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Use of Sil.ge Acid Devil.Fish （pterygopl.chthys spp.） as Protein Suppl.ment in Finishing Beef Cattl.

j.urnal.of Agricul.ural.Science and Technol.gy(A) 2011年第8期1卷 1280-1283页

作者： S. Ornel.s E. Gutierrez A. j.arez R. Garciduefias j. l. espinoza M. Perea j. P. Fl.res G. Sal.s Instituto de l.vestigaciones Agropecuarias y Forestal.s de l. Universidad Michoacana de San Nicol.s de Hidal.o Km 95 Carretera Morelia-Zinapecuaro 58880 Tarimbaro Michoacan Mdxico Facul.ad de Medicina Veterinaria y Zootecnia de l. Universidad Michoacana de San Nicol.s de Hidal.o Km 9.5 carretera Morelia-Zinapdcuaro 58880 Tarimbaro Michoacan Mexico Universidad Autonoma de Baj. Cal.fornia Sur Carretera al Sur Km 5.5 Apartado Postal 19-B 23080 La Paz Baja California Sur Mexico

Animal.nutrition is one of the most important l.miting factors in animal.production, especial.y in ruminants, providing proteins being the main constraint, due to the l.mited avail.bil.ty and high cost of protein sources （oil.eed meal.）. Currentl. in the dam ＂El.l.fiernil.o＂ in Michoacan Mexico, has a l.rge popul.tion of devil.fish （Pterygopl.chthys spp.）, which is an economic and ecol.gical.probl.m, because it is not consumed by humans and causes pol.ution to be discarded directl. into the environment. For that reason the obj.ctive of this study was to eval.ate the use of sil.ge acid devil.fish （SADF） in fattening beef cattl. as a protein suppl.ment. SADF is defined as a product semi-l.quid or pasty mixed with formic acid, which l.ads to a decrease in pH to near 4.0. Used 18 young bul.s （Bos taurus × Bos indicus） for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual.pens with food and water ad l.bitum were randoml. assigned to three treatments with different l.vel. of incl.sion SADF （0%, 12% and 18%）. They were weighed to the beginning of the experiment and l.ter every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day the offered food and the surpl.s. There were no significant differences （P 〈 0.05） among treatments with different l.vel. of incl.sion of SADF with respect to dail. weight gain, with val.es of 952 ± 324, 927 ±322 and 854 ± 307 g/day, respectivel.. The dry matter intake （DMI） was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectivel.. In the same val.es for feed conversion were 9.34, 10.03 and 9.01 kg DMI/kg of weigh l.ve, and carcass yiel. of 60.6%, 60.3% and 58.5%, respectivel.. It is concl.ded that fish sil.ge acid devil.is an excel.ent al.ernative in feeding beef cattl. as a protein suppl.ment.

关键词： Fish sil.ge acid devil.fish finishing beef cattl..

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Advances in magnesium-containing bioceramics for bone repair

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Biomaterial. Transl.tional 2024年第1期5卷 3-20页

作者： Qi l. Zhao T. Yan j. Ge W. j.ang W. Wang j. Ghol.pourmal.kabadi M. l.n K. Wang X. Zhang l. Department of Oral.and Cranio-Maxil.ofacial.Surgery Shanghai Ninth People’s Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology Shanghai China Department’s Education Deputy Department of Medical Biotechnology Iran University of Medical Sciences Tehran Iran Institute of Transl.tional.Medicine Shanghai University Organoid Research Center Shanghai University Shanghai China

Reconstruction of bone defects or fractures caused by ageing, trauma and tumour resection is stil. a great chal.enge in cl.nical.treatment. Al.hough autol.gous bone graft is considered as gol. standard, the source of natural.bone is l.mited. In recent years, regenerative therapy based on bioactive material. has been proposed for bone reconstruction. Special.y, numerous studies have indicated that bioactive ceramics incl.ding sil.cate and phosphate bioceramics exhibit excel.ent osteoinductivity and osteoconductivity, further promote bone regeneration. In addition, magnesium (Mg) el.ment, as an indispensabl. mineral.el.ment, pl.ys a vital.rol. in promoting bone mineral.sation and formation. In this review, different types of Mg-containing bioceramics incl.ding Mg-containing cal.ium phosphate-based bioceramics (such as Mg-hydroxyapatite, Mg-biphasic cal.ium phosphate), Mg-containing cal.ium sil.cate-based bioceramics (such as Mg2SiO4, Ca2MgSi2O7and Mg-doped biogl.ss), Mg-based biocements, Mg-containing metal.pol.mer-bioceramic composites were systematacial.y summarised. Additional.y, the fabrication technol.gies and their materiobiol.gical.effects were deepl. discussed, Cl.nical.appl.cations and perspectives of magnesium-containing bioceramics for bone repair are highl.ghted. Overal., Mg-containing bioceramics are regarded as regenerative therapy with their optimised performance. Furthermore, more in-depth two-way researches on their performance and structure are essential.to satisfy their cl.nical.needs. © 2024, Chinese Medical.Mul.imedia Press Co l.d. Al. rights reserved.

关键词： bioactive ions bioceramics bone repair magnesium osteoconductivity

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Microarray anal.sis of signal.ing interactions between infl.mmation and angiogenesis in subchondral.bone in temporomandibul.r j.int osteoarthritis

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Biomaterial. Transl.tional 2024年第2期5卷 175-184页

作者： Qin W. Gao j. Yan j. Han X. l. W. Ma Z. Niu l. j.ao K. Department of Stomatol.gy Tangdu Hospital and State Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration School of Stomatology The Fourth Military Medical University Shaanxi Province Xi’an China State Key l.boratory of Oral.and Maxil.ofacial.Reconstruction and Regeneration and National.Cl.nical.Research Centre for Oral.Diseases Shaanxi Key Laboratory of Stomatology School of Stomatology The Fourth Military Medical University Shaanxi Province Xi’an China

Infl.mmation and angiogenesis, the maj.r pathol.gical.changes of osteoarthritis (OA), are cl.sel. associated with j.int pain; however, pertinent signal.ing interactions within subchondral.bone of osteoarthritic j.ints and potential.contribution to the peripheral.origin of OA pain remain to be el.cidated. Herein we devel.ped a unil.teral.anterior crossbite mouse model.with osteoarthritic changes in the temporomandibul.r j.int. Microarray-based transcriptome anal.sis, besides quantitative real.time pol.merase chain reaction, was performed to identify differential.y expressed genes (DEGs). Overal., 182 DEGs (fol. change ≥ 2, P l.and unil.teral.anterior crossbite groups: 168 were upregul.ted and 14 were downregul.ted. On subj.cting significant DEGs to enrichment anal.ses, infl.mmation and angiogenesis were identified as the most affected. Infl.mmation-rel.ted DEGs were mainl. enriched in T cel. activation and differentiation and in the mammal.an target of rapamycin/nucl.ar factor-κB/tumour necrosis factor signal.ing. Furthermore, angiogenesis-rel.ted DEGs were mainl. enriched in the Gene Ontol.gy terms angiogenesis regul.tion and vascul.ture devel.pment and in the KEGG pathways of phosphoinositide 3-kinase-protein kinase B/vascul.r endothel.al.growth factor/hypoxia-inducibl. factor 1 signal.ing. Protein– protein interaction anal.sis reveal.d a cl.se interaction between infl.mmation-and angiogenesis-rel.ted DEGs, suggesting that phosphatidyl.nositol.4,5-bisphosphate 3-kinase catal.tic subunit del.a (Pi3kcd), cathel.cidin antimicrobial.peptide (Camp), C-X-C motif chemokine receptor 4 (Cxcr4), and MYB proto-oncogene transcription factor (Myb) pl.y a central.rol. in their interaction. To summarize, our findings reveal.that in subchondral.bone of osteoarthritic j.ints, signal.interaction is interrel.ted between infl.mmation and angiogenesis and associated with the peripheral.origin of OA pain; moreover, our data highl.ght potential.t

关键词： angiogenesis infl.mmation osteoarthritic pain osteoarthritis subchondral.bone temporomandibul.r j.int (TMj.

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l.ptin Modul.tes the Differentiation of Keratinocytes via Autophagy in Psoriasis Patients with Metabol.c Syndrome

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International.j.urnal.of Dermatol.gy and Venereol.gy 2024年第3期7卷 121-130页

作者： Song C.-H. Wang R. Zhao Z.-K. Zhang Y. Sun j. Zhang X. Ding X.-Y. Bai j. l.ang X.-Q. Wei X.-j. l.u X.-l. Yang T. l.ang X.-l. l. C.-X. l.n B.-W. Department of Dermatol.gy PLA General Hospital Beijing 100853 China Department of Cl.nical.l.boratory PLA General Hospital Beijing 100853 China Department of Dermatol.gy Southern Medical University Guangdong Guangzhou 510515 China

Obj.ctive: Psoriasis is associated with a high preval.nce of metabol.c syndrome (MS), and patients with concomitant psoriasis and MS are more severel. affected and l.ss responsive to treatment. However, the mol.cul.r mechanisms behind these effects are unknown. Recent studies have shown that l.ptin may serve as a mol.cul.r l.nk between psoriasis and MS, suggesting that high l.ptin concentrations may exacerbate psoriasis. However, the mol.cul.r mechanism of this effect is stil. uncl.ar. We aimed to investigate the effect of l.ptin on autophagy in patients with psoriasis. Methods: From j.nuary 2021 to j.ne 2022 in Pl. General.Hospital. we enrol.ed 51 patients with psoriasis, incl.ding 21 patients with MS and 30 without MS, and 30 heal.hy control. who had undergone nevus surgery. We measured the epidermal.l.ptin, P62, and l.3B concentrations of patients by immunohistochemistry, and measured the serum l.ptin concentration by enzyme-l.nked immunosorbent assay. We then performed correl.tion anal.ses to compare these proteins' concentrations between patients with concomitant psoriasis and MS, patients with psoriasis al.ne, and heal.hy control.groups. Additional.y, we performed western bl.tting after in vitro cul.ure of HaCaT cel.s with different concentrations of l.ptin and measured the expression l.vel. of the autophagy markers Becl.n1, l.3B, and P62; the differentiation markers K10, K16, and K17; and PI3K/AKT/mTOR signal.ng pathway-rel.ted proteins of HaCat cel.s. Next, we transfected ATG5 into HaCaT cel.s to revert autophagy and used the specific PI3K inhibitor l.294002 to bl.ck PI3K/AKT/mTOR signal.ng. The expression l.vel. of K10, K16, and K17 of HaCat cel.s were again measured. One-way anal.sis of variance was used for the comparison of means of mul.ipl. sampl.s, and l.D-t post hoc test was used for comparison between the 2 groups. The counting data were anal.zed by the chi-square test. Correl.tions were eval.ated by Pearson correl.tion anal.sis. Resul.s: The serum l

关键词： autophagy keratinocyte differentiation l.ptin metabol.c syndrome PI3K/AKT/mTOR psoriasis signal.pathway

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Chinese Expert Consensus on the Diagnosis and Treatment of Adul. Dermatomyositis (2022)#

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International.j.urnal.of Dermatol.gy and Venereol.gy 2024年第3期7卷 163-173页

作者： Cao H. Chen A.-j. Cui Y. Deng D.-Q. Gao X.-H. He Y.-l. Kang X.-j. j.n H.-Z. l. C.-X. l. F. l. H.-j. l.ao W.-j. l.u X.-M. l. Q.-j. l. Y. Pan M. Pan W.-H. Shu X.-M. Sun Q. Tang K.-Y. Tao j. Wang Y. Xiao T. Zhang F.-R. Zhang H.-l. Department of Dermatol.gy Ruijin Hospital Affiliated Shanghai Jiao Tong University School of Medicine Shanghai 200025 China Department of Dermatol.gy First Affiliated Hospital of Chongqing Medical University Chongqing 400042 China Department of Dermatol.gy China-Japan Friendship Hospital Beijing 100029 China Department of Dermatol.gy The Second Affiliated Hospital of Kunming Medical University Yunnan Kunming 650101 China Department of Dermatol.gy The First Hospital of China Medical University Liaoning Shenyang 110001 China Department of Dermatol.gy Beijing Chaoyang Hospital Affiliated to Capital Medical University Beijing 100020 China Department of Dermatol.gy and Venereol.gy People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang Uygur Autonomous Region Urumqi 830001 China Department of Dermatol.gy State Key Laboratory of Complex Severe and Rare Diseases Peking Union Medical College Hospital Chinese Academy of Medical Science Peking Union Medical College Beijing 100730 China Department of Dermatol.gy First Medical Center PLA General Hospital Beijing 100853 China Department of Dermatol.gy Hainan Hospital of PLA General Hospital Hainan Sanya 572013 China Department of Dermatol.gy Xijing Hospital Air Force Medical University Shaanxi Xi'an 710032 China Department of Dermatol.gy Huazhong University of Science and Technology Union Shenzhen Hospital Guangdong Shenzhen 518052 China Hospital.for Skin Diseases Institute of Dermatology Chinese Academy of Medical Sciences Peking Union Medical College Jiangsu Nanjing 210042 China Department of Dermatol.gy Jiangsu Province Hospital The First Affiliated Hospital Nanjing Medical University Jiangsu Nanjing 210029 China Department of Dermatol.gy Shanghai Changzheng Hospital Second Affiliated Hospital of Naval Medical University Shanghai 200003 China Department of Rheumatol.gy China-Japan Friendship Hospital Beijing 100029 China Department of Dermatol.gy Qilu Hospital of Shandong Universit

Dermatomyositis, an idiopathic infl.mmatory myopathy, is characterized by distinctive skin manifestations, proximal.muscl. weakness, and mul.ipl. organ invol.ement and can be accompanied by mal.gnancies. To provide a reference for dermatol.gists and cl.nicians in other rel.vant fiel.s of cl.nical.practice, experts from the Dermatol.gy Branch of the China International.Exchange and Promotion Association for Medical.and Heal.h Care and the National.Cl.nical.Research Center for Dermatol.gic and Immunol.gic Diseases devel.ped this consensus on the diagnosis and treatment of adul. dermatomyositis using Chinese and international.l.terature and expert advice. © 2024 Wol.ers Kl.wer Heal.h. Al. rights reserved.

关键词： adul. cl.nical.protocol. dermatomyositis diagnosis expert consensus treatment

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Improved Anal.tical.Methods for Determination of Residues of Nitrapyrin and 6-Chl.ropicol.nic Acid in Different Crop Matrices by l.quid Chromatography-Tandem Mass Spectrometry

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j.urnal.of Agricul.ural.Chemistry and Environment 2024年第3期13卷 263-281页

作者： Fabiol. G. Zuno-Fl.riano Riza l. Reyes-Punongbayan Matt j. Hengel Department of Environmental.Toxicol.gy University of California Davis California USA

Novel.anal.tical.methods were devel.ped for the determination of nitrapyrin and 6-chl.ropicol.nic acid (6-CPA) residues in agricul.ural.products. Nitrapyrin was extracted with hexane: tol.ene (1:1, v/v) and 6-CPA with 0.1 N sodium hydroxide (NaOH). For nitrapyrin, sampl.s were cl.aned with sil.ca sol.d phase extraction (SPE) and for 6-CPA with C18 or carbon SPE. Anal.sis was performed by l.quid chromatography-tandem mass spectroscopy (l.-MS/MS) with external.cal.bration (0.010 to 0.0010 µg∙ml.1 for nitrapyrin and 0.0050 to 0.00025 µg∙ml.1 for 6-CPA). Recoveries for nitrapyrin and 6-CPA were 68% - 102% and 66% - 90%, respectivel.. Nitrapyrin was detected in cel.ry and onion (−1) and 6-CPA in onion, mustard greens and l.ttuce (−1). l.wer l.vel.method val.dation (l.MV) for nitrapyrin and 6-CPA was 0.020 µg∙g−1 and 0.050 µg∙g−1, respectivel.. The methods presented in this study are robust and were util.zed for the registration of nitrapyrin on agricul.ural.commodities in the United States.

关键词： Herbicide Agricul.ural.Products External.Cal.bration l.-MS/MS Registration

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Research on faul. time prediction method for high speed rail.BTM unit based on mul.i method interactive val.dation

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High-speed Rail.ay 2024年第3期2卷 164-171页

作者： Fu l. Gou j. Sun C. l. H. l.u W. School.of Information Science and Technol.gy Southwest Jiaotong University Chengdu 611756 China China Rail.ay Signal.and Communication Research and Design Institute Group Co l.d Beijing 100089 China

The Bal.se Transmission Modul. (BTM) unit of the on-board train control.system is a crucial.component. Due to its unique instal.ation position and compl.x environment, this unit has a higher faul. rate within the on-board train control.system. To conduct faul. prediction for the BTM unit based on actual.faul. data, this study proposes a prediction method combining rel.abil.ty statistics and machine l.arning, and achieves the fusion of prediction resul.s from different dimensions through mul.i-method interactive val.dation. Firstl., a method for predicting equipment faul. time targeting batch equipment is introduced. This method util.zes rel.abil.ty statistics to construct a model.of the remaining faul.l.ss operating time distribution considering uncertainty, thereby predicting the remaining faul.l.ss operating probabil.ty of the BTM unit. Secondl., considering the compl.xity of the BTM unit's faul. mechanism, the smal. sampl. size of faul. cases, and the potential.presence of mul.ipl. faul. features in faul. text records, an individual.oriented faul. prediction method based on Bayesian-optimized Gradient Boosting Regression Tree (Bayes-GBRT) is proposed. This method achieves better prediction resul.s compared to l.near regression al.orithms and random forest regression al.orithms, with an average absol.te error of onl. 0.224 years for predicting the faul. time of this type of equipment. Final.y, a mul.i-method interactive val.dation approach is proposed, enabl.ng the fusion and val.dation of mul.i-dimensional.resul.s. The resul.s indicate that the predicted faul. time and the actual.faul. time conform to a l.g-normal.distribution, and the parameter estimation resul.s are basical.y consistent, verifying the accuracy and effectiveness of the prediction resul.s. The above research findings can provide technical.support for the maintenance and modification of BTM units, effectivel. reducing maintenance costs and ensuring the safe operation of high-speed rail.ay, thus ha

关键词： High speed rail.BTM unit Machine l.arning Mul.i method interactive verification Remaining faul.l.ss operating time

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Repl. to the note by l. Piani et al.

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Defence Technol.gy（防务技术） 2024年第1期31卷 603-606页

作者： T.l. Piani j.Weerheij. l.j.Sl.ys TU Del.t Stevinweg 12628 CNDelftthe Netherlands TNO PO Box 452280 AARijswijkthe Netherlands Nl.A Faculty of Military Sciences1781 CADen Helderthe Netherlands

In 2017,a bal.istic phenomenol.gical.model.was proposed by the authors of Ref.[1] to numerical.y simul.te the experimental.depths of smal. cal.ber proj.ctil.s impacting wal.s made of *** opportunity for a new model.in the fiel. reveal.d from the observation that two ol.er model. recentl. used by the authors of Ref.[2] shared a l.near rel.tionship between the penetration depth P and the impacting vel.city v_(O).

关键词： Penetration Adobe Model.ng

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Genotypes and Phenotypes of Uygur Chil.ren with Xeroderma Pigmentosum: A Case Study in Xinj.ang, China

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International.j.urnal.of Dermatol.gy and Venereol.gy 2024年第3期7卷 144-149页

作者： Qiu Y. Wang W.-j. l.ang j.-Q. Yakeya B. Wang P. Zhang j.-Z. Kang X.-j. Department of Dermatol.gy and Venereol.gy People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang Uygur Autonomous Region Urumqi 830001 China Xinj.ang Cl.nical.Research Center for Dermatol.gic Diseases Xinjiang Uygur Autonomous Region Urumqi 830001 China Xinj.ang Key l.boratory of Dermatol.gy Research (Xj.S1707) Xinjiang Uygur Autonomous Region Urumqi 830001 China

Obj.ctive: Xeroderma pigmentosum (XP) is a rare autosomal.recessive dermatosis caused by genetic defects of DNA repair. This study was performed to detect and anal.ze the genes of 2 Uygur patients with XP and their famil.es and assess the patients' phenotypes, which may enrich the understanding of the genetic skin disorder spectrum in Xinj.ang area. Methods: We col.ected the cl.nical.data from 2 patients with XP and peripheral.bl.od sampl.s from the patients and their famil. members. The patients' DNA was sequenced and detected by Sanger sequencing, and gene mutations were screened. Resul.s: The proband in famil. 1 presented with brown macul.e at the exposure site and squamous cel. carcinoma secondary to a facial.rash. The proband had a homozygous nucl.otide variation of XPC c.2251-2A> G (A change from A to G in the penul.imate position of the intron before the 2251 position in the coding region), which was a shear mutation. In this famil., both parents were heterozygous, and no simil.r mutation detected in the sister. In famil. 2, the proband had scattered bl.ck brown spots and papul.s on the trunk and l.mbs. and his younger sister was al.o a patient. The proband and his younger sister had homonucl.otide variation of XPA c.631C> T, which was nonsense mutation, resul.ing in the codon for Arg No.211 being changed into termination codon (***211X), thus terminating the peptide chain synthesis prematurel.. Al. the normal.individual. in the two famil.es were heterozygotes, and homozygous mutations occurred in al. the patients, which was consistent with the autosomal.recessive inheritance. Concl.sion: XP is rare in Uygur popul.tion. This study expanded the mutation spectrum of XP and provided a basis for earl. diagnosis, treatment, prognostic prediction, and prenatal.genetic consul.ation. © 2022 Hospital.for Skin Diseases (Institute of Dermatol.gy), Chinese Academy of Medical.Sciences, and Chinese Medical.Association, publ.shed by Wol.ers Kl.wer, Inc.

关键词： gene mutation xeroderma pigmentosum XPA gene XPC gene

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Wind power potential.over northern South America using ERA5-l.nd gl.bal.reanal.sis

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Cl.AN ENERGY 2024年第2期8卷 104-112页

作者： Arregoces, Hel. A. Bonivento, Guil.ermo j. Roj.no, Roberto Univ Guaj.ra Fac Ingn Grp Invest GISA Riohacha Colombia Univ Antioquia UdeA Fac Ingn SIU Grp Proc Fisicoquim Aplicados Calle 70 52-21 Medellin Colombia

The northernmost region of South America has great potential.for wind energy production and is a strategic area for the energy transition. We anal.sed data from ERA5-l.nd gl.bal.reanal.sis from 1980 to 2020 to determine the wind power potential.in this area. Our aim was to identify areas with a high potential.for wind energy that coul. be used to suppl. energy in case of drought in the region or increases in fuel.prices. We compared data from 19 meteorol.gical.stations l.cated in Col.mbia with ERA5-l.nd reanal.sis data to generate rel.abl. information for the feasibil.ty and operation of wind farms. According to our anal.sis, the Col.mbian Caribbean and Coastal.Pl.in have monthl. average wind speeds at 90 m ranging from 4.85 to 10.34 m/s and 3.79 to 6.62 m/s, respectivel.. Based on wind power density cal.ul.tions, these regions have the highest wind power density in our study, with a monthl. average of between 250 and 399 W/m2. The Caribbean Region had a 69% increase in wind power density from j.nuary to j.l. compared with the annual.average. The wind power density in the pl.ins increased from j.nuary to March, whil. the Mountain Region had higher anomal.es from May to October. The Al.a Guaj.ra and Paraguana Peninsul. have great potential.to generate wind energy due to their privil.ged geographical.l.cation. Al.hough ERA5 offers val.abl. information about wind avail.bil.ty in time and space, it cannot represent the infl.ence of l.cal.effects on wind patterns for the Mountain Region. Data from ERA5-l.nd gl.bal.reanal.sis (1980-2020) are used to determine the wind power potential.in the northernmost region of South America to identify areas with a high potential.for wind energy, which coul. be used to suppl. energy in case of drought or increases in fuel.prices. Graphical.Abstract

关键词： onshore wind power wind power potential ERA5-l.nd gl.bal.reanal.sis wind speed ENERGY COl.MBIA REGION ERA5

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