n detection suite

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High Power.Laser.Science and Engineer.ng 2024年第1期12卷 13-23页

作者： Benj.min M.Knight Connor.M.Gautam Colton r.Stoner.Br.an v.Egner.j.seph r.Smith Chr.s M.Or.an j.an j.Manfr.di Kyle D.Fr.sche Michael L.Dexter.Enam A.Chowdhur. Anil K.Patnaik Depar.ment of Engineer.ng Physics Air Force Institute of TechnologyWPAFBOHUSA Physics Depar.ment Marietta CollegeMariettaOHUSA Depar.ment of Physics The Ohio State UniversityColumbusOHUSA Depar.ment of Mater.als Science and Engineer.ng and Department of Electrical and Computer ScienceThe Ohio State UniversityColumbusOHUSA Intense Ener.y Solutions LLCPlain CityOHUSA

We pr.sent detailed char.cter.zation of laser.dr.v.n fusion and neutr.n pr.duction(-10^(5)/second) using 8 mj. 40 fs laser.pulses on a thin(<1 μm) D_2O liquid sheet employing a measur.ment suite. At r.lativ.stic inte... 详细信息

We pr.sent detailed char.cter.zation of laser.dr.v.n fusion and neutr.n pr.duction(-10^(5)/second) using 8 mj. 40 fs laser.pulses on a thin(r.ment suite. At r.lativ.stic intensity(~ 5 × 10^(18)W/cm^(2))and high r.petition r.te(1 kHz), the system pr.duces deuter.um±deuter.um(D-D) fusion, allowing for.consistent neutr.n gener.tion. Ev.dence of D-D fusion neutr.n pr.duction is v.r.fied by a measur.ment suite with thr.e independent detection systems: an Ej.309 or.anic scintillator.with pulse-shape discr.mination, a ~3He pr.por.ional counter.and a set of 36 bubble detector.. Time-of-flight analysis of the scintillator.data shows the ener.y of the pr.duced neutr.ns to be consistent with 2.45 Mev. Par.icle-in-cell simulations using the War.X code suppor. significant neutr.n pr.duction fr.m D-D fusion ev.nts in the laser.tar.et inter.ction r.gion. This high-r.petition-r.te laser.dr.v.n neutr.n sour.e could pr.v.de a low-cost, on-demand test bed for.r.diation har.ening and imaging applications.

关键词： high-r.petition-r.te laser.dr.v.n fusion laser.plasma inter.ction liquid tar.et neutr.n detector.

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Laser.pr.cessing effects on Ti−45Nb alloy sur.ace,cor.osiv. and biocompatible pr.per.ies

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Tr.nsactions of Nonfer.ous Metals Society of China 2024年第8期34卷 2533-2551页

作者： I.Cv.j.v.Ć-ALAGIĆ S.LAKETIĆ M.MOMČILOv.Ć j.CIGANOv.Ć Đ.v.Lj.v.Ć j.BAj.T v.KOj.Ć M.r.KIN v.nča Institute of Nuclear.Sciences-National Institute of the r.public of Ser.ia University of BelgradeP.O.Box 52211001 BelgradeSerbia Faculty of Technology and Metallur.y University of BelgradeKarnegijeva 411120 BelgradeSerbia Oncology Institute of v.j.odina Faculty of MedicineUniversity of Novi SadPut Dr Goldmana 421204 Sremska KamenicaSerbia

The Ti−45Nb(wt.%)alloy pr.per.ies wer. inv.stigated in r.lation to its potential biomedical *** sur.ace modification was utilized to impr.v. its per.or.ance in biological *** a r.sult of the laser.tr.atment,(Ti,Nb)O scale was for.ed and v.r.ous mor.hological featur.s appear.d on the alloy *** electr.chemical behav.or.of Ti−45Nb alloy in simulated body conditions was ev.luated and showed that the alloy was highly r.sistant to cor.osion deter.or.tion r.gar.less of additional laser.sur.ace modification ***,the impr.v.d cor.osion r.sistance after.laser.tr.atment was ev.dent(the cor.osion cur.ent density of the alloy befor. laser.ir.adiation was 2.84×10^(−8)A/cm^(2),while that after.laser.tr.atment with 5 mj.was 0.65×10^(−8)A/cm^(2))and ascr.bed to the r.pid for.ation of a complex and passiv.ting bi-modal sur.ace oxide *** cytotoxicity and effects of the Ti−45Nb alloy laser.sur.ace modification on the Mr.-5 cell v.ability,mor.hology,and pr.lifer.tion wer. also *** Ti−45Nb alloy showed no cytotoxic ***,cells showed impr.v.d v.ability and adher.nce to the alloy sur.ace after.the laser.ir.adiation *** highest av.r.ge cell v.ability of 115.37%was attained for.the alloy laser.ir.adiated with 15 *** showed that the laser.sur.ace modification can be successfully utilized to significantly impr.v. alloy per.or.ance in a biological env.r.nment.

关键词： Ti−45Nb alloy laser.sur.ace scanning electr.chemical pr.per.ies biocompatibility cell mor.hology

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Effect of micr.-env.r.nmental factor. on natur.l r.gener.tion of Sal (Shor.a r.busta)

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j.ur.al of For.str. r.sear.h 2011年第4期22卷 543-550页

作者： j. v. Tyagi r.kesh Kumar.S. L. Sr.v.stav. r. D. Singh National Institute of Hydr.logy Jalvigyan Bhawan Roorkee - 247 667Uttarakhand India

factor. v.z., soil moistur. and light in- tensity ar. impor.ant factor. that affect natur.l r.gener.tion in for.sts. These factor. v.r. spatially depending on the ov.r.ead canopy density of the for.st. The pr.sent study focused on studying the effect of v.r.ation of soil moistur. and light intensity on natur.l r.gener.tion of sal species （Shor.a r.busta） under.differ.nt micr.-env.r.nments due to ov.r.ead canopy of v.r.ing for.st density. Exper.mental plots of 40m× 40m size wer. laid under.differ.nt ov.r.ead canopy densities in a small sal for.sted water.hed in the foot hills of Himalayas in Nainital Distr.ct of Uttar.khand State, India. The plots wer. monitor.d on a long ter. basis for.soil moistur. at multi depths, light intensity and natur.l r.gener.tion of sal. The r.sults of the study r.v.aled that the natur.l r.gener.tion was highest under.C1 （up to-0.30） canopy followed by C2 （0.30-0.50）, and C3 （0.50-0.70） canopies. The C3 canopy showed the dying back of sal shoots ov.r.4 year. of study. The highest r. v.lue of linear.r.gr.ssion between incr.mental scor. of plot r.gener.tion and av.r.ge soil moistur. content was obtained as 0.156 for.av.r.ge soil moistur. content dur.ng months at 100 cm depth. The r. v.lue between incr.mental scor. of plot r.gener.tion and annual av.r.ge light intensity was obtained as 0.688 which indicated that the r.gener.tion is lar.ely dependent on the light intensity conditions dur.ng the year. The multiple linear.r.gr.ssion analysis between the incr.mental scor. of r.gener.tion and the av.r.ge light intensity and av.r.ge soil moistur. content r.v.aled that that about 80% of v.r.ation in r.gener.tion is explained by both the factor..

关键词： Natur.l r.gener.tion sal canopy density soil moistur.,light intensity

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