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De novo assembly of a new Olea europaea genome accession using nano pore seque ncing

Horticulture Research 2021年第1期8卷 1179-1190页

作者： Guodong rao Jianguo Zhang Xiaoxia Liu Chunfu Lin Huaigen Xin Li Xue Chenhe Wang State Key Laboratory of Tree Genetics and Breed ing Research Institute of ForestryChinese Academy of ForestryBeijing 100091China Collaborative Innovation Center of Susta in able Forestry in Southern China Nanjing Forestry UniversityNanjing 210037China Key Laboratory of Tree Breeding and Cultivation National Forestry and Grassland AdministrationResearch Institute of ForestryChinese Academy of ForestryBeijing 100091China MIAnnInG Yuansheng Agricultural Science and Technology Co. Ltd.Liangshan Yi Autonomous Prefecture Mianning CountySichuan 615600China Biomarker Technologies Corporation Beijing 101300China

Olive(Olea europaea L.)is internationally renowned for its high-end product,extra virgin olive *** incomplete genome of *** was previously obtained using shotgun sequencing in *** further explore the genetic and breeding utilization of olive,an updated draft genome of olive was obtained using Oxford nanopore third-generation sequencing and Hi-C *** different assembly strategies were used to assemble the fi nal genome of 1.30 Gb,with contig and scaffold n50 sizes of4.67 Mb and 42.60 Mb,*** greatly increased the quality of the olive *** assembled 1.1 Gb of sequences of the total olive genome to 23 pseudochromosomes by Hi-C,and 53,518 protein-coding genes were predicted in the current *** genomics analyses,including gene family expansion and contraction,whole-genome replication,phylogenetic analysis,and positive selection,were *** on the obtained high-quality olive genome,a total of nine gene families with 202 genes were identi fi ed in the oleuropein biosynthesis pathway,which is twice the number ofgenes identi fi ed from the previous *** new accession of the olive genome is of suf fi cient quality for genome-wide studies on gene function in olive and has provided a foundation for the molecular breeding of olive species.

关键词： breeding Olea assembly

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Synthesis and micellization behavior of stimuli-responsive polypeptide hybrid triblock copolymer

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Chinese Science Bulletin 2009年第8期54卷 1912-1917页

作者： rao JingYi ZHU ZhiYuan LIU ShiYong Hefei national Laboratory for Physical Sciences at the Microscale CAS Key Laboratory of Soft Matter Chemistry Department ofPolymer Science and Engineering University of Science and Technology of China Hefei 230026 China

Polypeptide hybrid triblock copolymer, poly(L-glutamic acid)-b-poly(propylene oxide)-b-poly (L-glu-tamic acid) (PLGA-b-PPO-b-PLGA), was synthesized by the ring-opening polymerization of benzyl-L-glutamic n-carboxyanhydride (BLG-nCA) using poly(propylene glycol) bis(2-aminopropyl ether) as initiator, followed by the subsequent deprotection step. The obtained double hydrophilic triblock co-polymer exhibits "schizophrenic" micellization behavior in aqueous solution upon dually playing with solution pH and temperature. The multi-responsive micellization behavior of this polypeptide hybrid triblock copolymer has been thoroughly investigated by 1H nMR, laser light scattering (LLS), tempera-ture-dependent optical transmittance, and circular dichroism spectroscopy (CD).

关键词：三嵌段共聚物胶束化行为混合多肽合成温度依赖性反应雷电定位系统

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Hot forging of Mg-4Al-2Ba-2Ca(ABa X422) alloy and validation of processing map

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Transactions of nonferrous Metals Society of China 2018年第8期28卷 1495-1503页

作者： K.SURESH K.P.rao Y.V.R.K.PRASAD n.HORT H.DIERInGA Department of mechanical and Biomedical Engineering City Uiliversity of Hong Kong Tat Chee Avenue Kowloon Hoilg Koilg China *** no. 2/B vinayaka Nagar Hebbal Bengalum 560024 India Helmholtz-zentrum Geesthacht institute of Materials ResearchMagnesium innovation Centre Max-Planck-StraBe 1 Geesthacht 21502 Germany

A cup-shaped component of Mg-4 Al-2 Ba-2 Ca（ABa X422） alloy was forged in the temperature range of 300-500 °C and at speeds in the range of 0.01-10 mm/s with a view to validate the processing map and study the microstructural development. The process was simulated through finite-element method to estimate the local and average strain rate ranges in the forging envelope. The processing map exhibited two domains in the following ranges：（1） 300-390 °C and 0.0003-0.001 s^-1, and（2） 400-500 °C and 0.0003-0.3 s^-1 and both represented dynamic recrystallization（DRX）. The map revealed a wide flow instability regime at higher strain rates and temperatures lower than 400 °C, in which flow localization occurred. Forgings produced under conditions of the above two domains were sound and symmetrical, and had finer grain sizes when being forged in the first domain. However, when being forged in the flow instability regimes, the alloy fractured before the final shape was reached. The experimental load-stroke curves for the conditions within the domains correlated well with the simulated ones, whereas the curves obtained in the instability regime were uneven.

关键词： magnesium alloy, processing map hot forging dynamic recrystallization

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Microstructure and mechanical properties of as-cast Mg-Sn-Ca alloys and effect of alloying elements

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Transactions of nonferrous Metals Society of China 2013年第12期23卷 3604-3610页

作者： K.SURESH K.P.rao Y.V.R.K.PRASAD n.HORT K.U.KAInER Department of Mechanical and Biomedical Engineering City University of Hong Kong Consultant *** No. 2/B Vinayaka Nagar Helmholtz-Zentrum Geesthacht Institute of Materials Research Magnesium Innovation Centre

The effect of Sn, Ca, Al, Si and Zn addition on the compressive strength of cast Mg-Sn-Ca （TX） alloys was studied in the temperature range of 25-250 °C and correlated with the microstructure. The Sn to Ca mass ratio up to 2.5 contributes to the formation of Mg2Ca phase at the grain boundaries and CaMgSn in the matrix, while a ratio of 3 gives only CaMgSn phase mostly in the matrix. While the compressive strength decreases with the increase in temperature, for Sn/Ca up to 2.5, a plateau occurs in 100-175 °C, which is attributed to the strengthening by Mg2Ca. However, for ratio of 3, the strength is lower and decreases more gradually. Mg-3Sn-2Ca （TX32） has the highest strength and the addition of 0.4%Al increases its strength but simultaneous addition of Si lowers the strength. Likewise, the addition of Zn improves its strength but simultaneous addition of Al slightly decreases the strength. The results are correlated with the types of intermetallic phases that form in various alloys.

关键词： Sn/Ca ratio Mg-Sn-Ca alloys alloying additions microstructure compressive strength

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COMADEM: A Proactive Integrated Multidiscipline for the 21st Century

COMADEM: A Proactive Integrated Multidiscipline for the 21st...

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第十二届全国设备监测与诊断学术会议

作者： B．K．n.rao D．Tech COMADEM International 307 Tiverton RoadSelly OakBirmingham B29 6DAUK Prof．Dr． COMADEM International 307 Tiverton RoadSelly OakBirmingham B29 6DAUK PhD

＜正＞Rapid changes in the fields of science, engineering, technology and management on a global scale are taking place. As a consequence of this great challenges are emerging to be urgently addressed by educators, professional/R ＆ D organisations, government bodies and industrial communities worldwide . COndition Monitoring And Diagnostic Engineering Management （COMADEM） is a fast-emerging proactive multidiscipline, which addresses important current global issues in engineering education and training at all levels. Since its inception in 1988, the philosophical and the practical aspects of COMADEM have been widely propagated to the academic, professional and industrial communities worldwide. This has resulted in the establishment of various undergraduate/postgraduate/doctoral/funded research/development programmes *** such dynamic programmes, difficult solutions to a number of problems have been proactively and profitably resolved by implementing novel, innovative and cost-effective hardware/*** technical literature in terms of patents, doctoral thesis, undergraduate/postgraduate research reports, textbooks/handbooks/conference proceedings and journal papers （ both refereed and commercial） are profusely growing at an exponential rate. This trend is growing unabated. A number of annual/biennial international conferences and exhibitions are now organised in different parts of the world focusing on various aspects of this interdiscipline. Successful and continued efforts are being made to reinforce/explore/exploit the positive virtues of this multidiscipline for maximum benefits through international academic/industrial collaborative/partnership *** paper will present the accumulated research findings, valuable experiences gained and to reflect some thoughts into the future of engineering education and training. A change of culture in engineering education and training worldwide is stressed to produce a high quality, well-rounded and valua

关键词： COMDEM Condition Monitoring Diagnostic Engineering Proactive Management Forensic Engineering Asset Management Engineering Education: Engineering Training Maintenance Engineering Maintenance Management Technology Management Engineering Management.

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An Integrated Geoscientific Approach to Define Zones of Gold Mineralization <br/>—A Case Study in north Sudan

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Open Journal of Geology 2014年第5期4卷 190-197页

作者： Bijay K. Mishra n. Sulekha rao Sudeep Rautray Subhranshu Pattanayak RMSI Private Limited Auriga Software Units Layout Hyderabad India

The study area is located in Bayuda desert lying about 400 km from Khartoum in the state of River nile, north Sudan. The present study aimed to identify gold mineralized zones through an integrated geoscientific approach. The approach integrated the results of geospatial studies, geological mapping, field observations and geochemical data. Remote sensing studies using ASTER and Quickbird data helped to identify the zones of chloritic, sericitic and phyllic alteration signatures and gossan rich zones. Geological mapping and field observations confirmed the presence of gold mineralization along the quartz veins which shared the litho contacts of biotite muscovite schist, biotite muscovite gneiss and silicate gneiss. These gold bearing potential areas were found to be structurally controlled and most of the mineralizations were along the proximity of shear-zones, faults and folds. The gold assay values obtained from the chemical analysis of stream sediment and pitting-trenching samples also confirmed the results of remote sensing and geological mapping. Integration of all these data helped to identify drill holes’ locations in the study area for further exploration. This focused plan facilitated cost and time optimization in exploration activities.

关键词： Remote Sensing Geological Geochemical Gold Hydrothermal Alterations Gossans Quartz Veins

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Geochemical evidences of trace metal anomalies for finding hydrocarbon microseepage in the petroliferous regions of the Tatipaka and Pasarlapudi areas of Krishna Godavari Basin,India

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Petroleum Science 2013年第1期10卷 19-29页

作者： Rasheed M A Lakshmi M rao P L S Kalpana M S Dayal A M Patil D J Microbiology Laboratory Petroleum Geochemistry Group National Geophysical Research Institute (Council of Scientific and Industrial Research)

The long-term seepage of hydrocarbons, either as macroseepage or microseepage, can set up near-surface oxidation reduction zones that favor the development of a diverse array of chemical and mineralogical changes. The bacterial oxidation of light hydrocarbons can directly or indirectly bring about significant changes in the values of pH and Eh of the surrounding environment, thereby also changing the stability fields of the different mineral species present in that environment. The paper reports the role of hydrocarbon microseepage in surface alterations of trace metal concentrations. In this study trace metal alterations were mapped that appear to be associated with hydrocarbon microseepages in the oil/ gas fields. A total of 50 soil samples were collected near oil and gas fields of the Tatipaka and Pasarlapudi areas of the Krishna Godavari Basin, Andhra Pradesh. The soil samples were collected from a depth of 2-2.5 m. The paper reports the chemical alterations associated with trace metals in soils that are related to hydrocarbon microseepages above some of the major oil and gas fields of this petroliferous region. Trace metals, such as scandium （Sc）, vanadium （V）, chromium （Cr）, cobalt （Co）, nickel （ni）, copper （Cu）, zinc （Zn）, barium （Ba） and strontium （Sr）, in soil samples were analyzed using inductively coupled plasma- mass spectrometry （ICP-MS）. The concentrations of Sc （8 to 40 mg/kg）, V （197 to 489 mg/kg）, Cr （106 to 287 mg/kg）, Co （31 to 52 mg/kg）, ni （65 to 110 mg/kg）, Cu （88 to 131 mg/kg）, Zn （88 to 471 mg/kg）, Ba （263 to 3,091 mg/kg） and Sr （119 to 218 mg/kg） were obtained. It was observed that the concentrations of trace elements were tremendously increased when they were compared with their normal concentrations in soils. The analysis of adsorbed soil gas showed the presence of high concentrations of YC2＋（C2H6, C3H8 and n-C4H10） ranging from 7 to 222 μg/kg respectively. Integrated studies of trace elements over adsorbed light gaseous hydrocarbons （2C2

关键词： Hydrocarbons microseepage trace metal alterations adsorbed soil gas Krishna Godavari Basin

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An Efficient and Green Approach to Synthesizing Enamines by Intermolecular Hydroamination of Activated Alkynes

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Chemical Research in Chinese Universities 2015年第2期31卷 212-217页

作者： ZEnG Ruijie SHEnG Hongting rao Bo FEnG Yan WAnG Hao SUn Yehua CHEn Man ZHU Manzhou School of Chemistry and Chemical Engineering Anhui University Hefei 230601 P. R. China

An efficient, atom-economic and green approach to synthesizing enamines was developed by intermolecular hydroamination of activated alkynes with high yields under catalyst- and solvent-free conditions. β-Dimethylamino-acrylate derivatives were also obtained with high yields. In the synthetic process of the derivatives, n,n-dimethylformamide（DMF） pretreated with metal na, was used as reactant instead of dimethylamine gas. The proposed synthetic method can be used for the synthesis of （E）-ethyl-3-（dimethylamino）acrylate（3cl）, and provide a new possible way to the synthesis of Quinolones.

关键词： Enamine Hydroamination Activated alkyne Catalyst-free Solvent-free n,n-Dimethylformamide

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Characterization of Bacillus subtilis Ab03 for efficient ammonia nitrogen removal

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Systems Microbiology and Biomanufacturing 2022年第3期2卷 580-588页

作者： Qiang Wang Weilai Fu Ruiqi Lu Chunli Pan Ganfeng Yi Xian Zhang Zhiming rao Jiangnan University WuxiJiangsu ProvinceChina Fujian DBn Aquatic Science and Technology CO.LTD Zhao-AnChina

An efficient ammonia nitrogen degrading bacterial strain was isolated from a fish and shrimp pond and identified as Bacillus subtilis(Ab03).Firstly,the strain was continuously domesticated in ammonium solution to improve its nitrogen removal *** performance of the strain in terms of nitrogen removal efficiency under different culture conditions was then ***,the nitrogen removal process and related strain mechanisms were analyzed by nitrogen *** results showed the strain Ab03 could remove 91.67% of nH_(4)^(+)-n at 300 mg/L under the conditions of glucose as the single carbon source,C/n of 15,pH of 7.5,the temperature of 35℃ and dissolved oxygen of 7-8 mg/*** was also found that under conditions where ammonia nitrogen was the only nitrogen source,strain Ab03 could also undergo aerobic denitrification for simultaneous conversion,with a final gas conversion rate of 74.81%.

关键词： Bacillus subtilis Ammonia nitrogen nitrogen balance Simultaneous conversion

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Soil Enzyme Activities and Organic Matter Composition Affected by 26 Years of Continuous Cropping

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Pedosphere 2016年第5期26卷 618-625页

作者： Fugen DOU Alan L.WRIGHT rao S.MYLAVARAPU JIAnG Xianjun John E.MATOCHA Texas A&M AgriLife Research Center at Beaumont BeaumontTX 77713(USA) University of Florida Indian River Research&Education CenterFt.PierceFL 34945(USA) University of Florida Soil&Water Science Dept.GainesvilleFL 32611(USA) Southwest University College of Resources and EnvironmentChongqing(China) Texas A&M University Agricultural Research and Extension CenterCorpus ChristiTX 78406(USA)

The study was to determine the long-term effects of subtropical monoculture and rotational cropping systems and fertilization on soil enzyme activities and soil C, n, and P levels. Cropping systems included continuous sorghum(Sorghum bicolor L.), cotton(Gossypium hirsutum L.), corn(Zea mays L.), and cotton/sorghum rotations after 26 years of treatment imposition. Soil under continuous sorghum and continuous corn had 15% and 11%, respectively, greater C concentrations than soil under continuous *** C was 10% higher at 0–7.5 cm than at 7.5–15 cm. Total n followed similar trends with soil depth as organic C. Continuous sorghum had 19% higher total n than other crop species and rotations. With fertilization, continuous cotton had the highest total P at 0–7.5 cm and sorghum had the highest at 7.5–15 cm. Soil total P was 14% higher at 0–7.5 than at 7.5–15 cm, and fertilization increased 15% total P compared to unfertilized soil. Arylsulfatase, alkaline phosphatase, and β-d-glucosidase activity were the highest for sorghum and the lowest for cotton. Rotation increased enzyme activities compared to continuous cotton but not for continuous sorghum. Of all crop species and rotations, continuous cotton generally showed the lowest levels of organic matter and enzyme activities after 26 years. Fertilization significantly increased the yields for all cropping systems, but rotation had no significant effect on either sorghum or cotton lint yield compared to each crop grown in monoculture. Long-term cropping did not increase soil organic matter levels beyond short-term gains, indicating the difficulty in promoting C sequestration in subtropical soils.

关键词： C sequestration fertilization monoculture rotation subtropical soil

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