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Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

World Journal of Stem Cells 2010年第6期2卷 127-140页

作者： Solomon Mamo Julianna Kobolak istvan Borbíró Tamás Bíró istvan bock Andras Dinnyes Genetic Reprogramming Group Agricultural Biotechnology CenterSzent-Gyorgyi Albert ut.4H-2100 GdllHungary Solomon MamoLyons Research FarmUniversity College DublinNewcastleCo DublinIreland Department of Physiology Research Center for Molecular MedicineMedical and Health Science CenterUniversity of DebrecenNagyerdei krt.98H-4032 DebrecenHungary Abiol Ltd. Nagyerdei krt.98H-4032 DebrecenHungary BioTalentum Ltd. Aulich l.26GdllHungary Andras DinnyesMolecular Animal Biotechnology LaboratorySzent Istvan UniversityPáter K.u.1H-2103 GdllHungary

AIM:To compare gene targeting efficiencies,expression profiles,and Ca2+ handling potentials in two widely used mouse embryonic stem cell ***:The two widely used mouse embryonic stem cell lines,R1 and HM-1,were cultured and maintained on Mitomycin C treated mouse embryonic fibroblast feeder cell layers,following standard culture *** were incubated with primary and secondary antibodies before fluorescence activated cell sorting analysis to compare known pluripotency ***,cells were harvested by trypsinization and transfected with a kinase-inactive murine Tyk2 targeting construct,following the BioRad and Amaxa transfection ***,the cells were cultured and neomycin-resistant cells were picked after 13 d of *** clones were screened twice by polymerase chain reaction(PCR) and finally confirmed by Southern blot analysis before *** gene expression profiles of more than 20 400 probes were also compared and significantly regulated genes were conf irmed by real time PCR *** handling potentials of these cell lines were also compared using various ***:We found signif icant differences in transfection eff iciencies of the two cell lines(91% ± 6.1% vs 75% ± 4.2%,P = 0.01).Differences in the targeting efficiencies were also significant whether the Amaxa or BioRad platforms were used for *** did not observe signif icant differences in the levels of many known pluripotency ***,our genomewide expression analysis using more than 20 400 spotted cDNA arrays identified 55 differentially regulated transcripts(P < 0.05) implicated in various important biological processes,including binding molecular functions(particularly Ca2+ binding roles).Subsequently,we measured Ca2+ signals in these cell lines in response to various calcium agonists,both in high and low Ca2+ solutions,and found signif icant differences(P < 0.05) in the regulation of Ca2+ homeostasis betwee

关键词： Embryonic stem cells Microarray Calcium Agonists Transfection Cell culture Pluripotency Gene targeting

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Selective Absorber Coatings and Technological Advancements in Performance Enhancement for Parabolic Trough Solar Collector

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Journal of Thermal Science 2022年第6期31卷 1990-2008页

作者： ASAAD YASSEEN Al-Rabeeah istvan Seres istvan Farkas Doctoral School of Mechanical Engineering Hungarian University of Agriculture and Life SciencesGodollo H-2100Hungary Institute of Mathematics and Basic Science Hungarian University of Agriculture and Life SciencesGodollo H-2100Hungary Institute of Technology Hungarian University of Agriculture and Life SciencesGodollH-2100Hungary

Parabolic trough solar collector systems are the most advanced concentrating solar power technology for large-scale power generation purposes. The current work reviews various selective coating materials and their characteristics for different designs in concentrating solar power. Solar selective absorbing coatings collect solar radiation and convert it to heat. To promote higher efficiency and lower energy costs at higher temperatures requires, this study aims to analyse the fundamental chemistry and thermal stability of some key coatings currently being used and even under investigation to find reasons for differences, information gaps and potential for improvement in results. In recent years, several novel and useful solar absorber coatings have been developed. However, qualification test methods such as corrosion resistance, thermal stability testing and prediction of service life, which have essential technical value for large-scale solar absorbers, are lacking. Coatings are used to enhance the performance of reflectors and absorbers in terms of quality, efficiency, maintenance and cost. Differentiated coatings are required as there are no uniformly perfect materials in various applications, working conditions and material variations. Much more knowledge of the physical and chemical properties and durability of the coatings is required, which will help prevent failures that could not be discovered previously.

关键词： Parabolic trough solar collector selective coatings absorber receivers optical properties thermal stability

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Isomorphisms of Cubic Cayley Graphs on Dihedral Groups and Sparse Circulant Matrices

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Acta Mathematica Sinica,English Series 2023年第4期39卷 618-632页

作者： istvan KOVACS UP IAM&FAMNIT University of PrimorskaMuzejski trg 26000 KoperSlovenia

We show that,up to isomorphism,there is a unique non-CI connected cubic Cayley graph on the dihedral group of order 2n for each even number n≥*** answers in the negative the question of Li whether all connected cubic Cayley graphs are CI-graphs(Discrete Math.,256,301-334(2002)).As an application,a formula is derived for the number of isomorphism classes of connected cubic Cayley graphs on dihedral groups,which generalises the earlier formula of Huang et *** with the particular case when n is a prime(Acta ***.,***.,33,996-1011(2017)).As another application,a short proof is also given for a result on sparse circulant matrices obtained by Wiedemann and Zieve(arXiv preprint,(2007)).

关键词： Cayley graph graph isomorphism dihedral group circulant matrix

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CI-Property for Decomposable Schur Rings over an Abelian Group

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Algebra Colloquium 2019年第1期26卷 147-160页

作者： istvan Kovacs Grigory Ryabov UP IAM University of Primorska Muzejski trg 2 6000 Koper Slovenia UP FAMNIT University of Primorska Glagoljaska 8 6000 Koper Slovenia Sobolev Institute of Mathematics Novosibirsk Russia Novosibirsk State University Novosibirsk Russia

A Schur ring over a finite group is said to be decomposable if it is the generalized wreath product of Schur rings over smaller groups. In this paper we establish a sufficient condition for a decomposable Schur ring over the direct product of elementary abelian groups to be a Ci-Schur ring. By using this condition we offer short proofs for some known results on the CI-property for decomposable Schur rings over an elementary abelian group of rank at most 5.

关键词： isomorphism CI-group Schur ring

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Contextual Meaning and Word Meaning

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外国语 2006年第5期29卷 18-32页

作者： istvan Kecskes State University of New York AlbanyU.S.A

The paper has three goals: 1) Explain the role of context and word in meaning construction and comprehension. 2) Present a model that can explain meaning generated by both monolingual and multilingual meaning construction systems. 3) Discuss how the model can be applied to explain major issues in pragmatics. Pragmatics is understood here in a narrow sense as defined by Sperber & Noveck: “...pragmatics is the study of how linguistic properties and contextual factors interact in the interpretation of utterances” (Sperber & Noveck 2004∶1). It is argued that world knowledge is available to interlocutors in two forms: as encapsulated in lexical items based on prior encounters and experience (conventionalized cognitive context), and as provided by the actual situational context framed by the situation in which the interaction takes place. Meaning formally expressed in the linguistic interactional context is created on-the-spot, and is the result of the interaction of the two sides of world knowledge and the actual situational context. The paper makes three claims∶ First, supremacy of context is not unconditional in language processing. Second, salient meaning rather than literal meaning of lexical units plays a central role in comprehension. Third, intercultural communication differs from intracultural communication in the conceptual content of conventionalized cognitive contexts rather than in communicative means.

关键词： meaning prompting conventionalized cognitive context actual situational context dynamic model of meaning

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Visual rating and the use of image analysis for assessing canopy density in a pecan provenance collection during leaf fall

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Journal of Forestry Research 2023年第6期34卷 1843-1854页

作者： Cristina Pisani Clive H.bock Jennifer Randall United States Department of Agriculture−Agricultural Research Service (USDA-ARS)Southeastern Fruit and Tree Nut Research Station(SEFTNRS)ByronGA 31008USA Department of Entomology Plant Pathology and Weed ScienceNew Mexico State UniversityLas CrucesNM 88003USA

A collection representing the native range of pecan was planted at the US DA-ARS Southeastern Fruit and Tree Nut Research Station,Byron,*** collection(867 trees)is a valuable genetic resource for characterizing important horticultural *** density during leaf fall is important as the seasonal canopy dynamics provides insights to environmental cues and breeding potential of *** ability of visual raters to estimate canopy density on a subset of the provenance collection(76 trees)as an indicator of leaf shed during autumn along with image analysis values was *** canopy density using the image analysis software was less compared to visual estimates(11.9%vs 18.4%,respectively).At higher canopy densities,the raters overestimated foliage density,but overall agreement between raters and measured values was good(ρc=0.849 to 0.915),and inter-rater reliability was high(R^(2)=0.910 to 0.953).The provenance from Missouri(MOL),the northernmost provenance,had the lowest canopy density in November,and results show that the higher the latitude of the provenance,the lower the canopy *** on regression,the source provenance latitude explained 0.609 of the variation using image analysis,and0.551 to 0.640 when based on the rater estimates of canopy *** assessment of pecan canopy density due to late season leaf fall for comparing pecan genotypes provides accurate and reliable estimates and could be used in future studies of the whole provenance collection.

关键词： Carya illinoinensis Accuracy Reliability Canopy Foliage density Leaf area density Leaf area index

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About Bilingual Pragmatic Competence

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现代外语 2014年第1期37卷 1-22页

作者： istvan Kecskes State University of New York Albany USA

双语语用能力是双语研究中较少关注的议题。本文主要探讨成年双语者习得的语言及其相关社会-文化如何影响原有的语用知识与能力。作者假定双语者已具备一种由母语主导的语用能力,该能力可根据习得语言的社会-文化要求作出调整。母语语... 详细信息

双语语用能力是双语研究中较少关注的议题。本文主要探讨成年双语者习得的语言及其相关社会-文化如何影响原有的语用知识与能力。作者假定双语者已具备一种由母语主导的语用能力,该能力可根据习得语言的社会-文化要求作出调整。母语语用能力发展与第二语言语用能力发展两者之间存在根本性差异,前者主要受制于社会-文化环境,后者则主要取决于个人的意愿和喜好。母语的语言发展过程与相关社会-文化的积累过程齐头并进,是一种潜意识、自动化、本能的过程,该过程主要依赖于社会-文化环境的性质及其直接接触,个人意愿对于社会技能和知识的获取影响有限。双语语用能力的发展过程则迥然不同,其主要特点是双语者可调控自己对二语的相关社会-文化范式和规约的接受程度。个人可根据自我调控、意识和意愿去调整现有的技能和行为模式,并选择性地获取某些社会技能,从而影响二语语用能力的发展过程。

关键词：双语语用能力社会一文化环境双向语用推导

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Socio-cognitive Approach to Pragmatics

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外国语 2010年第5期33卷 2-20页

作者： istvan Kecskes State University of New York Albany U. S. A

Communication is not as smooth a process as current pragmatic theories depict *** Rapaport's words "We almost always fail .Yet we almost always nearly succeed: This is the paradox of communication(Rapaport,2003:402)." This paper claims that there is a need for an approach that is able to explain this "bumpy road" by analyzing both the positive and negative features of the communicative *** paper presents a socio-cognitive approach(SCA) to pragmatics that takes into account both the societal and individual factors including cooperation and egocentrism that,as claimed here,are not antagonistic phenomena in *** approach is considered an alternative to current theories of pragmatics that do not give an adequate account of what really happens in the communicative *** consider communication an idealistic,cooperation-based,context-dependent process in which speakers are supposed to carefully construct their utterances for the hearer taking into account all contextual factors and hearers do their best to figure out the intentions of the *** approach relies mainly on the positive features of communication including cooperation,rapport and politeness while almost completely ignores the untidy,trial-and-error nature of communication and the importance of prior contexts captured in the individual use of linguistic *** overemphasis on cooperative,societal,contextual factors has led to disregard individual factors such as egocentrism and salience that are as important contributors to the communicative process as cooperation,context and *** socio-cognitive approach is presented as a theoretical framework to incorporate and reconcile two seemingly antagonistic sides of the communicative process and explain the dynamic interplay of prior and actual situational contexts.

关键词： context cooperation egocentrism attention salience

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On Modifications of Continuous and Discrete Maximum Principles for Reaction-Diffusion Problems

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Advances in Applied Mathematics and Mechanics 2011年第1期3卷 109-120页

作者： istvan Farago Sergey Korotov Tamas Szabo Department of Applied Analysis and Computational Mathematics Eotvos Lorand UniversityH–1117BudapestPazmany P.s.1/c.Hungary BCAM-Basque Center for Applied Mathematics Bizkaia Technology ParkBuilding 500E–48160 DerioBasque CountrySpain

In this work,we present and discuss some modifications,in the form of two-sided estimation(and also for arbitrary source functions instead of usual sign-conditions),of continuous and discrete maximum principles for th... 详细信息

关键词： Reaction-diffusion problem maximum principle discrete maximum principle monotone matrix two-sided a priori estimation code validification

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Local color and morphological image feature based vegetation identification and its application to human environment street view vegetation mapping, or how green is our county?

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Geo-Spatial Information Science 2020年第3期23卷 222-236,I0003页

作者： istvan G.Lauko Adam Honts Jacob Beihoff Scott Rupprecht Department of Mathematical Sciences University of Wisconsin-MilwaukeeMilwaukeeWIUSA Lubar School of Business University of Wisconsin-MilwaukeeMilwaukeeWIUSA Scott Rupprecht Limited Liability Company MilwaukeeWIUSA

Measuring the amount of vegetation in a given area on a large scale has long been accomplished using satellite and aerial imaging *** methods have been very reliable in measuring vegetation coverage accurately at the top of the canopy,but their capabilities are limited when it comes to identifying green vegetation located beneath the canopy *** the amount of urban and suburban vegetation along a street network that is partially beneath the canopy has recently been introduced with the use of Google Street View(GSV)images,made accessible by the Google Street View Image *** green vegetation through the use of GSV images can provide a comprehensive representation of the amount of green vegetation found within geographical regions of higher population density,and it facilitates an analysis performed at the *** this paper we propose a fine-tuned color based image filtering and segmentation technique and we use it to define and map an urban green environment *** deployed this image processing method and,using GSV images as a high-resolution GIS data source,we computed and mapped the green index of Milwaukee County,a 3,082 km^(2) urban/suburban county in *** approach generates a high-resolution street-level vegetation estimate that may prove valuable in urban planning and management,as well as for researchers investigating the correlation between environmental factors and human health outcomes.

关键词： Urban environment mapping greenview index panoramic street view urban landscape urban planning

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