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Assessment of Photochemical Reflectance Index as a Tool for Evaluation of Chlorophyll Fluorescence Parameters in Cotton and Peanut Cultivars Under Water Stress Condition

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Agricultural Sciences in China 2010年第5期9卷 662-670页

作者： Shahenshah Yasuda Yoshizumi LI Mao-song isoda akihiro Graduate School of Horticulture Chiba University Chiba 271-8510 Japan Graduate School oflnformatics Tokyo University of Information Sciences Chiba 265-8501 Japan Institute of Environment and Sustainable Development for Agriculture Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China

The relationships between photochemical reflectance index （PRI） and the chlorophyll fluorescence parameters were examined to assess suitability of PRI as a remote-sensing tool for the chlorophyll fluorescence parameters. A greenhouse experiment was conducted using cotton and peanut crops under water stress condition. Five cotton and six peanut cultivars were grown using Andosole soil in pots maintained at two water levels; the control and water stress treatment （WS） of 100 and 50% of the daily transpiration, respectively. Higher non-photochemical quenching （NPQ） was exhibited by peanut than that of cotton by the water stress. On the other hand, the decreases of the actual quantum yield of photosystem II （△F/F＇m） and PRI by the water stress in cotton were larger than those in peanut. There were positively significant correlation coefficients between PRI and △F/F＇m in cotton at noon and in the afternoon including the control and WS. The correlations of PRI with NPQ were negatively significant at noon and in the afternoon for cotton, and in the afternoon for peanut. No clear relationship was found among these parameters in the morning probably due to the diurnal increase in global solar radiation. It was concluded that there would be a possibility to detect the effects of water stress on △F/F＇m and NPQ by PRI with some exceptions, although PRI could not note varietals differences in △F/F＇m and NPQ for each treatment.

关键词： actual quantum yield of photosystem II Arachis hypogaea L. Gossypium hirsutum L. non-photochemicalquenching photochemical reflectance index remote sensing water stress

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Growth and Eco-Physiological Performance of Cotton Under Water Stress Conditions

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Agricultural Sciences in China 2007年第8期6卷 949-955页

作者： WANG Chun-yan isoda akihiro LI Mao-song WANG Dao-long Institute of Environment and Sustainable Development for Agriculture Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China Faculty of Horticulture Chiba University Matsudo Chiba 271-8510 Japan Key Laboratory of Agro-Enviroment ＆ Climate Change Ministry of Agriculture Beijing 100081 P.R. China

A cotton cultivar Xinluzao 8 was grown under four levels of water stress treatments （normal irrigation, slight, mild and severe water stress） from the initial reproductive growth stage in Shihezi, Xinjiang, China, in 2002, to evaluate the growth and eco-physiological performances. Under water stress conditions, the transpiration ability decreased while the leaf temperature increased. Although the relative leaf water content decreased as water stress increased, the differences among the treatments were small, indicating that cotton has high ability in maintaining water in leaf. The stomatal density increased as water stress increased, while the maximum stomatal aperture reduced only in the severest stressed plants. The time of the maximum stomatal aperture was delayed in the mild and severe stressed plants. When severe stress occurred, the stomata were kept open until the transpiration decreased to nearly zero, suggesting that the stomata might not be the main factor in adjusting transpiration in cotton. Cotton plant has high adaptation ability to water stress conditions because of decrease in both stomatal conductance and hydraulic conductance from soil-to-leaf pathway. The actual quantum yield of photosystem Ⅱ （PS Ⅱ） decreased under water stress conditions, while the maximum quantum yield of PS Ⅱ did not vary among treatments, suggesting that PS II would not be damaged by water stress. The total dry weight reduced as water stress increased.

关键词： cotton eco-physiological performance water stress transpiration stomatal aperture

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新疆石河子棉区高密条件下冠层结构和光分布特征

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棉花学报 2006年第4期18卷 223-227页

作者：王春艳礒田昭弘王道龙李茂松阮明艳苏跃中国农业科学院农业环境与可持续研究所北京100081 日本千葉大学園芸学部新疆石河子中亚干旱农业环境研究所石河子831000

对新疆石河子5个棉花主栽品种全生育期的干物质积累、生长参数、产量和盛铃期的冠层结构、光渗透能力和光分布等进行了测量分析。结果表明,作物生长速率、铃生长率以及子棉产量等均与总受光量呈正相关关系,净同化率则与单位叶面积受光... 详细信息

对新疆石河子5个棉花主栽品种全生育期的干物质积累、生长参数、产量和盛铃期的冠层结构、光渗透能力和光分布等进行了测量分析。结果表明,作物生长速率、铃生长率以及子棉产量等均与总受光量呈正相关关系,净同化率则与单位叶面积受光量呈正相关关系,说明受光量是决定干物质生产和产量的重要因素;单位叶面积受光量即光的渗透能力与单位土地面积总受光量呈正相关关系、而与LAI呈负相关关系,说明冠层结构也是影响冠层受光量的主要因素;总受光量与LAI呈显著的正相关关系,说明LAI最大值并未达到最适宜水平。新疆地区棉花高产棉田的主要特征可以总结为叶数少、叶片大、冠层结构为椭圆形。由于叶面积指数尚未达到最适宜水平,增加种植密度可能是提高产量的一个途径。

关键词：新疆棉花生长参数受光量冠层结构

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Weak-acid biochar catalyst prepared from mechanochemically-activated biomass and humic acid for production of 5-hydroxymethylfurfural

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Biochar 2022年第1期4卷 490-502页

作者： Haixin Guo Sohei Ogawa Yukiya isoda Feng Shen Richard Lee Smith Jr Ministry of Agriculture and Rural Affairs Agro-Environmental Protection InstituteNo.31 Fukang RoadNankai DistrictTianjin 300191China Graduate School of Environmental Studies Tohoku UniversityAramaki Aza Aoba 6-6-11AobaSendai 980-8579Japan

Many types of sustainably-prepared functional biochar catalysts are needed to transform biomass substrates into platform *** this work,weak-acid biochar catalysts were prepared by mix ball-milled(10 min,isothermal,room temperature)cherry blossom tree leaf biomass(BM)with humic acid(H)prior to carbonization of samples for 1 h under nitrogen *** of the as-prepared materials(HBM-carbonization temperature in℃)showed that humic acid activated the biochars and increased their number of oxygen-containing functional(-COOH,OH)*** weak-acid biochars were applied as heterogeneous catalysts for dehydration of fructose to 5-hydroxymethylfurfural(5-HMF),where it was found that biochar catalyst,HBM-400,gave 100%fructose conversion and 77.5%5-HMF yields in an ionic liquid(1-butyl-3-methylimidazolium chloride)reaction system(140℃,60 min reaction time).As-prepared HBM-400 biochars exhibited constant catalytic activity for fructose dehydration with repeated use without any type of reactivation treatment and were stable for 5 *** acid is an effective additive for preparing weak-acid catalytic materials from waste biomass.

关键词： Humic acid Mixing ball milling Oxygen-containing functional group Weak-acid biochar

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Impelling force and current challenges by chemicals in somatic cell reprogramming and expansion beyond hepatocytes

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World Journal of Stem Cells 2019年第9期11卷 650-665页

作者： Jian-Yun Ge Yun-Wen Zheng Li-Ping Liu Hiroko isoda Tatsuya Oda Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Faculty of MedicineUniversity of TsukubaTsukubaIbaraki 305-8575Japan Institute of Regenerative Medicine and Affiliated Hospital Jiangsu UniversityZhenjiang 212001Jiangsu ProvinceChina Department of Regenerative Medicine School of MedicineYokohama City UniversityYokohama 236-0004Japan Faculty of Life and Environmental Sciences University of TsukubaTsukubaIbaraki 305-8572Japan

In the field of regenerative medicine,generating numerous transplantable functional cells in the laboratory setting on a large scale is a major ***,the in vitro maintenance and expansion of terminally differentiated cells are challenging because of the lack of specific environmental and intercellular signal stimulations,markedly hindering their therapeutic ***,the generation of stem/progenitor cells or functional cells with effective proliferative potential is markedly in demand for disease modeling,cell-based transplantation,and drug *** the potent genetic manipulation of transcription factors,integration-free chemically defined approaches for the conversion of somatic cell fate have garnered considerable attention in recent *** review aims to summarize the progress thus far and discuss the advantages,limitations,and challenges of the impact of full chemicals on the stepwise reprogramming of pluripotency,direct lineage conversion,and direct lineage expansion on somatic *** to the current chemical-mediated induction,reprogrammed pluripotent stem cells with reproducibility difficulties,and direct lineage converted cells with marked functional deficiency,it is imperative to generate the desired cell types directly by chemically inducing their potent proliferation ability through a lineagecommitted progenitor state,while upholding the maturation and engraftment capacity posttransplantation in *** with the comprehensive understanding of the mechanism of chemical drives,as well as the elucidation of specificity and commonalities,the precise manipulation of the expansion for diverse functional cell types could broaden the available cell sources and enhance the cellular function for clinical application in future.

关键词： Chemical induction Pluripotent reprogramming Direct lineage conversion Direct lineage expansion Hepatocyte expansion Cell fate specificity Transcriptional memory In vivo induction

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采用Caco-2和PC12细胞检测有机磷农药的细胞毒性

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环境科学研究 2003年第2期16卷 47-50页

作者：汝少国 isoda Hiroko 邴欣中国海洋大学海洋生命学院山东青岛266003 筑波大学农林工学系

建立了采用哺乳动物细胞系Caco-2和PC12细胞检测有机磷农药细胞毒性的新方法。1 0μmol L甲基对硫磷、敌敌畏、氧乐果、辛硫磷处理经过单层培养的Caco-2细胞,随着农药处理时间的延长,Caco-2细胞跨上皮细胞电阻抗值逐渐降低,处理60min时... 详细信息

建立了采用哺乳动物细胞系Caco-2和PC12细胞检测有机磷农药细胞毒性的新方法。1 0μmol L甲基对硫磷、敌敌畏、氧乐果、辛硫磷处理经过单层培养的Caco-2细胞,随着农药处理时间的延长,Caco-2细胞跨上皮细胞电阻抗值逐渐降低,处理60min时,辛硫磷和甲基对硫磷对Caco-2细胞具有明显的透过性,约是正常组的1 5倍,4种农药对Caco-2细胞透过性由大到小的顺序为:甲基对硫磷,辛硫磷,敌敌畏,氧乐果;0 01μmol L的甲基对硫磷和辛硫磷使PC12细胞乙酰胆碱酯酶活性迅速降低,随着处理浓度的增加酶活性降低不明显;而小于1 0μmol L的氧乐果和敌敌畏呈现渐进降低的趋势,当浓度大于1 0μmol L时酶活性降低比较显著。

关键词：有机磷农药 Caco-2 PC12 细胞毒性跨上皮细胞电阻抗值乙酰胆碱酯酶

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Evaluation of safety for hepatectomy in a novel mouse model with nonalcoholic-steatohepatitis

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World Journal of Gastroenterology 2018年第15期24卷 1622-1631页

作者： Yusuke Ozawa Takafumi Tamura Yohei Owada Yoshio Shimizu Akira Kemmochi Katsuji Hisakura Takashi Matsuzaka Hitoshi Shimano Hiroko isoda Nobuhiro Ohkohchi Department of Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Faculty of Medicine University of Tsukuba Department of Endocrinology and Metabolism Faculty of Medicine University of Tsukuba Faculty of Life and Environmental Sciences University of Tsukuba

AIM To investigate whether the liver resection volume in a newly developed nonalcoholic steatohepatitis(NASH) model influences surgical *** For establishment of a NASH model, mice were fed a high-fat diet for 4 wk, administered CCl_4 for the last 2 wk, and administered T0901317 for the last 5 d. We divided these mice into two groups: A 30% partial hepatectomy(PH) of NASH liver group and a 70% PH of NASH liver group. In addition, a 70% PH of normal liver group served as the control. Each group was evaluated for survival rate, regeneration, apoptosis, necrosis and DNA expression after *** In the 70% PH of NASH group, the survival rate was significantly decreased compared with that in the control and 30% PH of NASH groups(P < 0.01). 10 of 32 mice in the NASH 70% PH group died within 48 h after PH. Serum aspartate aminotransferase(AST) levels and total bilirubin(T-Bil) in the NASH 70% PH group were significantly higher than the levels in the other two groups(AST: P < 0.05, T-Bil: P < 0.01). In both PH of NASH groups, signaling proteins involved in regeneration were expressed at lower levels than those in the control group(P < 0.01). The 70% PH of NASH group also exhibited a lower number of Ki-67-positive cells and higher rates of apoptosis and necrosis than the NASH 30% PH group(P < 0.01). In addition, DNA microarray assays showed differences in gene expression associated with cell cycle arrest and *** The function of the residual liver is impaired in fatty liver compared to normal liver. A larger residual volume is required to maintain liver functions in mice with NASH.

关键词： Hepatectomy Liver regeneration Residual liver Liver proliferation Nonalcoholic steatohepatitis

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<sup>31</sup>P MR Spectroscopy with 3D Chemical-Shift Imaging Detects Changes in Levels of Phosphorus Metabolites Due to Saliva Secretion in Human Parotid Glands

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Open Journal of Medical Imaging 2020年第1期10卷 42-51页

作者： Toshiyuki Sato Hiroyoshi isoda Kaori Togashi Department of Diagnostic Imaging and Nuclear Medicine Graduate School of Medicine Kyoto University Shogoin-Kawaharacho Sakyo-ku Kyoto Japan

.Objectives: To measure phosphorus metabolites in human parotid glands by 31P-MRS using three-dimensional chemical-shift imaging (3D-CSI), and ascertain whether this method can capture changes in adenosine triphosphate (ATP) and phosphocreatine (PCr) levels due to saliva secretion. Study Design: The parotid glands of 20 volunteers were assessed by 31P-MRS using 3D-CSI on 3T MRI. After obtaining a first (baseline) measurement, the participants took vitamin-C tablets and measurements were obtained twice more, in a continuous manner. The peak area ratios of PCr and β-ATP were evaluated. Results: A high proportion of PCr (0.26 ± 0.08) and ATP (α: 0.16 ± 0.06;β: 0.27 ± 0.06;γ: 0.21 ± 0.03) was noted at baseline. A significant decrease in β-ATP was observed between baseline (“pre”;0.27 ± 0.06) and the first scan after vitamin-C stimulation (“post-1”;0.19 ± 0.07, p < 0.05). There was a near-significant decrease in PCr between pre (0.26 ± 0.08) and post-1 (0.23 ± 0.06, p = 0.074). Conclusions: 31P-MRS with 3D-CSI can assess the major phosphorus metabolites in human parotid glands and capture changes in their levels due to saliva secretion. This technique is simple, non-invasive, and provides new information regarding saliva secretion.

关键词： 31P-MRS 3D-CSI Parotid Gland

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Anti-stress Effects of Tunisian Cymbopogon schoenanthus L. Ethanol Extract and Some of Its Active Compounds

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Journal of Agricultural Science and Technology(A) 2017年第2期7卷 91-99页

作者： Mahmoud Ben Othman Mohamed Neffati Hiroko isoda Alliance for Research on North Africa (ARENA) University of Tsukuba 1-1-1 Tennodai Tsukuba City Ibaraki 305-85 72 Japan Faculty of Life and Environmental Sciences University of Tsukuba 1-1-1 Tennodai Tsukuba City lbaraki 305-8572 Japan Range Ecology Laboratory Arid Land Institute of Medenine Medenine 4119 Tunisia

Many antioxidant rich phytochemical extracts are attracting interest as stress adaptogens. In this regard, several plants are used traditionally and clinically for the management of neurologic disorders. Here, the anti-stress properties of the ethanol extract of medicinal plant Cymbopogon schoenanthus （CSEE）, which grows wild in Southern Tunisia, were investigated, as well as the effects of some of the extracted active compounds on H202-induced cytotoxicity, overproduction of reactive oxygen species （ROS） and adenosine triphosphate （ATP） depletion in human neuroblastoma SH-SYSY cells. Whereas fluorescence intensity due to DCFH-DA （a marker of ROS production） in H2O2-treated SH-SY5Y cells was greater than that in untreated control cells, pretreatrnent with CSEE and its active compounds ameliorated H2O2-induced ROS production. Moreover, H2O2 at 150 μM significantly decreased intracellular ATP levels in SH-SY5Y cells, and pretreatment with CSEE and its active compounds buffered this effect. These in vitro results demonstrate the potential of CSEE to protect against stress-associated disorders.

关键词： ATP levels H2O2 medicinal plant reactive oxygen species stress.

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17β-雌二醇对雄性金鱼卵黄原蛋白的诱导作用

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水产学报 2004年第3期28卷 236-240页

作者：邴欣,汝少国,isoda Hiroko,姜明,王蔚,王诗红中国海洋大学海洋生命学院中国海洋大学海洋生命学院筑波大学农林工学系中国海洋大学海洋生命学院中国海洋大学海洋生命学院中国海洋大学海洋生命学院山东青岛　266003 山东青岛　266003 日本筑波　305 山东青岛　266003 山东青岛　266003 山东青岛　266003

采用腹腔注射17β-雌二醇的方法诱导雄性金鱼卵黄原蛋白产生,注射浓度为0.05mg·g-1BW,诱导2周后取尾静脉血,离心分离血浆,进行血浆常规聚丙烯酰胺凝胶电泳,通过对卵黄原蛋白特性基团磷、脂和糖蛋白的染色,确定了卵黄原蛋白在电泳图谱... 详细信息

采用腹腔注射17β-雌二醇的方法诱导雄性金鱼卵黄原蛋白产生,注射浓度为0.05mg·g-1BW,诱导2周后取尾静脉血,离心分离血浆,进行血浆常规聚丙烯酰胺凝胶电泳,通过对卵黄原蛋白特性基团磷、脂和糖蛋白的染色,确定了卵黄原蛋白在电泳图谱上的位置,开发了一种简便、高效的定性卵黄原蛋白的聚丙烯酰胺凝胶电泳法。电泳结果表明,在0.05mg·g-1BW的注射浓度下,2周后17β-雌二醇诱导了雄性金鱼卵黄原蛋白产生,并通过ELISA检测卵黄原蛋白的平均含量为690.2ng·mL-1,与对照组雄性金鱼平均含量为10.7ng·mL-1的差异极显著(P<0.01),比雌性对照组检出量285.5ng·mL-1高1倍多;17β-雌二醇诱导组雄鱼血浆钙离子和血总蛋白含量明显增加。

关键词：金鱼 17β-雌二醇卵黄原蛋白

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