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Bovine sperm selection procedure prior to cryopreservation for improvement of post-thawed semen quality and fertility

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Journal of Animal Science and Biotechnology 2020年第1期11卷 44-57页

作者： MariaPortia B.Nagata Junki Egashira Naoto Katafuchi Kenji endo Kazuko Ogata Kenichi Yamanaka Tadayuki Yamanouchi Hideo Matsuda Yutaka Hashiyada Kenichi Yamashita Advanced Manufacturing Research Institute National Institute of Advanced Industrial Science and Technology(AIST)807-1 Shuku-machiTosuSaga 841-0052Japan Saga Prefectural Livestock Experiment Station 23242-2 Yamauchi-machiMiyanoTakeoSaga 849-2305Japan Morinaga Dairy Service Co.Ltd. 1-159 ToyoharaotsuNasu-gun Nasu-machiTochigi 329-3224Japan National Livestock Breeding Center(NLBC) 1 OdakuraharaOdakuraNishigo-muraNishishirakawa-gunFukushima 961-8511Japan Institute of Livestock and Grassland Science National Agriculture and Food Research Organization(NARO)Ikenodai 2TsukubaIbaraki 305-0901Japan Faculty of Agriculture Saga University1 Honjo-machiSaga 840-8502Japan.7Ishikawa Prefectural University1-308 SuematsuNonoichi-shiIshikawa 921-8836Japan.

Background:The application of cryopreservation and artificial insemination technology have contributed to the advancement of animal ***,a substantial proportion of spermatozoa undergoes alterations and loses their fertility during cryopreservation,rendering the frozen-thawed semen impractical for routine *** is known to reduce sperm lifespan and *** in cryosurvival of spermatozoa from different sires and even with the individual sire is common in artificial insemination(AI)*** goal is to improve post-thawed semen quality by optimization of cryopreservation technique through sperm selection prior to cryopreservation ***:Our strategy of sperm selection based on rheotaxis and thermotaxis(SSRT)on macrosale in a rotating fluid flow demonstrated the ability to maintain the original pre-freezing structural integrity,viability and biological function related to fertilization *** strategy has a positive effect on the cryosurvival and fertilizing abilities of spermatozoa as supported by the improvement on pregnancy rate of Japanese Black heifers and Holstein repeat *** technique protected further sublethal damage to bovine spermatozoa(higher%cryosurvival than the control)and resulted in the improvement of DNA *** selected spermatozoa demonstrated slower and controlled capacitation than unprocessed control which is thought to be related to sperm longevity and consequently to appropriate timing during in vivo ***:These results provide solid evidence that improvement of post-thawed semen quality by SSRT method is beneficial in terms of cryosurvival,longevity of post-thawed sperm,and optimization of in vivo fertilization,embryo development and calving as supported by the favorable results of field fertility study.

关键词： Cryopreservation Fertility Livestock Rheotaxis Spermatozoa Thermotaxis

导电薄层下三维岩脉的CSAMT响应分析(英文)

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地球物理学报 2010年第3期53卷 677-684页

作者：王若 Masashi endo 底青云王妙月中国科学院地质与地球物理研究所中国科学院工程地质力学重点实验室北京100029 Consortium for Electromagnetic Modeling and Inversion(CEMI) University of Utah

为了研究探测3D导电岩脉状目标体的可控源音频电磁法(CSAMT)的最佳观测装置,我们用压缩积分方程法数值模拟了赋存于导电薄层下的3D导电岩脉的电磁场响应.计算结果表明,最有效的源的位置是把发射源沿岩脉的走向放置.相对于其他几种装置,... 详细信息

为了研究探测3D导电岩脉状目标体的可控源音频电磁法(CSAMT)的最佳观测装置,我们用压缩积分方程法数值模拟了赋存于导电薄层下的3D导电岩脉的电磁场响应.计算结果表明,最有效的源的位置是把发射源沿岩脉的走向放置.相对于其他几种装置,这种观测装置得到的异常场与主场的比值最大.利用这种最佳的观测装置,通过将异常场E_(ya)和H_(xa)与噪音作对比,研究了当岩脉的埋深和高度发生变化时岩脉的可探测性问题.当岩脉的顶部埋深增加时,导电岩脉的可探测性降低.岩脉高度的变化对异常场幅值的影响要小于岩脉顶部埋深所产生的影响.所以通常情况下,即便应用了最有效的CSAMT装置,也很难探测到岩脉的高度,应用钻孔中的探测可能会解决这个问题.

关键词： 3D岩脉模型压缩积分方程法最佳装置影响因素

Important roles of graphene edges in carbon-based energy storage devices

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Journal of Energy Chemistry 2013年第2期22卷 183-194页

作者： Yoong Ahm Kim Takuya Hayashi Jin Hee Kim Morinobu endo Faculty of Engineering Shinshu University 4-17-1 Wakasato Nagano 380-8553 Japan Research Center for Exotic Nanocarbons (JST) Shinshu University 4-17-1 Wakasato Nagano 380-8553 Japan

Nanostructured carbon materials, including carbon nanotubes, graphene and nanoporous carbon, show promise for expanding renewable energy. In particular, the configuration and electronic properties of graphene edges in relation with their electrochemical activity have become a major issue in carbon-based energy storage devices. Here, we review recent results concerning the important roles of graphene edges as the gateway for lithium ion intercalation in the anode of lithium-ion batteries, as promoters of high capacitance in carbon-based supercapacitors, and as anchoring sites for Pt nanoparticles in fuel cells. We envisage that the controlled synthesis of a specific, clean, and stable edge configuration could be achieved to maximize the electrochemical performance of nanostructured carbon-based energy storage devices.

关键词： carbon nanotube graphene edge lithium ion battery supercapacitor fuel cell

A case-control study on relationship between arsenic in drinking water and skin lesions in endemic arsenism area of Shanxi province,China

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A case-control study on relationship between arsenic in drin...

Barrier component and driving force component of transepidermal water loss and their application to skin irritant responses

中国毒理学会第五次全国学术大会

作者： endo Yoko endo Ginji Yamanaka Kenzo Research Center for Occupational Poisoning Tokyo Rosai HospitalTokyo 143-0013 Japan Osaka City University Medical School 545-8585 Osaka Nihon University College of Pharmacy 275-8555 Funabasgi Japan

＜正＞Chronic arsenic exposure in human is associated with symptoms of skin lesions,including of hyperpigmentation （melanosis）,hypopigmentation,keratosis,hyperkeratosis skin ulceration,and skin cancers（Bowen’s disease）.The evidence of epidemiologic data was not *** the current research, the relationship between arsenic in drinking water and skin lesions in endemic arsenism area of Shanxi province was carried out in China.203 cases and 120 controls were selected in 19 endemic arsenism villages in Shanxi *** content of arsenic in drinking water which was collected indoors was half-quantitatively screened by kit made by Chinese Center for Disease Control and Prevention,then quantitatively determined by HPLC-ICP-MS *** of endemic arsenism were diagnosed by the standard of diagnosis for endemic *** analysis was carried out using SAS software（version 9.13,SAS institute,Inc.,Cary,NC,USA）.There are 64.2%samples above the arsenic level（50μg/L）of national drinking water and the median value of arsenic in drinking water is 96.3μg/L in 201 water *** OR（95%CI）value between arsenic in drinking water and hyperkeratosis,hyperpigmentation,depigmentation and skin lesions is 1.908（1.442-2.524）,2.058（1. 460-2.900）,3.086（2.316-4.112）,3.142（2.434-4.056）, *** values of OR are statistically *** the increasing of arsenic in drinking water,the prevalence of skin lesions（p＜0.05） and the degree of skin lesions increased （p＜0.05）.There is positive dose-response relationship between arsenic in drinking water and skin *** is a medial arsenic pollution *** current epidemiological research showed the high concentration of iron in drinking water enhanced the arsenic-related skin lesions,especially when the concentration of arsenic in drinking water is lower than 50μg/L.

关键词： Arsenism Skin lesions Case-control study

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Barrier component and driving force component of transepider...

Flume experiments on responses of topography at a river mouth to change in external conditions

2006中国中西医结合皮肤性病学术会议

作者： Koji endo Analytical Research Center KAO Corporation2606AkabaneIchikai-machiHagaTochigi321-3497Japan

INTRODUCTION Spontaneous water exchange between skin and ambient atmosphere is known as transepidermal water loss (TEWL). The principal barrier to water loss resides in the stratum corneum (SC) , the outermost thin layer (10 - 20 μm thickness) of the skin, which is composed of keratinized cells embedded in a unique lipid matrix . TEWL rate has been measured in vivo using an evaporimeter, a closed chamber method and a ventilated

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地球环境学报 2011年第1期 311-312页

作者： endo N Suzuki T Department of Earth Sciences Graduate School of Natural Science and TechnologyKanazawa University

Flume experiments in which the silty deltas were developed under unsteady flows were *** examined several increasing and decrease rates of *** showed that sedimentary processes of a delta were aggradation at high increase rates of discharge,backstepping at middle rates,and progradation at low *** the phase of decreasing flow,there was hysteresis in the development *** if the decrease rate was the same,the process of topographic change was different according to the previous state of the delta *** we found that the development processes related with the sediment balance(erosion-sedimentation) on the topset bed that was quasi-equilibrium state at low increase rates of flow discharge,but was non-equilibrium state at high increase rates of discharge.

关键词： Flume experiment river delta

Therapeutic efficacy of the Qing Dai in patients with intractable ulcerative colitis

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World Journal of Gastroenterology 2013年第17期19卷 2718-2722页

作者： Hideo Suzuki Tsuyoshi Kaneko Yuji Mizokami Toshiaki Narasaka Shinji endo Hirofumi Matsui Akinori Yanaka Aki Hirayama Ichinosuke Hyodo Department of Gastroenterology Institute of Clinical Medicine University of Tsukuba Graduate School Center of Integrative Medicine Tsukuba University of Technology

Ulcerative colitis (UC) is a chronic inflammatory bowel disease that may become intractable when treated with conventional medications such as aminosalicylates, corticosteroids, and azathioprine. The herbal medicine Qing Dai has traditionally been used in Chinese medicine to treat UC patients, but there is a lack of published data on the efficacy of Qing Dai in UC treatment. We report several cases of patients with intractable UC who take Qing Dai in a retrospective observational study. Furthermore, we explore the mechanisms of action of Qing Dai. Nine patients with active UC who received conventional medications but wished to receive Qing Dai as an alternative medication were included in our analysis. The UC severity level was determined based on the clinical activity index (CAI). Additionally, 5 of the 9 patients were endoscopically evaluated according to the Matts grading system. Each patient received 2 g/d of Qing Dai orally and continued taking other medications for UC as prescribed. Electron spin resonance was applied to explore the mechanisms of action of Qing Dai. After 4 mo of treatment with Qing Dai, the CAI score decreased from 8.3 ± 2.4 to 2.4 ± 3.4 (mean ± SD; P endoscopic Matts grade decreased from 3.4 ± 0.5 to 2.2 ± 0.8 (P = 0.02). Six of 7 patients who were on prednisolone upon enrollment in the study were able to discontinue this corticosteroid. Electron spin resonance revealed that Qing Dai possesses strong hydroxyl radical scavenging activity. Qing Dai showed significant clinical and endoscopic efficacy in patients who failed to respond to conventional medications. Scavenging of hydroxyl radicals appears to be a potential mechanism through which Qing Dai acts, but the significance of the scavenging ability of Qing Dai with respect to the anti-inflammatory effect in UC patients warrants further investigation.

关键词： Qing Dai Herbal medicine Ulcerative colitis Hydroxyl radical Electron spin resonance

氟-18-左旋氧氟沙星的标记与动物实验研究

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核技术 2006年第7期29卷 537-540页

作者：袁志斌 Katsumi TOMIYOSHI Tetsuya HIGUCHI Noboru ORIUCHI Keigo endo 上海交通大学附属第六人民医院核医学科上海200233 日本群马大学医学部核医学

炎症显像是核素影像诊断的重要研究领域,但其中正电子核素标记的药物报道不多。为探索合成一种可特异性在炎症部位浓集、且可鉴别是否为细菌性感染的正电子核素标记的显像剂,我们研究制备了F标记18的Levofloxacin(LVFX)用于炎症显像。... 详细信息

炎症显像是核素影像诊断的重要研究领域,但其中正电子核素标记的药物报道不多。为探索合成一种可特异性在炎症部位浓集、且可鉴别是否为细菌性感染的正电子核素标记的显像剂,我们研究制备了F标记18的Levofloxacin(LVFX)用于炎症显像。标记方法采用经典的Hamacher法(氟离子亲核取代)标记LVFX,放射化学纯大于98%,放射性得率约40%,热原检测为阴性。结果表明标记方法简单迅速,标记物稳定。研究了F-LVFX在正常炎症动物模型内的生物分布以及炎症动物模型的显像。生物分布和显像结果均表明1818F-LVFX在炎症组织内有较高的摄取,炎症组织与正常组织间有显著性差异(P<0.05),该标记物主要通过肾脏和肝脏排泄。研究表明F-LVFX是一种潜在的炎症显像剂。

关键词：左旋氧氟沙星炎症动物核素显像

En bloc pancreaticoduodenectomy and right hemicolectomy for locally advanced right-sided colon cancer

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World Journal of Gastrointestinal Oncology 2017年第9期9卷 372-378页

作者： Yuji Kaneda Hiroshi Noda Yuhei endo Nao Kakizawa Kosuke Ichida Fumiaki Watanabe Takaharu Kato Yasuyuki Miyakura Koichi Suzuki Toshiki Rikiyama Department of Surgery Saitama Medical CenterJichi Medical UniversitySaitama 330-8503Japan

AIM To assess the usefulness of en bloc right hemicolectomy with pancreaticoduodenectomy(RHCPD) for locally advanced right-sided colon cancer(LARCC).METHODS We retrospectively reviewed the database of Saitama Medical Center, Jichi Medical University, between January 2009 and December 2016. During this time, 299 patients underwent radical right hemicolectomy for right-sided colon cancer. Among them, 5 underwent RHCPD for LARCC with tumor infiltration to adjacent organs. Preoperative computed tomography(CT) was routinely performed to evaluate local tumor infiltration into adjacent organs. During the operation, we evaluated the resectability and the amount of infiltration into the adjacent organs without dissecting the adherent organs from the cancer. When we confirmed that radical resection was feasible and could lead to R0 resection, we performed RHCPD. The clinical data were carefully reviewed, and the demographic variables, intraoperative data, and postoperative parameters were *** The median age of the 5 patients who underwent RHCPD for LARCC was 70 years. The tumors were located in the ascending colon(three patients) and transverse colon(two patients). Preoperative CT revealed infiltration of the tumor into the duodenum in all patients, the pancreas in four patients, the superior mesenteric vein(SMV) in two patients, and tumor thrombosis in the SMV in one patient. We performed RHCPD plus SMV resection in three patients. Major postoperative complications occurred in 3 patients(60%) as pancreatic fistula(grade B and grade C, according to International Study Group on Pancreatic Fistula Definition) and delayed gastric empty. None of the patients died during their hospital stay. A histological examination confirmed malignant infiltration into the duodenum and/or pancreas in 4 patients(80%), and no patients showed any malignant infiltration into the SMV. Two patients were histologically confirmed to have tumor thrombosis in the SMV. All of the tumors had c

关键词： Locally advanced right-sided colon cancer Right hemicolectomy Malignant infiltration Inflammatory adhesion Pancreaticoduodenectomy